核酸适配体及其在食品安全领域中的应用研究进展
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摘要
文章对适配体的本质、作用机理、特点等进行了介绍,同时从固定对象及库的构成角度分类介绍了四大类适配体筛选技术,最后,就适配体在食品安全领域中的应用现状和发展方向作一总结和展望。
This review mainly focuses on the progress on the application of aptamers on food safety detection.Firstly,the nature,mechanism and characteristics of aptamers were introduced;Secondly,four types of SELEX technology from the angle of the fixed objects and the composition of library were classified;finally,the application and development of aptamers in food safety detection were reviewed and prospected.
This review mainly focuses on the progress on the application of aptamers on food safety detection.Firstly,the nature,mechanism and characteristics of aptamers were introduced;Secondly,four types of SELEX technology from the angle of the fixed objects and the composition of library were classified;finally,the application and development of aptamers in food safety detection were reviewed and prospected.
引文
[1]江南大学国家社科重大课题《食品安全风险社会共治研究》研究团队.1-3月我国食品安全事件分析[N].中国食品安全报,2016-5-12(A2).
[2]满燕,吕雪飞,张玉奎,等.核酸适配体及其在生物医学研究中的应用[J].航天医学与医学工程,2013,26(6):485-490.
[3]GOLD L.Conformational properties of oligonucleotides[J].Nucleic Acids Symposium,1995(33):20-22.
[4]杨歌,魏强,赵新颖,等.蛋白质的核酸适配体筛选的研究进展[J].色谱,2016,34(4):370-381.
[5]刘品多,屈锋.全细胞的核酸适配体筛选的研究进展[J].色谱,2016(4):382-388.
[6]于寒松,隋佳辰,代佳宇,等.核酸适配体技术在食品重金属检测中的应用研究进展[J].食品科学,2015,36(15):228-233.
[7]王周平,张维潇.适配体及其研究进展[J].食品与生物技术学报,2013,32(9):897-906.
[8]ELLINGTON A D,SZOSTAK J W.In vitro selection of RNA molecules that bind specific ligands[J].Nature,1990,346:818-822.
[9]HERMANN T,PATEL DJ.Biochemistry-Adaptive recognition by nucleic acid aptamer[J].Science,2000,287(5454):820-825.
[10]段诺.食源性致病菌适配体的筛选及分析应用研究[D].无锡:江南大学,2013:5.
[11]MCKEAGUE M,MCCONNELL E M,CRUZ-TOLEDO J,et al.Analysis of in vitro aptamer selection parameters[J].Journal of Molecular Evolution,2015,81(5/6):150-161.
[12]徐发良,肖觉.SELEX技术的靶标范围拓展[J].第二军医大学学报,2012,33(4):432-435.
[13]TUERK C,GOLD L.Systematic evolution of ligands by exponential enrichment:RNA ligands to bacteriophage T4 DNA polymerase[J].Science,1990,249:505-510.
[14]JACOBSON O,YAN Xue-feng,NIU G,et al.PET imaging of tenascin-C with a radio labeled single-stranded DNA aptamer[J].Journal of Nuclear Medicine,2015,56(4):616-621.
[15]ZENG Zi-hua,PAREKH Parag,LI Zheng,et al.Specific and sensitive tumor imaging using biostable oligonucleotide aptamer probes[J].Theranostics,2014,4(9):945-952.
[16]LE TRINH T,ZHU Gui-zhi,XIAO Xi-lin,et al.A synthetic aptamer-drug adduct for targeted liver cancer therapy[J].PLOS ONE,2015,10(11):doi:10.1371/journa.pone.0136673.
[17]NG E W M,SHIMA D T,CALIAS P,et al.Pegaptanib,a targeted anti-VEGF aptamer for ocular vascular disease[J].Nature Reviews Drug Discovery,2006,5(2):123-132.
[18]HUANG Yu-kun,CHEN Xiu-juan,WU Shi-jia,et al.Homogeneous time-resolved fluorescence assay for the detection of ricin using an aptamer immobilized on europium-doped KGdF4nanoparticles and graphene oxide as a quencher[J].Microchimica Acta,2015,182(5/6):1 035-1 043.
