潞党参根际一株产ACC脱氨酶细菌的鉴定及促生长特性
|
摘要
党参(Codonopsis pilosula(Franch.)Nannf.)是我国常见的药用植物之一,具有多种营养价值和功效,有很高的研究价值。其中,潞党参为山西长治的道地药材。本研究对从潞党参根际土分离出的一株产ACC脱氨酶细菌M01菌株进行产酶能力测定,通过形态特征、生理生化、16S rDNA序列测定与系统发育树构建,将其鉴定为Pseudomonas brassicacearum;并对其进行产生长素、产嗜铁素、溶磷能力测定,发现该菌株具有产生长素、产嗜铁素和溶磷能力。以上研究表明M01菌株具有多种促生长特性,该菌株为今后微生物肥料的开发提供了潜力菌株。
Codonopsis pilosula(Franch.) Nannf.is a common traditional medicine that has many nutritional value and efficacy. Lu Codonopsis pilosula is a medicinal herb of changzhi. In this study, we determined the production capacity of ACC deaminase, IAA, siderophore and the ability of phosphate solubilization of bacterium M01 strain isolated from the rhizosphere soil of Codonopsis pilosula. It was identified as Pseudomonas brassicacearum based on morphological, physiological-biochemical properties,16 s r DNA sequence and phylogenic tree analysis. The results showed that strain M01 has many growth promoting characteristics, so this strain can be used as one of the potential strains for the development of microbial fertilizer.
Codonopsis pilosula(Franch.) Nannf.is a common traditional medicine that has many nutritional value and efficacy. Lu Codonopsis pilosula is a medicinal herb of changzhi. In this study, we determined the production capacity of ACC deaminase, IAA, siderophore and the ability of phosphate solubilization of bacterium M01 strain isolated from the rhizosphere soil of Codonopsis pilosula. It was identified as Pseudomonas brassicacearum based on morphological, physiological-biochemical properties,16 s r DNA sequence and phylogenic tree analysis. The results showed that strain M01 has many growth promoting characteristics, so this strain can be used as one of the potential strains for the development of microbial fertilizer.
引文
[1]关琳静,连云岚,李建宽,等.潞党参HPLC特征图谱研究[J].植物生理学报,2015,40(14):2854-2859.
[2]马雪梅,吴朝峰.药用植物党参的研究进展[J].安徽农业科学.2009,37(15):6981-6983.
[3]卢燕林,张强,李彩萍,等.地道潞党参营养特性与产地土壤特征研究[J].山西农业科学,2007,35(5):42-45.
[4]龚成文,赵欣楠,冯守疆,范爱平,王万胜.配方施肥对党参生产特性的影响[J].西北农业学报,2013,22(11):130-136.
[5]韩相鹏,骆得功,黄贤芬.种植密度施肥水平药剂处理与党参产量[J].中药材,2003,26(5):12-14.
[6]Gouda,S.,et al.,Revitalization of plant growth promoting rhizobacteria for sustainable development in agriculture.Microbiol Res,2018,206:131-140.
[7]姚军朋,姚拓,王小利.ACC脱氨酶的应用研究进展与评述[J].生物技术,2010,20(2):87-91.
[8]Chen L,Dodd I C,Theobald J C,etal.The rhizobacterium Variovorax paradoxus 5C-2,containing ACC deaminase,promotes growth and development of Arabidopsis thaliana via an ethylene-dependent pathway[J].Journal of Experimental Botany,2013,64(6):1565-1573.
[9]陈坚,丁琴,王敏,等.根际ACC脱氨酶活性细菌的分离及其促生作用研究[J].中国土壤与肥料.2012(2):92-97.
[10]赵龙飞,徐亚军,常佳丽等.具ACC脱氨酶活性大豆根瘤内生菌的筛选、抗性及促生作用[J].微生物学报,2016,56(6):1009-1021.
[11]Machuca A and Milagres A.Use of CAS-agar plate modified to study the effect of different variables on the siderophore production by Aspergillus[J].Letters in Applied Microbiology,2003,36(3):177-181.
[12]Penrose D M,Glick B R.Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhi-zobacteria[J].Physiologic Plantarum,2003,118:10-15.
[13]Holt JG,Krieg NR,Sneath PH,et al.Bergey’s Manual of Determinative Bacteriology[M].9thed.Baltimore:Williams&Wilkins,1994.
[14]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001.
[15]沈萍,范秀容,李广武.微生物学实验(第三版)[M].北京:高等教育出版社,1999.
[16]韩坤,田曾元,刘珂,等.具有ACC脱氨酶活性的海滨锦葵(Kosteletzky a pentacarpos)内生细菌对小麦耐盐性的影响[J].植物生理学报,2015,51(2):212-220.
[17]鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社,2000.
[18]李娟,王文丽,卢秉林,郭天文.甘肃省河西高钙土溶磷菌筛选及其溶磷能力初步研究[J].干旱地区农业研究(Agricultural Research in the Arid Areas),2008,26(2):8-9.
[19]冯维维,武美贤,司雨婷,等.中华补血草内生与根际具ACC脱氨酶活性细菌的筛选及其生物多样性[J].微生物学报,2016,56(4):719-728.
[20]余旋.四川核桃主产区根际解磷细菌研究[D].四川农业大学博士论文,2011.
[21]刘维红,闫淑珍,杨启银,等.ACC脱氨酶活性细菌筛选及其对番茄初生苗生长的影响[J].江苏农业科学,2006年第2期.
