粘糯杂交后代主要农艺性状遗传变异及相关性和主成分分析
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摘要
[目的]分析粘糯杂交后代农艺性状的遗传变异,为选择植株性状与稻米品质兼优的粘糯杂交后代株系提供参考。[方法]采用相关分析和主成分分析等方法对粘糯交组合糯89-1/蜀恢527的F_2代株高、穗长、结实率、千粒重、有效穗数、每穗实粒数、每穗总粒数、着粒密度进行了研究。[结果] F_2代各农艺性状变异较大,除株高外,其他7个性状近似正态分布,株高、穗长、每穗总粒数、着粒密度表型值表现正向超亲优势;结实率与千粒重、每穗实粒数呈正相关,与株高呈负相关,千粒重与除着粒密度外的其他6个性状呈正相关;主成分分析将8个性状简化为4个主成分,即产量因子、结实率因子、有效穗数因子、千粒重因子,以上因子提供的信息量占总信息量的88.374%,能概括农艺性状的绝大部分信息。[结论]粘糯杂交后代农艺性状的遗传变异与籼粳交或品种间杂交相似。
[Objective] To analyze the genetic variation of the main agronomic traits in F_2 population of glutinous×non-glutinous, and to provide basis for selection of hybrid offspring with excellent agronomic traits and rice quality. [Method] Correlation analysis and principal component analysis were used to study the plant height, panicle length, seed setting rate, kilo-grains weight, effective panicle number, filled grain number per panicle, spikelet number per panicle and density of panicle of F_2 population of glutinous rice waxy cross 89-1/Shuhui 527. [Result] The agronomic traits varied greatly in F_2 generation. Seven traits were normal distribution, except plant height. The plant height, panicle length, spikelet number per panicle, and density of panicle phenotype showed positive super-parents. The seed setting rate was positively correlated with kilo-grains weight and filled grain number per panicle, respectively, but negatively correlated with plant height. Kilo-grains weight was positively correlated with six traits except density of panicle. The principal component analysis reduced the eight traits to four principal components, i.e. yield factor, seed setting rate factor, effective panicle number factor and kilo-grains weight factor. The above four factor provided 88.374% of the total information, which covered most information of agronomic traits. [Conclusion] The genetic variation of agronomic traits in the offspring of glutinous×non-glutinous is similar to that of indica × japonica cross or cross between varieties.
[Objective] To analyze the genetic variation of the main agronomic traits in F_2 population of glutinous×non-glutinous, and to provide basis for selection of hybrid offspring with excellent agronomic traits and rice quality. [Method] Correlation analysis and principal component analysis were used to study the plant height, panicle length, seed setting rate, kilo-grains weight, effective panicle number, filled grain number per panicle, spikelet number per panicle and density of panicle of F_2 population of glutinous rice waxy cross 89-1/Shuhui 527. [Result] The agronomic traits varied greatly in F_2 generation. Seven traits were normal distribution, except plant height. The plant height, panicle length, spikelet number per panicle, and density of panicle phenotype showed positive super-parents. The seed setting rate was positively correlated with kilo-grains weight and filled grain number per panicle, respectively, but negatively correlated with plant height. Kilo-grains weight was positively correlated with six traits except density of panicle. The principal component analysis reduced the eight traits to four principal components, i.e. yield factor, seed setting rate factor, effective panicle number factor and kilo-grains weight factor. The above four factor provided 88.374% of the total information, which covered most information of agronomic traits. [Conclusion] The genetic variation of agronomic traits in the offspring of glutinous×non-glutinous is similar to that of indica × japonica cross or cross between varieties.
引文
[1] 邓兴旺,王海洋,唐晓艳,等.杂交水稻育种将迎来新时代[J].中国科学:生命科学,2013,43(10):864-868.
[2] 程式华.中国超级稻育种技术创新与应用[J].中国农业科学,2016,49(2):205-206.
[3] 莫惠栋.我国稻米品质的改良[J].中国农业科学,1993,26(4):8-14.
[4] 程式华,闵绍楷.中国水稻品种:现状与展望[J].中国稻米,2000(1):13-16.
[5] TIAN Z X,YAN C J,QIAN Q,et al.Development of gene-tagged molecular markers for starch synthesis-related genes in rice[J].Chinese Sci Bull,2010,55(33):3768-3777.
[6] 张昌泉,赵冬生,李钱峰,等.稻米品质性状基因的克隆与功能研究进展[J].中国农业科学,2016,49(22):4267-4283.
