火炬松初级种子园和改良种子园无性系产脂量的变异研究
|
摘要
选出高产脂的火炬松无性系,为推广高产脂火炬松人工林提供材料,为火炬松高产脂良种选育提供理论依据。以火炬松初级种子园和改良种子园无性系为研究对象,采用人工割脂的方法对种植于广东省英德市国家火炬松良种基地的初级种子园22个无性系和改良种子园68个无性系的产脂量进行测定,用SAS软件进行统计分析。火炬松初级种子园和改良种子园无性系间产脂量均存在极显著差异。火炬松初级种子园产脂量最大的是无性系P084,以总平均值+1个标准差作为选择标准,无性系P084、A257和W30为高产脂优良品系。改良种子园无性系5的产脂量最大,为4.74 kg,比总体无性系平均值大44.95%。以无性系与小区作为考察因素对产脂量进行方差分析,结果显示自然环境条件对产脂量也有显著的影响。以总平均值+1个标准差作为选择标准,无性系5、J22、外2、11、J1和H3为高产脂优良无性系,6个高产脂优良无性系平均产脂量为4.60 kg,比群体高40.96%。本研究选出的9个高产脂优良品系,通过无性繁殖方式可进行生产推广。
In order to provide materials for promoting high oleoresin production loblolly pine plantation and scientific basis for the breeding of high oleoresin production, twenty two clones from the primary seed orchard and 68 clones from the improved seed orchard in Yingde Loblolly Pine Seed Orchard, Guangdong Province, were measured by artificial cutting, and analyzed by using software SAS. High oleoresin production superior clones were selected. There were significantly different in oleoresin production of loblolly pine clones in the primary seed orchard and the improved seed orchard. In the primary seed orchard, the oleoresin production of clone P084 is the largest, and the clone P084, A257 and W30 are elite clones based on selection criteria that is the total mean plus 1 standard deviation. In the improved seed orchard, the clone 5 was selected with the largest oleoresin production for 4.74 kg, which was larger than the overall average by 44.95%. In addition, oleoresin production was also significantly affected by the environment. Six superior clones, were selected by taking the total mean plus 1 standard deviation as the selection criteria. These clones are 5、J22、Wai2、11、J1 and H3. The average oleoresin yield of superior clones was 4.60 kg, which was 40.96 % larger than the overall average. The 9 selected clones could be promote by clonal reproduction.
In order to provide materials for promoting high oleoresin production loblolly pine plantation and scientific basis for the breeding of high oleoresin production, twenty two clones from the primary seed orchard and 68 clones from the improved seed orchard in Yingde Loblolly Pine Seed Orchard, Guangdong Province, were measured by artificial cutting, and analyzed by using software SAS. High oleoresin production superior clones were selected. There were significantly different in oleoresin production of loblolly pine clones in the primary seed orchard and the improved seed orchard. In the primary seed orchard, the oleoresin production of clone P084 is the largest, and the clone P084, A257 and W30 are elite clones based on selection criteria that is the total mean plus 1 standard deviation. In the improved seed orchard, the clone 5 was selected with the largest oleoresin production for 4.74 kg, which was larger than the overall average by 44.95%. In addition, oleoresin production was also significantly affected by the environment. Six superior clones, were selected by taking the total mean plus 1 standard deviation as the selection criteria. These clones are 5、J22、Wai2、11、J1 and H3. The average oleoresin yield of superior clones was 4.60 kg, which was 40.96 % larger than the overall average. The 9 selected clones could be promote by clonal reproduction.
引文
[1]潘志刚.15年生火炬松种源试验研究初报[J].林业科学,2000,36(s1):70-79.
[2]孙光新.火炬松栽培[M].合肥:安徽科学技术出版社,1983.
[3]杨章旗.马尾松材性与产脂性状遗传改良研究[D].北京:北京林业大学,2012.
[4]杨章旗.马尾松不同年龄产脂量及松香组分分析[J].林业科学,2014(6):147-151.
[5]安宁,丁贵杰,谌红辉.林分密度及施肥对马尾松林产脂量的影响[J].中南林业科技大学学报,2010,30(9):46-50.
[6]安宁,丁贵杰,贾宏炎,等.马尾松不同径级产脂量及松脂成分差异研究[J].中南林业科技大学学报,2015,35(8):42-45.
[7]Rockwood D L.Variation in the monoterpene composition of two oleoresin systems of loblolly pine[J].Forest Science,1973,19(2):147-153.
[8]Squillace A E,Wells O O,Rockwood D L.Inheritance of monoterpene composition in cortical oleoresin of loblolly pine[J].Silvae Genetica,1980,29(3-4):141-151.
[9]吴东山.广西马尾松松脂产量及其松脂组分的遗传变异研究[D].南宁:广西大学,2014.
[10]朱秋云,黄益江.马尾松树脂道与产脂力的回归分析研究[J].福建林业科技,2002,29(4):15-16.
[11]魏永成,刘青华,周志春,等.马尾松不同产脂力无性系光合特性差异分析[J].东北林业大学学报,2015,43(2):36-41.
