漆酶活性对石油污染土壤特性和微生物活性的影响
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摘要
对土壤的石油污染问题进行分析,通过30d的花盆模拟实验,研究了不同漆酶活性对石油污染土壤特性及石油烃降解等土壤微生物活性的影响。结果表明:当土壤中漆酶活性为12U/g时,石油烃降解效果最佳,修复30d后石油烃降解率达到19.00%,比不调节漆酶活性的对照组提高了10.50百分点;漆酶活性提高有利于土壤持水性能保持,可以促进微生物的生长代谢及土壤中脱氢酶、过氧化氢酶的活性,进而促进石油污染土壤的生物修复效果。
Based on the problem of oil-contaminated soil,and by regulating the activity of laccase in soil,the effect of different laccase activity on the characteristics of oil-contaminated soil and microbial activity of oil-contaminated soil including the degradation of oil during the bio-remediation process during 30 dwhich was conducted in flowerpots were investigated.The result showed that:when the activity of laccase was 12 U/g,the effect of oil degradation was the best and after 30 d degradation the degradation rate could reach 19.00 percent,which advanced about 10.50 percent as compared to the control group without regulating the activity of laccase in soil.Improvement of laccase activity is beneficial to the preservation of water retention property,and can promote microbial growth and metabolism. The improvement can also enhance the activity of dehydrogenation and catalysis in oil-contaminated soil. All these influences will boost the bio-remediation of oil-contaminated soil.
Based on the problem of oil-contaminated soil,and by regulating the activity of laccase in soil,the effect of different laccase activity on the characteristics of oil-contaminated soil and microbial activity of oil-contaminated soil including the degradation of oil during the bio-remediation process during 30 dwhich was conducted in flowerpots were investigated.The result showed that:when the activity of laccase was 12 U/g,the effect of oil degradation was the best and after 30 d degradation the degradation rate could reach 19.00 percent,which advanced about 10.50 percent as compared to the control group without regulating the activity of laccase in soil.Improvement of laccase activity is beneficial to the preservation of water retention property,and can promote microbial growth and metabolism. The improvement can also enhance the activity of dehydrogenation and catalysis in oil-contaminated soil. All these influences will boost the bio-remediation of oil-contaminated soil.
引文
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[12]陈辉,张剑波,王维敬,等.固定化漆酶催化去除水中2,4-二氯苯酚[J].北京大学学报(自然科学版)网络版,2006,1(1):1-6.(CHEN Hui,ZHANG Jianbo,WANGWeijing,et al.Degradation of 2,4-dichlorophenols catalyzed by laccase immobilized on chitosan[J].Acta Scientriarum Naturalium Universitatis Pekinensison Line,2006,1(1):1-6.)
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[16]蒋瑞萍,孙丽丽,解开治,等.采用紫外分光光度法评价石油烃降解菌的降解能力[J].石化技术与应用,2015,33(1):70-74.(JIANG Ruiping,SUN Lili,XIE Kaizhi,et al.Evaluation of degrading ability of petroleum hydrocarbon degrading bacteria by ultraviolet spectrophotometry[J].Petrochemical Technology&Application,2015,33(1):70-74.)
[17]张育淇.有机功能化SiO2大孔材料固定化漆酶及其应用[D].宁波:宁波大学,2014.
[18]付辉,孟瑶,梁红,等.复合白腐真菌菌剂修复原油污染土壤[J].森林工程,2012,28(4):17-20.(FU Hui,MENG Yao,LIANG Hong,et al.Restoration of oil polluted soil using combined fungus of white-rot fungi and microbial inoculums[J].Forest Engineering,2012,28(4):17-20.)
[19]孟娟,郑西来.营养添加法治理石油污染土壤研究进展[J].现代化工,2016,36(3):49-52.(MENG Juan,ZHENG Xilai.Research progress of treatment of oil contaminated soil by nutrient addition method[J].Modern Chemical Industry,2016,36(3):49-52.)
