高效液相色谱法测定外科舒筋洗剂中盐酸小檗碱的含量
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摘要
目的建立高效液相色谱法(HPLC)测定外科舒筋剂中盐酸小檗碱含量的方法并予以实施,观察效果。方法配置对照品、供试品及阴性对照品溶液,并采用Agilent Eclipse Plus C18(250.0 mm×4.6 mm, 5μm)色谱柱,以乙腈-0.03 mol/L磷酸二氢钾溶液(28∶72)为流动相,检测波长为347 nm,流速为1.0 ml/min,基于以上条件建立高效液相色谱法进行盐酸小檗碱的含量测定。结果盐酸小檗碱在3.4749～38.2243μg范围内与峰面积线性关系良好,该方法平均回收率为98.06%,相对标准偏差(RSD)为0.93%。结论高效液相色谱法测定外科舒筋剂中盐酸小檗碱含量简便、准确、快速、分离度好,重复性好,干扰小,可用于外科舒筋洗剂的质量控制。
Objective To establish a high performance liquid chromatography(HPLC) method for the determination of berberine hydrochloride in surgical Shujin lotion and to observe its effect. Methods Reference substance, test substance and negative reference substance solution were prepared. A high performance liquid chromatographic method was established for the determination of berberine hydrochloride with Agilent Eclipse Plus C18(250.0 mm × 4.6 mm, 5 μm) column and acetonitrile -0.03 mol/L potassium dihydrogen phosphate solution(28∶72) as mobile phase. The wavelength was 347 nm and the flow rate was 1.0 ml/min. Results Berberine hydrochloride showed a good linear relationship with peak area in the range of 3.4749～38.2243 μg, with average recovery of this method as 98.06% and relative standard deviation(RSD) as 0.93%. Conclusion The determination of berberine hydrochloride in surgical Shujin Lotion by high performance liquid chromatography is simple, accurate, rapid, with good separation, good repeatability and little interference. It can be used for the quality control of surgical Shujin lotion.
Objective To establish a high performance liquid chromatography(HPLC) method for the determination of berberine hydrochloride in surgical Shujin lotion and to observe its effect. Methods Reference substance, test substance and negative reference substance solution were prepared. A high performance liquid chromatographic method was established for the determination of berberine hydrochloride with Agilent Eclipse Plus C18(250.0 mm × 4.6 mm, 5 μm) column and acetonitrile -0.03 mol/L potassium dihydrogen phosphate solution(28∶72) as mobile phase. The wavelength was 347 nm and the flow rate was 1.0 ml/min. Results Berberine hydrochloride showed a good linear relationship with peak area in the range of 3.4749～38.2243 μg, with average recovery of this method as 98.06% and relative standard deviation(RSD) as 0.93%. Conclusion The determination of berberine hydrochloride in surgical Shujin Lotion by high performance liquid chromatography is simple, accurate, rapid, with good separation, good repeatability and little interference. It can be used for the quality control of surgical Shujin lotion.
引文
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[14]丁长玲,张学美,傅勇,等.RP-HPLC法同时测定抗感染药物复方三黄酊中5种成分的含量.药学研究,2017,36(7):386-389.
[15]吴琦,张凡,鞠成国,等.黄柏及其不同炮制品的指纹图谱研究.亚太传统医药,2017,13(20):31-36.
[16]张淹,李士栋,王春艳,等.黄连阿胶胶囊质量标准研究.中药新药与临床药理,2015(6):828-832.
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[18]王绪英,向红,左经会,等.盐酸甲醇正交提取法优选永思小檗中盐酸小檗碱研究.北方园艺,2012(21):95-97.
[19]赵丹庆,刘楠,张力,等.蒙药材通经草中盐酸小檗碱的提取与含量的测定.内蒙古民族大学学报(自然科学版),2013(3):275-276.
[20]赵伟杰.川黄柏中盐酸小檗碱提取新工艺的研究.中国医药导报,2015,12(34):38-42.
[21]刘师师.喜树碱和盐酸小檗碱在离子液体中的溶解性能及应用研究.广西大学,2015.
[2]陈家润,江映珠,吴雪龙.广东省医疗机构制剂质量标准修订存在问题及对策.中国药事,2016,30(3):209-214.
[3]李行诺,翟文丰,周孟宇,等.黄柏化学成分研究.浙江工业大学学报,2012,40(3):244-246.
[4]顾海霞.高效液相色谱法测定柏洁洗剂中盐酸小檗碱含量.中国药业,2008,17(22):40.
[5]任烨,徐辉,葛争艳,等.HPLC法测定降糖消脂片中盐酸小檗碱的含量.中国药房,2015,24(18):2530-2532.
[6]方明余.高效液相色谱法测定复方苦黄洗剂中盐酸小檗碱含量.河北中医,2011,33(12):1857-1859.
[7]杨学芬,陆晓珊.HPLC法测定三九胃泰颗粒中氯化两面针碱的含量.中国民族民间医药,2017,26(13):25-27.
[8]刘丽敏,刘华钢.氯化两面针碱的研究近况.时珍国医国药,2007,18(1):60-62.
[9]李丹丹,江培,杨书美,等.黄柏的化学成分、药理作用及临床应用研究进展.黑龙江医药,2014(3):601-605.
[10]盖晓红,刘素香,任涛,等.黄连的化学成分及药理作用研究进展.中草药,2018,49(20):208-216.
[11]岳琳,李丽,刘颖,等.黄柏炮制后化学成分变化及质量标准研究.四川中医,2017(10):138-141.
[12]潘勇华,蒋红,陈井霞,等.复方黄柏液处方优化及体外经皮渗透效果的研究.药学服务与研究,2017,17(5):366-369.
[13]李学林,王盼盼,刘瑞新,等.3种掩味剂单独或联合应用对黄柏的掩味作用探究及掩味前后化学成分的比较.中国实验方剂学杂志,2017(2):15-19.
[14]丁长玲,张学美,傅勇,等.RP-HPLC法同时测定抗感染药物复方三黄酊中5种成分的含量.药学研究,2017,36(7):386-389.
[15]吴琦,张凡,鞠成国,等.黄柏及其不同炮制品的指纹图谱研究.亚太传统医药,2017,13(20):31-36.
[16]张淹,李士栋,王春艳,等.黄连阿胶胶囊质量标准研究.中药新药与临床药理,2015(6):828-832.
[17]陈良,刘炜,葛朝伦,等.黄金膏中盐酸小檗碱的定性鉴别与含量测定.中国药房,2015(30):4262-4264.
[18]王绪英,向红,左经会,等.盐酸甲醇正交提取法优选永思小檗中盐酸小檗碱研究.北方园艺,2012(21):95-97.
[19]赵丹庆,刘楠,张力,等.蒙药材通经草中盐酸小檗碱的提取与含量的测定.内蒙古民族大学学报(自然科学版),2013(3):275-276.
[20]赵伟杰.川黄柏中盐酸小檗碱提取新工艺的研究.中国医药导报,2015,12(34):38-42.
[21]刘师师.喜树碱和盐酸小檗碱在离子液体中的溶解性能及应用研究.广西大学,2015.