滇牡丹根皮初提物抗菌活性研究
|
摘要
采用乙醇与丙酮分别提取滇牡丹根皮内物质,用13种致病细菌进行活性筛选,采用抑菌圈法评价相应初提物的抗菌活性,并测定具有抗菌活性初提物的最低抑制浓度(MIC),并检测不同温度和不同pH处理后滇牡丹根皮中抗菌物质的抑菌效果。结果表明:乙醇初提物与丙酮初提物能够对供试13种致病细菌中的5种致病细菌具有明显抑菌效果,最低抑制浓度基本相同,只有对无乳链球菌与副溶血弧球菌的MIC值不同;滇牡丹根皮乙醇初提物在不同温度与不同pH处理后,各个处理部分抑菌圈大小没有出现明显变化,因此滇牡丹根皮乙醇初提物有较好的热稳定性和酸碱稳定性;滇牡丹乙醇初提物经过大孔树脂分段纯化后,发现只有100%乙醇洗脱部分具有抗菌活性。
Primary extract were extracted from roots of Paeonia delavayi by ethanol and acetone.And then the activity of the extract was screened by 13 kinds of pathogenic bacteria.Inoculating inhibition zone was used to evaluate the antibacterial activity of primary extract and minimum inhibitory concentration(MIC) were determined.Stabilization of ethanol extracts from roots of P.delavayi was detected by treating with different temperature and different pH.The result showed the ethanol extracts and acetone extracts had inhibitory effect to the 5 pathogenic bacteria of 13 pathogenic bacteria,the MIC of 2 extracts to Bacillus cereus,Straphylococcus haemolyticus,Escherichia coli were similar,and MIC to Streptcococcus agalactiae and Vibrio parahaemolyticus had slight difference.The antibacterial activity of ethanol extracts treated with different temperatures and different pH did not change significantly.It means that stability of the ethanol extracts from roots of P.delavayi was good.After macroporous resin treatment,only 100% ethanol eluted fraction showed antibacterial activity.
Primary extract were extracted from roots of Paeonia delavayi by ethanol and acetone.And then the activity of the extract was screened by 13 kinds of pathogenic bacteria.Inoculating inhibition zone was used to evaluate the antibacterial activity of primary extract and minimum inhibitory concentration(MIC) were determined.Stabilization of ethanol extracts from roots of P.delavayi was detected by treating with different temperature and different pH.The result showed the ethanol extracts and acetone extracts had inhibitory effect to the 5 pathogenic bacteria of 13 pathogenic bacteria,the MIC of 2 extracts to Bacillus cereus,Straphylococcus haemolyticus,Escherichia coli were similar,and MIC to Streptcococcus agalactiae and Vibrio parahaemolyticus had slight difference.The antibacterial activity of ethanol extracts treated with different temperatures and different pH did not change significantly.It means that stability of the ethanol extracts from roots of P.delavayi was good.After macroporous resin treatment,only 100% ethanol eluted fraction showed antibacterial activity.
引文
[1]He D Y,Dai S M.Anti-inflammatory and immunomodulatory effects of Paeonia lactiflora Pall.a traditional Chinese herbal medicine[J].Frontiers in Pharmacology,2011,2(2):10.
[2]洪德元,潘开玉.芍药属牡丹组的分类历史和分类处理[J].植物分类学报,1999,37(4):351-368.
[3]龚洵,潘跃芝,杨志云.滇牡丹的多样性和现状评估[J].西北植物学报,2003,23(2):218-223.
[4]Wu S H,Luo X D,Ma Y B,et al.Monoterpenoid derivatives from Paeonia delavayi[J].Journal of Asian Natural Products Research,2002,4(2):135-140.
[5]吴征镒.云南植物志(第11卷)[M].北京:科学出版社,2000.
[6]李嘉钰.中国牡丹与芍药[M].北京:中国林业出版社,1999.
[7]冯国媚,黄牡丹.中国植物红皮书-稀有濒危植物(第1册)[M].北京:科学出版社,1992.
[8]孔红,成仿云.滇牡丹分类处理的细胞学与分子生物学证据[J].浙江农林大学学报,2010,27(4):601-605.
[9]李奎,郑宝强,王雁,等.野生滇牡丹资源调查与分析[J].中国城市林业,2009,7(6):61-63.
[10]张艳丽,李正红,马宏,等.滇牡丹花色类群性状变异分析[J].植物分类与资源学报,2011,33(2):183-190.
[11]张艳丽,刘秀贤,王雁,等.滇牡丹愈伤组织继代培养中的褐化防治[J].中南林业科技大学学报,2011,31(2):77-81.
[12]周威,张学文,庄以彬,等.波卓霉素生物合成基因簇的异源表达及产量提高[J].华中农业大学学报,2015,34(1):49-58.
[13]李嘉钰.中国牡丹与芍药[M].北京:中国林业出版社,1999.
[14]Liu J K,Yunbao M A,Dagang W U,et al.Paeonilide,a Novel Anti-PAF-active Monoterpenoid-derived Metabolite from[J].Bioscience Biotechnology&Biochemistry,2000,64(7):1511.
[15]Wu S H,Chen Y W,Yang L Y,et al.Monoterpene glycosides from Paeonia delavayi[J].Fitoterapia,2007,78(1):76-78.