[20]盛恩国,徐海,蓝江湖,等.青藏高原东部边缘泥炭腐殖化度作为评价有机质含量的指标研究[J].地球与环境,2013,41(1):37-42.(SHENG Enguo,XU Hai,LAN Jianghu,et al.Study of humification degree for estimating organic matter contents of peat on the eastern margin of the Tibetan Plateau[J].Earth and Environment,2013,41(1):37-42.)
[21]张秀霞,韩雨彤,张涵,等.腐植酸对石油污染土壤特性和生物修复效果的影响[J].石油学报(石油加工),2016,32(1):164-169.(ZHANG Xiuxia,HAN Yutong,ZHANG Han,et al.Influence of humic acid on the soil characteristics and bio-remediation of oilcontaminated soil[J].Acta Petrolei Sinica(Petroleum Processing Section),2016,32(1):164-169.)
[22]吴伟林,张秀霞,单宝来,等.不同处置方式对石油污染土壤理化性质和生物学特性的影响[J].石油学报(石油加工),2010,26(5):831-834.(WU Weilin,ZHANG Xiuxia,SHAN Baolai,et al.Effect of different treatment methods on the physicochemical and biochemical properties of an oil polluted soil[J].Acta Petrolei Sinica(Petroleum Processing Section),2010,26(5):831-834.)
[23]AHN M Y,DEC J,KIM J E,et al.Treatment of2,4-dichlorophenol polluted soil with free and immobilized laccase[J].Journal of Environmental Quality,2002,31(5):1509-1516.
[2]邓绍云,徐学义,邱清华.我国石油污染土壤修复研究现状与展望[J].北方园艺,2012,(14):184-190.(DENG Shaoyun,XU Xueyi,QIU Qinghua.Present situation and prospect of research of remediation in oilcontaminated soil[J].Northern Horticulture,2012,(14):184-190.)
[3]CHANDRA S.Application of bioremediation technology in the environment contaminated with petroleum hydrocarbon[J].Ann Microbiol,2013,63:417-431.
[4]ZHOU M F,YUAN X Z,ZHONG H,et al.Effect of biosurfactants on laccase production and phenol biodegradation in solid-state fermentation[J].Applied Biochemistry and Biotednology,2010,164:103-114.
[5]GUO H,YAO J,CAI M M,et al.Effects of petroleum contamination on soil microbial numbers,metabolic activity and urease activity[J].Chemosphere,2012,87(11):1273-1280.
[6]EIBES G,ARCA-RAMOS A,FEIJOO G,et al.Enzymatic technologies for remediation of hydrophobic organic pollutants in soil[J].Application Microbiological Biotechnology,2015,99:8815-8829.
[7]王华金,朱能武,杨崇,等.石油污染土壤生物修复对土壤酶活性的影响[J].农业环境科学学报,2013,32(6):1178-1184.(WANG Huajin,ZHU Nengwu,YANG Chong,et al.Effect of soil enzyme actives during bioremediation of crude oil-contaminated soil[J].Journal of Agro-Environment Science,2013,32(6):1178-1184.)
[8]曹明乐,张海波,黄峰,等.漆酶的研究进展[J].山东林业科技,2012,(3):106-115,127.(CAO Mingle,ZHANG Haibo,HUANG Feng,et al.Research progress of laccase[J].Shandong Forestry Science and Technology,2012,(3):106-115,127.)
[9]高恩丽.云芝漆酶的生产及其应用基础研究[D].杭州:浙江大学,2007.
[10]刘家扬.白腐真菌Pycnoporus sp.SYBC-L3产漆酶及应用研究[D].无锡:江南大学,2013.
[11]ZHAO Y C,YI X Y,ZHANG M,et al.Fundamental study of degradation of dichlorodiphenyltrichloroethane in soil by laccase from white rot fungi[J].Environmental Science Technology,2010,7(2):359-366.
