高效铜绿微囊藻溶藻菌WJ6的分离鉴定及溶藻特性
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摘要
以铜绿微囊藻为研究对象,从富营养化水体分离了一株高效溶藻菌,通过分析形态学、生理生化特征及16SrDNA序列比对,鉴定了该溶藻菌株,分析了该菌株对铜绿微囊藻的溶藻方式、溶藻特性及其不同培养时期、不同浓度菌液及不同环境因子对溶藻效果的影响,并探讨了其可能的溶藻机制.研究表明:WJ6属于沙雷氏菌属(Serratia sp. GenBank登录号为KY462187);溶藻菌WJ6以胞外释放溶藻物质为主直接溶藻为辅的溶藻方式;溶藻菌WJ6处于对数期时的溶藻率最高达87.50%;菌液浓度达1.4×10~9CFU/mL以上,溶藻率最高为95.69%;在30℃、pH8条件下,溶藻率达90.00%;改良的基本培养基培养的溶藻菌溶藻率最高达98.50%;铵离子浓度大于2mol/L时,溶藻菌的溶藻率98.33%;当盐度为0.5%时,溶藻率较高为92.77%.溶藻菌株WJ6是1株高效溶铜绿微囊藻菌,在富营养化治理方面具有较好的应用前景.
An algicidal bacteria strain with a strong algicidal effect to M.aeruginosa was isolated from the eutrophic water.Then,the algicidal bacteria was identified as WJ6 by analyzing it's morphological features,physiological and biochemical characteristics,as well as phylogenetic analysis of 16 S rDNA sequences.The algae-lysing means of WJ6 and the effects of different culture periods,concentrations of bacterium and environmental factors on the algae-lysing action were investigated to analyze the algicidal characteristics and possible algae-lysing mechanism of the strain WJ6.And the results showed that WJ6 was identified as Serratia sp.(Accession No.KY462187).The algicidal mechanism of WJ6 is indirectively,which disolves the algae mainly by an extracellular products.At logarithmic growth phase,the strain WJ6 can dissolve M.aeruginosa with a removal rate of 87.50%.The bacteria WJ6 with on centration more than 1.4×109 CFU/m L can give the highest removal rate of 95.69%.Under the condition of 30℃ and p H8,the removal rate is 90.00%.The effect of modified basic mediumon the algae-lysing effect of WJ6 is the most obvious with the higests removal rate of 98.50%.Furthermore,the removal rate of the algicidal bacterium is 98.33% when the ammonium ion concentration in the mediumis above 2 mol/L.The removal rate is 92.77% when the salinity is 0.5%.All the results stated above indicate that the algicidal bacteria strain WJ6 have a strong algicidal effect to M.aeruginosa and prospective application in the control of M.aeruginosa in eutrophic water.
An algicidal bacteria strain with a strong algicidal effect to M.aeruginosa was isolated from the eutrophic water.Then,the algicidal bacteria was identified as WJ6 by analyzing it's morphological features,physiological and biochemical characteristics,as well as phylogenetic analysis of 16 S rDNA sequences.The algae-lysing means of WJ6 and the effects of different culture periods,concentrations of bacterium and environmental factors on the algae-lysing action were investigated to analyze the algicidal characteristics and possible algae-lysing mechanism of the strain WJ6.And the results showed that WJ6 was identified as Serratia sp.(Accession No.KY462187).The algicidal mechanism of WJ6 is indirectively,which disolves the algae mainly by an extracellular products.At logarithmic growth phase,the strain WJ6 can dissolve M.aeruginosa with a removal rate of 87.50%.The bacteria WJ6 with on centration more than 1.4×109 CFU/m L can give the highest removal rate of 95.69%.Under the condition of 30℃ and p H8,the removal rate is 90.00%.The effect of modified basic mediumon the algae-lysing effect of WJ6 is the most obvious with the higests removal rate of 98.50%.Furthermore,the removal rate of the algicidal bacterium is 98.33% when the ammonium ion concentration in the mediumis above 2 mol/L.The removal rate is 92.77% when the salinity is 0.5%.All the results stated above indicate that the algicidal bacteria strain WJ6 have a strong algicidal effect to M.aeruginosa and prospective application in the control of M.aeruginosa in eutrophic water.
引文
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[21]张晖,何颖,曹驰骋,等.1株巢湖高效溶藻菌的分离鉴定及其溶藻特性研究[J].环境科学与技术,2018,41(7):1-5.
[22]程新,李昆太,黄林.一株枯草芽孢杆菌的生长特性及抑藻效果研究[J].生物技术通报,2017,33(7):120-125.
[23]张睿,王广军,李志斐,等.枯草芽孢杆菌对铜绿微囊藻抑制效果的研究[J].中国环境科学,2015,35(6):1814-1821.
[24]Su J Q,Yang X R,Zheng T L.Isolation and characterization of a marine algicidal bacterium against the toxic dinoflagellate Alexandrium tamarense[J].Harmful Algae,2007,6(6):799-810.
