健脾2号口服液对小鼠肠道菌群影响的实验研究
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摘要
目的:评价健脾2号口服液(JP)对小鼠正常肠道菌群的调节作用。方法:将50只雌性BALB/c小鼠随机分为对照组、低聚半乳糖组(OG, 0.50mg/kg)和健脾2号口服液高(JP-H, 6.00mg/kg)、中(JP-M, 3.00mg/kg)、低(JP-L, 1.50mg/kg)剂量组,给药前各组均无菌采取小鼠直肠粪便0.1g,再每组灌胃相应药物(1次/d),空白组给予蒸馏水,连续14d。末次给药后24h,同法取粪便,将两次所取粪便培养后,以生化反应、革兰氏染色镜检、菌落形态等鉴定及计录菌落数,并算出每克湿便中所含肠道双歧杆菌、乳杆菌、肠球菌、肠杆菌、产气荚膜梭菌的菌落数,取对数后进行统计。结果:给药前至给药后14d,各组小鼠体重增加趋势基本相同,各个时间点体重无显著性差异(P>0.05);灌胃14d,与对照组比较,给药后OG组、JP三剂量组乳杆菌和双歧杆菌数量有显著性增加(P<0.01);JP三剂量组肠球菌和肠杆菌数量均有显著性减少(P<0.01);而OG、JP三剂量组产气荚膜梭菌数量均有显著性减少(P<0.01)。结论:健脾2号口服液具有明显的调节肠道菌群作用,可用于脾气亏虚、消化不良的治疗。
Objective: To observe the regulating effect of Jianpi NO.2 oral liquid(JP) on intestinal microflora in mice. Methods: 50 female BALB/c mice were randomly divided into control group, OG group(0.50 mg/kg) and JP-H(6.00 mg/kg), JP-M(3.00 mg/kg), JP-L(1.50 mg/kg) groups. Before administration, 0.1 g of rectal feces were collected under sterile environment, and each group of mice were given corresponding drugs(qd) for 14 days. After 24 h of the last administration, collected rectal feces in the same way and cultured, identified and counted the colonies by biochemical reactions, gram staining microscopy and colonial morphology, and calculated the intestinal bifidobacterium, lactobacillus, enterococcus, Enterobacter and Clostridium perfringens in each gram of wet stool, and counted after logarithm. Results: After 14 days of administration, the trend of weight gain in each group was basically same, and there was no significant difference in body weight at each time point(P>0.05); compared with control group,after administration, Lactobacillus and Bifidobacterium increased significantly in OG group and JP three-dose groups(P<0.01); Enterococcus and Enterobacter decreased significantly in JP three-dose groups(P<0.01); and Clostridium perfringens in OG and JP groups decreased significantly(P<0.01). Conclusion: Jianpi NO.2 oral liquid has significant regulating effect on intestinal microflora, which can be used in treatment of spleen qi deficiency and indigestion.
Objective: To observe the regulating effect of Jianpi NO.2 oral liquid(JP) on intestinal microflora in mice. Methods: 50 female BALB/c mice were randomly divided into control group, OG group(0.50 mg/kg) and JP-H(6.00 mg/kg), JP-M(3.00 mg/kg), JP-L(1.50 mg/kg) groups. Before administration, 0.1 g of rectal feces were collected under sterile environment, and each group of mice were given corresponding drugs(qd) for 14 days. After 24 h of the last administration, collected rectal feces in the same way and cultured, identified and counted the colonies by biochemical reactions, gram staining microscopy and colonial morphology, and calculated the intestinal bifidobacterium, lactobacillus, enterococcus, Enterobacter and Clostridium perfringens in each gram of wet stool, and counted after logarithm. Results: After 14 days of administration, the trend of weight gain in each group was basically same, and there was no significant difference in body weight at each time point(P>0.05); compared with control group,after administration, Lactobacillus and Bifidobacterium increased significantly in OG group and JP three-dose groups(P<0.01); Enterococcus and Enterobacter decreased significantly in JP three-dose groups(P<0.01); and Clostridium perfringens in OG and JP groups decreased significantly(P<0.01). Conclusion: Jianpi NO.2 oral liquid has significant regulating effect on intestinal microflora, which can be used in treatment of spleen qi deficiency and indigestion.