[12]黄帆,陈清新,周国瑾.马尾松高产脂优良家系及优树选择研究[J].湖南林业科技,2015(6):102-105.
[13]刘月蓉.高产脂马尾松遗传测定及优良材料选择和繁育技术[D].南京:南京林业大学,2007.
[14]刘月蓉.高产脂马尾松优树自由授粉子代林产脂力测定[J].福建林学院学报,2005,25(3):229-233.
[15]张支有,刘月蓉,叶功富,等.高产脂马尾松嫁接种子园的产脂力测定[J].福建林业科技,2004(4):58-61.
[16]郑元英,洪蓉.马尾松产脂力、生长量和针叶中糖、淀粉含量的节律性变化[J].福建林业科技,1995(s1):12-16.
[17]覃冀,连辉明,曾令海,等.高产脂马尾松半同胞子代20年生测定林产脂力分析[J].广东林业科技,2005,21(2):30-34.
[18]何韵,刘天颐,蔡燕灵,等.马尾松生长和产脂性状在Ssr和Estp标记不同基因型间的变异研究[J].广东林业科技,2013,29(6):21-26.
[19]黄帆,陈清新,周国瑾.马尾松高产脂优良家系及优树选择研究[J].湖南林业科技,2015(6):102-105.
[20]刘青华,周志春,范辉华,等.马尾松产脂力与生长性状的家系变异及优良家系早期选择[J].林业科学研究,2013(6):686-691.
[21]刘青华,金国庆,王晖,等.马尾松巢式交配子代产脂力、生长和木材密度遗传分析[J].林业科学研究,2014,27(6):715-720.
[22]刘月蓉,鲍晓红,陈涵,等.高产脂马尾松无性系幼林期产脂力测定[J].武夷科学,2011,27(1):121-123.
[23]连辉明,何波祥,曾令海,等.马尾松速生、优质及高产脂半同胞家系综合选择的研究[J].广东林业科技,2002(1):1-6.
[24]庄伟瑛,张玉英,邹元熹.高产脂湿地松选择和相关因子的分析[J].江西农业大学学报,2007,29(1):55-60.
[25]雷蕾.湿地松Tps基因同源克隆及其与产脂性状的关联分析[D].南昌:江西农业大学,2014.
[26]潘显强.湿地松松脂性状的遗传变异及选择[D].南昌:江西农业大学,2014.
[27]李彦杰.湿地松家系产脂性状遗传分析与评价技术研究[D].北京:中国林业科学研究院,2013.
[28]杨代贵,许业洲,胡兴宜,等.彭场林场高产脂、高产材湿地松优树选择研究[J].湖北林业科技,2005(6):1-4.
[29]黄逢龙,刘大椿,吴茂隆,等.湿地松高产脂优树选择[J].南方林业科学,2017,45(2):24-29.
[30]张建忠,沈凤强,姜景民,等.湿地松高产脂家系产脂量和生长量的遗传力估算及协方差分析[J].浙江农林大学学报,2010,27(3):367-373.
[31]李艳,吴际友,程勇,等.湿地松半同胞家系松脂产量选择研究[J].中南林业科技大学学报,2017,37(1):48-52.
[32]Roberds J H,Strom B L,Hain F P,et al.Estimates of Genetic Parameters for Oleoresin and Growth Traits in Juvenile Loblolly Pine[J].Canadian Journal of Forest Research-Revue Canadienne De Recherche Forestiere,2003,33(12):2469-2476.
[33]Ruel J J,Ayres M P,Jr P L L.Loblolly pine responds to mechanical wounding with increased resin flow[J].Canadian Journal of Forest Research,1998,28(4):596-602.
[34]黄少伟,谢维晖.实用SAS编程与林业试验数据分析[M].广州:华南理工大学出版社,2001.
[35]谢善高,李梦华,郑小贤.主要测树因子对马尾松产脂量的影响[J].安徽农业科学,2012,40(5):2733-2736.
[36]张应中,李永泉,林敏,等.高州油茶果实性状变异分析[J].经济林研究,2013,31(4):23-27.
[37]朱永安,刘海仓,刘菊花.湿地松产脂量与直径的相关关系研究[J].湖南林业科技,2001,28(2):41-43.
[38]舒文波,杨章旗.马尾松不同采脂年龄和径级产脂量变化特点研究[J].中南林业科技大学学报,2011,31(11):39-43.
[2]孙光新.火炬松栽培[M].合肥:安徽科学技术出版社,1983.
[3]杨章旗.马尾松材性与产脂性状遗传改良研究[D].北京:北京林业大学,2012.
[4]杨章旗.马尾松不同年龄产脂量及松香组分分析[J].林业科学,2014(6):147-151.
[5]安宁,丁贵杰,谌红辉.林分密度及施肥对马尾松林产脂量的影响[J].中南林业科技大学学报,2010,30(9):46-50.
[6]安宁,丁贵杰,贾宏炎,等.马尾松不同径级产脂量及松脂成分差异研究[J].中南林业科技大学学报,2015,35(8):42-45.
