基于氧化还原电位的光合细菌补料培养工艺优化
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摘要
为了提高光合细菌培养密度,优化补料工艺,将在线氧化还原电位系统用于光合细菌培养过程中指导主要碳源琥珀酸钠的流加,并且考察琥珀酸钠不同补加浓度以及补料方式对菌体浓度的影响。结果表明,氧化还原电位(ORP)回升可以作为菌体因缺少主要碳源琥珀酸钠而停止增长的信号。相比于批培养,当补入琥珀酸钠速度为0.08 g·(L·h)~(-1)时光合细菌生长达到最佳,细胞密度由0.611 g/L提高至0.927 g/L。而基于ORP指导的间歇碳源流加工艺优于连续流加,最终菌群培养密度可提高至1.46 g/L。基于氧化还原电位的光合细菌补料培养工艺大大提高了生产效益,为后续的工业化生产提供了一定的指导意义。
In order to improve the culture density of photosynthetic bacteria and optimize the feeding process,the online redox potential system was used to guide the flow of the main carbon source sodium succinate during the photosynthetic bacteria culture process. The different supplemental concentrations of sodium succinate and the feeding mode were investigated. The results showed that oxidation reduetion potential(ORP)rebound could be used as a signal that the bacteria stopped growing due to the lack of the main carbon source sodium succinate. Compared with batch culture,photosynthetic bacteria grew optimally when the rate of sodium succinate was 0.08 g·(L·h)~(-1),and the cell density increased from 0.611 g/L to 0.927 g/L. The intermittent carbon source addition process based on ORP was superior to continuous flow addition,and the final microbial culture density could increased to 1.46 g/L. The feeding culture process of photosynthetic bacteria based on ORP greatly improved the production efficiency and provided certain guiding significance for the subsequent industrial production.
In order to improve the culture density of photosynthetic bacteria and optimize the feeding process,the online redox potential system was used to guide the flow of the main carbon source sodium succinate during the photosynthetic bacteria culture process. The different supplemental concentrations of sodium succinate and the feeding mode were investigated. The results showed that oxidation reduetion potential(ORP)rebound could be used as a signal that the bacteria stopped growing due to the lack of the main carbon source sodium succinate. Compared with batch culture,photosynthetic bacteria grew optimally when the rate of sodium succinate was 0.08 g·(L·h)~(-1),and the cell density increased from 0.611 g/L to 0.927 g/L. The intermittent carbon source addition process based on ORP was superior to continuous flow addition,and the final microbial culture density could increased to 1.46 g/L. The feeding culture process of photosynthetic bacteria based on ORP greatly improved the production efficiency and provided certain guiding significance for the subsequent industrial production.
引文
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[2]Zeiger L,Grammel H.Model-based high cell density cultivation of Rhodospirillum rubrum under respiratory dark conditions[J].Biotechnology & Bioengineering,2010,105(4):729-739.
[3]黄雪娇,杨冲,罗雅雪,等.光合细菌在水污染治理中的研究进展[J].中国生物工程杂志,2014,34(11):119-124.
[4]王占诚.LED光源在光合细菌培养中的应用[D].上海:华东师范大学,2017.
[5]王国惠.污染控制中混合菌的研究[J].环境技术,2004(4):39-42.
[6]王泽建,王萍,张琴,等.发酵过程生理参数检测传感器技术与过程优化[J].生物产业技术,2018(1):19-32.
[7]Lin Yan Huin,Hwang Sz-chwun John,Jian Tong Gong,et al.Using redox potential to detect microbial activities during clavulanic acid biosynthesis in Streptomyces clavuligerus[J].Biotechnology Letters,2005,27:1791-1795.
[8]姜岷,李建,陈可泉,等.氧化还原电位调控Actinobacillus succinogenes厌氧发酵产丁二酸的影响[J].化工进展,2008,27(8):1250-126.
[9]Xin Z G,Jiang H L,Hyun-dong Shin,et al.Improved propionic acid production with metabolically engineered Propionibacterium jensenii by an oxidoreduction potential-shift control strategy[J].Bioresource Technology,2015(175):606-612.
[10]田锡炜.基于过程氧代谢和渗透压应激响应分析的乳酸发酵优化[D].上海:华东理工大学,2015.
[11]Sato K,Aoki M,Noyori R.A green route to adipic acid:Direct oxidation cyclohexenes with 30 percent hydrogen peroxide[J].Science,1998,281:1646-1647.
[12]全亚玲,戴静,马力,等.沼泽红假单胞菌培养基配方及培养条件优化实验研究[J].西南民族大学学报:自然科学版,2012,38(3):383-386.
[13]李旭.光合细菌(Rhodobacter sphaeroides)生物制氢及其光生物反应器研究[D].上海:华东理工大学,2011.
[14]舒威.底物波动环境下黑曲霉中心碳代谢动态响应规律初步探究[D].上海:华东理工大学,2017.
[15]谢学旺.光合细菌产氢过程中能质传输及超声强化特性研究[D].重庆:重庆大学,2011.