引文
[1]唐甜,索化夷,李键,等.膳食营养与宿主遗传通过肠道菌群调节能量代谢研究进展[J].食品与机械,2019,35(1):1-11.
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[12]蔚晓霞,刘占举,胡艺,等.炎症性肠病患者的肠道菌群分布与血清TNF-α与IL-6水平的相关性[J].现代生物医学进展,2017,17(16):3076-3078,3102.
[13]纪丽丽,祁根兄,王传宝,等.中草药提取物对肉鸡生长性能、器官发育、免疫功能及肠道菌群的影响[J].中国饲料,2018,(22):32-36.
[14]栾英桥,杨佳幸,陶嫦立,等.肠道菌群影响肠道细胞免疫的研究进展[J].中国免疫学杂志,2018,34(11):1734-1737,1742.
[15]尹志婷.原花青素B2干预D-半乳糖致衰老小鼠的代谢组学与肠道菌群研究[D].上海应用技术大学,2018.
[16]陆菊仙.肠道正常菌群与机体肿瘤发生的相互关系[J].中国微生态学杂志,1992,(1):46-51.
[17]徐春晖,宿扬,田志颖,等.恶性血液病患者合并产气荚膜梭菌血症1例病例报告并文献回顾[J].中国感染控制杂志,2018,17(3):270-272.
[18]彭颖,金晶,杨静玉,等.3种健脾补气方药对脾气虚证大鼠肠道菌群的影响[J].中国中药杂志,2008,33(21):2530-2534.
[19]马祥雪,王凤云,符竣杰,等.基于肠道菌群的中医健脾方剂作用机制的研究现状与思考[J].中国实验方剂学杂志,2017,23(5):210-215.
[20]王卓,彭颖,李晓波.四君子汤对两种脾虚模型大鼠肠道菌群紊乱的影响[J].中国中西医结合杂志,2009,29(9):825-829.
[21]陈晓刚.小儿再发性腹痛的脾胃湿热证与肠道微生态关系的研究[D].广州中医药大学,2007.
[22]张玉佩,杨钦河,邓远军,等.中焦湿热证与胃肠道微生态关系刍议[J].中医杂志,2016,57(24):2094-2096.
[23]曾宏亮,肖满,陈波,等.复方苍术方调节肠道菌群及肠道保护作用研究[J].现代食品科技,2018,34(1):1-6.
[24]包辰.薏苡仁抗性淀粉结构特性及其对肠道菌群调节机制的研究[D].福建农林大学,2017.
[25]杨春佳,苏德望,王跃生,等.金银花对梗阻性黄疸大鼠肠道菌群失调的调整作用[J].中国现代医生,2012,50(24):3-4.
[26]杨春佳,苏德望,王跃生,等.金银花水提物对肠道微生态失调大鼠的调整作用[J].中国微生态学杂志,2012,24(2):132-133.
[27]姚小华,唐立,高菲,等.山银花对小鼠肠道菌群失衡的调节作用[J].中国微生态学杂志,2014,26(8):886-888.
[28]沈定树,郑静.益生元与肠道微生态[J].中国微生态学杂志,2013,25(6):742-744.
[2]Maukonen J,Kolho K L,Paasela M,et al.Altered Fecal Microbiota in Paediatric Inflammatory Bowel Disease[J].J Crohns Colitis,2015,9(12):1088-1095.
[3]王生,黄晓星,余鹏飞,等.肠道菌群失调与结肠癌发生发展之间关系的研究进展[J].中国药理学通报,2014,(8):1045-1049.
[4]Reynolds LA,Finlay BB,Maizels RM.Cohabitation in the Intestine:Interactions among Helminth Parasites,Bacterial Microbiota,and Host Immunity[J].J Immunol,2015,195(9):4059-4066.
