表面机械研磨对医用钛合金生物活性的影响
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摘要
采用表面机械研磨(SMAT)法对Ti-25Nb-3Mo-3Zr-2Sn(TLM)钛合金进行了处理,并考察了SMAT处理前后钛合金样品表面的生物仿生矿化、蛋白吸附及成骨细胞的黏附行为.表征结果显示,SMAT处理不会改变TLM钛合金样品表面的物相组成及晶粒尺寸,但会显著改变合金的表面粗糙度、拓扑结构、亲水性及表面不同化学态氧元素的含量.在模拟体液中浸泡28 d后,未经SMAT处理的TLM钛合金样品表面没有检测到新化合物的生成,而经SMAT处理后的合金样品表面已诱导出直径为1~2μm、钙磷比约为1.58的羟基磷灰石前驱物.体外实验结果表明,SMAT处理后的钛合金样品表面能从血清中吸附更大数量的蛋白,且成骨细胞在处理后的样品表面表现出更好的黏附状态.经SMAT处理后的钛合金样品表面表现出更强的生物矿化、蛋白吸附及促细胞黏附能力,这与其表面具有更大的表面粗糙度、更好的亲水性及富含更多的基础Ti—OH基团有关.
Ti-25Nb-3Mo-3Zr-2Sn( TLM) titanium alloy was treated via surface mechanical attrition treatment( SMAT) method in this paper,subsequently the biomineralization,protein adsorption and osteoblast adhesion behaviors of treated and untreated titanium alloy samples were explored. The characteristic results by means of XRD,OM,TEM,AFM,XPS,SEM,EDX and contact angle experiment revealed that SMAT process did not alter the phase composition and grain size of TLM samples,however,it would obviously alter the surface roughness,topography,hydrophilicity and oxygen content of different chemical state of TLM samples. After immersed in the simulated body fluid solutions for 28 d,no new chemical compounds were detected on the untreated surface,nevertheless,hydroxyapatite precursor with a diameter of 1—2 μm and Ca/P ratio of 1. 58 was observed on the treated surface. In vitro experimental results showed that the SMAT-treated sample could adsorb more proteins from the serum and osteoblasts exhibited much better adhesion condition on its surface.The SMAT-treated sample possessed superior biomineralization,protein adsorption and cellular adhesion-promoted capacity to the untreated sample,which was related with much larger surface roughness,better hydrophilicity and higher content of basic Ti—OH contained on the SMAT-treated surface.
Ti-25Nb-3Mo-3Zr-2Sn( TLM) titanium alloy was treated via surface mechanical attrition treatment( SMAT) method in this paper,subsequently the biomineralization,protein adsorption and osteoblast adhesion behaviors of treated and untreated titanium alloy samples were explored. The characteristic results by means of XRD,OM,TEM,AFM,XPS,SEM,EDX and contact angle experiment revealed that SMAT process did not alter the phase composition and grain size of TLM samples,however,it would obviously alter the surface roughness,topography,hydrophilicity and oxygen content of different chemical state of TLM samples. After immersed in the simulated body fluid solutions for 28 d,no new chemical compounds were detected on the untreated surface,nevertheless,hydroxyapatite precursor with a diameter of 1—2 μm and Ca/P ratio of 1. 58 was observed on the treated surface. In vitro experimental results showed that the SMAT-treated sample could adsorb more proteins from the serum and osteoblasts exhibited much better adhesion condition on its surface.The SMAT-treated sample possessed superior biomineralization,protein adsorption and cellular adhesion-promoted capacity to the untreated sample,which was related with much larger surface roughness,better hydrophilicity and higher content of basic Ti—OH contained on the SMAT-treated surface.
引文
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[4]Basalah A.,Esmaeili S.,Toyserkani E.,J.Mater.Process Tech.,2016,238,341—351
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[10]Hsu H.C.,Wu S.C.,Hsu S.K.,Liao Y.H.,Ho W.F.,Mater.Design,2016,104,205—210
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[14]Lai M.,Cai K.Y.,Hu Y.,Yang X.F.,Liu Q.,Colloids Surf.B:Biointerfaces,2012,97,211—220
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[16]Huang R.,Zhuang H.Y.,Han Y.,Mater.Sci.Eng.C,2014,35,144—152
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[19]Tan G.X.,Wang Y.J.,Zhang S.J.,Chen R.,Ning C.Y.,Liu X.J.,Chem.J.Chinese Universities,2011,32(2),402—406(谭帼馨,王迎军,张姝江,陈荣,宁成云,刘旭建.高等学校化学学报,2011,32(2),402—406)
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[21]Samih Y.,Beausir B.,Bolle B.,Grosdidier T.,Mater.Charact.,2013,83,129—138
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[25]Pham M.T.,Matz W.,Reuther H.,Surf.Coat.Technol.,2000,128-129,313—319
[26]Liu X.Y.,Chu P.K.,Ding C.X.,Mater.Sci.Eng.R:Reports,2010,70,275—302
[27]Liu X.Y.,Poon R.W.Y.,Kwok S.C.H.,Paul K.C.,Ding C.X.,Surf.Coat.Technol.,2005,191,43—48
[28]Bhakta S.A.,Evans E.,Benavidez T.E.,Garcia C.D.,Anal.Chim.Acta,2015,872,7—25
[29]Anselme K.,Davidson P.,Popa A.M.,Giazzon M.,Liley M.,Ploux L.,Acta Biomater.,2010,6,3824—3846
[30]Feng B.,Wang J.,Qu S.X.,Chen J.Y.,Zhang X.D.,Func.Mater.,2005,2(4),23—27(冯波,翁杰,屈树新,陈继镛,张兴栋.功能材料,2005,2(4),23—27)
[31]Healy K.E.,Ducheyne P.,Biomater.,1992,13,553—561
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[35]Webster T.J.,Schadler L.S.,Siegel R.W.,Bizios R.,Tissue Eng.,2001,7,291—301
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