[25]黄晶晶,汪雪婷,夏赛周,等.溶藻细菌MS7的分类鉴定及其溶藻活性的初步研究[J].海洋与湖沼,2013,44(3):632-637.
[2]李大命,周军,唐晟凯,等.河蟹养殖池塘微囊藻水华毒性及其光合作用活性特征[J].湖泊科学,2015,27(1):113-118.
[3]Alexova R,Fujii M,Birch D.Iron uptake and toxin synthesis in the bloom forming Microcystis aeruginosa under iron limitation[J].Environmental Microbiology,2011,13(4):1064-1077.
[4]张世羊,成水平,贺锋,等.两种不同株铜绿微囊藻培养液对大型溞的毒性效应研究[J].水生生物学报,2008,32(5):637-642.
[5]Imai I,Kimura S.Resistance of the fish-killing dinoflagllate Cochlodinium polykrikoides against algicidal bacteria isolated from the coastal sea of Japan[J].Harmful Algae,2008,7:360-367.
[6]Sherman L A,Jr R M B.Cyanophages and Viruses of Eukaryotic Algae[M].Newly Characterized Protist and Invertebrate Viruses.Springer US,1978:145-234.
[7]Baker K H,Herson D S.Interactions between the diatom Thallasiosira pseudonanna and an associated pseudomonad in a mariculture system[J].Applied&Environmental Microbiology,1978,35(4):791-796.
[8]汪辉,刘玲,牛丹丹,等.一株海洋细菌对中肋骨条藻的溶解效应及其溶藻特性[J].中国环境科学,2011,31(6):971-977.
[9]Proctor L M,Fuhrman J A.Viral mortality of marine bacteria and cyanobacteria[J].Nature,1990,343(6253):60-62.
[10]Imai I,Ishida Y,Hata Y.Killing of marine phytoplankton by a gliding bacterium Cytophaga sp.isolated from the coastal sea of Japan[J].Marine Biology,1993,116(4):527-532.
[11]Banin E,Khare S K,Naider F.Proline-rich peptide from the coral pathogen Vibrio shiloi that inhibits photosynthesis of Zooxanthellae[J].Applied&Environmental Microbiology,2001,67(4):1536-1541.
[12]Furusawa G,Yoshikawa T,Yasuda A.Algicidal activity and gliding motility of Saprospira sp.SS98-5[J].Canadian Journal of Microbiology,2003,49(2):92-100.
[13]Luo J,Wang Y,Tang S.Isolation and Identification of Algicidal Compound from Streptomyces and Algicidal Mechanism to Microcystis aeruginosa[J].Plos One,2013,8(10):e76444.
[14]马宏瑞,章欣,王晓蓉,等.芽孢杆菌Z5溶铜绿微囊藻特性研究[J].中国环境科学,2011,31(5):828-833.
[15]李三华,张奇亚.1株溶藻菌的部分生物学特性及溶鱼腥藻作用[J].环境科学,2013,34(2):583-588.
[16]Li Y,Liu L,Xu Y T,et al.Stress of algicidal substances from a bacterium Exiguo bacterium sp.h10on Microcystis aeruginosa[J].Letters in Applied Microbiology,2017,64(1):57-65.
[17]国家环保总局.水和废水监测分析方法[M].第4版.北京:中国环境科学出版社,2002:670-672.
[18]周瑞,喻融,王梓入,等.溶藻细菌混合培养对铜绿微囊藻溶藻效果的初步研究[J].华中师范大学研究生学报,2005,12(4):138-141.
[19]廖春丽,杨闪闪,许晨,等.一株溶藻细菌NP23的初步分离鉴别及其溶藻作用研究[J].生物技术通报,2012,8:163-167.
[20]黄现恩,谷青,史全良.一株具有藻毒素降解和溶藻功能细菌的分离鉴定[J].环境工程学报,2016,10(7):3919-3924.
[21]张晖,何颖,曹驰骋,等.1株巢湖高效溶藻菌的分离鉴定及其溶藻特性研究[J].环境科学与技术,2018,41(7):1-5.
[22]程新,李昆太,黄林.一株枯草芽孢杆菌的生长特性及抑藻效果研究[J].生物技术通报,2017,33(7):120-125.
[23]张睿,王广军,李志斐,等.枯草芽孢杆菌对铜绿微囊藻抑制效果的研究[J].中国环境科学,2015,35(6):1814-1821.
[24]Su J Q,Yang X R,Zheng T L.Isolation and characterization of a marine algicidal bacterium against the toxic dinoflagellate Alexandrium tamarense[J].Harmful Algae,2007,6(6):799-810.
[25]黄晶晶,汪雪婷,夏赛周,等.溶藻细菌MS7的分类鉴定及其溶藻活性的初步研究[J].海洋与湖沼,2013,44(3):632-637.