不同贮藏条件对五味子质量的影响
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摘要
基于多元活性成分同时测定结合多元统计分析探讨不同贮藏条件对五味子药材质量的影响。采用超快速液相色谱-三重四极杆/线性离子阱质谱(UFLC-QTRAP-MS/MS)同时测定不同贮藏条件(包装材料,贮藏温度)五味子中木脂素(五味子酯乙、五味子醇乙、五味子丙素、五味子乙素、五味子甲素、五味子酯甲、五味子醇甲、五味子酚、戈米辛D、戈米辛J、当归酰基戈米辛H)及有机酸(L-苹果酸、酒石酸、原儿茶酸、奎宁酸)共15种指标成分的含量;根据15种目标成分的含量,用灰色关联度分析和TOPSIS法对不同贮藏五味子进行综合评价。结果表明,15种化合物在一定浓度范围内均呈现良好的线性关系,相关系数均大于0.999 1;精密度、重复性和稳定性良好;平均加样回收率在96.64%～99.96%之间,RSD均小于5%。灰色关联度分析中r_i的最大差异较小为57.5%,TOPSIS法中C_i值的最大差异较大为81.3%,两种结果均显示S4、S3、S1的综合质量较好,五味子的适宜贮藏条件为以聚乙烯密封袋为外包装存放于阴凉库。所建立的方法准确、可靠,可用于五味子药材内在质量的综合评价,本研究可为五味子适宜储藏条件的优选提供基础资料。
The quality of Schisandrae Chinensis Fructus under different storage conditions was evaluated based on simultaneous determination of multiple bioactive constituents combined with multivariate statistical analysis.An analytical method based on UFLC-QTRAP-MS/MS was used for the simultaneous determination of fifteen components including lignans(schizantherin B,schisandrol B,schizandrin C,γ-schisandrin,deoxyschizandrin,schisantherin,schisandrin,schisanhenol,gomisin D,gomisin J,and angeloylgomisin H) and organic acids((S)-malic acid,D(-)-tartaric acid,protocatechuic acid,and quinic acid) in Schisandrae Chinensis Fructus under different storage conditions(various package materials and temperatures).Samples from different storage conditions were evaluated synthetically by TOPSIS and gray related degree according to the content of 15 components.The results showed that fifteen components had good linearity(r > 0.999 1),and the limits of detection were all satisfactory.The average recoveries of standard addition for the compounds were between 96.64% and 99.96%,and the relative standard deviations were less than 5%.The greatest difference of r_i in grey related degree and C_i value in TOPSIS method were 57.5% and 81.3% respectively.The results of these two methods were similar and all showed that the holistic quality of No.4,No.3 and No.1 samples was better.The optimum storage condition for Schisandrae Chinensis Fructus was storing in a cool storage warehouse with a polyethylene sealing bag.These methods were useful for the overall assessment on quality of Schisandrae Chinensis Fructus,and this study may provide the foundation and support for optimizing suitable storage conditions of Schisandrae Chinensis Fructus.
The quality of Schisandrae Chinensis Fructus under different storage conditions was evaluated based on simultaneous determination of multiple bioactive constituents combined with multivariate statistical analysis.An analytical method based on UFLC-QTRAP-MS/MS was used for the simultaneous determination of fifteen components including lignans(schizantherin B,schisandrol B,schizandrin C,γ-schisandrin,deoxyschizandrin,schisantherin,schisandrin,schisanhenol,gomisin D,gomisin J,and angeloylgomisin H) and organic acids((S)-malic acid,D(-)-tartaric acid,protocatechuic acid,and quinic acid) in Schisandrae Chinensis Fructus under different storage conditions(various package materials and temperatures).Samples from different storage conditions were evaluated synthetically by TOPSIS and gray related degree according to the content of 15 components.The results showed that fifteen components had good linearity(r > 0.999 1),and the limits of detection were all satisfactory.The average recoveries of standard addition for the compounds were between 96.64% and 99.96%,and the relative standard deviations were less than 5%.The greatest difference of r_i in grey related degree and C_i value in TOPSIS method were 57.5% and 81.3% respectively.The results of these two methods were similar and all showed that the holistic quality of No.4,No.3 and No.1 samples was better.The optimum storage condition for Schisandrae Chinensis Fructus was storing in a cool storage warehouse with a polyethylene sealing bag.These methods were useful for the overall assessment on quality of Schisandrae Chinensis Fructus,and this study may provide the foundation and support for optimizing suitable storage conditions of Schisandrae Chinensis Fructus.
