傣药肾茶中水溶性酚酸类化学成分的研究
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摘要
目的对傣药肾茶Clerodendranthus spicatus水溶性酚酸类化学成分进行研究。方法采用大孔树脂D101、Sephadex LH-20、MCI及制备HPLC等多种色谱技术分离纯化,并根据理化性质及波谱数据鉴定其结构。结果从肾茶中分离得到16个化合物,分别鉴定为原儿茶醛(1)、原儿茶酸(2)、3,5-二羟基苯甲醛(3)、对羟基苯甲酸(4)、对羟基苯甲醛(5)、香草酸(6)、丹参素甲酯(7)、3,5-O-二甲基没食子酸(8)、helisterculins A(9)、紫草酸(10)、紫草酸甲酯(11)、紫草酸二甲酯(12)、helisterculins B(13)、二氢咖啡酸乙酯(14)、阿魏酸(15)、二氢阿魏酸(16)。结论化合物3、6~9、13~16均为首次从该属植物中分离得到。
Objective To investigate the water-soluble phenolic acids from Clerodendranthus spicatus of Dai medicine. MethodsThe chemical constituents were separated and purified by D101, Sephadex LH-20, MCI, and preparative HPLC column chromatographies. Their structures were determined by physicochemical properties and spectral data analyses. Results Sixteen constituents were isolated and elucidated as protocatechualdehyde(1), protocatechuic acid(2), 3,5-dihydroxybenzaldehyde(3), 4-hydroxybenzoic acid(4), 4-hydroxybenzaldehyde(5), vanillic acid(6), danshensu methyl ester(7), 3,5-O-dimethyl gallic acid(8), helisterculins A(9), lithospermic acid(10), methyl lithospermate(11), dimethy lithospermate(12), helisterculins B(13), ethyl dihydrocaffeate(14), ferulic acid(15), and dihydroferulic acid(16). Conclusion Compounds 3, 6—9, and 13—16 are obtained from Clerodendranthus Kudo for the first time.
Objective To investigate the water-soluble phenolic acids from Clerodendranthus spicatus of Dai medicine. MethodsThe chemical constituents were separated and purified by D101, Sephadex LH-20, MCI, and preparative HPLC column chromatographies. Their structures were determined by physicochemical properties and spectral data analyses. Results Sixteen constituents were isolated and elucidated as protocatechualdehyde(1), protocatechuic acid(2), 3,5-dihydroxybenzaldehyde(3), 4-hydroxybenzoic acid(4), 4-hydroxybenzaldehyde(5), vanillic acid(6), danshensu methyl ester(7), 3,5-O-dimethyl gallic acid(8), helisterculins A(9), lithospermic acid(10), methyl lithospermate(11), dimethy lithospermate(12), helisterculins B(13), ethyl dihydrocaffeate(14), ferulic acid(15), and dihydroferulic acid(16). Conclusion Compounds 3, 6—9, and 13—16 are obtained from Clerodendranthus Kudo for the first time.
引文
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[4]许娜,许旭东,杨峻山.猫须草的研究进展[J].中草药,2010,41(5):848-852.
[5]许浚,张铁军,龚苏晓,等.小蓟止血活性部位的化学成分研究[J].中草药,2010,41(4):542-544.
[6]赵泽青,苏艳芳,黄雄,等.莛子藨地上部分化学成分研究[J].中草药,2016,47(12):2089-2094.
[7]吴新星.广东蛇葡萄的化学成分及生物活性研究[D].北京:中国科学院大学,2014.
[8]郑俊霞,王乃利,陈海峰,等.翠云草中酚性成分的分离与鉴定[J].中国药物化学杂志,2007,17(5):302-305.
[9]杨秀伟,韩美华,靳彦平.金线莲化学成分的研究[J].中药材,2007,30(7):797-780.
[10]Christophoridou S,Dais P,Tseng L H,et al.Separation and identification of phenolic compounds in olive oil by coupling high-performance liquid chromatography with postcolumn solid-phase extraction to nuclear magnetic resonance spectroscopy(LC-SPE-NMR)[J].J Agric Food Chem,2005,53(12):4667-4679.
[11]史社坡,蒋丹,董彩霞,等.东北铁线莲化学成分研究[J].中草药,2007,38(3):335-337.
[12]毛水春,顾谦群,崔承彬,等.中药大血藤中酚类化学成分及其抗肿瘤活性[J].中国药物化学杂志,2004,14(6):326-329.
[13]Tezuka Y,Terazono M,Kusumoto T I,et al.Helisterculins A and B,two new(7.5′,8.2′)-neoligans,and Helisorin,the first(6.4,7.5,8.2)-neolignan,from the Indonesian medicinal plant Helicteres isora[J].Helv Chim Acta,1999,82(3):408-417.
[14]刘生生,韩飞,许卉,等.丹酚酸B热解产物化学成分的分离与鉴定[J].中草药,2011,42(2):247-250.
[15]王敏,梁敬钰,陈雪英.肾茶的水溶性成分(英文)[J].中国天然药物,2007,5(1):27-30.
[16]高俊峰,丁玲,张鹏,等.石见穿化学成分研究[J].中国中药杂志,2013,38(10):1556-1559.
[17]李礼,张国刚,潘春媛,等.火绒草化学成分的研究[J].中国药物化学杂志,2009,19(3):212-213.
[18]孙博航,吉川雅之,陈英杰,等.银柴胡的化学成分[J].沈阳药科大学学报,2006,23(2):84-87.