miR-137与胃癌侵袭能力的关系研究
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摘要
目的:探讨胃癌组织mi R-137的表达及其与胃癌细胞侵袭能力间的关系。方法:用q RT-PCR检测胃癌组织与癌旁组织mi R-137的表达,分析mi R-13表达与胃癌临床病理特征的关系;分别用q RT-PCR与Transwell侵袭实验检测3种胃癌细胞(AGS、SGC7901、BGC823)及正常胃黏膜细胞(GES1)mi R-137的表达与穿膜。结果:胃癌组织中mi R-137表达量明显低于癌旁组织(P<0.05),mi R-137表达与胃癌患者T分期有关,T分期越高mi R-137表达量越低(P<0.05);各胃癌细胞mi R-137表达量均低于正常胃黏膜细胞,且胃癌细胞侵袭力越强胃癌细胞mi R-137表达量越低(P<0.05);穿膜细胞数与mi R-137表达量之间呈明显负相关(r=-0.8881,P<0.05)。结论:胃癌组织mi R-137表达降低,且mi R-137表达量越低提示胃癌细胞侵袭能力越强。
引文
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[31]Le XF,Merchant O,Bast RC,et al.The Roles of Micro RNAs in the Cancer Invasion-Metastasis Cascade[J].Cancer Microenviron,2010,3(1):137-147.
[32]Klppel G,Rindi G,Perren A,et al.The ENETS and AJCC/UICC TNM classifications of the neuroendocrine tumors of the gastrointestinal tract and the pancreas:a statement[J].Virchows Arch,2010,456(6):595-597.
[33]Deng Y,Deng H,Bi F,et al.Micro RNA-137 targets carboxylterminal binding protein 1 in melanoma cell lines[J].Int J Biol Sci,2011,7(1):133-137.
[34]Spizzo R,Nicoloso MS,Croce CM,et al.Snap Shot:Micro RNAs in Cancer[J].Cell,2009,137(3):586.
[35]王中欣.MMP-9和CD44v6在大肠癌中的表达及其临床意义[J].中国普通外科杂志,2012,21(6):743-745.
[36]侯福涛,陈子华,钱立元.BMI-1和MMP-9在结肠癌细胞中的表达及意义[J].中国普通外科杂志,2010,19(10):1103-1106.
[2]周兴舰,李红浪.Micro RNA功能异常与肿瘤浸润转移的关系[J].中国普通外科杂志,2012,21(5):602-606.
[3]Bier A,GiladiN,Kronfeld N,et al.Mdownregulated in lioblastoma and inhibits the stemness of glioma stem cells by targeting RTVP-1[J].Oncotarget,2013,4(5):665-676.
[4]Karsy M,Arslan E,Moy F.Current Progress on Understanding Micro RNAs in Glioblastoma Multiforme[J].Genes Cancer,2012,3(1):3-15.
[5]Sun J,Gong X,Purow B,et al.and Transcription Factor Mediated Regulatory Networks in Glioblastoma[J].PLo S Comput Biol,2012,8(7):e1002488.doi:10.1371/journal.pcbi.1002488.
[6]Tunca B,Tezcan G,Cecener G,et al.Olea europaea leaf extract alters micro RNA expression in human glioblastoma cells[J].J Cancer Res Clin Oncol,2012,138(11):1831-1844.
[7]Chen L,Wang X,Wang H,et al.mi R-137 is frequently downregulated in glioblastoma and is a negative regulator of Cox-2[J].Eur J Cancer,2012,48(16):3104-3111.
[8]Silber J,Lim DA,Petritsch C,et al.mi R-124 and mi R-137inhibit proliferation of glioblastoma multiforme cells and induce differentiation of brain tumor stem cells[J].BMC Med,2008,6:14.doi:10.1186/1741-7015-6-14.
[9]Althoff K,Beckers A,Odersky A,et al.Mi R-137 functions as a tumor suppressor in neuroblastoma by downregulating KDM1A[J].Int J Cancer,2013,133(5):1064-1073.
[10]Zhi F,Chen X,Wang S,et al.The use of hsa-mi R-21,hsa-mi R-181b and hsa-mi R-106a as prognostic indicators of astrocytoma[J].Eur J Cancer,2010,46(9):1640-1649.
[11]Li KK,Yang L,Pang JC,et al.MIR-137 suppresses growth and invasion,is downregulated in oligodendroglial tumors and targets CSE1L[J].Brain Pathol,2013,23(4):426-439.
[12]Chen Q,Chen X,Zhang M,et al.mi R-137 is frequently downregulated in gastric cancer and is a negative regulator of Cdc42[J].Dig Dis Sci,2011,56(7):2009-2016.
[13]Qu J,Zhao L,Zhang P,et al.Micro RNA-195 chemosensitizes colon cancer cells to the chemotherapeutic drug doxorubicin by targeting the first binding site of BCL2L2 m RNA[J].J Cell Physiol,2015,230(3):535-545.
[14]Chen DL,Wang DS,Wu WJ,et al.Overexpression of paxillin induced by mi R-137 suppression promotes tumor progression and metastasis in colorectal cancer[J].Carcinogenesis,2013,34(4):803-811.
