3种癫痫模型的制备及应用分析
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摘要
目的:探讨不同癫痫模型的制备及其在癫痫研究方面的应用。方法:选择成年雄性SD大鼠60只随机分为杏仁核慢点燃组、杏仁核快点燃组、注射匹罗卡品组,每组20只动物,观察各组大鼠的点燃率,模型大鼠癫痫发作级别及癫痫行为Racinel评分,同时观察模型大鼠癫痫发作时的脑电图。结果:成功获得各组癫痫模型动物,各组Racinel评分差异有统计学意义(P<0.05),其中慢点燃组动物持续时间较长,快点燃组出现典型癫痫行为较早,匹罗卡品组动物存活时间长。结论:与杏仁核电点燃模型相比,匹罗卡品点燃癫痫动物成功率高,癫痫行为保持时间较久,能更好的为临床所应用,但匹罗卡品所致癫痫大鼠痫性发作持续时间变异较大,这可能为临床筛药带来了一定的不便。
Objective: To investigate the preparation of different epilepsy models and its application in epilepsy research. Methods: Sixty adult male SD rats were randomly divided into amygdala kindling slow group,amygdala kindling fast group,and pilocarpine injection group with 20 rats in each. Observing seizure grade of epilepsy rats and epileptic behavior Racinel scores,and EEG of rats during seizure. Results: Various epilepsy animal models were successfully obtained,Racinel scores were significantly different among each group( P < 0. 05). In which,epilepsy of slowly burning animals continued for a longer time,quick burning group showed typical epileptic seizure behavior earlier,and the pilocarpine animals lived longer time. Conclusion: The pilocarpine injection group showed higher success rate and epilepsy behavior lasted longer,which can better serve clinical purpose. However,pilocarpine triggered epileptic seizure time varies greatly,which might cause certain inconvenience for clinical screening.
Objective: To investigate the preparation of different epilepsy models and its application in epilepsy research. Methods: Sixty adult male SD rats were randomly divided into amygdala kindling slow group,amygdala kindling fast group,and pilocarpine injection group with 20 rats in each. Observing seizure grade of epilepsy rats and epileptic behavior Racinel scores,and EEG of rats during seizure. Results: Various epilepsy animal models were successfully obtained,Racinel scores were significantly different among each group( P < 0. 05). In which,epilepsy of slowly burning animals continued for a longer time,quick burning group showed typical epileptic seizure behavior earlier,and the pilocarpine animals lived longer time. Conclusion: The pilocarpine injection group showed higher success rate and epilepsy behavior lasted longer,which can better serve clinical purpose. However,pilocarpine triggered epileptic seizure time varies greatly,which might cause certain inconvenience for clinical screening.
引文
[1]安宁,林峰,解锦鼎.托吡酯与丙戊酸钠治疗癫痫的疗效对比观察[J].临床和实验医学杂志,2011(10):1357-1359.
[2]童武华,曹伟中,王黎恩,等.控制性高血压对创伤性重症颅脑损伤神经预后的影响[J].实用医学杂志,2005(22):2513-2514.
[3]辛志成,周政,龙连圣,等.影响老年重型颅脑损伤患者预后的危险因素[J].中国实用神经疾病杂志,2009(22):15-17.
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[11]de Biase S,Gigli GL,Valente M,et al.Lacosamide for the treatment of epilepsy[J].Expert Opin Drug Metab Toxicol,2014(3):459-468.
[12]Soe NK,Lee SK.Unusual ictal foreign language automatisms in temporal lobe epilepsy[J].J Epilepsy Res,2014(2):78-81.
[13]Ambhore A,Teo SG,Omar AR,et al.Electrocardiography series importance of QT interval in clinical practice[J].Singapore Med J,2014(12):607-612.
[14]Ibekwe RC,Chidi NA,Ebele AA,et al.Co-Morbidity of attention deficit hyperactivity disorder(ADHD)and epilepsy in children seen in university of Nigeria teaching hospital enugu:prevalence,clinical and social correlates[J].Niger Postgrad Med J,2014(4):273-278.
[15]Méndez-Armenta M,Nava-Ruíz C,Juárez-Rebollar D,et al.Oxidative stress associated with neuronal apoptosis in experimental models of epilepsy[J].Oxid Med Cell Longev,2014(3):571-573.
[16]Krishnan P,Chattopadhyay A,Saha M.Periventricular nodular heterotopia,frontonasal encephalocele,corpus callosal dysgenesis and arachnoid cyst:A constellation of abnormalities in a child with epilepsy[J].J Pediatr Neurosci,2014(3):273-275.
