乳酸菌的热胁迫研究进展
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摘要
乳酸菌和其他自由生活的微生物一样,在发酵及食品加工、贮藏过程中经常暴露于各种环境胁迫条件下,包括饥饿胁迫、渗透压胁迫以及热胁迫等,增加胁迫抗性是提高生物量及代谢产物积累的有效策略。热胁迫可能是自然界及工业应用中微生物所面临的最常见压力。目前研究表明来自不同生境的乳酸菌通过基因表达的快速变化对温度的突然升高做出反应,从而导致热休克蛋白的蛋白质水平升高。热休克蛋白在进化过程中具有高度保守性,在正常条件下,其有助于受体调节,细胞骨架稳定及蛋白质的折叠、组装、运输、降解。在热胁迫条件下,其功能变得尤为重要。综述了乳酸菌的热胁迫耐受机制及不同乳酸菌的热胁迫反应,为研究乳酸菌热胁迫应答机制提供一定的理论借鉴,有利于乳酸菌的工业化应用。
Lactic acid bacteria(LAB),like other free-living microorganisms,are usually exposed to various environmental stress conditions in the course of microbial fermentation,food processing and storage,which include starvation stress,osmotic stress,and heat stress.Enhancement of stress resistance is considered an effective strategy for better cell growth and increased the yield of metabolites.Heat stress is probably the most common stress,which bacteria and other organisms are confronted in the natural world and industrial applications.Some studies had demonstrated that LAB from different habitats responded to a sudden increase in temperature by rapid changes in gene expression resulting in elevated levels of heat-shock proteins(HSPs).Under normal conditions,HSPs assist in receptor regulation,cytoskeleton stabilization,and protein folding,assembly,transport,degradation,and under heat stress these functions become especially important.In this paper,the tolerance mechanism of heat stress in LAB and heat stress reaction of different LAB were discussed in order to provide a theoretical reference for the study of the heat stress response mechanism in LAB and to facilitate the industrial application of LAB.
Lactic acid bacteria(LAB),like other free-living microorganisms,are usually exposed to various environmental stress conditions in the course of microbial fermentation,food processing and storage,which include starvation stress,osmotic stress,and heat stress.Enhancement of stress resistance is considered an effective strategy for better cell growth and increased the yield of metabolites.Heat stress is probably the most common stress,which bacteria and other organisms are confronted in the natural world and industrial applications.Some studies had demonstrated that LAB from different habitats responded to a sudden increase in temperature by rapid changes in gene expression resulting in elevated levels of heat-shock proteins(HSPs).Under normal conditions,HSPs assist in receptor regulation,cytoskeleton stabilization,and protein folding,assembly,transport,degradation,and under heat stress these functions become especially important.In this paper,the tolerance mechanism of heat stress in LAB and heat stress reaction of different LAB were discussed in order to provide a theoretical reference for the study of the heat stress response mechanism in LAB and to facilitate the industrial application of LAB.
引文
[1]Valeriano V,Parungao-balolong M,Kang D K.In vitro evaluation of the mucin-adhesion ability and probiotic potential of Lactobacillus mucosae LM1[J].Journal of Applied Microbiology,2014,117:485-497
[2]Liu B B,Fu N,Woo M W,et al.Heat stability of Lactobacillus rhamnosus GG and its cellular membrane during droplet drying and heat treatment[J].Food Research International,2018,112:56-65
[3]Cebrián G,Condón S,Mańas P.Heat resistance,membrane fluidity and sublethal damage in Staphylococcus aureus cells grown at different temperatures[J].International Journal of Food Microbiology,2018,289(16):49-56
[4]Garbuz D G,Evgen’ev M B.The evolution of heat shock genes and expression patterns of heat shock proteins in the species from temperature contrasting habitats[J].Russian Journal of Genetics,2017,53(1):21-38