[16]王军,闫立昆,李宏铎,等.一株黏细菌Archangium gephyra的鉴定及其代谢产物抑菌活性的研究[J].西北大学学报(自然科学版),2013,43(3):437-441.
[17]赵敏,谭钢文,唐小异,等.十种中药提取物与抗生素合用对铜绿假单胞菌最小抑菌浓度影响的体外实验[J].湖南师范大学学报(医学版),2011,8(4):80-83.
[18]钱慧碧,辛秀兰,兰蓉,等.大孔吸附树脂分离纯化越橘果渣总黄酮的研究[J].吉林农业大学学报,2009,31(5):611-615.
[19]王依健,刘桂军,李修珍.药品流通过程中影响药品稳定性的因素[J].医学信息,2014,27(3):446-447.
[20]李红菊,邓云娟,单天炎,等.醋酸奥曲肽注射液的研发设计:不同p H值对药品稳定性的影响[J].健康必读旬刊,2013,12(3):136.
[21]戴海韵.药品稳定性在药品质量控制中的应用探析[J].科技创新导报,2015(14):229-229.
[22]黎明.牡丹皮药理作用研究概况[J].中国中医药资讯,2010,2(7):222-223.
[23]肖超妮,王培,马翠霞,等.牡丹不同根部位的代谢物分布[J].波普学杂志,2015,32(4):648-660.
[24]胡云飞,徐倩,徐国兵,等.牡丹皮炒制前后UPLC特征指纹图谱比较[J].中国药房,2015,26(6):800-802.
[2]洪德元,潘开玉.芍药属牡丹组的分类历史和分类处理[J].植物分类学报,1999,37(4):351-368.
[3]龚洵,潘跃芝,杨志云.滇牡丹的多样性和现状评估[J].西北植物学报,2003,23(2):218-223.
[4]Wu S H,Luo X D,Ma Y B,et al.Monoterpenoid derivatives from Paeonia delavayi[J].Journal of Asian Natural Products Research,2002,4(2):135-140.
[5]吴征镒.云南植物志(第11卷)[M].北京:科学出版社,2000.
[6]李嘉钰.中国牡丹与芍药[M].北京:中国林业出版社,1999.
[7]冯国媚,黄牡丹.中国植物红皮书-稀有濒危植物(第1册)[M].北京:科学出版社,1992.
[8]孔红,成仿云.滇牡丹分类处理的细胞学与分子生物学证据[J].浙江农林大学学报,2010,27(4):601-605.
[9]李奎,郑宝强,王雁,等.野生滇牡丹资源调查与分析[J].中国城市林业,2009,7(6):61-63.
[10]张艳丽,李正红,马宏,等.滇牡丹花色类群性状变异分析[J].植物分类与资源学报,2011,33(2):183-190.
[11]张艳丽,刘秀贤,王雁,等.滇牡丹愈伤组织继代培养中的褐化防治[J].中南林业科技大学学报,2011,31(2):77-81.
[12]周威,张学文,庄以彬,等.波卓霉素生物合成基因簇的异源表达及产量提高[J].华中农业大学学报,2015,34(1):49-58.
[13]李嘉钰.中国牡丹与芍药[M].北京:中国林业出版社,1999.
[14]Liu J K,Yunbao M A,Dagang W U,et al.Paeonilide,a Novel Anti-PAF-active Monoterpenoid-derived Metabolite from[J].Bioscience Biotechnology&Biochemistry,2000,64(7):1511.
[15]Wu S H,Chen Y W,Yang L Y,et al.Monoterpene glycosides from Paeonia delavayi[J].Fitoterapia,2007,78(1):76-78.
[16]王军,闫立昆,李宏铎,等.一株黏细菌Archangium gephyra的鉴定及其代谢产物抑菌活性的研究[J].西北大学学报(自然科学版),2013,43(3):437-441.
[17]赵敏,谭钢文,唐小异,等.十种中药提取物与抗生素合用对铜绿假单胞菌最小抑菌浓度影响的体外实验[J].湖南师范大学学报(医学版),2011,8(4):80-83.
[18]钱慧碧,辛秀兰,兰蓉,等.大孔吸附树脂分离纯化越橘果渣总黄酮的研究[J].吉林农业大学学报,2009,31(5):611-615.
[19]王依健,刘桂军,李修珍.药品流通过程中影响药品稳定性的因素[J].医学信息,2014,27(3):446-447.
[20]李红菊,邓云娟,单天炎,等.醋酸奥曲肽注射液的研发设计:不同p H值对药品稳定性的影响[J].健康必读旬刊,2013,12(3):136.
[21]戴海韵.药品稳定性在药品质量控制中的应用探析[J].科技创新导报,2015(14):229-229.
[22]黎明.牡丹皮药理作用研究概况[J].中国中医药资讯,2010,2(7):222-223.
[23]肖超妮,王培,马翠霞,等.牡丹不同根部位的代谢物分布[J].波普学杂志,2015,32(4):648-660.
[24]胡云飞,徐倩,徐国兵,等.牡丹皮炒制前后UPLC特征指纹图谱比较[J].中国药房,2015,26(6):800-802.