lncFOXC1表达增加促进肝癌发生
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摘要
目的:探讨长链非编码RNA lncFOXC1在肝癌组织中的表达以及在肝癌干细胞生长中的作用。方法:收集34例手术切除的肝癌组织和癌旁组织样本,实时荧光定量PCR检测lncFOXC1的表达。同时检测lncFOXC1在肝癌干细胞小球中的表达。建立lncFOXC1低表达的稳定细胞系,通过干细胞小球形成和小鼠皮下成瘤实验观察lncFOXC1对肿瘤形成的作用。结果:lncFOXC1在肝癌组织和干细胞小球中显著高表达(P <0. 05)。lncFOXC1敲减后显著抑制了肝癌干细胞小球形成和小鼠皮下肿瘤的生长。结论:lncFOXC1在肝癌组织中高表达,调控肝癌干细胞生长。
AIM:To investigate the expression of long noncoding RNA lncFOXC1 in liver cancer and the role in liver cancer formation.METHODS:A total of 34 liver cancer and peri-tumor tissues were collected.Expression of lncFOXC1 was detected by qRT-PCR in liver cancer and peri-tumor tissues,as well as liver cancer cell spheres.LncFOXC1 deficiency cell lines were constructed by shRNA.Then,its roles in sphere formation and in vivo tumor growth assays were studied.RESULTS:lncFOXC1 was significantly highly expressed in liver cancer tissues and liver cancer cell sphere.LncFOXC1 deficiency significantly reduced sphere formation and tumor growth.CONCLUSION:LncFOXC1 is highly expressed in liver cancer tissues,and can regulate liver cancer stem cell growth.
AIM:To investigate the expression of long noncoding RNA lncFOXC1 in liver cancer and the role in liver cancer formation.METHODS:A total of 34 liver cancer and peri-tumor tissues were collected.Expression of lncFOXC1 was detected by qRT-PCR in liver cancer and peri-tumor tissues,as well as liver cancer cell spheres.LncFOXC1 deficiency cell lines were constructed by shRNA.Then,its roles in sphere formation and in vivo tumor growth assays were studied.RESULTS:lncFOXC1 was significantly highly expressed in liver cancer tissues and liver cancer cell sphere.LncFOXC1 deficiency significantly reduced sphere formation and tumor growth.CONCLUSION:LncFOXC1 is highly expressed in liver cancer tissues,and can regulate liver cancer stem cell growth.
引文
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[3]Easwaran H,Tsai HC,Baylin SB,et al.Cancer epigenetics:tumor heterogeneity,plasticity of stem-like states,and drug resistance[J].Mol Cell,2014,54(5):716-727.
[4]Kurtova AV,Xiao J,Mo Q,et al.Blocking PGE2-induced tumour repopulation abrogates bladder cancer chemoresistance[J].Nature,2015,517(7533):209-213.
[5]Takebe N,Harris PJ,Warren RQ,et al.Targeting cancer stem cells by inhibiting Wnt,Notch,and Hedgehog pathways[J].Nat Rev Clin Oncol,2010,1(2):97.
[6]Gagan J,Van Allen EM.Next-generation sequencing to guide cancer therapy[J].Gen Med,2015,7(1):80.
[7]Batista PJ,Chang HY.Long noncoding RNAs:Cellular address codes in development and disease[J].Cell,2013,152(6):1298-1307.
[8]Patil DP,Chen CK,Pickering BF,et al.m(6)ARNA methylation promotes XIST-mediated transcriptional repression[J].Nature,2016,537(7620):369.
[9]Leucci E,Vendramin R,Spinazzi M,et al.Melanoma addiction to the long non-coding RNA SAMMSON[J].Nature,2016,531(7595):518-522.
[10]Huarte M.RNA.A lncRNA links genomic variation with celiac disease[J].Science,2016,352(6281):43.
[11]Schmitt AM,Chang HY.Long noncoding RNAs in cancer pathways[J].Cancer Cell,2016,29(4):452-463.
[12]Wang Y,Lei H,Ying D,et al.The long noncoding RNA lnc TCF7 promotes self-renewal of human liver cancer stem cells through activation of wnt signaling[J].Cell Stem Cell,2015,16(4):413-425.
[13]刘永存,伍丽萍.STKⅡ在肝癌组织中的表达水平分析及临床意义[J].空军医学杂志,2016,32(5):319-322.
[14]Seok JY,Na DC,Woo HG,et al.A fibrous stromal component in hepatocellular carcinoma reveals a cholangiocarcinoma-like gene expression trait and epithelial-mesenchymal transition[J].Hepatology,2012,55(6):1776-1786.
[15]Engreitz JM,Ollikainen N,Guttman M.Long non-coding RNAs:spatial amplifiers that control nuclear structure and gene expression[J].Nat Rev Mol Cell Biol,2016,17(12):756-770.
[16]Guttman M,Donaghey J,Carey BW,et al.lincRNAs act in the circuitry controlling pluripotency and differentiation[J].Nature,2011,477(7364):295.
[17]Mchugh CA,Chen CK,Chow A,et al.The Xist lncRNA directly interacts with SHARP to silence transcription through HDAC3[J].Nature,2015,521(7551):232-236.
[18]Chu HP,Cifuentes-Rojas C,Kesner B,et al.TERRARNA Antagonizes ATRX and protects telomeres[J].Cell,2017,170(1):86-101.
[19]Meng L,Ward AJ,Chun S,et al.Towards a therapy for Angelman syndrome by reduction of a long noncoding RNA[J].Nature,2015,518(7539):409-412.
[20]李中文,吴涛,付应霄,等.MicroRNA-31在肝癌中的表达和作用[J].中国临床药理学与治疗学,2015,20(10):1098-1101.
[21]张颖,周媛凤,胡敏.肝癌侵袭转移的中医药研究进展[J].中国临床药理学与治疗学,2017,22(5):594-600.