[5]Hickisch A,Beer R,Vogel R F,et al.Influence of lupin-based milk alternative heat treatment and exopolysaccharideproducing lactic acid bacteria on the physical characteristics of lupin-based yogurt alternatives[J].Food Research International,2016,84:180-188
[6]Marklund E G,Zhang Y,Basha E,et al.Structural and functional aspects of the interaction partners of the small heat-shock protein in Synechocystis[J].Cell Stress and Chaperones,2018,23(4):723-732
[7]Kayumov A R,Bogachev M I,Manuvera V A,et al.Recombinant small heat shock protein from Acholeplasma laidlawii increases the Escherichia coli viability in thermal stress by selective protein rescue[J].Molecular Biology,2017,51(1):112-121
[8]Garbuz D G.Regulation of heat shock gene expression in response to stress[J].Molecular Biology,2017,51(3):352-367
[9]Koch B,Kilstrup M,Vogensen F K,et al.Heterogeneous expression of Dna K gene from Alicyclobacillus acidoterrestris improves the resistance of Escherichia coli against heat and acid stress[J].AMB Express,2017,7(1):1-7
[10]Rossi C C,Oliveira L L D,Rodrigues D D C,et al.Expression of the stress-response regulators Cts R and Hrc Ain the uropathogen Staphylococcus saprophyticus during heat shock[J].Antonie Van Leeuwenhoek,2017,110(8):1105-1111
[11]Suokko A,Poutanen M,Savijoki K,et al.Clp L is essential for induction of thermotolerance and is potentially part of the Hrc A regulon in Lactobacillus gasseri[J].Proteomics,2008,8(5):1029-1041
[12]Kang K,Lee J S,Yoo M,et al.The influence of Htr Aexpression on the growth of Streptococcus mutans during acid stress[J].Molecules and Cells,2010,29(3):297-304
[13]Harrer A,Boehm M,Backert S,et al.Overexpression of serine protease Htr A enhances disruption of adherens junctions,paracellular transmigration and type IV secretion of CagA by Helicobacter pylori[J].Gut Pathogens,2017,9:40
[14]Ito K,Akiyama Y.Cellular functions,mechanism of action,and regulation of Fts H protease[J].Annual Review of Microbiology,2005,59(59):211-231
[15]Fiocco D,Collins M,Muscariello L,et al.The Lactobacillus plantarum fts H gene is a novel member of the Cts R stress response regulon[J].Journal of Bacteriology,2009,191(5):1688-1694
[16]Wang Q,Ji F,Guo J,et al.Lot S/Lot R/Clp,a novel signal pathway responding to temperature,modulating protease expression via c-di-GMP mediated manner in Stenotrophomonas maltophilia FF11[J].Microbiological Research,2018,5:60-73
[17]Roy S S,Patra M,Dasgupta R,et al.Mutual interaction study between Dna K-Gro EL-Ft SH with heat shock regulatorσ32to explain prokaryotic heat shock regulation[J].Information Systems Design and Intelligent Applications,2015,340:55-61
[18]Boniface A,Parquet C,Arthur M,et al.Effect of inactivation of stress response regulators on the growth and survival of Streptococcus thermophilus Sfi39[J].International Journal of Food Microbiology,2009,129(3):211-220
[19]Fiocco D,Capozzi V,Collins M,et al.Characterization of the Cts R stress response regulon in Lactobacillus plantarum[J].Journal of Bacteriology,2010,192(3):896-900
[20]Guchte M V D,Penaud S,Grimaldi C,et al.The complete genome sequence of Lactobacillus bulgaricus reveals extensive and ongoing reductive evolution[J].Proceedings of the National Academy of Sciences of the United States of America,2006,103(24):9274-9279
[21]赵亚荣,张文羿,孙天松.乳酸菌环境胁迫应答分子机制研究进展[J].中国乳品工业,2014,42(4):42-45ZHAO Ya-rong,ZHANG Wen-yi,SUN Tian-song.Research on the molecular mechanisms of lactic acid bacteria responding to environmental stress[J].Dairy Industry,2014,42(4):42-45
[22]Schumann W.Regulation of the heat shock response in bacteria[J].Prokaryotic Chaperonins,2017,11:21-36
[23]陈霞,杨振泉,黄玉军,等.乳酸菌环境胁迫应激的分子调控机制研究进展[J].中国乳品工业,2011,39(1):34-37CHEN Xia,YANG Zhen-quan,HUANG Yu-jun,et al.Research on the molecular mechanism of lactic acid bacteria’s responses to environmental stress[J].Dairy Industry,2011,39(1):34-37