[12]陈辉,张剑波,王维敬,等.固定化漆酶催化去除水中2,4-二氯苯酚[J].北京大学学报(自然科学版)网络版,2006,1(1):1-6.(CHEN Hui,ZHANG Jianbo,WANGWeijing,et al.Degradation of 2,4-dichlorophenols catalyzed by laccase immobilized on chitosan[J].Acta Scientriarum Naturalium Universitatis Pekinensison Line,2006,1(1):1-6.)
[13]Chang Y T,Lee J F,Liu K H,et al.Immobilization of fungal laccase onto a nonionic surfactant-modified clay material:Application to PAH degradation[J].Environmental Science and Pollution Research,2015,23(5):1-12.
[14]陈新芳.油对水质稳定剂性能的影响及酶法处理含有污水的研究[D].北京:北京化工大学,2007.
[15]贾洪柏,曲丽娜,王秋玉.真菌漆酶活性与石油降解率的关系[J].安徽农业科学,2014,42(7):2097-2100.(JIA Hongbai,QU Lina,WANG Qiuyu.Study on the relationship between laccase activity and crude-oil degradation rate of the fungus[J].Journal of Anhui Agricultural Science,2014,42(7):2097-2100.)
[16]蒋瑞萍,孙丽丽,解开治,等.采用紫外分光光度法评价石油烃降解菌的降解能力[J].石化技术与应用,2015,33(1):70-74.(JIANG Ruiping,SUN Lili,XIE Kaizhi,et al.Evaluation of degrading ability of petroleum hydrocarbon degrading bacteria by ultraviolet spectrophotometry[J].Petrochemical Technology&Application,2015,33(1):70-74.)
[17]张育淇.有机功能化SiO2大孔材料固定化漆酶及其应用[D].宁波:宁波大学,2014.
[18]付辉,孟瑶,梁红,等.复合白腐真菌菌剂修复原油污染土壤[J].森林工程,2012,28(4):17-20.(FU Hui,MENG Yao,LIANG Hong,et al.Restoration of oil polluted soil using combined fungus of white-rot fungi and microbial inoculums[J].Forest Engineering,2012,28(4):17-20.)
[19]孟娟,郑西来.营养添加法治理石油污染土壤研究进展[J].现代化工,2016,36(3):49-52.(MENG Juan,ZHENG Xilai.Research progress of treatment of oil contaminated soil by nutrient addition method[J].Modern Chemical Industry,2016,36(3):49-52.)
[20]盛恩国,徐海,蓝江湖,等.青藏高原东部边缘泥炭腐殖化度作为评价有机质含量的指标研究[J].地球与环境,2013,41(1):37-42.(SHENG Enguo,XU Hai,LAN Jianghu,et al.Study of humification degree for estimating organic matter contents of peat on the eastern margin of the Tibetan Plateau[J].Earth and Environment,2013,41(1):37-42.)
[21]张秀霞,韩雨彤,张涵,等.腐植酸对石油污染土壤特性和生物修复效果的影响[J].石油学报(石油加工),2016,32(1):164-169.(ZHANG Xiuxia,HAN Yutong,ZHANG Han,et al.Influence of humic acid on the soil characteristics and bio-remediation of oilcontaminated soil[J].Acta Petrolei Sinica(Petroleum Processing Section),2016,32(1):164-169.)
[22]吴伟林,张秀霞,单宝来,等.不同处置方式对石油污染土壤理化性质和生物学特性的影响[J].石油学报(石油加工),2010,26(5):831-834.(WU Weilin,ZHANG Xiuxia,SHAN Baolai,et al.Effect of different treatment methods on the physicochemical and biochemical properties of an oil polluted soil[J].Acta Petrolei Sinica(Petroleum Processing Section),2010,26(5):831-834.)
[23]AHN M Y,DEC J,KIM J E,et al.Treatment of2,4-dichlorophenol polluted soil with free and immobilized laccase[J].Journal of Environmental Quality,2002,31(5):1509-1516.