[19]朱亚宝,刘晓富,翟丽芳,等.细菌耐药性与抗生素的合理使用[J].检验医学与临床,2010,7(3):286-288.
[20]NIAZI J H,LEE Su-jin,GU Man-bock.Single-stranded DNA aptamers specific for antibiotics tetracyclines[J].Bioorganic&Medicinal Chemistry,2008,16(15):7 245-7 253.
[21]STOLTENBURG R,NIKOLAUS N,STREHLITZ B.Capture-SELEX:Selection of DNA aptamers for Aminoglycoside antibiotics[J].Journal of Analytical Methods in Chemistry,2012(1):155-166.
[22]KWON M,CHUN S M,JEONG S,et al.In vitro selection of RNA against kanamycin B[J].Molecules and Celles,2001,11(3):303-311.
[23]ZHOU Nan-di,WANG Jing-yuan,ZHANG Juan,et al.Selection and identification of streptomycin-specific single-stranded DNA aptamers and the application in the detection of streptomycin in honey[J].Talanta,2013,108:109-116.
[24]DUAN Nuo,WU Shi-jia,CHEN Xiu-juan,et al.Selection and characterization of aptamers against salmonella typhimurium using whole-bacterium systemic evolution of ligands by exponential enrichment(SELEX)[J].J.Agric.Food Chem.,2013,61:3 229-3 234.
[25]LEE YJ,HAN SR,MAENG JS,et al.In vitro selection of Escherichia coli O157:H7-specific RNA aptamer[J].Biochemical and Biophysical Research Communications,2012,417(1):414-420.
[26]HAN SR,LEE SW.In vitro selection of RNA aptamer specific to Staphylococcus aureus[J].Annals of Microbiology,2014,64(2):883-885.
[27]DUAN Nuo,WU Shi-jia,CHEN Xiu-juan,et al.Selection and identification of a DNA aptamer targeted to Vibrio parahaemolyticus[J].J.Agr.Food Chem.,2012,60:4 034-4 038.
[28]DUAN Nuo,DING Xiao-ying,HE liang-xing,et al.Selection,identification and application of a DNA aptamer against Listeria monocytogenes[J].Food Control,2013,33(1):239-243.
[29]韩晓晓.阪崎肠杆菌适配体制备及其应用[D].无锡:江南大学,2013:17.
[30]WU Yuan-gen,ZHAN Shen-shan,WANG Lu-mei,et al.Selection of a DNA aptamer for cadmium detection based on cationic polymer mediated aggregation of gold nanoparticles[J].Aanlyst,2014,139(6):1 550-1 561.
[31]CHEN Zheng-bo,CHEN Liang,MA He,et al.Aptamer biosensor for label-free impedance spectroscopy detection of potassiumion based on DNA G-quadruplex conformation[J].Biosensors and Bioelectronics,2013,48:108-112.
[32]SVEN Klussmann.核酸适配体手册:功能性寡核苷酸及其应用[M].屈锋,译.北京:化学工业出版社,2013:84-99.
[33]陈秀娟.镰刀菌毒素核酸适配体的筛选及分析应用研究[D].无锡:江南大学,2015:44,60.
[34]NUTIU R,LI Ying-fu.In vitro selection of structureswitching signaling aptamers[J].Angewandte Chemie-International Edition,2005,44(7):1 061-1 065.
[35]刘晓静.SELEX技术筛选人TGF-b RⅡ亲和核酸库方法的建立[D].重庆:第三军医大学,2004:12-19.
[36]MENDONSA S D,BOWSER M T.In vitro evolution of functional DNA using capillary electrophoresis[J].Journal of the American Chemical Society,2004,126(1):20-21.
[37]GU Hua-jie,DUAN Nuo,WU Shi-jia,et al.Graphene oxideassisted non-immobilized SELEX of okdaic acid aptamer and the analytical application of aptasensor[J].Scientific Reports,2016,6:doi:10.1038/srep21665.
[38]BENNER S A.Understanding nucleic acids using synthetic chemistry[J].Accounts of Chemical Research,2004,37(10):784-797.
