潞党参多糖对宫颈癌细胞SiHa增殖和迁移的影响
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摘要
以上党地区道地药材潞党参的多糖成分(Lu Dangshen polysaccharides,LDP)为对象,研究其对人宫颈癌细胞Si Ha的增殖及迁移的影响。通过MTT法检测LDP对Si Ha细胞增殖的影响。通过细胞黏附试验、铺展试验和划痕试验,分析LDP对Si Ha细胞迁移的影响。结果显示,LDP显著抑制了Si Ha细胞的增殖,IC50值为0.68 mg/m L;LDP明显抑制了Si Ha细胞与外基质的黏附作用,延缓了其铺展过程,并抑制了其体外迁移运动能力。LDP对人宫颈癌细胞Si Ha的增殖、黏附、铺展以及迁移运动能力具有显著的抑制效应,具有开发为肿瘤治疗的辅助药物及保健食品的价值。
The effects of polysaccharides from Lu Dangshen(Codonopsis pilosula)(LDP)on the proliferation and migration of humancervical cancer cell Si Ha was investigated. MTT method was used to determine the effects of LDP on Si Ha cell proliferation,and cell adhesionassay,cell spreading assay and cell wound scratch assay were used to analyze the effect of LDP on Si Ha cell migration. LDP significantlyrepressed Si Ha cell proliferation(IC50 = 0.68 mg/m L),and inhibited adhesion between cells and extracellular matrix,and delayed cellspreading,and then restrained Si Ha cell migration. LDP presents solid inhibition effects on the proliferation,adhesion,spreading andmigration of human cervical cancer cell Si Ha,and thus possesses potential value for developing auxiliary medicine for tumor treatment anddietary supplement.
The effects of polysaccharides from Lu Dangshen(Codonopsis pilosula)(LDP)on the proliferation and migration of humancervical cancer cell Si Ha was investigated. MTT method was used to determine the effects of LDP on Si Ha cell proliferation,and cell adhesionassay,cell spreading assay and cell wound scratch assay were used to analyze the effect of LDP on Si Ha cell migration. LDP significantlyrepressed Si Ha cell proliferation(IC50 = 0.68 mg/m L),and inhibited adhesion between cells and extracellular matrix,and delayed cellspreading,and then restrained Si Ha cell migration. LDP presents solid inhibition effects on the proliferation,adhesion,spreading andmigration of human cervical cancer cell Si Ha,and thus possesses potential value for developing auxiliary medicine for tumor treatment anddietary supplement.
引文
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[6]胡建燃,郭阳,李平.潞党参多糖的提取及其抗氧化活性分析[J].中国食品添加剂,2016,149(7):93-96.
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[14]姜艳霞,纪朋艳,朱文赫,等.海带多糖对人肝癌细胞Bel-7402增殖的抑制作用及其机制[J].食品科学,2015,36(15):179-182.
[15]鞠瑶瑶,等.响应面试验优化脆江蓠多糖提取工艺及其对肿瘤细胞的抑制作用[J].食品科学,2016,37(8):57-62.
[16]刘容旭,高辰哲,姜帆,等.五味子多糖对两种肠道肿瘤细胞抑制作用的影响[J].食品科学,2016,37(5):192-196.
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[18]陈文霞,张培,高霞,等.硒化纹党参多糖和其抗A549细胞的活性[J].中成药,2015,37(11):2408-2413.
[19]陈嘉屿,胡林海,吴红梅,等.党参多糖类对荷瘤小鼠免疫应答及抑瘤作用研究[J].中华肿瘤防治杂志,2015,22(17):1357-1362.
[20]杨丰榕,李卓敏,高建平.党参多糖分离鉴定及体外抗肿瘤活性的研究[J].时珍国医国药,2011,22(12):2876-2878.
[21]朱皓皞,蔡勇.肿瘤微浸润癌的诊断标准差异及临床意义[J].诊断病理学杂志,2016,23(4):300-303.
[22]安彩艳,包良,阿拉坦高勒.胞外酸性与肿瘤的浸润转移[J].中国生物化学与分子生物学报,2013,29(10):926-931.
[23]朱庆均,连松刚,等.罗勒多糖对HLECs中VEGFR-2/3表达的影响[J].中药新药与临床药理,2016,27(3):342-346.
