游离亚硝酸偶联生物表面活性剂强化污泥厌氧发酵产酸
|
摘要
在中温环境下以剩余污泥为研究对象,在序批式厌氧反应器中探究了游离亚硝酸(FNA)偶联生物表面活性剂鼠李糖脂(RL)对污泥厌氧发酵产酸的影响。实验结果表明:FNA偶联RL能显著提高污泥的水解,当RL的投加量由0.2 g/g提高至0.4 g/g时,溶解性COD(SCOD)的最大含量由451 mg/L提高至512 mg/L,溶解性蛋白质的含量由185 mg/L增加至210 mg/L,进一步提高RL的投加量对SCOD和溶解性蛋白质的增加影响不显著。此外,FNA偶联RL可促进短链脂肪酸(SCFA)的积累,并且当RL的投加量由0增加至0.4 g/g时,SCFA的最大浓度由361 mg/L增加至405 mg/L。FNA偶联RL对SCFA的组分影响不显著,丙酸含量最大,占35%～42%,其次为乙酸,占20%～26%。微生物活性分析表FNA偶联RL能促进水解和酸化酶的活性,而抑制产甲烷酶F420的活性。
The effect of free nitrite(FNA) coupled bio-surfactant rhamnolipid(RL) on acid production from sludge anaerobic fermentation was investigated in a sequencing batch anaerobic reactor at medium temperature with surplus sludge as the study object. The results showed that FNA coupled RL could significantly increase the hydrolysis of sludge, and when the RL mass concentration increased from 0.2 g/g to 0.4 g/g, the maximum soluble COD(SCOD) content increased from 451 mg/L to 512 mg/L, and the soluble protein content increased from 185 mg/L to 210 mg/L, and the further increase of the mass concentration of RL had no significant effect on the increase of SCOD and soluble protein. In addition, FNA coupled RL could promote accumulation of short chain fatty acid(SCFA), and the maximum concentration of SCFA increased from 361 mg/L to 405 mg/L, when the RL concentration increased from 0 to 0.4 g/g. FNA coupled RL had no significant effect on the components of SCFA, and the propionic acid content was the highest, accounting for 35%～42%, followed by acetic acid, accounting for 20%～26%. Microbial activity analysis showed that FNA coupled RL could promote hydrolysis and acid enzyme activity but inhibit activity of the methanogenesis enzyme F420.
The effect of free nitrite(FNA) coupled bio-surfactant rhamnolipid(RL) on acid production from sludge anaerobic fermentation was investigated in a sequencing batch anaerobic reactor at medium temperature with surplus sludge as the study object. The results showed that FNA coupled RL could significantly increase the hydrolysis of sludge, and when the RL mass concentration increased from 0.2 g/g to 0.4 g/g, the maximum soluble COD(SCOD) content increased from 451 mg/L to 512 mg/L, and the soluble protein content increased from 185 mg/L to 210 mg/L, and the further increase of the mass concentration of RL had no significant effect on the increase of SCOD and soluble protein. In addition, FNA coupled RL could promote accumulation of short chain fatty acid(SCFA), and the maximum concentration of SCFA increased from 361 mg/L to 405 mg/L, when the RL concentration increased from 0 to 0.4 g/g. FNA coupled RL had no significant effect on the components of SCFA, and the propionic acid content was the highest, accounting for 35%～42%, followed by acetic acid, accounting for 20%～26%. Microbial activity analysis showed that FNA coupled RL could promote hydrolysis and acid enzyme activity but inhibit activity of the methanogenesis enzyme F420.
引文
[1] Luo J, Chen Y, Feng L. Polycyclic aromatic hydrocarbon affects acetic acid production during anaerobic fermentation of waste activated sludge by altering activity and viability of acetogen[J]. Environmental Science & Technology, 2016, 50(13): 6921-6929.
[2] Cao J, Yu Y, Xie K, et al. Characterizing the free ammonia exposure to the nutrients removal in activated sludge systems[J]. RSC Advances, 2017, 87(7): 55088-55097.
[3] Feng L, Chen Y, Chen X, et al. Anaerobic accumulation of short-chain fatty acids from algae enhanced by damaging cell structure and promoting hydrolase activity[J]. Bioresource Technology, 2018, 250: 777-783.
[4] Feng Y, Zhang Y, Quan X, et al. Enhanced anaerobic digestion of waste activated sludge digestion by the addition of zero valent iron[J]. Water Research, 2014, 52: 242-250.
[5] 王晋, 李习伟, 方新磊,等. 基于氨氮抑制效应的污泥厌氧产酸[J]. 环境工程学报, 2017, 11(5):3091-3098.