[39]SEFAH K,YANG Zun-yi,BRADLEY K M,et al.In vitro selection with artificial expanded genetic information systems[J].PNAS,2014,111(4):1 449-1 454.
[40]JOSHI R,JANAGAMA H,DWIVEDI HP,et al.Selection,characterization,and application of DNA aptamers for the capture and detection of Salmonella enterica serovars[J].Molecular and Cellular Probes,2009,23(1):20-28.
[41]DUAN Nuo,WU Shi-jia,CHEN Xiu-juan,et al.Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium systemic evolution of ligands by exponential enrichment(SELEX)[J].Jouranl of Agricultural and Food Chemistry,2013,61:3 229-3 234.
[42]DUAN Nuo,YAN Yi-ling,WU Shi-jia,et al.Vibrio parahaemolyticus detection aptasensor using surface-enhanced Raman scattring[J].Food Control,2016,63:122-127.
[43]CRUZ-AGUADO JA,PENNER G.Determination of Ochratoxin A with a DNA aptamer[J].Journal of Agricultural and Food Chemistry,2008,56(22):10 456-10 461.
[44]段诺,吴世嘉,王周平.核酸适配体识别荧光法检测赭曲霉毒素A[J].分析化学,2011,39(3):300-304.
[45]PARK J H,BYUN J Y,MUN H,et al.A regeneratable,label-free,localized surface Plasmon resonance(LSPR)aptasensor for the detection of ochratoxin A[J].Biosensors&Bioelectronics,2014,59:321-327.
[46]HUANG Yu-kun,CHEN Xiu-juan,XIA Yu,et al.Selection,identification and application of a DNA aptamer against staphylococcus aureus enterotoxin A[J].Analytical Methods,2014,6(3):690-697.
[47]HUANG Yu-kun,CHEN Xiu-juan,DUAN Nuo,et al.Selection and characterization of DNA aptamers against staphylococcus aureus enterotoxin C1[J].Food Chemistry,2015,166:623-629.
[48]BRUNO J G,KIEL J L.Use of magnetic beads in selection and detection of biotoxin aptamers by electrochemiluminescence and enzymatic methods[J].Biotechniques,2002,32(1):178-180,182-183.
[49]HAES A J,GIORDANO B C,COLLINS G E.Aptamerbased detection and quantitative analysis of ricin using affinity probe capillary electrophoresis[J].Analytical Chemistry,2006,78(11):3 758-3 764.
[50]MIYAKE Y,TOGASHI H,TASHIRO M,et al.MercuryⅡ-mediated formation of thymine-HgⅡ-Thymine base paris in DNA duplexes[J].Journal of the American Chemical Society,2006,128(7):2 172-2 173.
[51]TANAKA Y,ODA S,YAMAGUCHI H,et al.15N-15NJcoupling across HgⅡ:Direct observation of HgⅡ-Medeated TT base pairs in a DNA duplex[J].Journal of the American Chemical Society,2006,129(2):244-245.
[52]WANG Yong,YANG Fan,YANG Xiu-rong.Colorimetric biosensing of mercuryⅡion using unmodified gold nanoparticle probes and thrombin-binding aptamer[J].Biosensors&Bioelectronics,2010,25(8):1 994-1 998.
[53]凌绍明,范燕燕,蒋治良,等.核酸适配体修饰纳米金共振散射光谱探针快速检测痕量Pb2+[J].化学学报,2010,68(4):339-344.
[54]KIM M,UM H J,BANG S,et al.Arsenic removal from Vietnamese groundwater using the arsenic-binding DNA aptamer[J].Environmental Science&Technology,2009,43(24):9 335-9 340.
[55]WU Shi-jia,DUAN Nuo,SHI Zhao,et al.Dual fluorescence resonance energy transfer assay between tunable upconversion nanoparticles and controlled gold nanoparticles for the simultaneous detection of Pb2+and Hg2+[J].Chemistry Aanlytical,2014,128:327-336.
[56]廖且根,李伟红,张金艳,等.基于核酸适配体与银纳米粒子探针共振光散射技术检测卡那霉素[J].农产品质量与安全,2012(S1):17-19.
