经典烘干工艺对大黄中总蒽醌含量的影响
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摘要
目的:探索用于大黄药材干燥的经典烘干工艺的最佳条件。方法:以烘干后大黄总蒽醌含量为指标,采用HPLC法测定5种蒽醌成分(芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚)的含量,采用单因素试验以及正交试验考察烘干温度、烘干时间、"发汗"时间对大黄总蒽醌含量的影响。结果:筛选的烘干工艺的最佳条件为烘干温度60℃、烘干时间15 h、"发汗"时间18 h,大黄总蒽醌的含量为41.2 mg/g。结论:在最佳烘干条件下,大黄总蒽醌含量较高,此法适用于大黄生药材的初加工。
Objective: To explore the optimum conditions of classical drying technology for Rhei Radix et Rhizoma. Methods: Taken the total anthraquinones contents in Rhei Radix et Rhizoma after drying as the index,the contents of five anthraquinone compounds( aloeemodin,rhein,emodin,chrysophanol and physcion) were determined by HPLC. The effects of drying temperature,drying time and"Fahan"( a processing method that moisture transmitted to the surface of drug by piling up) time on total anthraquinones contents of Rhei Radix et Rhizoma were investigated by single factor test and orthogonal test. Results: The optimum conditions of drying technology were as followed:the drying temperature was 60 ℃,the drying time was 15 h,the"Fahan"time was 18 h,and the contents of anthraquinones were 41.2 mg/g.Conclusion: Under the best drying conditions,the total anthraquinones contents of Rhei Radix et Rhizoma are high. This method is suitable for preliminary processing of Rhei Radix et Rhizoma.
Objective: To explore the optimum conditions of classical drying technology for Rhei Radix et Rhizoma. Methods: Taken the total anthraquinones contents in Rhei Radix et Rhizoma after drying as the index,the contents of five anthraquinone compounds( aloeemodin,rhein,emodin,chrysophanol and physcion) were determined by HPLC. The effects of drying temperature,drying time and"Fahan"( a processing method that moisture transmitted to the surface of drug by piling up) time on total anthraquinones contents of Rhei Radix et Rhizoma were investigated by single factor test and orthogonal test. Results: The optimum conditions of drying technology were as followed:the drying temperature was 60 ℃,the drying time was 15 h,the"Fahan"time was 18 h,and the contents of anthraquinones were 41.2 mg/g.Conclusion: Under the best drying conditions,the total anthraquinones contents of Rhei Radix et Rhizoma are high. This method is suitable for preliminary processing of Rhei Radix et Rhizoma.
引文
[1]詹娟娟,伍振峰,王雅琪,等.中药材及制剂干燥工艺与装备现状及问题分析[J].中国中药杂志,2015,40(23):4715-4720.
[2]桑迎迎,周国燕,王爱民,等.中药材干燥技术研究进展[J].中成药,2010,32(12):2140-2144.
[3]段金廒,宿树兰,吕洁丽,等.药材产地加工传统经验与现代科学认识[J].中国中药杂志,2009,34(24):3151-3157.
[4]刘红亮,晏仁义,邵爱娟,等.中药材“发汗”对药材质量的影响[J].中国实验方剂学杂志,2013,19(24):349-352.
[5]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:23-24.
[6]XIONG Y,CHEN L,FAN L,et al.Free Total Rhubarb Anthraquinones Protect Intestinal Injury via Regulation of the Intestinal Immune Response in a Rat Model of Severe Acute Pancreatitis[J].Front Pharmacol,2018(9):75.
[7]CAI J,RAZZAK A,HERING J,et al.Feasibility evaluation of emodin(rhubarb extract)as an inhibitor of pancreatic cancer cell proliferation in vitro[J].JPEN J Parenter Enteral Nutr,2008,32(2):190-196.
[8]LIN Y L,WU C F,HUANG Y T,et al.Effects of rhubarb on migration of rat hepatic stellate cells[J].J Gastroenterol Hepatol,2009,24(3):453-461.
[9]HEO S K,YUN H J,NOH E K,et al.Emodin and rhein inhibit LIGHT-induced monocytes migration by blocking of ROS production[J].Vascul Pharmacol,2010,53(1-2):28-37.
[10]傅兴圣,陈菲,刘训红,等.大黄化学成分与药理作用研究新进展[J].中国新药杂志,2011,20(16):1534-1538.
[11]胡会娟,刘佳,刘金玉,等.不同干燥方法对大黄9种活性成分含量的影响研究[J].中国现代中药,2016,18(7):899-902.
[12]唐文文,李国琴,宋平顺,等.大黄干燥方法研究[J].中草药,2013,44(4):424-429.
[13]宋平顺,晋小军,赵建邦,等.不同加工方法对掌叶大黄中蒽醌类和酚酸类成分的影响[J].中国现代中药,2012,14(6):46-49.
[2]桑迎迎,周国燕,王爱民,等.中药材干燥技术研究进展[J].中成药,2010,32(12):2140-2144.
[3]段金廒,宿树兰,吕洁丽,等.药材产地加工传统经验与现代科学认识[J].中国中药杂志,2009,34(24):3151-3157.
[4]刘红亮,晏仁义,邵爱娟,等.中药材“发汗”对药材质量的影响[J].中国实验方剂学杂志,2013,19(24):349-352.
[5]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:23-24.
[6]XIONG Y,CHEN L,FAN L,et al.Free Total Rhubarb Anthraquinones Protect Intestinal Injury via Regulation of the Intestinal Immune Response in a Rat Model of Severe Acute Pancreatitis[J].Front Pharmacol,2018(9):75.
[7]CAI J,RAZZAK A,HERING J,et al.Feasibility evaluation of emodin(rhubarb extract)as an inhibitor of pancreatic cancer cell proliferation in vitro[J].JPEN J Parenter Enteral Nutr,2008,32(2):190-196.
[8]LIN Y L,WU C F,HUANG Y T,et al.Effects of rhubarb on migration of rat hepatic stellate cells[J].J Gastroenterol Hepatol,2009,24(3):453-461.
[9]HEO S K,YUN H J,NOH E K,et al.Emodin and rhein inhibit LIGHT-induced monocytes migration by blocking of ROS production[J].Vascul Pharmacol,2010,53(1-2):28-37.
[10]傅兴圣,陈菲,刘训红,等.大黄化学成分与药理作用研究新进展[J].中国新药杂志,2011,20(16):1534-1538.
[11]胡会娟,刘佳,刘金玉,等.不同干燥方法对大黄9种活性成分含量的影响研究[J].中国现代中药,2016,18(7):899-902.
[12]唐文文,李国琴,宋平顺,等.大黄干燥方法研究[J].中草药,2013,44(4):424-429.
[13]宋平顺,晋小军,赵建邦,等.不同加工方法对掌叶大黄中蒽醌类和酚酸类成分的影响[J].中国现代中药,2012,14(6):46-49.