引文
1 Chinese Pharmacopoeia Commission.Pharmacopoeia of the People’s Republic of China:Vol Ⅰ(中华人民共和国药典:第一部)[M].Beijing:China Medical Science Press,2015:67.
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7 Tian HZ,Xu J,Hu R,et al.Composition analysis of lignans from Schisandra chinensis(Turcz) Baill by LC-MS/MS[J].Nat Prod Res Dev(天然产物研究与开发),2012,24:32-33.
8 Wang SN,Hua YJ,Zou LS,et al.Determination of multiple active consittuents of Scrophulariae Radix with different processed products and grey relational analysis[J].J Chin Mass Spectr Soc(质谱学报),2017,38:328-341.
9 Zeper A,Li B,Ablajan K,et al.Principle and applications of a new quadrupole-linear ion trap mass spectrometer(QTRAPTM)[J].Mod Instrum(现代仪器),2004,5:9-13.
10 Wang SN,Hua YJ,Zou LS,et al.Content determination and grey relational analysis of nucleosides and nucleobases in different “sweating” processed products of Scrophulariae Radix[J].Chin J Pharm Anal(药物分析杂志),2016,36:1027-1035.
11 Chen SY,Shi JJ,Zou LS,et al.Simultaneous determination of lignans and organic acids in Schisandrae chinensis Fructus by UFLC-QTRAP-MS/MS[J].China J Chin Mater Med(中国中药杂志),2018,43:141-148.
12 Wang S.Research on storage specification of traditional Chinese medicines of being moldy with malt,lotus seeds and nutmeg as the models[D].Beijing:Peking Union Medical College(北京协和医学院),2016.
2 Hou DY,Hui RH,Li TC,et al.Research progress of Schisandra chinesis(Turcz) Baill[J].J Anshan Norm Univ(鞍山师范学院学报),2007,6:14-17.
3 Ming YB,Zhao H,Zhou L,et al.Reseach progress of Schisandra chinesis(Turcz) Bail[J].Pract Pharm Clin Remed(实用药物与临床),2007,6:365-367.
4 Li XG,Gao Q,Weng W,et al.Research progress of Fructus Schisandra Chinesis(Turcz) Bail and its pharmacological action[J].J Chin Med Mater(中药材),2005,28:156-159.
5 Meng XC,Yan BP,Yang GH,et al.Preliminary study on the effective constituent contents of Schisandra chinensis in different storage conditions[J].Chin Med J Res Prac(现代中药研究与实践),2011,25(1):25-27.
6 Bai HB,Feng ZQ,Shi XB ,et al.Simultaneous determination of seven lignans from Schisandrae chinensis Fructus by ultra-performance liquid chromatography[J].Nat Prod Res Dev(天然产物研究与开发),2017,29:605-609.
7 Tian HZ,Xu J,Hu R,et al.Composition analysis of lignans from Schisandra chinensis(Turcz) Baill by LC-MS/MS[J].Nat Prod Res Dev(天然产物研究与开发),2012,24:32-33.
8 Wang SN,Hua YJ,Zou LS,et al.Determination of multiple active consittuents of Scrophulariae Radix with different processed products and grey relational analysis[J].J Chin Mass Spectr Soc(质谱学报),2017,38:328-341.
9 Zeper A,Li B,Ablajan K,et al.Principle and applications of a new quadrupole-linear ion trap mass spectrometer(QTRAPTM)[J].Mod Instrum(现代仪器),2004,5:9-13.
10 Wang SN,Hua YJ,Zou LS,et al.Content determination and grey relational analysis of nucleosides and nucleobases in different “sweating” processed products of Scrophulariae Radix[J].Chin J Pharm Anal(药物分析杂志),2016,36:1027-1035.
11 Chen SY,Shi JJ,Zou LS,et al.Simultaneous determination of lignans and organic acids in Schisandrae chinensis Fructus by UFLC-QTRAP-MS/MS[J].China J Chin Mater Med(中国中药杂志),2018,43:141-148.
12 Wang S.Research on storage specification of traditional Chinese medicines of being moldy with malt,lotus seeds and nutmeg as the models[D].Beijing:Peking Union Medical College(北京协和医学院),2016.