[57]YADAV S K,AGRAWAL B,CHANDRA P,et al.In vitro chloramphenicol detection in a Haemophilus influenza model using an aptamer-polymer based electrochemical biosensor[J].Biosensors&Bioelectronics,2014,55:337-342.
[58]WANG Li,LIU Xian-jin,ZHANG Qiang,et al.Selection of DNA aptamers that bind to four organophosphorus pesticides[J].Biotechnology Letters,2012,34(5):869-874.
[2]满燕,吕雪飞,张玉奎,等.核酸适配体及其在生物医学研究中的应用[J].航天医学与医学工程,2013,26(6):485-490.
[3]GOLD L.Conformational properties of oligonucleotides[J].Nucleic Acids Symposium,1995(33):20-22.
[4]杨歌,魏强,赵新颖,等.蛋白质的核酸适配体筛选的研究进展[J].色谱,2016,34(4):370-381.
[5]刘品多,屈锋.全细胞的核酸适配体筛选的研究进展[J].色谱,2016(4):382-388.
[6]于寒松,隋佳辰,代佳宇,等.核酸适配体技术在食品重金属检测中的应用研究进展[J].食品科学,2015,36(15):228-233.
[7]王周平,张维潇.适配体及其研究进展[J].食品与生物技术学报,2013,32(9):897-906.
[8]ELLINGTON A D,SZOSTAK J W.In vitro selection of RNA molecules that bind specific ligands[J].Nature,1990,346:818-822.
[9]HERMANN T,PATEL DJ.Biochemistry-Adaptive recognition by nucleic acid aptamer[J].Science,2000,287(5454):820-825.
[10]段诺.食源性致病菌适配体的筛选及分析应用研究[D].无锡:江南大学,2013:5.
[11]MCKEAGUE M,MCCONNELL E M,CRUZ-TOLEDO J,et al.Analysis of in vitro aptamer selection parameters[J].Journal of Molecular Evolution,2015,81(5/6):150-161.
[12]徐发良,肖觉.SELEX技术的靶标范围拓展[J].第二军医大学学报,2012,33(4):432-435.
[13]TUERK C,GOLD L.Systematic evolution of ligands by exponential enrichment:RNA ligands to bacteriophage T4 DNA polymerase[J].Science,1990,249:505-510.
[14]JACOBSON O,YAN Xue-feng,NIU G,et al.PET imaging of tenascin-C with a radio labeled single-stranded DNA aptamer[J].Journal of Nuclear Medicine,2015,56(4):616-621.
[15]ZENG Zi-hua,PAREKH Parag,LI Zheng,et al.Specific and sensitive tumor imaging using biostable oligonucleotide aptamer probes[J].Theranostics,2014,4(9):945-952.
[16]LE TRINH T,ZHU Gui-zhi,XIAO Xi-lin,et al.A synthetic aptamer-drug adduct for targeted liver cancer therapy[J].PLOS ONE,2015,10(11):doi:10.1371/journa.pone.0136673.
[17]NG E W M,SHIMA D T,CALIAS P,et al.Pegaptanib,a targeted anti-VEGF aptamer for ocular vascular disease[J].Nature Reviews Drug Discovery,2006,5(2):123-132.
[18]HUANG Yu-kun,CHEN Xiu-juan,WU Shi-jia,et al.Homogeneous time-resolved fluorescence assay for the detection of ricin using an aptamer immobilized on europium-doped KGdF4nanoparticles and graphene oxide as a quencher[J].Microchimica Acta,2015,182(5/6):1 035-1 043.
[19]朱亚宝,刘晓富,翟丽芳,等.细菌耐药性与抗生素的合理使用[J].检验医学与临床,2010,7(3):286-288.
[20]NIAZI J H,LEE Su-jin,GU Man-bock.Single-stranded DNA aptamers specific for antibiotics tetracyclines[J].Bioorganic&Medicinal Chemistry,2008,16(15):7 245-7 253.