[5]Mcphee JB,Schertzer JD.Immunometabolism of obesity and diabetes:microbiota link compartmentalized immunity in the gut to metabolic tissue inflammation[J].Clin Sci(Lond),2015,129(12):1083-1096.
[6]曹战江,于健春,康维明,等.肥胖症肠道菌群与炎症的研究进展[J].中国医学科学院学报,2013,35(4):462-465.
[7]单体栋.低聚半乳糖对急进高原大鼠肠道菌群影响的分子生物学实验研究[D].兰州大学,2012.
[8]陈远东,卢红艳,常明,等.低聚半乳糖对早产大鼠肠道菌群的调节作用[J].重庆医学,2017,46(8):1015-1016,1019.
[9]王尤丽.低聚半乳糖调节小鼠肠道菌群的作用[D].郑州大学,2012.
[10]鄢伟伦,王帅帅,任霞.白术对小鼠肠道菌群调节作用的实验研究[J].山东中医杂志,2011,30(6):417-419.
[11]李旻.人体肠道菌群结构与宿主代谢的相关性研究[D].上海交通大学,2009.
[12]蔚晓霞,刘占举,胡艺,等.炎症性肠病患者的肠道菌群分布与血清TNF-α与IL-6水平的相关性[J].现代生物医学进展,2017,17(16):3076-3078,3102.
[13]纪丽丽,祁根兄,王传宝,等.中草药提取物对肉鸡生长性能、器官发育、免疫功能及肠道菌群的影响[J].中国饲料,2018,(22):32-36.
[14]栾英桥,杨佳幸,陶嫦立,等.肠道菌群影响肠道细胞免疫的研究进展[J].中国免疫学杂志,2018,34(11):1734-1737,1742.
[15]尹志婷.原花青素B2干预D-半乳糖致衰老小鼠的代谢组学与肠道菌群研究[D].上海应用技术大学,2018.
[16]陆菊仙.肠道正常菌群与机体肿瘤发生的相互关系[J].中国微生态学杂志,1992,(1):46-51.
[17]徐春晖,宿扬,田志颖,等.恶性血液病患者合并产气荚膜梭菌血症1例病例报告并文献回顾[J].中国感染控制杂志,2018,17(3):270-272.
[18]彭颖,金晶,杨静玉,等.3种健脾补气方药对脾气虚证大鼠肠道菌群的影响[J].中国中药杂志,2008,33(21):2530-2534.
[19]马祥雪,王凤云,符竣杰,等.基于肠道菌群的中医健脾方剂作用机制的研究现状与思考[J].中国实验方剂学杂志,2017,23(5):210-215.
[20]王卓,彭颖,李晓波.四君子汤对两种脾虚模型大鼠肠道菌群紊乱的影响[J].中国中西医结合杂志,2009,29(9):825-829.
[21]陈晓刚.小儿再发性腹痛的脾胃湿热证与肠道微生态关系的研究[D].广州中医药大学,2007.
[22]张玉佩,杨钦河,邓远军,等.中焦湿热证与胃肠道微生态关系刍议[J].中医杂志,2016,57(24):2094-2096.
[23]曾宏亮,肖满,陈波,等.复方苍术方调节肠道菌群及肠道保护作用研究[J].现代食品科技,2018,34(1):1-6.
[24]包辰.薏苡仁抗性淀粉结构特性及其对肠道菌群调节机制的研究[D].福建农林大学,2017.
[25]杨春佳,苏德望,王跃生,等.金银花对梗阻性黄疸大鼠肠道菌群失调的调整作用[J].中国现代医生,2012,50(24):3-4.
[26]杨春佳,苏德望,王跃生,等.金银花水提物对肠道微生态失调大鼠的调整作用[J].中国微生态学杂志,2012,24(2):132-133.
[27]姚小华,唐立,高菲,等.山银花对小鼠肠道菌群失衡的调节作用[J].中国微生态学杂志,2014,26(8):886-888.
[28]沈定树,郑静.益生元与肠道微生态[J].中国微生态学杂志,2013,25(6):742-744.