IVF/ICSI周期中≥3PN胚胎用于囊胚培养方案优化的探讨
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摘要
目的探讨单个囊胚培养滴中培养的胚胎数目和囊胚培养滴体积对囊胚形成的影响。方法选择助孕治疗过程中细胞数≥6、碎片≤30%、原核数≥3的多原核(≥3PN)异常受精胚胎进行囊胚培养,将授精第3日3PN胚胎随机分为1、2、3、4个的组合分别置于体积为30μl的单个囊胚培养滴中,或将授精第3日3PN胚胎随机置于体积为10、20、30、40、50μl的囊胚培养滴中培养,观察囊胚形成情况并进行比较。结果不同胚胎数目组间的3期以上囊胚或早期以上囊胚的形成率比较差异均无统计学意义(P均> 0.05)。不同囊胚培养滴体积组间的3期以上囊胚和早期以上囊胚形成率比较差异亦无统计学意义(P均> 0.05)。结论单个囊胚培养滴中培养的胚胎数目和培养滴体积对囊胚形成无影响。
Objective To investigate the effect of the number of embryos cultured in a single blastocyst medium droplet and the volume of blastocyst medium droplet on blastocyst formation. Methods With the informed consents of the patients, the abnormally fertilized embryos( ≥ 3 PN embryos) with pronuclear number ≥ 3 from July 2016 to June 2018 were utilized for blastocyst culture. The inclusion criteria were cell number ≥ 6 and fragment ≤ 30%. The 3 PN embryos on the 3 rd day of insemination were randomly divided into one, two, three, and four combinations and cultured in a single blastocyst medium droplet in a volume of 30 μl. Subsequently, the 3 PN embryos on the 3 rd day of insemination were randomly cultured in 10 μl, 20 μl, 30 μl, 40 μl, and 50 μl of blastocyst medium droplet. The formation rate of blastocysts was observed and statistically compared. Results The number of embryos did not affect the formation rate of ≥ stage 3 or early blastocysts(both P > 0.05). The difference was not statistically significant in the formation rates of ≥ stage 3 blastocysts and ≥ early blastocysts in groups of different volume of culture droplets(both P > 0.05). Conclusion The number of embryos cultured in a single blastocyst medium droplet and the volume of culture droplets exerts no effect on the blastocyst formation.
Objective To investigate the effect of the number of embryos cultured in a single blastocyst medium droplet and the volume of blastocyst medium droplet on blastocyst formation. Methods With the informed consents of the patients, the abnormally fertilized embryos( ≥ 3 PN embryos) with pronuclear number ≥ 3 from July 2016 to June 2018 were utilized for blastocyst culture. The inclusion criteria were cell number ≥ 6 and fragment ≤ 30%. The 3 PN embryos on the 3 rd day of insemination were randomly divided into one, two, three, and four combinations and cultured in a single blastocyst medium droplet in a volume of 30 μl. Subsequently, the 3 PN embryos on the 3 rd day of insemination were randomly cultured in 10 μl, 20 μl, 30 μl, 40 μl, and 50 μl of blastocyst medium droplet. The formation rate of blastocysts was observed and statistically compared. Results The number of embryos did not affect the formation rate of ≥ stage 3 or early blastocysts(both P > 0.05). The difference was not statistically significant in the formation rates of ≥ stage 3 blastocysts and ≥ early blastocysts in groups of different volume of culture droplets(both P > 0.05). Conclusion The number of embryos cultured in a single blastocyst medium droplet and the volume of culture droplets exerts no effect on the blastocyst formation.
引文
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[3]郑炜炜,谭玉梅,祝晓丽,陈瑞玲,谭颖,姜荣华,刘善文,吴铮,宋革.不同精子来源及授精方式对剩余胚胎继续囊胚培养结局的影响.生殖与避孕, 2016, 36(10):845-851.
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[7]Tao T, Robichaud A, Mercier J, Ouellette R. Influence of group embryo culture strategies on the blastocyst development and pregnancy outcome. J Assist Reprod Genet,2013,30(1):63-68.
[8]Sun B, Yu W, Wang F, Song W, Jin H, Sun Y. Effects of group culture on the development of discarded human embryos and the construction of human embryonic stem cell lines. J Assist Reprod Genet,2014,31(10):1369-1376.
[9]Minasi MG, Fabozzi G, Casciani V, Lobascio AM, Colasante A,Scarselli F, Greco E. Improved blastocyst formation with reduced culture volume:comparison of three different culture conditions on 1128 sibling human zygotes. J Assist Reprod Genet,2015,32(2):215-220.
[10]De Munck N, Santos-Ribeiro S, Mateizel I, Verheyen G.Reduced blastocyst formation in reduced culture volume. J Assist Reprod Genet,2015,32(9):1365-1370.
[11]Dai SJ, Xu CL, Wang J, Sun YP, Chian RC. Effect of culture medium volume and embryo density on early mouse embryonic development:tracking the development of the individual embryo.J Assist Reprod Genet,2012,29(7):617-623.
[2]Oron G, Sokal-Arnon T, Son WY, Demirtas E, Buckett W,Zeadna A, Holzer H, Tulandi T. Extended embryo culture is not associated with increased adverse obstetric or perinatal outcome.Am J Obstet Gynecol,2014,211(2):165.e1-e7.
[3]郑炜炜,谭玉梅,祝晓丽,陈瑞玲,谭颖,姜荣华,刘善文,吴铮,宋革.不同精子来源及授精方式对剩余胚胎继续囊胚培养结局的影响.生殖与避孕, 2016, 36(10):845-851.
[4]鲁振宇,张云山,糜若然.胚胎发育对囊胚形成的影响.天津医药杂志,2010,38(4):257-259.
[5]任新玲,刘群,艾继辉,章汉旺.胚胎卵裂速度异常对囊胚形成的影响.中国妇幼保健,2012,27(7):1061-1063.
[6]刁英,杨智敏,谭兵兵.移植早期分裂受精卵对IVF-ET或ICSI-ET结局的影响——附259个周期分析.新医学, 2009,40(4):241-243.
[7]Tao T, Robichaud A, Mercier J, Ouellette R. Influence of group embryo culture strategies on the blastocyst development and pregnancy outcome. J Assist Reprod Genet,2013,30(1):63-68.
[8]Sun B, Yu W, Wang F, Song W, Jin H, Sun Y. Effects of group culture on the development of discarded human embryos and the construction of human embryonic stem cell lines. J Assist Reprod Genet,2014,31(10):1369-1376.
[9]Minasi MG, Fabozzi G, Casciani V, Lobascio AM, Colasante A,Scarselli F, Greco E. Improved blastocyst formation with reduced culture volume:comparison of three different culture conditions on 1128 sibling human zygotes. J Assist Reprod Genet,2015,32(2):215-220.
[10]De Munck N, Santos-Ribeiro S, Mateizel I, Verheyen G.Reduced blastocyst formation in reduced culture volume. J Assist Reprod Genet,2015,32(9):1365-1370.
[11]Dai SJ, Xu CL, Wang J, Sun YP, Chian RC. Effect of culture medium volume and embryo density on early mouse embryonic development:tracking the development of the individual embryo.J Assist Reprod Genet,2012,29(7):617-623.