赖氨酸表面修饰PVDF纳米纤维膜及其对胆红素的吸附性能
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摘要
为制备具有高通量、低传质阻力及高吸附容量的亲和膜,利用溶液喷射纺丝技术制备了聚偏氟乙烯(PVDF)纳米纤维膜,对其进行活化偶联赖氨酸亲和配基后制得了新型纳米纤维亲和膜,探讨了温度、时间和pH值对其吸附胆红素的影响.结果表明:所得纤维直径范围为50~250 nm,纤维呈三维卷曲形态,杂乱堆积形成蓬松高孔隙率的纳米纤维膜,水通量可达到0.42 mL/(cm~2·s);经赖氨酸表面修饰后,对胆红素具有良好的吸附性能,最大吸附量可达378.69 mg/g.
In order to prepare the affinity membrane with the advantages of high flux, low mass transfer resistance and high adsorption capacity, polyvinylidene fluoride(PVDF) nanofiber membrane was fabricated using solution blowing process and lysine was further immobilized onto its surface for affinity adsorption of bilirubin consideration of effects of time, temperature and pH value. The results showed that the nanofibers mostly ranged from 50 nm to 250 nm in diameter, and they were distributed randomly to form a three-dimensional mat with high porosity and high flux of 0.42 mL/(cm~2·s). Lysine modified nanofibers showed affinity adsorption of bilirubin with high adsorption capacity of 378.69 mg/g.
In order to prepare the affinity membrane with the advantages of high flux, low mass transfer resistance and high adsorption capacity, polyvinylidene fluoride(PVDF) nanofiber membrane was fabricated using solution blowing process and lysine was further immobilized onto its surface for affinity adsorption of bilirubin consideration of effects of time, temperature and pH value. The results showed that the nanofibers mostly ranged from 50 nm to 250 nm in diameter, and they were distributed randomly to form a three-dimensional mat with high porosity and high flux of 0.42 mL/(cm~2·s). Lysine modified nanofibers showed affinity adsorption of bilirubin with high adsorption capacity of 378.69 mg/g.
引文
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[21]SHI W,ZHANG F,ZHANG G,et al.Polylysine-immobilized affinity nylon membrane used for bilirubin adsorption[J].Molecular Simulation,2003,29(12):787-790.
[22]SHI W,ZHANG F,ZHANG G.Adsorption of bilirubin with polylysine carrying chitosan-coated nylon affinity membranes[J].Journal of Chromatography B,2005,819(2):301-306.
[23]DENIZLI A,KOCAKULAK M,PISKIN E.Bilirubin removal from human plasma in a packed-bed column system with dyeaffinity microbeads[J].Journal of Chromatography B Biomedical Sciences&Applications,1998,707(1/2):25-31.
[24]WANG W W,ZHANG H,ZHANG Z F,et al.Amine-functionalized PVA-co-PE nanofibrous membrane as affinity membrane with high adsorption capacity for bilirubin[J].Colloids and Surfaces B:Biointerfaces,2017,150:271-278.
[25]MCDONAGH A F,PHIMISTER A,BOIADJIEV S E,et al.Dissociation constants of carboxylic acids by 13C-NMR in DMSO water[J].Tetrahedron Letters,1999,40(49):8515-8518.
[2]LALLI E,SARTI G C,BOI C.Effect of the spacer arm on non-specific binding in membrane affinity chromatography[J].Mrs Communications,2018,8:65-70
[3]闵明华,石建高,马海有,等.纳米纤维亲和膜应用研究进展[J].合成纤维,2013,42:20-23.MIN M H,SHI J G,MA H Y,et al.Progress of nanofibrous affinity membranes[J].Synthetic Fiber in China,2013,42:20-23(in Chinese).
[4]MA Z,RAMAKRISHNA S.Electrospun regenerated cellulose nanofiber affinity membrane functionalized with protein A/G for IgG purification[J].Journal of Membrane Science,2008,319(1/2):23-28.
[5]MA Z,KOTAKI M,RAMAKRISHNA S.Electrospun cellulose nanofiber as affinity membrane[J].Journal of Membrane Science,2005,265(1/2):115-123.
[6]ZHUANG X P,SHI L,JIA K,et al.Solution blown nanofibrous membrane for microfiltration[J].Jour nal of Membrane Science,2013,429:66-70.
[7]SHI L,ZHUANG X P,TAO X X,et al.Solution blowing nylon6 nanofiber mats for air filtration[J].Fibers and Polymers,2013,14(9):1485-1490.