[17]Liu TT,He ZG,Tian XB,et al.Neural mechanisms and potential treatment of epilepsy and its complications[J].Am J Transl Res,2006(6):625-630.
[18]伍国锋,洪震,高宾丽.海马电刺激对耐药性颞叶癫痫大鼠ca1区神经元钠通道电流的影响[J].中华神经科杂志,2013(8):513-518.
[19]Clifford DB,Olney JW,Maniotis A,et al.The funetional anatomy and pathology of lithium-pilocarpine and highdose piloearpine seizures[J].Neuroscience,1987(23):953-968.
[20]Curia G,Longo D,Biagini G,et al.The piloearpine model of temporal lobe epilepsy[J].Neurosci Methods,2008(172):143-157.
[2]童武华,曹伟中,王黎恩,等.控制性高血压对创伤性重症颅脑损伤神经预后的影响[J].实用医学杂志,2005(22):2513-2514.
[3]辛志成,周政,龙连圣,等.影响老年重型颅脑损伤患者预后的危险因素[J].中国实用神经疾病杂志,2009(22):15-17.
[4]张曙光,潘天鸿.颅脑损伤患者手术预后因素回归分析[J].中华神经医学杂志,2007(1):69-71.
[5]李骊,陈志茹,宋秀娥,等.中医治疗癫痫的药物类别研究[J].中医临床杂志,2010(2):56-58.
[6]Racine RJ.Modification of seizure activity by electrical stimulation after discharge threshold[J].Electroencephalogr Clin Neurephysiol,1972(32):269-279.
[7]杨杏林,范海鹰,胡晓航.中医药治疗不同类型癫痫失神发作[J].中西医结合学报,2007(5):692-694.
[8]Higgins GA,Breysse N,Undzys E,et al.The anti-epileptic drug lacosamide(Vimpat)has anxiolytic property in rodents[J].European journal of pharmacology,2009(2):41-49.
[9]向赞,许晓莉,董大翠,等.癫痫大鼠海马IL-1 B变化的研究[J].武汉大学学报:医学版,2004(6):630-648.
[10]Valente MB,Valente SM.Pediatric epilepsy:primary care treatment and health care management[J].Nurse Pract,1998(11):43-44.
[11]de Biase S,Gigli GL,Valente M,et al.Lacosamide for the treatment of epilepsy[J].Expert Opin Drug Metab Toxicol,2014(3):459-468.
[12]Soe NK,Lee SK.Unusual ictal foreign language automatisms in temporal lobe epilepsy[J].J Epilepsy Res,2014(2):78-81.
[13]Ambhore A,Teo SG,Omar AR,et al.Electrocardiography series importance of QT interval in clinical practice[J].Singapore Med J,2014(12):607-612.
[14]Ibekwe RC,Chidi NA,Ebele AA,et al.Co-Morbidity of attention deficit hyperactivity disorder(ADHD)and epilepsy in children seen in university of Nigeria teaching hospital enugu:prevalence,clinical and social correlates[J].Niger Postgrad Med J,2014(4):273-278.
[15]Méndez-Armenta M,Nava-Ruíz C,Juárez-Rebollar D,et al.Oxidative stress associated with neuronal apoptosis in experimental models of epilepsy[J].Oxid Med Cell Longev,2014(3):571-573.
[16]Krishnan P,Chattopadhyay A,Saha M.Periventricular nodular heterotopia,frontonasal encephalocele,corpus callosal dysgenesis and arachnoid cyst:A constellation of abnormalities in a child with epilepsy[J].J Pediatr Neurosci,2014(3):273-275.
[17]Liu TT,He ZG,Tian XB,et al.Neural mechanisms and potential treatment of epilepsy and its complications[J].Am J Transl Res,2006(6):625-630.
[18]伍国锋,洪震,高宾丽.海马电刺激对耐药性颞叶癫痫大鼠ca1区神经元钠通道电流的影响[J].中华神经科杂志,2013(8):513-518.
[19]Clifford DB,Olney JW,Maniotis A,et al.The funetional anatomy and pathology of lithium-pilocarpine and highdose piloearpine seizures[J].Neuroscience,1987(23):953-968.
[20]Curia G,Longo D,Biagini G,et al.The piloearpine model of temporal lobe epilepsy[J].Neurosci Methods,2008(172):143-157.