血样采集方法对血清样品中PGE_2、PGF_(2α)检测结果的影响
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摘要
目的探讨临床血清样品不同放置条件下的放置时间对检测结果的影响。方法随机选取在校研究生女性健康志愿者1例,采集血液50 ml,平均分成16份,分别在离心前后室温放置0.5 h和2 h,离心后在4℃条件下临时存放0.5,8,24,48 h,检测其对血清中PGE_2和PGF_(2α)含量的影响。结果离心前室温放置时间和离心后室温放置时间为0.5 h和2 h时对PGE_2和PGF_(2α)含量无显著影响。4℃临时存放时间为0.5,8,24 h时,PGF_(2α)/PGE_2的值分别为0.899,0.996,1.069,而当4℃临时存放时间达48 h时,该比值为1.337,显著升高(P<0.05)。4℃临时存放时间对PGF_(2α)含量的影响大于PGE_2。结论血样采集后应尽快离心分离血清,之后在4℃临时存放时间不应超过24 h。在实际定量工作中要对血清样品的放置条件和时间加以控制,以提高分析的准确性。
Objective To investigate the effect of placement time of clinical serum samples under different placement conditions on the detection results.Methods A female postgraduate female healthy volunteer was randomly selected for collecting 50 ml of blood,and the blood was divided into 16 groups.The blood was respectively placed at room temperature for 0.5 h and 2 h before and after centrifugation,and then temporarily stored at 4℃for 0.5,8,24,48 h after centrifugation.The effect of placement conditions on PGE_2and PGF_(2α)levels in serum was examined.Results There was no significant effect on the PGE_2and PGF_(2α)content at room temperature before centrifugation and at room temperature for 0.5 h and 2 h after centrifugation.The PGF_(2α)/PGE_2values were 0.899,0.996,and1.069 when the temporary storage time was 0.5 h,8 h and 24 h,and the ratio was 1.373 when the temporary storage time was 48 h at4℃,which was significantly higher(P<0.05).The temporary storage time at 4℃had a greater effect on PGF_(2α)content than PGE_2.Conclusion The serum should be centrifuged as soon as possible after collecting the blood sample.The temporary storage time at 4℃should not exceed 24 h.In the actual quantitative work,the placement conditions and time of the serum samples should be controlled to improve the accuracy of the analysis.
Objective To investigate the effect of placement time of clinical serum samples under different placement conditions on the detection results.Methods A female postgraduate female healthy volunteer was randomly selected for collecting 50 ml of blood,and the blood was divided into 16 groups.The blood was respectively placed at room temperature for 0.5 h and 2 h before and after centrifugation,and then temporarily stored at 4℃for 0.5,8,24,48 h after centrifugation.The effect of placement conditions on PGE_2and PGF_(2α)levels in serum was examined.Results There was no significant effect on the PGE_2and PGF_(2α)content at room temperature before centrifugation and at room temperature for 0.5 h and 2 h after centrifugation.The PGF_(2α)/PGE_2values were 0.899,0.996,and1.069 when the temporary storage time was 0.5 h,8 h and 24 h,and the ratio was 1.373 when the temporary storage time was 48 h at4℃,which was significantly higher(P<0.05).The temporary storage time at 4℃had a greater effect on PGF_(2α)content than PGE_2.Conclusion The serum should be centrifuged as soon as possible after collecting the blood sample.The temporary storage time at 4℃should not exceed 24 h.In the actual quantitative work,the placement conditions and time of the serum samples should be controlled to improve the accuracy of the analysis.
引文
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[12] Wang Y,Armando AM,Quehenberger O,et al. Comprehensive ultra-performance liquid chromatographic separation and mass spectrometric analysis of eicosanoid metabolites in human samples[J]. Chromatogr A,2014,1359:60-69.
[2]孙玉阳,纪宏宇,陈博,等.原发性痛经的发病机制及中医药治疗的研究进展[J].中国药师,2017,20(1):144-147.
[3]杨继红,陈英,胡佳贞.中学女生外周血前列腺素与原发性痛经关系[J].中国公共卫生,2011,27(8):1042-1043.
[4] Deems R,Buczynski MW,Bowersgentry R,et al. Detection and quantitation of eicosanoids via high performance liquid chromatography-electrospray ionization-mass spectrometry[J]. Methods Enzymol,2007,432:59-82.
[5] Masoodi M,Eiden M,Koulman A,et al. Comprehensive lipidomics analysis of bioactive lipids in complex regulatory networks[J]. Anal Chem,2010,82(19):8176-8185.
[6]黄群,林辉,王萍,等.痛经贴合血府逐瘀颗粒对原发性痛经寒凝血瘀证患者血清PGE2、PGF2α的影响[J].中医药导报,2018,25(1):99-101.
[7]嵇波,任晓暄,赵雅芳,等.原发性痛经发病机制与防治研究述评[J].中国医学现代杂志,2008,18(13):1856-1858.
[8]张福云,王文华,陈琳,等.暖宫七味散对原发性痛经寒凝血滞证患者血清PGF2α、PGE2水平及子宫微循环的影响[J].中国现代医生,2017,55(23):10-12.
[9] Dennis EA,Cao J,Hsu YH,et al. Phospholipase A2 enzymes:physical structure, biological function, disease implication,chemical inhibition,and therapeutic intervention[J]. Chem Rev,2011,111(10):6130-6185.
[10] Jain S,Chakraborty G,Raja R,et al. Prostaglandin E2 regulates tumor angiogenesis in prostate cancer[J]. Cancer Res,2008,68(19):7750.
[11] Smith WL,Langenbach R. Why there are two cyclooxygenase isozymes.[J]. Clin Invest,2001,107(12):1491-1495.
[12] Wang Y,Armando AM,Quehenberger O,et al. Comprehensive ultra-performance liquid chromatographic separation and mass spectrometric analysis of eicosanoid metabolites in human samples[J]. Chromatogr A,2014,1359:60-69.