[21]STOLTENBURG R,NIKOLAUS N,STREHLITZ B.Capture-SELEX:Selection of DNA aptamers for Aminoglycoside antibiotics[J].Journal of Analytical Methods in Chemistry,2012(1):155-166.
[22]KWON M,CHUN S M,JEONG S,et al.In vitro selection of RNA against kanamycin B[J].Molecules and Celles,2001,11(3):303-311.
[23]ZHOU Nan-di,WANG Jing-yuan,ZHANG Juan,et al.Selection and identification of streptomycin-specific single-stranded DNA aptamers and the application in the detection of streptomycin in honey[J].Talanta,2013,108:109-116.
[24]DUAN Nuo,WU Shi-jia,CHEN Xiu-juan,et al.Selection and characterization of aptamers against salmonella typhimurium using whole-bacterium systemic evolution of ligands by exponential enrichment(SELEX)[J].J.Agric.Food Chem.,2013,61:3 229-3 234.
[25]LEE YJ,HAN SR,MAENG JS,et al.In vitro selection of Escherichia coli O157:H7-specific RNA aptamer[J].Biochemical and Biophysical Research Communications,2012,417(1):414-420.
[26]HAN SR,LEE SW.In vitro selection of RNA aptamer specific to Staphylococcus aureus[J].Annals of Microbiology,2014,64(2):883-885.
[27]DUAN Nuo,WU Shi-jia,CHEN Xiu-juan,et al.Selection and identification of a DNA aptamer targeted to Vibrio parahaemolyticus[J].J.Agr.Food Chem.,2012,60:4 034-4 038.
[28]DUAN Nuo,DING Xiao-ying,HE liang-xing,et al.Selection,identification and application of a DNA aptamer against Listeria monocytogenes[J].Food Control,2013,33(1):239-243.
[29]韩晓晓.阪崎肠杆菌适配体制备及其应用[D].无锡:江南大学,2013:17.
[30]WU Yuan-gen,ZHAN Shen-shan,WANG Lu-mei,et al.Selection of a DNA aptamer for cadmium detection based on cationic polymer mediated aggregation of gold nanoparticles[J].Aanlyst,2014,139(6):1 550-1 561.
[31]CHEN Zheng-bo,CHEN Liang,MA He,et al.Aptamer biosensor for label-free impedance spectroscopy detection of potassiumion based on DNA G-quadruplex conformation[J].Biosensors and Bioelectronics,2013,48:108-112.
[32]SVEN Klussmann.核酸适配体手册:功能性寡核苷酸及其应用[M].屈锋,译.北京:化学工业出版社,2013:84-99.
[33]陈秀娟.镰刀菌毒素核酸适配体的筛选及分析应用研究[D].无锡:江南大学,2015:44,60.
[34]NUTIU R,LI Ying-fu.In vitro selection of structureswitching signaling aptamers[J].Angewandte Chemie-International Edition,2005,44(7):1 061-1 065.
[35]刘晓静.SELEX技术筛选人TGF-b RⅡ亲和核酸库方法的建立[D].重庆:第三军医大学,2004:12-19.
[36]MENDONSA S D,BOWSER M T.In vitro evolution of functional DNA using capillary electrophoresis[J].Journal of the American Chemical Society,2004,126(1):20-21.
[37]GU Hua-jie,DUAN Nuo,WU Shi-jia,et al.Graphene oxideassisted non-immobilized SELEX of okdaic acid aptamer and the analytical application of aptasensor[J].Scientific Reports,2016,6:doi:10.1038/srep21665.
[38]BENNER S A.Understanding nucleic acids using synthetic chemistry[J].Accounts of Chemical Research,2004,37(10):784-797.
[39]SEFAH K,YANG Zun-yi,BRADLEY K M,et al.In vitro selection with artificial expanded genetic information systems[J].PNAS,2014,111(4):1 449-1 454.
[40]JOSHI R,JANAGAMA H,DWIVEDI HP,et al.Selection,characterization,and application of DNA aptamers for the capture and detection of Salmonella enterica serovars[J].Molecular and Cellular Probes,2009,23(1):20-28.
