HPLC分析6种不同花色滇牡丹花瓣中花青素和黄酮
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摘要
滇牡丹是牡丹野生种之一,具有非常丰富的颜色,有白色、黄色、黄绿色、橙色、红色(深红、紫红)、紫色和紫黑色等颜色。采用HPLC和HPLC-DAD-ESI-MS~2同时分析6种不同花色滇牡丹花瓣中花青素和黄酮成分,分析并鉴别出4种花青素化合物和7种黄酮化合物。研究表明:除黄色花瓣,其余5种颜色的花瓣中都含有相同种类的花青素,不同颜色花瓣中花青素的相对百分含量差距比较大。首次从滇牡丹粉色系花瓣中分析并鉴定出矢车菊素双葡萄糖苷(cyanidin-3,5-di-O-glucoside)、矢车菊素单葡萄糖苷(cyanidin-3-O-glucoside)、芍药素双葡萄糖苷(peonidin-3,5-di-O-glucoside)和芍药素单葡萄糖苷(peonidin-3-O-glucoside)。6种颜色滇牡丹花瓣中黄酮化合物的种类几乎相同,仅含量有差异。
Paeonia delavayi is one of the wild species of tree peony, which has rich colors including white,yellow,yellow-green,orange,red( dark-red,purple-red),purple and purple-black. In our study,the anthocyanin and flavonol from 6 kinds of Petals of Paeonia delavayi were analyzed by using high-performance liquid chromatography( HPLC) and high-performance liquid chromatography-diode array detection-mass spectrometry( HPLC-DAD-ESI-MS~2). 4 anthocyanin compounds and 7 flavonol compounds were tentatively identified. The results indicated that excepting the yellow petals,the other 5 kinds of petals have the same anthocyanins and the relative percentage of anthocyanins have significant difference in different colors of petals. Cyanidin-3,5-di-O-glucoside,cyanidin-3-O-glucoside, peonidin-3,5-di-O-glucoside and peonidin-3-O-glucoside were first analyzed in pink Paeonia delavayi. There were no obvious differences in the composition of flavonol among the 6 kinds of petals.
Paeonia delavayi is one of the wild species of tree peony, which has rich colors including white,yellow,yellow-green,orange,red( dark-red,purple-red),purple and purple-black. In our study,the anthocyanin and flavonol from 6 kinds of Petals of Paeonia delavayi were analyzed by using high-performance liquid chromatography( HPLC) and high-performance liquid chromatography-diode array detection-mass spectrometry( HPLC-DAD-ESI-MS~2). 4 anthocyanin compounds and 7 flavonol compounds were tentatively identified. The results indicated that excepting the yellow petals,the other 5 kinds of petals have the same anthocyanins and the relative percentage of anthocyanins have significant difference in different colors of petals. Cyanidin-3,5-di-O-glucoside,cyanidin-3-O-glucoside, peonidin-3,5-di-O-glucoside and peonidin-3-O-glucoside were first analyzed in pink Paeonia delavayi. There were no obvious differences in the composition of flavonol among the 6 kinds of petals.
引文
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[14]刘炎.植物黄酮类化合物功能的研究进展[J].科技信息,2012,39(18):131.
[15]梁倩,郑艳玲,刘云,等.野龙竹竹叶黄酮含量及其抗氧化活性测定实验[J].西部林业科学,2013,42(2):110-112.
[16]Zhang J J,Wang L S,Shu Q Y,et al.Comparison of anthocyanins in non-blotches and blotches of the petals of Xibei tree peony[J].Scientia Horticulturae,2007,114(2):104-111.
[17]Wang X,Cheng C G,Sun Q L,et al.Isolation and purification of four flavonoid constituents from the flower of Paeonia suffruticosa by high-speed counter-current chromatography[J].Journal of Chromatography A,2005,1075(1):127-131.
[2]冯国媚,黄牡丹.中国植物红皮书—稀有濒危植物(第一册)[M].北京:科学出版社,1992.
[3]龚洵,潘跃芝,杨志云.滇牡丹的多样性和现状评估[J].西北植物学报,2003,23(2):218-223.
[4]张艳丽,李正红,马宏,等.滇牡丹花色类群性状变异分析[J].植物分类与资源学报,2011,33(2):183-190.
[5]张艳丽.滇牡丹花色类群遗传背景分析[D].北京:中国林业科学研究院,2011.
[6]李奎,郑宝强,王雁,等.野生滇牡丹资源调查与分析[J].资源保护与利用,2009,7(4):61-63.
[7]刘通,冯丹,陈少瑜,等.4个滇牡丹天然居群遗传多样性的ISSR分析[J].西部林业科学,2014,43(3):31-36.
[8]刘开庆,丁开宇.滇牡丹开花特性的观察[J].安徽农业科学,2008,36(14):5879-5880.
[9]苗翠苹,余莹,陈有为,等.滇牡丹内生真菌的分离及其抑菌活性研究[J].中国药学杂志,2011,46(10):738-741.
[10]周琳,王雁,律春燕,等.云南野生黄牡丹花色素成分的鉴定[J].东北林业大学学报,2011,39(8):52-54.
[11]石碧,杜晓.植物原花色素研究利用进展与发展趋势[J].四川大学学报(工程科学版),2006,38(5):16-24.
[12]姜亚.天然抗氧化剂原花色素的研究趋势[J].广西轻工业,2010,27(10):14-15.
[13]白海娜,王振宇.花色素研究进展[J].中国林副特产,2011,26(5):46-49.
[14]刘炎.植物黄酮类化合物功能的研究进展[J].科技信息,2012,39(18):131.
[15]梁倩,郑艳玲,刘云,等.野龙竹竹叶黄酮含量及其抗氧化活性测定实验[J].西部林业科学,2013,42(2):110-112.
[16]Zhang J J,Wang L S,Shu Q Y,et al.Comparison of anthocyanins in non-blotches and blotches of the petals of Xibei tree peony[J].Scientia Horticulturae,2007,114(2):104-111.
[17]Wang X,Cheng C G,Sun Q L,et al.Isolation and purification of four flavonoid constituents from the flower of Paeonia suffruticosa by high-speed counter-current chromatography[J].Journal of Chromatography A,2005,1075(1):127-131.