贵州省猪圆环病毒3型的分子流行病学调查
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摘要
近年来在我国多个省份检测出新型猪圆环病毒3型(porcine circovirus 3,PCV3),为确定贵州省是否也存在该病毒,本试验对92个规模化养殖场2016—2017年所送检的308份样品采用PCR技术进行PCV3筛查,并对部分阳性样品中的ORF2进行PCR扩增、克隆及测序,将测序结果与GenBank上公布的26株ORF2序列进行遗传进化树构建、核苷酸和氨基酸比对。结果首次证实贵州省PCV3的存在,在15份PCV3阳性病料中有13份存在与其他病原共同感染现象,且多以腹泻为临床表现;株间核苷酸同源性为98.0%～100.0%,与参考毒株同源性为96.3%～99.8%,与参考株氨基酸比对相似性为97.2%～100%,遗传进化关系相对较复杂。本试验为我国PCV3的流行状况研究提供了一定的数据依据。
A total of 308 different samples were collected from 9 different areas of Guizhou province in 2016—2017,and isolated virus was identified as a porcine circovirus 3(PCV3)by PCR and sequence analysis.The PCV3 ORF2 gene fragment was first amplified in Guizhou,the homology of these ORF2 genes were from 98.0%-100.0%,shared 96.3%-99.8% nucleotide sequence identity with the corresponding sequences of PCV3 reference strains in GenBank.The similarity of the amino acid ratio of the reference strain was between 97.2%-100%.The genetic analysis showed that gene segments of the isolate were complex correlation with others PCV3.
A total of 308 different samples were collected from 9 different areas of Guizhou province in 2016—2017,and isolated virus was identified as a porcine circovirus 3(PCV3)by PCR and sequence analysis.The PCV3 ORF2 gene fragment was first amplified in Guizhou,the homology of these ORF2 genes were from 98.0%-100.0%,shared 96.3%-99.8% nucleotide sequence identity with the corresponding sequences of PCV3 reference strains in GenBank.The similarity of the amino acid ratio of the reference strain was between 97.2%-100%.The genetic analysis showed that gene segments of the isolate were complex correlation with others PCV3.
引文
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[14]湛洋,王东亮,王乃东,等.猪圆环病毒3型检测及其Cap结构序列和抗原性预测分析[J].畜牧兽医学报,2017,48(6):1076-1084.
[15]王伟丞,曾智勇,汤德元,等.猪圆环病毒1型反向遗传操作系统的初步构建[J].黑龙江畜牧兽医,2014(9):124-126.
[2]刘杏,王凤雪,温永俊,等.猪繁殖与呼吸综合征病毒和猪圆环病毒2型混合感染的流行病学调查[J].吉林农业大学学报,2016,38(4):456-459.
[3]李然,赵紫印,潘广林,等.1只秦岭地区野外捕获野猪重要猪源病毒的检测及PCV2分离株基因序列分析[J].中国兽医学报,2017,37(10):1835-1840.
[4]王伟,张双翔,金志强,等.猪流行性腹泻病毒RTLAMP检测方法的建立与应用[J].中国兽医学报,2017,37(7):1220-1224.
[5]张建武,庄金山,刘长龙,等.2007年中国部分地区猪圆环病毒2型分子流行病学分析[J].中国农业科学,2009(8):2949-2957.
[6]吴德铭,曹永长,毕英佐.猪圆环病毒研究进展[J].中国预防兽医学报,2004,26(1):79-82.
[7]许立华,芦银华,王玲,等.猪圆环病毒研究进展[J].中国病毒学,2004,19(3):82-84.
[8]夏应菊,訾占超,蔡林,等.猪细环病毒和猪圆环病毒2型混合感染状况的调查[J].畜牧兽医学报,2012,43(3):424-430.
[9]PALINSKI R,PINEYRO P,SHANG P,et al.A novel porcine circovirus distantly related to known circoviruses is associated with porcine dermatitis and nephropathy syndrome and reproductive failure[J].J Virol,2016,91(1):JVI.01879-16.
[10] PHAN T G,GIANNITTI F,ROSSOW S,et al.Detection of a novel circovirus PCV3in pigs with cardiac and multi-systemic inflammation[J].Virol J,2016,13(1):184.
[11] SHEN H,LIU X,ZHANG P,et al.Genome characterization of a porcine circovirus type 3in South China[J].Transbound Emerg Dis,2018,65(1):264-266.
[12] FAN S,KU X,CHEN F,et al.Complete genome sequence of a novel porcine circovirus type 3strain,pcv3/cn/hubei-618/2016,isolated from China[J].Genome Announce,2017,5(15):31-35.
[13]贺会利,李军,潘艳,等.广西首例猪圆环病毒三型的发现及其衣壳蛋白序列分析[J].南方农业学报,2017,48(8):1499-1503.
[14]湛洋,王东亮,王乃东,等.猪圆环病毒3型检测及其Cap结构序列和抗原性预测分析[J].畜牧兽医学报,2017,48(6):1076-1084.
[15]王伟丞,曾智勇,汤德元,等.猪圆环病毒1型反向遗传操作系统的初步构建[J].黑龙江畜牧兽医,2014(9):124-126.