[24]Skinner M M,Trempy J E.Expression of clp X,an ATPase subunit of the Clp protease,is heat and cold shock inducible in Lactococcus lactis[J].Journal of Dairy Science,2001,84(8):1783-1785
[25]Kang C H,Jeon H,Shin Y J,et al.Heat adaptation improves viability of Lactococcus lactis subsp.lactis HE-1 after heat stress[J].Food Science and Biochnology,2015,24(5):1823-1827
[26]Rallu F,Gruss A,Ehrlich S D,et al.Short communication:Salt tolerance of Lactococcus lactis R-604 as influenced by mild stresses from ethanol,heat,hydrogen peroxide,and UVlight[J].Journal of Dairy Science,2017,100(6):4290-4293
[27]Gouesbert G,Jan G,Boyaval P.S-layer production by Lactobacillus acidophilus IBB 801 under environmental stress conditions[J].Applied Microbiology and Biotechnology,2016,100(1):4573-4583
[28]Wang H,Zhang Y,Xi Q,et al.Heat acclimation of bifidobacterium longum and proteomic changes behind it[J].Probiotics and Antimicrobial Proteins,2017,9(3):255-261
[29]陈旭娇,丁缪华,顾瑞霞,等.热应激对鼠李糖乳杆菌grx19生长及耐热特性的影响研究[J].食品科技,2013,38(8):2-5CHEN Xun-jiao,DING Niu-hua,GU Rui-xia,et al.The influence of heat stress on the growth and thermoduric properties of Lactobacillus Rhamnosus GRX19[J].Food Science and Technology,2013,38(8):2-5
[30]Suo Y,Luo S,Zhang Y,et al.Enhanced butyric acid tolerance and production by class I heat shock protein-overproducing Clostridium tyrobutyricum ATCC25755[J].Journal of Industrial Microbiology&Biotechnology,2017,44(8):1145-1156
[31]Sottile M L,Nadin S B.Heat shock proteins and DNA repair mechanisms:an updated overview[J].Cell Stress and Chaperones,2018,23(3):303-315
[32]Suokko A,Poutanen M,Savijoki K,et al.Clp L is essential for induction of thermotolerance and is potentially part of the Hrc A regulon in Lactobacillus gasseri[J].Proteomics,2008,8(5):1029-1041
[33]王学良,韩雪,王海娟,等.乳酸菌在各种胁迫下的应激反应研究进展[J].食品工业科技,2015,36(6):365-368WAMG Xue-liang,HAN Xue,WANG Hai-juan,et al.Studying progress of Lactobacillus’s responses in a variety of stress[J].Science and Technology of Food Industry,2015,36(6):365-368
[34]Lim E M,Ehrlich S D,Maguin E.Biophysical characterization of the Lactobacillus delbrueckii subsp.bulgaricus membrane during cold and osmotic stress and its relevance for cryopreservation[J].Applied Microbiology and Biotechnology,2017,101(4):1427-1441
[35]Nefedova V V,Muranova L K,Sudnitsyna M V,et al.Small heat shock proteins and distal hereditary neuropathies[J].Biochemistry,2015,80(13):1734-1747
[36]Oz E,Kaban G.The effect of autochthonous Lactobacillus plantarum on volatile compounds in heat-treated sucuk[J].Journal of Biotechnology,2018,280:S60
[37]Kulkarni S,Haq S F,Samant S,et al.Adaptation of Lactobacillus acidophilus to thermal stress yields a thermotolerant variant which also virulence determinant exhibits improved survival at p H 2[J].Probiotics and Antimicrobial Proteins,2017,8:1-11
[38]Schumann W.Regulation of bacterial heat shock stimulons[J].Cell Stress and Chaperones,2016,21(6):959-968
[39]Huang S,Cheng X D.Significant effect of Ca2+on improving the heat resistance of lactic acid bacteria[J].FEMSMicrobiology Letters,2013,344(1):31-38
[40]Haddaji N,Mahdhi A K,Ismaiil M B,Bakhrouf A.Effect of environmental stress on cell surface and membrane fatty acids of Lactobacillus plantarum[J].Archives of Microbiology,2017,199(9):1243-1250
[41]张筠,孟祥晨.乳酸菌的胁迫应答及其对碳水化合物代谢的影响[J].中国食品学报,2017,17(6):145-149ZNANG Jun,MENG Xiang-chen.Stress responses and impact of carbonhydrate metabolism in lactic acid bacteria[J].Journal of Chinese Institute of Food Science and Technology,2017,17(6):145-149
[42]Varcamonti M,Graziano M R,Pezzopane R,et al.Impaired temperature stress response of a streptococcus thermophilus deo D mutant[J].Applied and Environmental Microbiology,2003,69(2):1287-1289
