钙对贮藏期马铃薯块茎渗透调节物质及SOD的影响
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摘要
【目的】本文研究了钙对贮藏期马铃薯块茎渗透调节物质及SOD的影响。【方法】采用随机区组设计,喷施Ca(NO_3)_2设置6个钙肥施用水平。在马铃薯贮藏期间,测定马铃薯块茎中几种透调节物质含量及超氧化物歧化酶活性。【结果】马铃薯经田间喷施Ca(NO_3)_2后,块茎内可溶性糖、脯氨酸等物质的含量显著增加;显著抑制MDA累积量;SOD保护酶的活性显著提高。综合分析本试验研究结果,提出Ca(NO_3)_2喷施的最佳浓度为0.6 kg/hm~2。【结论】钙能有效提高细胞渗透调节能力和原生质保护能力,从而提高了马铃薯块茎的耐贮性能。
【Objective】This paper aimed to study the effects of calcium on osmotic regulators and SOD in potato tubers during storage. 【Method】 A randomized block design was applied to spray Ca(NO_3)_2 to set 6 levels of calcium fertilizer application. The content of several permeability regulators and the activity of superoxide dismutase(SOD) in potato tubers were determined during storage. 【Result】After spraying Ca(NO_3)_2 in the field, the contents of soluble sugar, proline and other substances in potato tubers were significantly increased, the activity of SOD protective enzymes was significantly increased, and the accumulation of MDA was significantly inhibited. 【Conclusion】The comprehensive analysis of the results in this study indicated that the optimum concentration of Ca(NO_3)_2 was 0.6 kg/hm~2. Calcium could effectively improve cell osmotic regulation and protoplast protection, thus improving the storage resistance of potato tubers.
【Objective】This paper aimed to study the effects of calcium on osmotic regulators and SOD in potato tubers during storage. 【Method】 A randomized block design was applied to spray Ca(NO_3)_2 to set 6 levels of calcium fertilizer application. The content of several permeability regulators and the activity of superoxide dismutase(SOD) in potato tubers were determined during storage. 【Result】After spraying Ca(NO_3)_2 in the field, the contents of soluble sugar, proline and other substances in potato tubers were significantly increased, the activity of SOD protective enzymes was significantly increased, and the accumulation of MDA was significantly inhibited. 【Conclusion】The comprehensive analysis of the results in this study indicated that the optimum concentration of Ca(NO_3)_2 was 0.6 kg/hm~2. Calcium could effectively improve cell osmotic regulation and protoplast protection, thus improving the storage resistance of potato tubers.
引文
[1]Cother E J,Cullis B R.The influence of tuber position on periderm calcium content and its relationship to soft rot susceptibility[J].Potato Research,1992,35(3):271-277.
[2]Kratzke M G,Plata J P.Evidence for the existence of funetional roots on potato tubers and stolons:significance in water transport to the tuber.American Potato Journal,1985,62(5):227-236.
[3]Karlsson B H,Palta J P,Crump P M.Enhancing tuber calcium concentration may reduce incidence of blackspot bruise injury in potatoes[J].Hortscience A Publication of the American Society for Horticultural Science,2006,41(5):1213-1221.
[4]邢金铭,樊廷玉.镧.铈和钙对马铃薯苗株超微结构的影响[J].华北农学报,1990(1):17-21.
[5]辛建华.钙素对马铃薯生长发育、光合作用及物质代谢影响的研究[D].沈阳:沈阳农业大学,2008.
[6]刘喜平,陈彦云.钾钙元素对脱毒马铃薯费乌瑞它产量的影响[J].农业工程技术(农产品加工业),2011(11):36-37.
[7]丁红瑾.钙处理对贮藏期马铃薯主要成分及生理生化指标的影响[D].银川:宁夏大学,2013.
[8]吴玉鹏,赵晓梅,叶凯,等.钙和1-MCP处理对库尔勒香梨贮藏品质和萼端黑斑病的影响[J].新疆农业科学,2016,53(1):9-15.
[9]和银霞,李卫东,叶丽琴,等.采前喷钙对贮藏期欧李果实腐烂率及糖酸含量的影响[J].食品科学,2016,37(14):247-252.
[10]周慧娟,叶正文,苏明申,等.采前不同浓度钙喷施对‘沪油’018贮藏品质的影响[J].经济林研究,2014(3):123-128.