[15]Liang L,Li X,Zhang X,et al.Micro RNA-137,an HMGA1 target,suppresses colorectal cancer cell invasion and metastasis in mice by directly targeting FMNL2[J].Gastroenterology,2013,144(3):624-635.
[16]Sun Y,Zhao X,Zhou Y,et al.mi R-124,mi R-137 and mi R-340regulate colorectal cancer growth via inhibition of the Warburg effect[J].Oncol Rep,2012,28(4):1346-1352.
[17]Oberg AL,French AJ,Sarver AL,et al.mi RNA expression in colon polyps provides evidence for a multihit model of colon cancer[J].PLo S One,2011,6(6):e20465.doi:10.1371/journal.pone.0020465.
[18]Necela BM,Carr JM,Asmann YW,et al.Differential expression of micro RNAs in tumors from chronically inflamed or genetic(APC(Min/+))models of colon cancer[J].PLo S One,2011,6(4):e18501.doi:10.1371/journal.pone.0018501.
[19]Liu M,Lang N,Qiu M,et al.mi R-137 targets Cdc42 expression,induces cell cycle G1 arrest and inhibits invasion in colorectal cancer cells[J].Int J Cancer,2011,128(6):1269-1279.
[20]Huang ZM,Yang J,Shen XY,et al.Micro RNA expression profile in non-cancerous colonic tissue associated with lymph node metastasis of colon cancer[J].J Dig Dis,2009,10(3):188-194.
[21]Svoboda M,Izakovicova HL,Sefr R,et al.Micro-RNAs mi R125b and mi R137 are frequently upregulated in response to capecitabine chemoradiotherapy of rectal cancer[J].Int J Oncol,2008,33(3):541-547.
[22]Khoshnaw SM,Rakha EA,Abdel-Fatah T,et al.The micro RNA maturation regulator Drosha is an independent predictor of outcome in breast cancer patients[J].Breast Cancer Res Treat,2013,137(1):139-153.
[23]Zhu X,Li Y,Shen H,et al.mi R-137 restoration sensitizes multidrug-resistant MCF-7/ADM cells to anticancer agents by targeting YB-1[J].Acta Biochim Biophys Sin(Shanghai),2013,45(2):80-86.
[24]Zhao Y,Li Y,Lou G,et al.Mi R-137 targets estrogen-related receptor alpha and impairs the proliferative and migratory capacity of breast cancer cells[J].PLo S One,2012,7(6):e39102.doi:10.1371/journal.pone.0039102.
[25]Luo C,Tetteh PW,Merz PR,et al.mi R-137 inhibits the invasion of melanoma cells through downregulation of multiple oncogenic target genes[J].J Invest Dermatol,2013,133(3):768-775.
[26]Chen X,Wang J,Shen H,et al.Epigenetics,micro RNAs,and carcinogenesis:functional role of micro RNA-137 in uveal melanoma[J].Invest Ophthalmol Vis Sci,2011,52(3):1193-1199.
[27]Zhu X,Li Y,Shen H,et al.mi R-137 inhibits the proliferation of lung cancer cells by targeting Cdc42 and Cdk6[J].FEBS Lett,2013,587(1):73-81.
[28]Tu HF,Lin SC,Chang KW.Micro RNA aberrances in head and neck cancer:pathogenetic and clinical significance[J].Curr Opin Otolaryngol Head Neck Surg,2013,21(2):104-111.
[29]Wong TS,Liu XB,Wong BY,et al.Mature mi R-184 as Potential Oncogenic micro RNA of Squamous Cell Carcinoma of Tongue[J].Clin Cancer Res,2008,14(9):2588-2592.
[30]de Cubas AA,Leandro-García LJ,Schiavi F,et al.Integrative apheochromocytoma and paraganglioma identifies genotype-specific markers and potentially regulated pathways[J].Endocr Relat Cancer,2013,20(4):477-493.
[31]Le XF,Merchant O,Bast RC,et al.The Roles of Micro RNAs in the Cancer Invasion-Metastasis Cascade[J].Cancer Microenviron,2010,3(1):137-147.
[32]Klppel G,Rindi G,Perren A,et al.The ENETS and AJCC/UICC TNM classifications of the neuroendocrine tumors of the gastrointestinal tract and the pancreas:a statement[J].Virchows Arch,2010,456(6):595-597.
[33]Deng Y,Deng H,Bi F,et al.Micro RNA-137 targets carboxylterminal binding protein 1 in melanoma cell lines[J].Int J Biol Sci,2011,7(1):133-137.
[34]Spizzo R,Nicoloso MS,Croce CM,et al.Snap Shot:Micro RNAs in Cancer[J].Cell,2009,137(3):586.
[35]王中欣.MMP-9和CD44v6在大肠癌中的表达及其临床意义[J].中国普通外科杂志,2012,21(6):743-745.
[36]侯福涛,陈子华,钱立元.BMI-1和MMP-9在结肠癌细胞中的表达及意义[J].中国普通外科杂志,2010,19(10):1103-1106.