[7] 高维维,陈思平,王丽平,等.稻米蒸煮品质性状与分子标记关联研究[J].中国农业科学,2017,50(4):599-611.
[8] 王俊仁,周凤明,吕宏飞,等.水稻杂交育种的技术优势与应用[J].上海农业科技,2012(3):23-25.
[9] 夏如兵.中国近代的水稻杂交育种研究[J].中国农学通报,2011,27(1):11-16.
[10] 陈学珍,谢皓,李莉,等.大豆杂交F2代农艺性状的遗传变异分析[J].北京农学院学报,2002,17(4):1-7.
[11] 刘文东.李树杂交后代亲子性状遗传变异规律[J].中国林副特产,2013(5):96-97.
[12] XIAO J,LI J,YUAN L,et al.Genetic diversity and its relationship to hybrid performance and heterosis in rice as revealed by PCR-based markers[J].Theoretical & applied genetics,1996,92(6):637-643.
[13] ZHAO Z W,LUO A C,LE T,et al.Identification of quantitative trait locus for overwintering germinability in rice(Oryza sativa L.)[J].Sci Agric Sin,2012,11(11):1767-1774.
[14] 赵正武,李仕贵,雷树凡.一份特异多年生水稻种质主要农艺性状的遗传变异[J].中国水稻科学,2006,20(5):481-486.
[15] 周军杰,郑剑,李兴星,等.水稻再生苗后期耐寒性生理特性研究[J].西南农业学报,2016,29(4):787-791.
[16] 王玉平,李仕贵,黎汉云,等.高配合力优质水稻恢复系蜀恢527的选育与利用[J].杂交水稻,2004,19(4):12-14.
[17] 韩龙植,魏兴华,曹桂兰,等.水稻种质资源描述规范和数据标准[M].北京:中国农业出版社,2006.
[18] 赖瑞强,李荣华,夏岩石,等.基于重组自交系群体的烟草农艺性状遗传变异分析[J].分子植物育种,2018(11):3732-3739.
[19] 李玉发,窦忠玉,梁军,等.花生主要农艺性状的遗传变异及相关性和主成分分析[J].辽宁农业科学,2013(3):11-14.
[20] PARTRIDGE M,JABRI M.Robust principal component analysis[J].Journal of the ACM,2011,58(3):11.
[21] TIPPING M E,BISHOP C M.Probabilistic principal component analysis[J].Journal of the royal statistical society,1999,61(3):611-622.
[22] 李雁,肖植文,伏成秀,等.云南28个玉米杂交组合主成分分析及综合评价[J].西南农业学报,2015,28(1):34-40.
[23] 万建民.水稻籼粳交杂种优势利用研究[J].杂交水稻,2010(S1):3-6.
[24] 宋昕蔚,林建荣,吴明国.水稻籼粳亚种间杂种优势利用研究进展与展望[J].科学通报,2016,61(35):3778-3786.
[25] 刘绍权.水稻粘糯杂交育种研究[J].种子,2005,24(2):87-88.
[26] 赵正武,王述民,李世平,等.珍稀稻种资源越冬糯稻89-1研究初报[J].杂交水稻,2000,15(3):3-4.
[27] 梁永书,李艳萍,孙海波,等.籼粳交组合培矮64S/日本晴F2、F3及F6代主要农艺性状分析[J].植物学通报,2008,25(1):59-66.
[28] 高维维,陈立凯,胡朝旭,等.基于籼粳杂交重组自交系的水稻籽粒性状的遗传变异研究[J].西北农业学报,2016,25(7):979-988.
[29] 杨川航.利用RILs群体对水稻正季和再生季重要农艺性状的QTL比较分析[D].雅安:四川农业大学,2009.
[30] 李华丽,陈美霞,阮奇城,等.烟草 F2:3家系 7 个主要产量性状的遗传变异分析[J].中国农学通报,2011,27(16):94-98.
[31] 邹玉霞,罗安才,赵正武.重庆及周边地区优异地方稻种资源遗传相关及聚类分析[J].西南农业学报,2013,26(3):884-888.
[32] 杨久,丁鲲,卢义宣,等.云南籼粳交错区水稻农艺性状与产量相关及通径分析[J].西南农业学报,2011,24(2):391-395.
[2] 程式华.中国超级稻育种技术创新与应用[J].中国农业科学,2016,49(2):205-206.
[3] 莫惠栋.我国稻米品质的改良[J].中国农业科学,1993,26(4):8-14.