[11]吴友根,蒋侬辉,陈金印.钙与果品贮藏关系的研究进展[J].江西农业大学学报,2016,23(3).
[12]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:144-147.
[13]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:167-260.
[14]尹秀华,刘婷,古碧,等.木薯贮藏期呼吸强度及其主要品质变化[J].轻工科技,2011,27(4):68-69.
[15]艾玉春,王炜,李鹏霞,等.甘薯在贮藏期间营养与生理变化研究[J].食品科学技术学报,2013,31(2):37-42.
[16]Burton W G.The sugar balance in some British potato varieties during storage.I.Preliminary observations[J].European Potato Journal,1965,8(2):80-91.
[17]王秋明,朱璇,曹建康,等.负压渗钙处理对芒果贮藏品质的影响[J].食品工业科技,2007,28(3):200-201.
[18]周咏梅,谢太理,林玲,等.钙处理对巨峰葡萄夏果日灼病发生率及品质的影响[J].南方农业学报,2014,45(5):754-757.
[19]魏翠果,陈有君,蒙美莲,等.钙对 NaCl 胁迫下马铃薯脱毒苗碳水化合物代谢的影响[J].西北植物学报,2014,34(4):753-760.
[20]赵喜亭,孙颖,何男,等.CaCl2处理对铁棍山药块茎采后品质的影响[J].河南农业科学,2006,35(12):69-72.
[21]赵敏,秦舒浩,张俊莲,等.贮藏温度对连作马铃薯品质的影响[J].干旱地区农业研究,2018,36(2):49-54,160.
[22]何为华,王勤,张世英,等.套袋、喷钙对酥梨果实矿质营养和品质的影响[J].果树学报,2003,20(1):18-21.
[23]龚明,刘友良,朱培仁.低温下稻苗叶片中蛋白质及游离氨基酸的变化[J].植物生理学报,1989(4):18-22.
[24]张秀梅.热和钙处理对桃果实生理效应的研究[D].太谷:山西农业大学,2003.
[25]王爱国,罗广华.植物的超氧物自由基与羟胺反应的定量关系[J].植物生理学报,1990(6):55-57.
[26]刘萍,范琪琪,丁义峰,等.CaCl2+6-BA对芍药花瓣生理生化特性的影响[J].西南农业学报,2018(1):74-77.
[27]洪森荣,尹明华.氯化钙对野葛块根采后低温贮藏的影响[J].安徽农业科学,2007,35(10):3049-3049.
[28]邹亚丽,王廷璞,呼丽萍,等.喷施不同钙肥对苹果贮藏期间抗氧化酶活性的影响[J].干旱地区农业研究,2013,31(2):186-190.
[29]李文霞,张昕,石瑛,等.钙对马铃薯形态、生理、产量与品质性状的影响[J].东北农业大学学报,2015(7):1-8.
[30]张秀梅,杜丽清,王有年,等.钙处理对果实采后生理病害及衰老的影响[J].河北果树,2005(1):3-4.
[31]孙娅,李志强,王毓宁,等.采后钙处理对双孢菇贮藏生理的影响[J].食品工业科技,2013,34(2):322-326.
[32]胡波,李国元,谢志兵,等.钙处理对鄂北冬枣采后生理和贮藏品质的影响[J].湖北农业科学,2014(11):2634-2638.
[33]刘喜平,陈彦云,任晓月,等.钙对马铃薯块茎贮藏期间几种酶活性及蛋白质含量的影响[J].江苏农业科学,2012,40(2):62-64.
[2]Kratzke M G,Plata J P.Evidence for the existence of funetional roots on potato tubers and stolons:significance in water transport to the tuber.American Potato Journal,1985,62(5):227-236.
[3]Karlsson B H,Palta J P,Crump P M.Enhancing tuber calcium concentration may reduce incidence of blackspot bruise injury in potatoes[J].Hortscience A Publication of the American Society for Horticultural Science,2006,41(5):1213-1221.
[4]邢金铭,樊廷玉.镧.铈和钙对马铃薯苗株超微结构的影响[J].华北农学报,1990(1):17-21.
[5]辛建华.钙素对马铃薯生长发育、光合作用及物质代谢影响的研究[D].沈阳:沈阳农业大学,2008.