[41]DUAN Nuo,WU Shi-jia,CHEN Xiu-juan,et al.Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium systemic evolution of ligands by exponential enrichment(SELEX)[J].Jouranl of Agricultural and Food Chemistry,2013,61:3 229-3 234.
[42]DUAN Nuo,YAN Yi-ling,WU Shi-jia,et al.Vibrio parahaemolyticus detection aptasensor using surface-enhanced Raman scattring[J].Food Control,2016,63:122-127.
[43]CRUZ-AGUADO JA,PENNER G.Determination of Ochratoxin A with a DNA aptamer[J].Journal of Agricultural and Food Chemistry,2008,56(22):10 456-10 461.
[44]段诺,吴世嘉,王周平.核酸适配体识别荧光法检测赭曲霉毒素A[J].分析化学,2011,39(3):300-304.
[45]PARK J H,BYUN J Y,MUN H,et al.A regeneratable,label-free,localized surface Plasmon resonance(LSPR)aptasensor for the detection of ochratoxin A[J].Biosensors&Bioelectronics,2014,59:321-327.
[46]HUANG Yu-kun,CHEN Xiu-juan,XIA Yu,et al.Selection,identification and application of a DNA aptamer against staphylococcus aureus enterotoxin A[J].Analytical Methods,2014,6(3):690-697.
[47]HUANG Yu-kun,CHEN Xiu-juan,DUAN Nuo,et al.Selection and characterization of DNA aptamers against staphylococcus aureus enterotoxin C1[J].Food Chemistry,2015,166:623-629.
[48]BRUNO J G,KIEL J L.Use of magnetic beads in selection and detection of biotoxin aptamers by electrochemiluminescence and enzymatic methods[J].Biotechniques,2002,32(1):178-180,182-183.
[49]HAES A J,GIORDANO B C,COLLINS G E.Aptamerbased detection and quantitative analysis of ricin using affinity probe capillary electrophoresis[J].Analytical Chemistry,2006,78(11):3 758-3 764.
[50]MIYAKE Y,TOGASHI H,TASHIRO M,et al.MercuryⅡ-mediated formation of thymine-HgⅡ-Thymine base paris in DNA duplexes[J].Journal of the American Chemical Society,2006,128(7):2 172-2 173.
[51]TANAKA Y,ODA S,YAMAGUCHI H,et al.15N-15NJcoupling across HgⅡ:Direct observation of HgⅡ-Medeated TT base pairs in a DNA duplex[J].Journal of the American Chemical Society,2006,129(2):244-245.
[52]WANG Yong,YANG Fan,YANG Xiu-rong.Colorimetric biosensing of mercuryⅡion using unmodified gold nanoparticle probes and thrombin-binding aptamer[J].Biosensors&Bioelectronics,2010,25(8):1 994-1 998.
[53]凌绍明,范燕燕,蒋治良,等.核酸适配体修饰纳米金共振散射光谱探针快速检测痕量Pb2+[J].化学学报,2010,68(4):339-344.
[54]KIM M,UM H J,BANG S,et al.Arsenic removal from Vietnamese groundwater using the arsenic-binding DNA aptamer[J].Environmental Science&Technology,2009,43(24):9 335-9 340.
[55]WU Shi-jia,DUAN Nuo,SHI Zhao,et al.Dual fluorescence resonance energy transfer assay between tunable upconversion nanoparticles and controlled gold nanoparticles for the simultaneous detection of Pb2+and Hg2+[J].Chemistry Aanlytical,2014,128:327-336.
[56]廖且根,李伟红,张金艳,等.基于核酸适配体与银纳米粒子探针共振光散射技术检测卡那霉素[J].农产品质量与安全,2012(S1):17-19.
[57]YADAV S K,AGRAWAL B,CHANDRA P,et al.In vitro chloramphenicol detection in a Haemophilus influenza model using an aptamer-polymer based electrochemical biosensor[J].Biosensors&Bioelectronics,2014,55:337-342.
[58]WANG Li,LIU Xian-jin,ZHANG Qiang,et al.Selection of DNA aptamers that bind to four organophosphorus pesticides[J].Biotechnology Letters,2012,34(5):869-874.