[6] de Vrieze J, Smet D, Klok J, et al. Thermophilic sludge digestion improves energy balance and nutrient recovery potential in full-scale municipal wastewater treatment plants[J]. Bioresource Technology, 2016, 218: 1237-1245.
[7] Zahedi S, Icaran P, Yuan Z, et al. Assessment of free nitrous acid pre-treatment on a mixture of primary sludge and waste activated sludge: effect of exposure time and concentration[J]. Bioresource Technology, 2016, 216: 870-875.
[8] Zhao J, Wang D, Li X, et al. Free nitrous acid serving as a pretreatment method for alkaline fermentation to enhance short-chain fatty acid production from waste activated sludge[J]. Water Research, 2015, 78: 111-120.
[9] Li X, Zhao J, Wang D, et al. An efficient and green pretreatment to stimulate short-chain fatty acids production from waste activated sludge anaerobic fermentation using free nitrous acid[J]. Chemosphere, 2016, 144: 160-167.
[10] 易欣, 罗琨, 杨麒,等. 生物表面活性剂强化污泥水解的研究[J]. 环境科学, 2012, 33(9):3202-3207.
[11] Wang L, Liu F, Wang A, et al. Purification, characterization and bioactivity determination of a novel polysaccharide from pumpkin (Cucurbita moschata) seeds[J]. Food Hydrocolloids, 2017, 66: 357-364.
[12] Kolmer E W J E B, Teulen M J A, van den Hombergh E C A, et al. Determination of protein-unbound, active rifampicin in serum by ultrafiltration and ultra performance liquid chromatography with UV detection: a method suitable for standard and high doses of rifampicin[J]. Journal of Chromatography B, 2017, 1063: 42-49.
[13] Zhao J, Gui L, Wang Q, et al. Aged refuse enhances anaerobic digestion of waste activated sludge[J]. Water Research, 2017, 123: 724-733.
[2] Cao J, Yu Y, Xie K, et al. Characterizing the free ammonia exposure to the nutrients removal in activated sludge systems[J]. RSC Advances, 2017, 87(7): 55088-55097.
[3] Feng L, Chen Y, Chen X, et al. Anaerobic accumulation of short-chain fatty acids from algae enhanced by damaging cell structure and promoting hydrolase activity[J]. Bioresource Technology, 2018, 250: 777-783.
[4] Feng Y, Zhang Y, Quan X, et al. Enhanced anaerobic digestion of waste activated sludge digestion by the addition of zero valent iron[J]. Water Research, 2014, 52: 242-250.
[5] 王晋, 李习伟, 方新磊,等. 基于氨氮抑制效应的污泥厌氧产酸[J]. 环境工程学报, 2017, 11(5):3091-3098.
[6] de Vrieze J, Smet D, Klok J, et al. Thermophilic sludge digestion improves energy balance and nutrient recovery potential in full-scale municipal wastewater treatment plants[J]. Bioresource Technology, 2016, 218: 1237-1245.
[7] Zahedi S, Icaran P, Yuan Z, et al. Assessment of free nitrous acid pre-treatment on a mixture of primary sludge and waste activated sludge: effect of exposure time and concentration[J]. Bioresource Technology, 2016, 216: 870-875.
[8] Zhao J, Wang D, Li X, et al. Free nitrous acid serving as a pretreatment method for alkaline fermentation to enhance short-chain fatty acid production from waste activated sludge[J]. Water Research, 2015, 78: 111-120.
[9] Li X, Zhao J, Wang D, et al. An efficient and green pretreatment to stimulate short-chain fatty acids production from waste activated sludge anaerobic fermentation using free nitrous acid[J]. Chemosphere, 2016, 144: 160-167.
[10] 易欣, 罗琨, 杨麒,等. 生物表面活性剂强化污泥水解的研究[J]. 环境科学, 2012, 33(9):3202-3207.
[11] Wang L, Liu F, Wang A, et al. Purification, characterization and bioactivity determination of a novel polysaccharide from pumpkin (Cucurbita moschata) seeds[J]. Food Hydrocolloids, 2017, 66: 357-364.
[12] Kolmer E W J E B, Teulen M J A, van den Hombergh E C A, et al. Determination of protein-unbound, active rifampicin in serum by ultrafiltration and ultra performance liquid chromatography with UV detection: a method suitable for standard and high doses of rifampicin[J]. Journal of Chromatography B, 2017, 1063: 42-49.
[13] Zhao J, Gui L, Wang Q, et al. Aged refuse enhances anaerobic digestion of waste activated sludge[J]. Water Research, 2017, 123: 724-733.