[7]Rockwood D L.Variation in the monoterpene composition of two oleoresin systems of loblolly pine[J].Forest Science,1973,19(2):147-153.
[8]Squillace A E,Wells O O,Rockwood D L.Inheritance of monoterpene composition in cortical oleoresin of loblolly pine[J].Silvae Genetica,1980,29(3-4):141-151.
[9]吴东山.广西马尾松松脂产量及其松脂组分的遗传变异研究[D].南宁:广西大学,2014.
[10]朱秋云,黄益江.马尾松树脂道与产脂力的回归分析研究[J].福建林业科技,2002,29(4):15-16.
[11]魏永成,刘青华,周志春,等.马尾松不同产脂力无性系光合特性差异分析[J].东北林业大学学报,2015,43(2):36-41.
[12]黄帆,陈清新,周国瑾.马尾松高产脂优良家系及优树选择研究[J].湖南林业科技,2015(6):102-105.
[13]刘月蓉.高产脂马尾松遗传测定及优良材料选择和繁育技术[D].南京:南京林业大学,2007.
[14]刘月蓉.高产脂马尾松优树自由授粉子代林产脂力测定[J].福建林学院学报,2005,25(3):229-233.
[15]张支有,刘月蓉,叶功富,等.高产脂马尾松嫁接种子园的产脂力测定[J].福建林业科技,2004(4):58-61.
[16]郑元英,洪蓉.马尾松产脂力、生长量和针叶中糖、淀粉含量的节律性变化[J].福建林业科技,1995(s1):12-16.
[17]覃冀,连辉明,曾令海,等.高产脂马尾松半同胞子代20年生测定林产脂力分析[J].广东林业科技,2005,21(2):30-34.
[18]何韵,刘天颐,蔡燕灵,等.马尾松生长和产脂性状在Ssr和Estp标记不同基因型间的变异研究[J].广东林业科技,2013,29(6):21-26.
[19]黄帆,陈清新,周国瑾.马尾松高产脂优良家系及优树选择研究[J].湖南林业科技,2015(6):102-105.
[20]刘青华,周志春,范辉华,等.马尾松产脂力与生长性状的家系变异及优良家系早期选择[J].林业科学研究,2013(6):686-691.
[21]刘青华,金国庆,王晖,等.马尾松巢式交配子代产脂力、生长和木材密度遗传分析[J].林业科学研究,2014,27(6):715-720.
[22]刘月蓉,鲍晓红,陈涵,等.高产脂马尾松无性系幼林期产脂力测定[J].武夷科学,2011,27(1):121-123.
[23]连辉明,何波祥,曾令海,等.马尾松速生、优质及高产脂半同胞家系综合选择的研究[J].广东林业科技,2002(1):1-6.
[24]庄伟瑛,张玉英,邹元熹.高产脂湿地松选择和相关因子的分析[J].江西农业大学学报,2007,29(1):55-60.
[25]雷蕾.湿地松Tps基因同源克隆及其与产脂性状的关联分析[D].南昌:江西农业大学,2014.
[26]潘显强.湿地松松脂性状的遗传变异及选择[D].南昌:江西农业大学,2014.
[27]李彦杰.湿地松家系产脂性状遗传分析与评价技术研究[D].北京:中国林业科学研究院,2013.
[28]杨代贵,许业洲,胡兴宜,等.彭场林场高产脂、高产材湿地松优树选择研究[J].湖北林业科技,2005(6):1-4.
[29]黄逢龙,刘大椿,吴茂隆,等.湿地松高产脂优树选择[J].南方林业科学,2017,45(2):24-29.
[30]张建忠,沈凤强,姜景民,等.湿地松高产脂家系产脂量和生长量的遗传力估算及协方差分析[J].浙江农林大学学报,2010,27(3):367-373.
[31]李艳,吴际友,程勇,等.湿地松半同胞家系松脂产量选择研究[J].中南林业科技大学学报,2017,37(1):48-52.
[32]Roberds J H,Strom B L,Hain F P,et al.Estimates of Genetic Parameters for Oleoresin and Growth Traits in Juvenile Loblolly Pine[J].Canadian Journal of Forest Research-Revue Canadienne De Recherche Forestiere,2003,33(12):2469-2476.
[33]Ruel J J,Ayres M P,Jr P L L.Loblolly pine responds to mechanical wounding with increased resin flow[J].Canadian Journal of Forest Research,1998,28(4):596-602.
[34]黄少伟,谢维晖.实用SAS编程与林业试验数据分析[M].广州:华南理工大学出版社,2001.
[35]谢善高,李梦华,郑小贤.主要测树因子对马尾松产脂量的影响[J].安徽农业科学,2012,40(5):2733-2736.
[36]张应中,李永泉,林敏,等.高州油茶果实性状变异分析[J].经济林研究,2013,31(4):23-27.
[37]朱永安,刘海仓,刘菊花.湿地松产脂量与直径的相关关系研究[J].湖南林业科技,2001,28(2):41-43.
[38]舒文波,杨章旗.马尾松不同采脂年龄和径级产脂量变化特点研究[J].中南林业科技大学学报,2011,31(11):39-43.