[8]MA C F,GAO Q,ZHOU J.Facile one-pot synthesis of magnetic nitrogen-doped porous carbon for high-performance bilirubin removal from BSA-rich solution[J].Rsc Advances,2017,7(4):2081-2091.
[9]鞠佳,聂飞,段志军,等.亲和膜配基的结构和密度对胆红素吸附的影响[J].化工学报,2013,64(1):303-310.JU J,NIE F,DUAN Z J,et al.Effect of ligand composition and lingand densith of affinity membrane on bilirubin removal[J].CIESC Joural,2013,64(11):303-310(in Chinese).
[10]薛茂强,石巍,张其清.新型亲和膜的制备及其吸附胆红素的性能研究[J].功能材料,2010,41(6):1106-1109.XUE M Q,SHI W,ZHANG Q Q.Preparation of new affinity membrane and it used for adsorption of bilirubin[J].Journal of Functional Materials,2010,41(6):1106-1109(in Chinese).
[11]SUN H,ZHANG L,CHAI H,et al.A study of humanγ-globulin adsorption capacity of PVDF hollow fiber affinity membranes containing different amino acid ligands[J].Separation and Purification Technology,2006,48(3):215-222.
[12]WEI Z,HUANG W,LI J,et al.Studies on endotoxin removal mechanism of adsorbents with amino acid ligands[J].Journal of Chromatography B,2007,852(1/2):288-292.
[13]BASAK E,AYDEMIR T.Immobilization of catalase on chitosan and amino acid-modified chitosan beads[J].Artif Cells Nanomed Biotechnol,2013,41(4):269-275.
[14]JU J,HE G,DUAN Z,et al.Improvement of bilirubin adsorption capacity of cellulose acetate/polyethyleneimine membrane using sodium deoxycholate[J].Biochemical Engineering Journal,2013,79:144-152.
[15]MA Z,RAMAKRISHNA S.Electrospun regenerated cellulose nanofiber affinity membrane functionalized with protein A/G for IgG purification[J].Journal of Membrane Science,2008,319(1/2):23-28.
[16]BAYRAMO L G,YALIN E,ARICA M Y.Characterization of polyethylenimine grafted and Cibacron Blue F3GA immobilized poly(hydroxyethylmethacrylate-co-glycydylmethacrylate)membranes and application to bilirubin removal from human serum[J].Colloids and Surfaces A:Physicochemical and Engineering Aspects,2005,264(1):195-202.
[17]ZHANG L,JIN G.Bilirubin removal from human plasma by Cibacron Blue F3GA using immobilized microporous affinity membranous capillary method[J].Journal of Chromatography B,2005,821(1):112-121.
[18]XIA B,ZHANG G L,ZHANG F B.Bilirubin removal by Cibacron Blue F3GA attached nylon-based hydrophilic affinity membrane[J].Journal of Membrane Science,2003,226(1/2):9-20.
[19]SHI W,SHEN Y,JIANG H,et al.Lysine-attached anodic aluminum oxide(AAO)-silica affinity membrane for bilirubin removal[J].Journal of Membrane Science,2010,349(1/2):333-340.
[20]宋晓艳,丁万庆,杨飞,等.PVDF-g-PMMA/POSS纳米纤维膜的制备与表征[J].天津工业大学学报,2015,34(1):12-16.SONG S Y,DING W Q,YANG F,et al.Preparation and characterization of PVDF-g-PMMA/POSS nanofiber membrane[J].Journal of Tianjin Polytechnic University,2015,34(1):12-16(in Chinese).
[21]SHI W,ZHANG F,ZHANG G,et al.Polylysine-immobilized affinity nylon membrane used for bilirubin adsorption[J].Molecular Simulation,2003,29(12):787-790.
[22]SHI W,ZHANG F,ZHANG G.Adsorption of bilirubin with polylysine carrying chitosan-coated nylon affinity membranes[J].Journal of Chromatography B,2005,819(2):301-306.
[23]DENIZLI A,KOCAKULAK M,PISKIN E.Bilirubin removal from human plasma in a packed-bed column system with dyeaffinity microbeads[J].Journal of Chromatography B Biomedical Sciences&Applications,1998,707(1/2):25-31.
[24]WANG W W,ZHANG H,ZHANG Z F,et al.Amine-functionalized PVA-co-PE nanofibrous membrane as affinity membrane with high adsorption capacity for bilirubin[J].Colloids and Surfaces B:Biointerfaces,2017,150:271-278.
[25]MCDONAGH A F,PHIMISTER A,BOIADJIEV S E,et al.Dissociation constants of carboxylic acids by 13C-NMR in DMSO water[J].Tetrahedron Letters,1999,40(49):8515-8518.