不同脂肪源对点带石斑鱼四种免疫指标的影响
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摘要
为研究不同脂肪源饲料对点带石斑鱼免疫力的影响,通过在饲料中添加玉米油、鱼油、花生油、大豆油、葵花籽油5种不同脂肪源,用于投喂点带石斑鱼并定期检测血液中的4种免疫指标.结果表明,不同脂肪酸源对石斑鱼血液中的红细胞数量有提高作用,其中玉米油组效果最为明显,在第7、28、35、42、49天时红细胞数量均显著提高(P<0.05).超氧化物歧化酶(SOD)实验中,除了第56天时试验组均低于对照组,其他周期有试验组的SOD含量高于对照组的,且差异显著(P<0.05);而在补体C3和溶菌酶实验中,各试验组的C3和溶菌酶含量呈周期性波动,不同脂肪源饲料对石斑鱼的补体C3和溶菌酶含量的影响存在差异.试验说明在饲料中添加脂肪酸对提高石斑鱼免疫力一定的影响,但不同脂肪源的影响效果存在差异.
The effect of 5 kinds of fat feeding sources—corn oil,fish oil,peanut oil,soybean oil,sunflower oil—on the immune capability of Epinephelus coioides is studied,in order to screen the fat source which is beneficial to improve the group resistance.The results showed that the fat sources,among which corn oil has outstanding improvement on the 7 th,28 th,35 th,42 nd,49 th day (P < 0.05),raise the number of red blood cells of the test group.In the experiment of superoxide dismutase (SOD),in all cycles except for on the 56 th day,the content of SOD of the experimental groups is remarkably higher than that of the controlled groups (P < 0.05).In the experiment of the complement C3 and lysozyme,the result demonstrated the cyclical movement of the content of the complement C3 and lysozyme in the experimental groups during various test periods.However,different fat feeding source has different effect on the content of the complement C3 and lysozyme in the experimental groups.The results showed that adding fat source in feeding has certain effect of improving the immune capability of Epinephelus coioides,but different fat feeding resource has different effect.
The effect of 5 kinds of fat feeding sources—corn oil,fish oil,peanut oil,soybean oil,sunflower oil—on the immune capability of Epinephelus coioides is studied,in order to screen the fat source which is beneficial to improve the group resistance.The results showed that the fat sources,among which corn oil has outstanding improvement on the 7 th,28 th,35 th,42 nd,49 th day (P < 0.05),raise the number of red blood cells of the test group.In the experiment of superoxide dismutase (SOD),in all cycles except for on the 56 th day,the content of SOD of the experimental groups is remarkably higher than that of the controlled groups (P < 0.05).In the experiment of the complement C3 and lysozyme,the result demonstrated the cyclical movement of the content of the complement C3 and lysozyme in the experimental groups during various test periods.However,different fat feeding source has different effect on the content of the complement C3 and lysozyme in the experimental groups.The results showed that adding fat source in feeding has certain effect of improving the immune capability of Epinephelus coioides,but different fat feeding resource has different effect.
引文
[1]谢从新.鱼类学[M].北京:中国农业出版社,2010:201-287.
[2]周燕侠.石斑鱼瞄准大众化消费[J].科学养鱼,2015(1):72.
[3]杨淑专,苏文金.网箱暂养石斑鱼体表微生物的研究[J].中国微生态学杂志,1990,2(4):48-50.
[4]覃映雪,池信才,苏永全,等.网箱养殖青石斑鱼的溃疡病病原[J].水产学报,2004,29(3):297-302.
[5]陈信忠,苏亚玲,龚艳清,等.逆转录聚合酶链式反应(RT-PCR)检测5种养殖石斑鱼的神经坏死病毒[J].中国水产科学,2004,11(3):202-207.
[6]王煜恒.不同脂肪源对异育银鲫生长、体脂沉积和血液生化指标的影响[D].南京:南京农业大学,2011.
[7]刘媛,王安利,王维娜,等.不饱和脂肪酸对鱼类免疫功能的影响[J].水利渔业,2015,25(5):92-95.
[8]林浩然,刘晓春.鱼类生理学实验技术和方法[M].广州:广东高等教育出版社,2006,27-30.
[9]杨鸢劼,邴旭文,徐增洪.不饱和脂肪酸对黄鳝生长及免疫指标的影响[J].安徽农业大学学报,2008,35(2):224-228.
