压电传感法快速检测煎炸油极性组分含量
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摘要
基于压电传感器对介质的介电特性有灵敏的频率变化响应,建立快速测定煎炸油极性组分的压电传感法。获取煎炸油的频率响应曲线,采用Boltzmann对响应曲线进行拟合,并提取可用于测定煎炸油的压电频率特征值,结合国标柱层析法建立校正集煎炸油极性组分含量与压电频率特征值之间的标定方程:Y=9 008 070-66X,r=0.993;根据标定方程,结合国标法规定的煎炸油合格标准判别阈值27%,获得压电传感法判别阈值为9 006 281 Hz。采用压电传感法对验证集和随机挑选的油样品的极性组分含量进行检测,同时采用国标法进行对照测定。结果表明,所提出的压电传感法与国标法在测定的准确性和精密度上没有显著差异,压电传感法具有快速、简便的优势,该法有望运用于油品监督的现场检测中。
A piezoelectric sensing method was proposed for rapid detection of polar component content in frying oils.The method was based on the sensitive frequency response to the dielectric changes between the electrodes. The typical frequency shift responsive curves were obtained and fitted by Boltzmann equation from which the characteristic frequency values were extracted. The calibration equation of polar component content was obtained combined with the national standard column chromatography method as follows: Y = 9 008 070-66 X,r = 0.993. According to the calibration equation, the threshold value 9 006 281 Hz was obtained to evaluate the quality of frying oils combined with the national standard column chromatography method. The polar component content of random frying oil samples were detected quickly using the piezoelectric sensing method. For comparison, the national standard method was carried out at the same time. The results showed that there are no significant differences in measuring precision between two methods. The proposed method is rapid and convenient. It is expected to on-site inspection for supervision department.
A piezoelectric sensing method was proposed for rapid detection of polar component content in frying oils.The method was based on the sensitive frequency response to the dielectric changes between the electrodes. The typical frequency shift responsive curves were obtained and fitted by Boltzmann equation from which the characteristic frequency values were extracted. The calibration equation of polar component content was obtained combined with the national standard column chromatography method as follows: Y = 9 008 070-66 X,r = 0.993. According to the calibration equation, the threshold value 9 006 281 Hz was obtained to evaluate the quality of frying oils combined with the national standard column chromatography method. The polar component content of random frying oil samples were detected quickly using the piezoelectric sensing method. For comparison, the national standard method was carried out at the same time. The results showed that there are no significant differences in measuring precision between two methods. The proposed method is rapid and convenient. It is expected to on-site inspection for supervision department.
引文
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[13]MI X W,HE F J,XIANG M Y,et al.Novel phage amplified multichannel series piezoelectric quartz crystal sensor for rapid and sensitive detection of Mycobacterium tuberculosis[J].Analytical Chemistry,2012,84(2):939-946.
[14]ZHAO J,ZHU W,HE F.Rapidly determining E.coli and P.aeruginosa by an eight channels bulk acoustic wave impedance physical biosensor[J].Sensors and Actuators B:Chemical,2005,107:271-276.
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[16]刘玉兰,王莹辉,张振山,等.电导率法快速检测煎炸油极性组分含量的研究[J].中国粮油学报,2015,30(6):53-56.
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[2]WEISBURGER J H.Comments on the history and importance of aromatic and heterocyclic amines in public health[J].Mutation Research,2002(506/507):9-20.
[3]食用植物油煎炸过程中的卫生标准:GB 7102.1-2003[S].
[4]食用植物油煎炸过程中的极性组分(PC)的测定:GB/T 5009.202-2003[S].
[5]CALDWELL J D,COOKE B S,GREER M K.High performance liquid chromatography-size exclusion chromatog raphy for rapid analysis of total polar compounds in used frying oils[J].Journal of the American Oil Chemists’Society,2011,88:1669-1674.
[6]陈秀梅,于修烛,王亚鸽,等.基于近红外光谱的煎炸油极性组分定量分析模型构建[J].食品科学,2014,35(2):238-242.
[7]SAMII-SAKET G,BOERSMA J G,ABLETT G R,et al.Single soybean seed NMR calibration for oil measurement using commercial cooking oils[J].Journal of the American Oil Chemists’Society,2011,88(11):1795-1798.
[8]GIL B,YONG J C,YOON S H.Rapid determination of polar compounds in frying fats and oils using image analysis[J].LWT-Food Science and Technology,2004,37:657-661.
[9]ZHANG J Z,BAO L L,YAO S Z,et al.A series piezoelectric quartz crystal microbial sensing technique used for biochemical oxygen demand assay in environmental monitoring[J].Microchemical Journal,1999,62(3):405-412.
[10]REN J L,HE F J,ZHANG L L.The construction and application of a new PPY-MSPQC for L-asparaginase activity assay[J].Sensors and Actuators B:Chemical,2010,145(1):272-277.
[11]FANG G,LIU G,YANG Y,et al.Quartz crystal microbalance sensor based on molecularly imprinted polymer membrane and three-dimensional Au nanoparticles@mesoporous carbon CMK-3 functional composite for ultrasensitive and specific determination of citrinin[J].Sensors and Actuators B:Chemical,2016,230:272-280.
[12]REN J,ZHOU Y B,ZHOU Y T,et al.A piezoelectric microelectrode arrays system for real-time monitoring of bacterial contamination in fresh milk[J].Food and Bioprocess Technology,2015,8(1):228-237.
[13]MI X W,HE F J,XIANG M Y,et al.Novel phage amplified multichannel series piezoelectric quartz crystal sensor for rapid and sensitive detection of Mycobacterium tuberculosis[J].Analytical Chemistry,2012,84(2):939-946.
[14]ZHAO J,ZHU W,HE F.Rapidly determining E.coli and P.aeruginosa by an eight channels bulk acoustic wave impedance physical biosensor[J].Sensors and Actuators B:Chemical,2005,107:271-276.
[15]项美玉.新型串联式压电微生物传感器的构建及应用研究[D].长沙:湖南大学,2012.
[16]刘玉兰,王莹辉,张振山,等.电导率法快速检测煎炸油极性组分含量的研究[J].中国粮油学报,2015,30(6):53-56.
[17]任佳丽.新型压电微生物传感仪及其在临床分析中的应用研究[D].长沙:湖南大学,2009.
[18]WANG R H,CAI Q Y,TONG D F,et al.Enzymatic assay of acid phosphatase and microanalysis of Cu2+and Ag+with a SAW-impedance sensor[J].Enzyme and Microbial Technology,1998,22:36-41.
[19]毕艳兰.油脂化学[M].北京:化学工业出版,2005:105.