[6]刘喜平,陈彦云.钾钙元素对脱毒马铃薯费乌瑞它产量的影响[J].农业工程技术(农产品加工业),2011(11):36-37.
[7]丁红瑾.钙处理对贮藏期马铃薯主要成分及生理生化指标的影响[D].银川:宁夏大学,2013.
[8]吴玉鹏,赵晓梅,叶凯,等.钙和1-MCP处理对库尔勒香梨贮藏品质和萼端黑斑病的影响[J].新疆农业科学,2016,53(1):9-15.
[9]和银霞,李卫东,叶丽琴,等.采前喷钙对贮藏期欧李果实腐烂率及糖酸含量的影响[J].食品科学,2016,37(14):247-252.
[10]周慧娟,叶正文,苏明申,等.采前不同浓度钙喷施对‘沪油’018贮藏品质的影响[J].经济林研究,2014(3):123-128.
[11]吴友根,蒋侬辉,陈金印.钙与果品贮藏关系的研究进展[J].江西农业大学学报,2016,23(3).
[12]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:144-147.
[13]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:167-260.
[14]尹秀华,刘婷,古碧,等.木薯贮藏期呼吸强度及其主要品质变化[J].轻工科技,2011,27(4):68-69.
[15]艾玉春,王炜,李鹏霞,等.甘薯在贮藏期间营养与生理变化研究[J].食品科学技术学报,2013,31(2):37-42.
[16]Burton W G.The sugar balance in some British potato varieties during storage.I.Preliminary observations[J].European Potato Journal,1965,8(2):80-91.
[17]王秋明,朱璇,曹建康,等.负压渗钙处理对芒果贮藏品质的影响[J].食品工业科技,2007,28(3):200-201.
[18]周咏梅,谢太理,林玲,等.钙处理对巨峰葡萄夏果日灼病发生率及品质的影响[J].南方农业学报,2014,45(5):754-757.
[19]魏翠果,陈有君,蒙美莲,等.钙对 NaCl 胁迫下马铃薯脱毒苗碳水化合物代谢的影响[J].西北植物学报,2014,34(4):753-760.
[20]赵喜亭,孙颖,何男,等.CaCl2处理对铁棍山药块茎采后品质的影响[J].河南农业科学,2006,35(12):69-72.
[21]赵敏,秦舒浩,张俊莲,等.贮藏温度对连作马铃薯品质的影响[J].干旱地区农业研究,2018,36(2):49-54,160.
[22]何为华,王勤,张世英,等.套袋、喷钙对酥梨果实矿质营养和品质的影响[J].果树学报,2003,20(1):18-21.
[23]龚明,刘友良,朱培仁.低温下稻苗叶片中蛋白质及游离氨基酸的变化[J].植物生理学报,1989(4):18-22.
[24]张秀梅.热和钙处理对桃果实生理效应的研究[D].太谷:山西农业大学,2003.
[25]王爱国,罗广华.植物的超氧物自由基与羟胺反应的定量关系[J].植物生理学报,1990(6):55-57.
[26]刘萍,范琪琪,丁义峰,等.CaCl2+6-BA对芍药花瓣生理生化特性的影响[J].西南农业学报,2018(1):74-77.
[27]洪森荣,尹明华.氯化钙对野葛块根采后低温贮藏的影响[J].安徽农业科学,2007,35(10):3049-3049.
[28]邹亚丽,王廷璞,呼丽萍,等.喷施不同钙肥对苹果贮藏期间抗氧化酶活性的影响[J].干旱地区农业研究,2013,31(2):186-190.
[29]李文霞,张昕,石瑛,等.钙对马铃薯形态、生理、产量与品质性状的影响[J].东北农业大学学报,2015(7):1-8.
[30]张秀梅,杜丽清,王有年,等.钙处理对果实采后生理病害及衰老的影响[J].河北果树,2005(1):3-4.
[31]孙娅,李志强,王毓宁,等.采后钙处理对双孢菇贮藏生理的影响[J].食品工业科技,2013,34(2):322-326.
[32]胡波,李国元,谢志兵,等.钙处理对鄂北冬枣采后生理和贮藏品质的影响[J].湖北农业科学,2014(11):2634-2638.
[33]刘喜平,陈彦云,任晓月,等.钙对马铃薯块茎贮藏期间几种酶活性及蛋白质含量的影响[J].江苏农业科学,2012,40(2):62-64.