布渣叶多酚的提取工艺初探及其活性研究
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摘要
以乙醇为浸提溶剂,以多酚提取率为评价指标,采用加热回流方法提取布渣叶多酚,通过单因素实验和正交实验探讨了提取温度、提取时间、乙醇体积分数及料液比对布渣叶多酚提取率的影响;利用DPPH自由基、超氧阴离子自由基抗氧化检测模型和LPS诱导的RAW264.7巨噬细胞炎症模型分别评价了最优提取工艺下布渣叶多酚的抗氧化与抗炎活性。结果表明,布渣叶多酚的最优提取工艺为:料液比1∶40(g∶mL)、提取温度80℃、乙醇体积分数50%、提取时间60min,在此条件下,布渣叶多酚提取率达到(75.4±0.56)mg·g~(-1)。提取得到的布渣叶多酚具有较强的抗氧化和抗炎活性,是一种极具保健价值的天然提取物。
Using ethanol as an extraction solvent and extraction rate of polyphenols as an evaluation index,we extracted polyphenols from Microctis Folium by reflux extraction with water.Meanwhile,we investigated the influences of the extraction temperature,extraction time,ethanol volume fraction,and solid-liquid ratio on the extraction rate of Microctis Folium polyphenols by single-factor experiments and orthogonal experiments,and evaluated the antioxidant and anti-inflammatory activities of Microctis Folium polyphenols by the antioxidant detection model of DPPH and superoxide anion radicals and the inflammatory model of LPS-induced RAW264.7 macrophage.The results show that the extraction rate of Microctis Folium polyphenols reaches(75.4±0.56)mg·g~(-1) under the optimum extraction conditions as follows:the solid-liquid ratio is 1∶40(g∶mL),the extraction temperature is 80℃,the ethanol volume fraction is 50%,and the extraction time is 60 min.The Microctis Folium polyphenols are very valuable natural extracts,and have strong antioxidant and anti-inflammatory activities.
Using ethanol as an extraction solvent and extraction rate of polyphenols as an evaluation index,we extracted polyphenols from Microctis Folium by reflux extraction with water.Meanwhile,we investigated the influences of the extraction temperature,extraction time,ethanol volume fraction,and solid-liquid ratio on the extraction rate of Microctis Folium polyphenols by single-factor experiments and orthogonal experiments,and evaluated the antioxidant and anti-inflammatory activities of Microctis Folium polyphenols by the antioxidant detection model of DPPH and superoxide anion radicals and the inflammatory model of LPS-induced RAW264.7 macrophage.The results show that the extraction rate of Microctis Folium polyphenols reaches(75.4±0.56)mg·g~(-1) under the optimum extraction conditions as follows:the solid-liquid ratio is 1∶40(g∶mL),the extraction temperature is 80℃,the ethanol volume fraction is 50%,and the extraction time is 60 min.The Microctis Folium polyphenols are very valuable natural extracts,and have strong antioxidant and anti-inflammatory activities.
引文
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[2]陈迪华,常琪,斯建勇,等.天然多酚成分研究进展[J].国外医药:植物药分册,1997,12(1):9-15.CHEN D H,CHANG Q,SHI J Y,et al.Research progress on natural phenols[J].World Notes on Herbal Drugs,1997,12(1):9-15.
[3]马雯,刘玉环,阮榕生,等.膳食多酚类化合物的研究进展[J].中国酿造,2012,31(4):11-14.MA W,LIU Y H,RUAN R S,et al.Development of dietary polyphenols[J].China Brewing,2012,31(4):11-14.
[4]国家药典委员会.中华人民共和国药典(一部)[M].北京:化学工业出版社,2015:95.
[5]罗集鹏,张丽萍,杨世林,等.布渣叶的生物碱类成分研究[J].药学学报,2009(2):150-153.LUO J P,ZHANG L P,YANG S L,et al.Separation and structure elucidation of alkaloids from Chinese drug Microctis Folium[J].Acta Pharmaceutica Sinica,2009(2):150-153.
[6]孙冬梅,汪梦霞.布渣叶化学成分和药理作用研究进展[J].世界中医药,2015,10(1):143-147.SUN D M,WANG M X.Advances on chemical constituents and pharmacological effects of Microcos paniculata[J].World Chinese Medicine,2015,10(1):143-147.
[7]潘天玲,李坤平,林赞菲,等.不同产地布渣叶总黄酮含量及其清除自由基活性研究[J].广东药学院学报,2009,25(5):452-454.PAN T L,LI K P,LIN Z F,et al.Contents of total flavonoids in Microcos paniculata L.and its radical-scavenging activity[J].Journal of Guangdong College of Pharmacy,2009,25(5):452-454.
[8]潘天玲,李坤平,贲永光,等.正交试验法优化布渣叶总黄酮提取工艺的研究[J].广东药学院学报,2008,24(5):454-456.PAN T L,LI K P,BI Y G,et al.Optimization of extraction procedure of total flavonoids from Microcos paniculata L.by orthogonal test[J].Journal of Guangdong College of Pharmacy,2008,24(5):454-456.
[9]朱静平.正交设计优选油橄榄叶中多酚的提取工艺[J].食品科技,2015(5):229-232.ZHU J P.Optimization of the extraction technology of plant polyphenol from olive leaf[J].Food Science and Technology,2015(5):229-232.
[10]SINGLETON V L,ROSSI J A.Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents[J].American Journal of Enology and Viticulture,1965,16(3):144-158.
[11]ZHU K X,ZHOU H M,QIAN H F.Antioxidant and free radical-scavenging activities of wheat germ protein hydrolysates(WGPH)prepared with alcalase[J].Process Biochemistry,2006,41(6):1296-1302.
[12]唐涛,郭伟强,王珏,等.丹参酮Ⅱ-A在RAW264.7细胞系中的抗炎症作用机制[J].生物技术通讯,2007,18(1):51-53.TANG T,GUO W Q,WANG J,et al.Anti-inflammatory mechanism of the important ingredient of Salvia miltiorrhiza BungetanshinoneⅡ-A in RAW264.7cell line[J].Letters in Biotechnology,2007,18(1):51-53.
[13]谢勇,曾建伟,郑燕芳,等.基于炎症细胞模型的草珊瑚抗炎活性部位筛选[J].福建中医药大学学报,2010,20(5):35-38.XIE Y,ZENG J W,ZHENG Y F,et al.Study on anti-inflammatory active sites of Sarcandra glabra(Thunb.)Nakai produced in Fujian province[J].Journal of Fujian College of Traditional Chinese Medicine,2010,20(5):35-38.