东北地区狐源大肠杆菌磺胺类耐药基因的检测与分析
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摘要
为了解东北地区狐源大肠杆菌对磺胺类药物耐药基因的流行情况,本实验共选取狐源大肠杆菌156株,采用PCR技术对磺胺类sulⅠ、sulⅡ和sulⅢ耐药基因进行检测。结果显示:磺胺类药物的耐药基因sulⅠ、sulⅡ和sulⅢ均有检出,分别为19. 23%(30/156)、44. 87%(70/156)、20. 51%(32/156)。并且来自不同地区的菌株对磺胺类药物的耐药性存在明显差异。结果表明,东北地区狐源大肠杆菌存在对磺胺类药物的耐药性。
To understand the prevalence of sulfonamide-resistant genes in fox-derived Escherichia coli in Northeast China,we tested for sulfonamide-resistant genes sul Ⅰ,sul Ⅱ and sul Ⅲ in 156 selected strains of fox-derived Escherichia coli using PCR. We recorded sul Ⅰ,sul Ⅱ and sul Ⅲin 19%( 30/156),45%( 70/156) and 21%( 32/156) of tested strains,respectively. The resistance to sulfonamides differed by sample area. E. coli from foxes in northeast China had developed resistance to sulfonamides.
To understand the prevalence of sulfonamide-resistant genes in fox-derived Escherichia coli in Northeast China,we tested for sulfonamide-resistant genes sul Ⅰ,sul Ⅱ and sul Ⅲ in 156 selected strains of fox-derived Escherichia coli using PCR. We recorded sul Ⅰ,sul Ⅱ and sul Ⅲin 19%( 30/156),45%( 70/156) and 21%( 32/156) of tested strains,respectively. The resistance to sulfonamides differed by sample area. E. coli from foxes in northeast China had developed resistance to sulfonamides.
引文
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[2] 何世超,JEYAKKUMAR P,RAO A S,等.磺胺类药物化学研究新进展[J].中国科学(化学),2016,46(9):823-847.
[3] SUNDE M,NORSTR?M M.The prevalence of,associations between and conjugal transfer of antibiotic resistance genes in Escherichia coli isolated from Norwegian meat and meat products[J].Journal of Antimicrobial Chemotherapy,2006,58(4):741-747.
[4] 芮萍,马增军,杨彩然,等.狐、貉、貂源致病性大肠杆菌的分离与鉴定[M]// 中国毛皮动物科学研究进展—2014年全国毛皮动物专业学术讨论会论文集.长春:吉林科学技术出版社,2014:124-128.
[5] ZHU W Y,LIU J C,YU S Y,et al.Ag loaded WO3 nanoplates for efficient photocatalytic degradation of sulfanilamide and their bactericidal effect under visible light irradiation[J].Journal of Hazardous Materials,2016,318(15):407-416.
[6] 武瑞兵,高玉敏,王鹏翔,等.牛肉源大肠杆菌的耐药性检测及相关耐药基因分布[J].中国畜牧兽医,2015,42(2):452-458.
[7] 刘保光,李胜利,杨东东,等.河南省兔源大肠杆菌氨基糖苷类、四环素类和磺胺类耐药基因检测[J].家畜生态学报,2016,37(7):60-63.
[8] DOU X H,GONG J S,HAN X G,et al.Characterization of avian pathogenic Escherichia coli isolated in eastern China[J].Gene,2016,576(1):244-248.