[43]Varcamonti M,Arsenijevic S,Martirani L,et al.Expression of the heat shock gene clp L of streptococcus thermophilus is induced by both heat and cold shock[J].Microbial Cell Factories,2006,5(1):1-6
[44]Krab I M,Te Biesebeke R,Bernardi A,et al.Elongation factor Ts can act as a steric chaperone by increasing the solubility of nucleotide binding-impaired elongation factor-Tu[J].Biochemistry,2001,40(29):8531-8535
[45]Giliberti G,Naclerio G,Martirani L,et al.Alteration of cell morphology and viability in a rec A mutant of streptococcus thermophilus upon induction of heat shock and nutrient starvation[J].Gene,2002,295(1):1-6
[46]Giliberti G,Baccigalupi L,Cordone A,et al.Transcriptional analysis of the recA gene of streptococcus thermophilus[J].Microbial Cell Factories,2006,5(1):29
[47]FoulquiéMoreno M R,Sarantinopoulos P,Tsakalidou E,et al.The role and application of enterococci in food and health[J].International Journal of Food Microbiology,2006,106(1):1-24
[48]RincéA,Flahaut S,Auffray Y.Identification of general stress genes in Enterococcus faecalis[J].International Journal of Food Microbiology,2000,55(1-3):87-91
[49]Giard J C,Laplace J,RincéA,et al.The stress proteome of Enterococcus faecalis[J].Electrophoresis,2001,22(14):2947-2949
[50]Benachour A,Muller C,Dabrowski-Coton M,et al.The Enterococcus faecalis Sig V protein is an extracytoplasmic function sigma factor contributing to survival following heat,acid,and ethanol treatments[J].Journal of Bacteriology,2005,187(3):1022-1035
[51]Mills S,Stanton C,Fitzgerald G F,et al.Enhancing the stress responses of probiotics for a lifestyle from gut to product and back again[J].Microbial Cell Factories,2011,10:S19
[52]Vernazza C L,Gibson G R,Rastall R A.Carbohydrate preference,acid tolerance and bile tolerance in five strains of Bifidobacterium[J].Journal of Applied Microbiology,2006,100(4):846-853
[53]Khaskheli G B,Zuo F L,Yu R,et al.Overexpression of small heat shock protein enhances heat-and salt-stress tolerance of Bifidobacterium longum NCC2705[J].Current Microbiology,2015,71(1):8-15
[54]Ventura M,Kenny J G,Zhang Z,et al.The clp B gene of Bifidobacterium breve UCC 2003:transcriptional analysis and first insights into stress induction[J].Microbiology,2005,151(9):2861-2872
[55]Zomer A,Fernandez M,Kearney B,et al.An interactive regulatory network controls stress response in Bifidobacterium breve UCC2003[J].Journal of Bacteriology,2009,191(2):7039-7049
[56]Nakamoto H,Vigh L.The small heat shock proteins and their clients[J].Cellular and Molecular Life Sciences,2007,64(3):294-306
[57]Guan N,Li J,Shin H,et al.Microbial response to environmental stresses:from fundamental mechanisms to practical applications[J].Applied Microbiology and Biotechnology,2017,101(10):3991-4008
[58]Coucheney F,Desroche N,Bou M,et al.A new approach for selection of Oenococcus oeni strains in order to produce malolactic starter[J].International Journal of Food Microbiology,2005,105(3):463-470
[59]Coucheney F,Gal L,Beney L,et al.A small HSP,Lo18interacts with the cell membrane and modulates lipid physical state under heat shock conditions in a lactic acid bacterium[J].Biochimica Et Biophysica Acta,2005,1720(1):92-98
[60]Li Y,Xu X,Qu R,et al.Heterologous expression of Oenococcus oeni s HSP20 confers temperature stress tolerance in Escherichia coli[J].Cell Stress and Chaperones,2018,23(4):653-662
[2]Liu B B,Fu N,Woo M W,et al.Heat stability of Lactobacillus rhamnosus GG and its cellular membrane during droplet drying and heat treatment[J].Food Research International,2018,112:56-65
[3]Cebrián G,Condón S,Mańas P.Heat resistance,membrane fluidity and sublethal damage in Staphylococcus aureus cells grown at different temperatures[J].International Journal of Food Microbiology,2018,289(16):49-56