[24]朱家红,徐春燕,穆欣艺,等.当归多糖联合阿糖胞苷对移植性人白血病小鼠模型肝脏的作用机制[J].中国中药杂志,2014,39(1):121-125.
[25]赵蕊,蔡亚平,陈志宝,等.马齿苋多糖对老龄荷瘤小鼠抗宫颈癌的作用[J].中国老年学杂志,2013,33:4480-4482.
[2]关琳静,连云岚,李建宽,等.潞党参HPLC特征图谱研究[J].中国中药杂志,2015,40(14):2854-2861.
[3]World Health Organization.World Cancer Report[R].Geneva:WHO,2014.
[4]王祥宇,郑燕,鲁明,等.肿瘤代谢与肿瘤转移[J].复旦学报:医学版,2016,43(1):86-93.
[5]陈博,熊茂明,孟翔凌.重视肿瘤患者的营养支持治疗[J].肿瘤,2016,36(6):705-710.
[6]胡建燃,郭阳,李平.潞党参多糖的提取及其抗氧化活性分析[J].中国食品添加剂,2016,149(7):93-96.
[7]Weitz-schmidt G,Chreng S.Cell adhesion assays[J].MethodsMol Biol,2012,757:15-30.
[8]Chigaev A,Sklar LA.Overview:assays for studying integrindependent cell adhesion[J].Methods Mol Biol,2012,757:3-14.
[9]Vega FM,Colomba A,Reymond N,et al.Rho B regulates cellmigration through altered focal adhesion dynamics[J].Open Biol,2012,2(5):120076.
[10]许春平,杨琛琛,郑坚强,等.植物叶多糖的提取和生物活性综述[J].食品研究与开发,2014,35(14):111-114.
[11]陈高敏,王顺春,王璐,等.中药植物多糖抗皮肤光老化的研究进展[J].南京中医药大学学报,2016,32(4):396-400.
[12]解玉怀,尚庆辉,古丽美娜,等.饲料添加剂植物多糖的生物学作用[J].草业科学,2016,33(3):503-511.
[13]魏帮鸿,杨志刚,郭瑞瑞.植物多糖在水产养殖中的应用[J].饲料研究,2016,(14):39-42.
[14]姜艳霞,纪朋艳,朱文赫,等.海带多糖对人肝癌细胞Bel-7402增殖的抑制作用及其机制[J].食品科学,2015,36(15):179-182.
[15]鞠瑶瑶,等.响应面试验优化脆江蓠多糖提取工艺及其对肿瘤细胞的抑制作用[J].食品科学,2016,37(8):57-62.
[16]刘容旭,高辰哲,姜帆,等.五味子多糖对两种肠道肿瘤细胞抑制作用的影响[J].食品科学,2016,37(5):192-196.
[17]王慧,王启林,田娇,等.4种地衣提取物抗氧化和抗肿瘤活性研究[J].植物科学学报,2014,32(2):181-188.
[18]陈文霞,张培,高霞,等.硒化纹党参多糖和其抗A549细胞的活性[J].中成药,2015,37(11):2408-2413.
[19]陈嘉屿,胡林海,吴红梅,等.党参多糖类对荷瘤小鼠免疫应答及抑瘤作用研究[J].中华肿瘤防治杂志,2015,22(17):1357-1362.
[20]杨丰榕,李卓敏,高建平.党参多糖分离鉴定及体外抗肿瘤活性的研究[J].时珍国医国药,2011,22(12):2876-2878.
[21]朱皓皞,蔡勇.肿瘤微浸润癌的诊断标准差异及临床意义[J].诊断病理学杂志,2016,23(4):300-303.
[22]安彩艳,包良,阿拉坦高勒.胞外酸性与肿瘤的浸润转移[J].中国生物化学与分子生物学报,2013,29(10):926-931.
[23]朱庆均,连松刚,等.罗勒多糖对HLECs中VEGFR-2/3表达的影响[J].中药新药与临床药理,2016,27(3):342-346.
[24]朱家红,徐春燕,穆欣艺,等.当归多糖联合阿糖胞苷对移植性人白血病小鼠模型肝脏的作用机制[J].中国中药杂志,2014,39(1):121-125.
[25]赵蕊,蔡亚平,陈志宝,等.马齿苋多糖对老龄荷瘤小鼠抗宫颈癌的作用[J].中国老年学杂志,2013,33:4480-4482.