[10]周兴华,陈建,向枭.水生动物营养免疫学研究进展[J].兽药与饲料添加剂,2002,6(7):32-34.
[11]PASSANTINO L,AHAMURA M,CIANCIOTTS A.Fish immunology.Binding and engulfment of Candida albicans by erythrocytes of rainbow trout(Salmo gairdneri Richardson)[J].Immunopharmacology and Immunotoxicology,2002,24(4):665-678.
[12]周玉,郭文场,杨振国.鱼类血细胞的研究进展[J].动物学杂志,2001,36(6):55-57.
[13]唐威.不同脂肪源在斑点叉尾鮰饲料中应用的研究[D].长沙:湖南农业大学,2010.
[14]肖温温.饲料中脂肪与蛋白质水平对大口黑鲈生长、体组成、非特异性免疫和血液学的影响[D].上海:上海海洋大学,2012.
[15]周小秋,李洪琴,刘汉元,等.ω-3UFA和ω-6UFA对幼建鲤生产性能和免疫功能的影响[J].中国畜牧杂志,2005,41(8):27-29.
[16]LIN Y H,SHI Y S..Dietary lipid requirement of grouper,Epinephelus malabaricus,and effects on immune response[J].Aquaculture,2003,225:243-250.
[17]陈威志.以亚麻仁油和玉米油取代鱼油对军曹鱼幼鱼成长、脂肪酸代谢及免疫反应的影响[D].台北:“国立”中山大学,2008.
[18]母昌考,王春琳.鱼类必需脂肪酸营养研究现状[J].饲料工业,2003,24(6):44-46.
[2]周燕侠.石斑鱼瞄准大众化消费[J].科学养鱼,2015(1):72.
[3]杨淑专,苏文金.网箱暂养石斑鱼体表微生物的研究[J].中国微生态学杂志,1990,2(4):48-50.
[4]覃映雪,池信才,苏永全,等.网箱养殖青石斑鱼的溃疡病病原[J].水产学报,2004,29(3):297-302.
[5]陈信忠,苏亚玲,龚艳清,等.逆转录聚合酶链式反应(RT-PCR)检测5种养殖石斑鱼的神经坏死病毒[J].中国水产科学,2004,11(3):202-207.
[6]王煜恒.不同脂肪源对异育银鲫生长、体脂沉积和血液生化指标的影响[D].南京:南京农业大学,2011.
[7]刘媛,王安利,王维娜,等.不饱和脂肪酸对鱼类免疫功能的影响[J].水利渔业,2015,25(5):92-95.
[8]林浩然,刘晓春.鱼类生理学实验技术和方法[M].广州:广东高等教育出版社,2006,27-30.
[9]杨鸢劼,邴旭文,徐增洪.不饱和脂肪酸对黄鳝生长及免疫指标的影响[J].安徽农业大学学报,2008,35(2):224-228.
[10]周兴华,陈建,向枭.水生动物营养免疫学研究进展[J].兽药与饲料添加剂,2002,6(7):32-34.
[11]PASSANTINO L,AHAMURA M,CIANCIOTTS A.Fish immunology.Binding and engulfment of Candida albicans by erythrocytes of rainbow trout(Salmo gairdneri Richardson)[J].Immunopharmacology and Immunotoxicology,2002,24(4):665-678.
[12]周玉,郭文场,杨振国.鱼类血细胞的研究进展[J].动物学杂志,2001,36(6):55-57.
[13]唐威.不同脂肪源在斑点叉尾鮰饲料中应用的研究[D].长沙:湖南农业大学,2010.
[14]肖温温.饲料中脂肪与蛋白质水平对大口黑鲈生长、体组成、非特异性免疫和血液学的影响[D].上海:上海海洋大学,2012.
[15]周小秋,李洪琴,刘汉元,等.ω-3UFA和ω-6UFA对幼建鲤生产性能和免疫功能的影响[J].中国畜牧杂志,2005,41(8):27-29.
[16]LIN Y H,SHI Y S..Dietary lipid requirement of grouper,Epinephelus malabaricus,and effects on immune response[J].Aquaculture,2003,225:243-250.
[17]陈威志.以亚麻仁油和玉米油取代鱼油对军曹鱼幼鱼成长、脂肪酸代谢及免疫反应的影响[D].台北:“国立”中山大学,2008.
[18]母昌考,王春琳.鱼类必需脂肪酸营养研究现状[J].饲料工业,2003,24(6):44-46.