[4]Garbuz D G,Evgen’ev M B.The evolution of heat shock genes and expression patterns of heat shock proteins in the species from temperature contrasting habitats[J].Russian Journal of Genetics,2017,53(1):21-38
[5]Hickisch A,Beer R,Vogel R F,et al.Influence of lupin-based milk alternative heat treatment and exopolysaccharideproducing lactic acid bacteria on the physical characteristics of lupin-based yogurt alternatives[J].Food Research International,2016,84:180-188
[6]Marklund E G,Zhang Y,Basha E,et al.Structural and functional aspects of the interaction partners of the small heat-shock protein in Synechocystis[J].Cell Stress and Chaperones,2018,23(4):723-732
[7]Kayumov A R,Bogachev M I,Manuvera V A,et al.Recombinant small heat shock protein from Acholeplasma laidlawii increases the Escherichia coli viability in thermal stress by selective protein rescue[J].Molecular Biology,2017,51(1):112-121
[8]Garbuz D G.Regulation of heat shock gene expression in response to stress[J].Molecular Biology,2017,51(3):352-367
[9]Koch B,Kilstrup M,Vogensen F K,et al.Heterogeneous expression of Dna K gene from Alicyclobacillus acidoterrestris improves the resistance of Escherichia coli against heat and acid stress[J].AMB Express,2017,7(1):1-7
[10]Rossi C C,Oliveira L L D,Rodrigues D D C,et al.Expression of the stress-response regulators Cts R and Hrc Ain the uropathogen Staphylococcus saprophyticus during heat shock[J].Antonie Van Leeuwenhoek,2017,110(8):1105-1111
[11]Suokko A,Poutanen M,Savijoki K,et al.Clp L is essential for induction of thermotolerance and is potentially part of the Hrc A regulon in Lactobacillus gasseri[J].Proteomics,2008,8(5):1029-1041
[12]Kang K,Lee J S,Yoo M,et al.The influence of Htr Aexpression on the growth of Streptococcus mutans during acid stress[J].Molecules and Cells,2010,29(3):297-304
[13]Harrer A,Boehm M,Backert S,et al.Overexpression of serine protease Htr A enhances disruption of adherens junctions,paracellular transmigration and type IV secretion of CagA by Helicobacter pylori[J].Gut Pathogens,2017,9:40
[14]Ito K,Akiyama Y.Cellular functions,mechanism of action,and regulation of Fts H protease[J].Annual Review of Microbiology,2005,59(59):211-231
[15]Fiocco D,Collins M,Muscariello L,et al.The Lactobacillus plantarum fts H gene is a novel member of the Cts R stress response regulon[J].Journal of Bacteriology,2009,191(5):1688-1694
[16]Wang Q,Ji F,Guo J,et al.Lot S/Lot R/Clp,a novel signal pathway responding to temperature,modulating protease expression via c-di-GMP mediated manner in Stenotrophomonas maltophilia FF11[J].Microbiological Research,2018,5:60-73
[17]Roy S S,Patra M,Dasgupta R,et al.Mutual interaction study between Dna K-Gro EL-Ft SH with heat shock regulatorσ32to explain prokaryotic heat shock regulation[J].Information Systems Design and Intelligent Applications,2015,340:55-61
[18]Boniface A,Parquet C,Arthur M,et al.Effect of inactivation of stress response regulators on the growth and survival of Streptococcus thermophilus Sfi39[J].International Journal of Food Microbiology,2009,129(3):211-220
[19]Fiocco D,Capozzi V,Collins M,et al.Characterization of the Cts R stress response regulon in Lactobacillus plantarum[J].Journal of Bacteriology,2010,192(3):896-900
[20]Guchte M V D,Penaud S,Grimaldi C,et al.The complete genome sequence of Lactobacillus bulgaricus reveals extensive and ongoing reductive evolution[J].Proceedings of the National Academy of Sciences of the United States of America,2006,103(24):9274-9279
[21]赵亚荣,张文羿,孙天松.乳酸菌环境胁迫应答分子机制研究进展[J].中国乳品工业,2014,42(4):42-45ZHAO Ya-rong,ZHANG Wen-yi,SUN Tian-song.Research on the molecular mechanisms of lactic acid bacteria responding to environmental stress[J].Dairy Industry,2014,42(4):42-45