[22]尤红,普倩,文芳,等.ACC脱氨酶菌株的分离筛选及对丹参毛状根的影响[J].浙江理工大学学报(自然科学版),2017,1673-3851.
[23]Glick BR,Karaturovic DM,Newell PC.A novel procedure for rapid isolation of plant growth-promoting pseudomonads[J].Can J Microbiol,1995,41:533-536.
[24]Levenfors J P,Eberhard T H,Levenfors J J,et al.Biological control of snow mould(Microdochium nivale)in winter cereals by Pseudomonas brassicacearum,MA250[J].Biocontrol,2008,53(4):651-665.
[25]Zhou T,Chen D,Li C,et al.Isolation and characterization of Pseudomonas brassicacearum J12 as an antagonist against Ralstonia solanacearum and identification of its antimicrobial components.[J].Microbiological Research,2012,167(7):388-394.
[26]都浩.水稻中激素和肌醇磷酸代谢相关基因功能的抗逆功能研究[D].华中农业大学博士论文,2013.
[27]Bianco C,Imperlini E,Defez R.Legumes like more IAA[J].Plant Signaling&Behavior,2009,4(8):763-765.
[2]马雪梅,吴朝峰.药用植物党参的研究进展[J].安徽农业科学.2009,37(15):6981-6983.
[3]卢燕林,张强,李彩萍,等.地道潞党参营养特性与产地土壤特征研究[J].山西农业科学,2007,35(5):42-45.
[4]龚成文,赵欣楠,冯守疆,范爱平,王万胜.配方施肥对党参生产特性的影响[J].西北农业学报,2013,22(11):130-136.
[5]韩相鹏,骆得功,黄贤芬.种植密度施肥水平药剂处理与党参产量[J].中药材,2003,26(5):12-14.
[6]Gouda,S.,et al.,Revitalization of plant growth promoting rhizobacteria for sustainable development in agriculture.Microbiol Res,2018,206:131-140.
[7]姚军朋,姚拓,王小利.ACC脱氨酶的应用研究进展与评述[J].生物技术,2010,20(2):87-91.
[8]Chen L,Dodd I C,Theobald J C,etal.The rhizobacterium Variovorax paradoxus 5C-2,containing ACC deaminase,promotes growth and development of Arabidopsis thaliana via an ethylene-dependent pathway[J].Journal of Experimental Botany,2013,64(6):1565-1573.
[9]陈坚,丁琴,王敏,等.根际ACC脱氨酶活性细菌的分离及其促生作用研究[J].中国土壤与肥料.2012(2):92-97.
[10]赵龙飞,徐亚军,常佳丽等.具ACC脱氨酶活性大豆根瘤内生菌的筛选、抗性及促生作用[J].微生物学报,2016,56(6):1009-1021.
[11]Machuca A and Milagres A.Use of CAS-agar plate modified to study the effect of different variables on the siderophore production by Aspergillus[J].Letters in Applied Microbiology,2003,36(3):177-181.
[12]Penrose D M,Glick B R.Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhi-zobacteria[J].Physiologic Plantarum,2003,118:10-15.
[13]Holt JG,Krieg NR,Sneath PH,et al.Bergey’s Manual of Determinative Bacteriology[M].9thed.Baltimore:Williams&Wilkins,1994.
[14]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001.
[15]沈萍,范秀容,李广武.微生物学实验(第三版)[M].北京:高等教育出版社,1999.
[16]韩坤,田曾元,刘珂,等.具有ACC脱氨酶活性的海滨锦葵(Kosteletzky a pentacarpos)内生细菌对小麦耐盐性的影响[J].植物生理学报,2015,51(2):212-220.
[17]鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社,2000.
[18]李娟,王文丽,卢秉林,郭天文.甘肃省河西高钙土溶磷菌筛选及其溶磷能力初步研究[J].干旱地区农业研究(Agricultural Research in the Arid Areas),2008,26(2):8-9.
[19]冯维维,武美贤,司雨婷,等.中华补血草内生与根际具ACC脱氨酶活性细菌的筛选及其生物多样性[J].微生物学报,2016,56(4):719-728.
[20]余旋.四川核桃主产区根际解磷细菌研究[D].四川农业大学博士论文,2011.
[21]刘维红,闫淑珍,杨启银,等.ACC脱氨酶活性细菌筛选及其对番茄初生苗生长的影响[J].江苏农业科学,2006年第2期.
[22]尤红,普倩,文芳,等.ACC脱氨酶菌株的分离筛选及对丹参毛状根的影响[J].浙江理工大学学报(自然科学版),2017,1673-3851.
[23]Glick BR,Karaturovic DM,Newell PC.A novel procedure for rapid isolation of plant growth-promoting pseudomonads[J].Can J Microbiol,1995,41:533-536.
[24]Levenfors J P,Eberhard T H,Levenfors J J,et al.Biological control of snow mould(Microdochium nivale)in winter cereals by Pseudomonas brassicacearum,MA250[J].Biocontrol,2008,53(4):651-665.
[25]Zhou T,Chen D,Li C,et al.Isolation and characterization of Pseudomonas brassicacearum J12 as an antagonist against Ralstonia solanacearum and identification of its antimicrobial components.[J].Microbiological Research,2012,167(7):388-394.
[26]都浩.水稻中激素和肌醇磷酸代谢相关基因功能的抗逆功能研究[D].华中农业大学博士论文,2013.
[27]Bianco C,Imperlini E,Defez R.Legumes like more IAA[J].Plant Signaling&Behavior,2009,4(8):763-765.