[22]Schumann W.Regulation of the heat shock response in bacteria[J].Prokaryotic Chaperonins,2017,11:21-36
[23]陈霞,杨振泉,黄玉军,等.乳酸菌环境胁迫应激的分子调控机制研究进展[J].中国乳品工业,2011,39(1):34-37CHEN Xia,YANG Zhen-quan,HUANG Yu-jun,et al.Research on the molecular mechanism of lactic acid bacteria’s responses to environmental stress[J].Dairy Industry,2011,39(1):34-37
[24]Skinner M M,Trempy J E.Expression of clp X,an ATPase subunit of the Clp protease,is heat and cold shock inducible in Lactococcus lactis[J].Journal of Dairy Science,2001,84(8):1783-1785
[25]Kang C H,Jeon H,Shin Y J,et al.Heat adaptation improves viability of Lactococcus lactis subsp.lactis HE-1 after heat stress[J].Food Science and Biochnology,2015,24(5):1823-1827
[26]Rallu F,Gruss A,Ehrlich S D,et al.Short communication:Salt tolerance of Lactococcus lactis R-604 as influenced by mild stresses from ethanol,heat,hydrogen peroxide,and UVlight[J].Journal of Dairy Science,2017,100(6):4290-4293
[27]Gouesbert G,Jan G,Boyaval P.S-layer production by Lactobacillus acidophilus IBB 801 under environmental stress conditions[J].Applied Microbiology and Biotechnology,2016,100(1):4573-4583
[28]Wang H,Zhang Y,Xi Q,et al.Heat acclimation of bifidobacterium longum and proteomic changes behind it[J].Probiotics and Antimicrobial Proteins,2017,9(3):255-261
[29]陈旭娇,丁缪华,顾瑞霞,等.热应激对鼠李糖乳杆菌grx19生长及耐热特性的影响研究[J].食品科技,2013,38(8):2-5CHEN Xun-jiao,DING Niu-hua,GU Rui-xia,et al.The influence of heat stress on the growth and thermoduric properties of Lactobacillus Rhamnosus GRX19[J].Food Science and Technology,2013,38(8):2-5
[30]Suo Y,Luo S,Zhang Y,et al.Enhanced butyric acid tolerance and production by class I heat shock protein-overproducing Clostridium tyrobutyricum ATCC25755[J].Journal of Industrial Microbiology&Biotechnology,2017,44(8):1145-1156
[31]Sottile M L,Nadin S B.Heat shock proteins and DNA repair mechanisms:an updated overview[J].Cell Stress and Chaperones,2018,23(3):303-315
[32]Suokko A,Poutanen M,Savijoki K,et al.Clp L is essential for induction of thermotolerance and is potentially part of the Hrc A regulon in Lactobacillus gasseri[J].Proteomics,2008,8(5):1029-1041
[33]王学良,韩雪,王海娟,等.乳酸菌在各种胁迫下的应激反应研究进展[J].食品工业科技,2015,36(6):365-368WAMG Xue-liang,HAN Xue,WANG Hai-juan,et al.Studying progress of Lactobacillus’s responses in a variety of stress[J].Science and Technology of Food Industry,2015,36(6):365-368
[34]Lim E M,Ehrlich S D,Maguin E.Biophysical characterization of the Lactobacillus delbrueckii subsp.bulgaricus membrane during cold and osmotic stress and its relevance for cryopreservation[J].Applied Microbiology and Biotechnology,2017,101(4):1427-1441
[35]Nefedova V V,Muranova L K,Sudnitsyna M V,et al.Small heat shock proteins and distal hereditary neuropathies[J].Biochemistry,2015,80(13):1734-1747
[36]Oz E,Kaban G.The effect of autochthonous Lactobacillus plantarum on volatile compounds in heat-treated sucuk[J].Journal of Biotechnology,2018,280:S60
[37]Kulkarni S,Haq S F,Samant S,et al.Adaptation of Lactobacillus acidophilus to thermal stress yields a thermotolerant variant which also virulence determinant exhibits improved survival at p H 2[J].Probiotics and Antimicrobial Proteins,2017,8:1-11
[38]Schumann W.Regulation of bacterial heat shock stimulons[J].Cell Stress and Chaperones,2016,21(6):959-968
[39]Huang S,Cheng X D.Significant effect of Ca2+on improving the heat resistance of lactic acid bacteria[J].FEMSMicrobiology Letters,2013,344(1):31-38
[40]Haddaji N,Mahdhi A K,Ismaiil M B,Bakhrouf A.Effect of environmental stress on cell surface and membrane fatty acids of Lactobacillus plantarum[J].Archives of Microbiology,2017,199(9):1243-1250
[41]张筠,孟祥晨.乳酸菌的胁迫应答及其对碳水化合物代谢的影响[J].中国食品学报,2017,17(6):145-149ZNANG Jun,MENG Xiang-chen.Stress responses and impact of carbonhydrate metabolism in lactic acid bacteria[J].Journal of Chinese Institute of Food Science and Technology,2017,17(6):145-149
[42]Varcamonti M,Graziano M R,Pezzopane R,et al.Impaired temperature stress response of a streptococcus thermophilus deo D mutant[J].Applied and Environmental Microbiology,2003,69(2):1287-1289
[43]Varcamonti M,Arsenijevic S,Martirani L,et al.Expression of the heat shock gene clp L of streptococcus thermophilus is induced by both heat and cold shock[J].Microbial Cell Factories,2006,5(1):1-6
[44]Krab I M,Te Biesebeke R,Bernardi A,et al.Elongation factor Ts can act as a steric chaperone by increasing the solubility of nucleotide binding-impaired elongation factor-Tu[J].Biochemistry,2001,40(29):8531-8535
[45]Giliberti G,Naclerio G,Martirani L,et al.Alteration of cell morphology and viability in a rec A mutant of streptococcus thermophilus upon induction of heat shock and nutrient starvation[J].Gene,2002,295(1):1-6
[46]Giliberti G,Baccigalupi L,Cordone A,et al.Transcriptional analysis of the recA gene of streptococcus thermophilus[J].Microbial Cell Factories,2006,5(1):29
[47]FoulquiéMoreno M R,Sarantinopoulos P,Tsakalidou E,et al.The role and application of enterococci in food and health[J].International Journal of Food Microbiology,2006,106(1):1-24
[48]RincéA,Flahaut S,Auffray Y.Identification of general stress genes in Enterococcus faecalis[J].International Journal of Food Microbiology,2000,55(1-3):87-91
[49]Giard J C,Laplace J,RincéA,et al.The stress proteome of Enterococcus faecalis[J].Electrophoresis,2001,22(14):2947-2949
[50]Benachour A,Muller C,Dabrowski-Coton M,et al.The Enterococcus faecalis Sig V protein is an extracytoplasmic function sigma factor contributing to survival following heat,acid,and ethanol treatments[J].Journal of Bacteriology,2005,187(3):1022-1035
[51]Mills S,Stanton C,Fitzgerald G F,et al.Enhancing the stress responses of probiotics for a lifestyle from gut to product and back again[J].Microbial Cell Factories,2011,10:S19
[52]Vernazza C L,Gibson G R,Rastall R A.Carbohydrate preference,acid tolerance and bile tolerance in five strains of Bifidobacterium[J].Journal of Applied Microbiology,2006,100(4):846-853
[53]Khaskheli G B,Zuo F L,Yu R,et al.Overexpression of small heat shock protein enhances heat-and salt-stress tolerance of Bifidobacterium longum NCC2705[J].Current Microbiology,2015,71(1):8-15
[54]Ventura M,Kenny J G,Zhang Z,et al.The clp B gene of Bifidobacterium breve UCC 2003:transcriptional analysis and first insights into stress induction[J].Microbiology,2005,151(9):2861-2872
[55]Zomer A,Fernandez M,Kearney B,et al.An interactive regulatory network controls stress response in Bifidobacterium breve UCC2003[J].Journal of Bacteriology,2009,191(2):7039-7049
[56]Nakamoto H,Vigh L.The small heat shock proteins and their clients[J].Cellular and Molecular Life Sciences,2007,64(3):294-306
[57]Guan N,Li J,Shin H,et al.Microbial response to environmental stresses:from fundamental mechanisms to practical applications[J].Applied Microbiology and Biotechnology,2017,101(10):3991-4008
[58]Coucheney F,Desroche N,Bou M,et al.A new approach for selection of Oenococcus oeni strains in order to produce malolactic starter[J].International Journal of Food Microbiology,2005,105(3):463-470
[59]Coucheney F,Gal L,Beney L,et al.A small HSP,Lo18interacts with the cell membrane and modulates lipid physical state under heat shock conditions in a lactic acid bacterium[J].Biochimica Et Biophysica Acta,2005,1720(1):92-98
[60]Li Y,Xu X,Qu R,et al.Heterologous expression of Oenococcus oeni s HSP20 confers temperature stress tolerance in Escherichia coli[J].Cell Stress and Chaperones,2018,23(4):653-662