[4] 程式华,闵绍楷.中国水稻品种:现状与展望[J].中国稻米,2000(1):13-16.
[5] TIAN Z X,YAN C J,QIAN Q,et al.Development of gene-tagged molecular markers for starch synthesis-related genes in rice[J].Chinese Sci Bull,2010,55(33):3768-3777.
[6] 张昌泉,赵冬生,李钱峰,等.稻米品质性状基因的克隆与功能研究进展[J].中国农业科学,2016,49(22):4267-4283.
[7] 高维维,陈思平,王丽平,等.稻米蒸煮品质性状与分子标记关联研究[J].中国农业科学,2017,50(4):599-611.
[8] 王俊仁,周凤明,吕宏飞,等.水稻杂交育种的技术优势与应用[J].上海农业科技,2012(3):23-25.
[9] 夏如兵.中国近代的水稻杂交育种研究[J].中国农学通报,2011,27(1):11-16.
[10] 陈学珍,谢皓,李莉,等.大豆杂交F2代农艺性状的遗传变异分析[J].北京农学院学报,2002,17(4):1-7.
[11] 刘文东.李树杂交后代亲子性状遗传变异规律[J].中国林副特产,2013(5):96-97.
[12] XIAO J,LI J,YUAN L,et al.Genetic diversity and its relationship to hybrid performance and heterosis in rice as revealed by PCR-based markers[J].Theoretical & applied genetics,1996,92(6):637-643.
[13] ZHAO Z W,LUO A C,LE T,et al.Identification of quantitative trait locus for overwintering germinability in rice(Oryza sativa L.)[J].Sci Agric Sin,2012,11(11):1767-1774.
[14] 赵正武,李仕贵,雷树凡.一份特异多年生水稻种质主要农艺性状的遗传变异[J].中国水稻科学,2006,20(5):481-486.
[15] 周军杰,郑剑,李兴星,等.水稻再生苗后期耐寒性生理特性研究[J].西南农业学报,2016,29(4):787-791.
[16] 王玉平,李仕贵,黎汉云,等.高配合力优质水稻恢复系蜀恢527的选育与利用[J].杂交水稻,2004,19(4):12-14.
[17] 韩龙植,魏兴华,曹桂兰,等.水稻种质资源描述规范和数据标准[M].北京:中国农业出版社,2006.
[18] 赖瑞强,李荣华,夏岩石,等.基于重组自交系群体的烟草农艺性状遗传变异分析[J].分子植物育种,2018(11):3732-3739.
[19] 李玉发,窦忠玉,梁军,等.花生主要农艺性状的遗传变异及相关性和主成分分析[J].辽宁农业科学,2013(3):11-14.
[20] PARTRIDGE M,JABRI M.Robust principal component analysis[J].Journal of the ACM,2011,58(3):11.
[21] TIPPING M E,BISHOP C M.Probabilistic principal component analysis[J].Journal of the royal statistical society,1999,61(3):611-622.
[22] 李雁,肖植文,伏成秀,等.云南28个玉米杂交组合主成分分析及综合评价[J].西南农业学报,2015,28(1):34-40.
[23] 万建民.水稻籼粳交杂种优势利用研究[J].杂交水稻,2010(S1):3-6.
[24] 宋昕蔚,林建荣,吴明国.水稻籼粳亚种间杂种优势利用研究进展与展望[J].科学通报,2016,61(35):3778-3786.
[25] 刘绍权.水稻粘糯杂交育种研究[J].种子,2005,24(2):87-88.
[26] 赵正武,王述民,李世平,等.珍稀稻种资源越冬糯稻89-1研究初报[J].杂交水稻,2000,15(3):3-4.
[27] 梁永书,李艳萍,孙海波,等.籼粳交组合培矮64S/日本晴F2、F3及F6代主要农艺性状分析[J].植物学通报,2008,25(1):59-66.
[28] 高维维,陈立凯,胡朝旭,等.基于籼粳杂交重组自交系的水稻籽粒性状的遗传变异研究[J].西北农业学报,2016,25(7):979-988.
[29] 杨川航.利用RILs群体对水稻正季和再生季重要农艺性状的QTL比较分析[D].雅安:四川农业大学,2009.
[30] 李华丽,陈美霞,阮奇城,等.烟草 F2:3家系 7 个主要产量性状的遗传变异分析[J].中国农学通报,2011,27(16):94-98.
[31] 邹玉霞,罗安才,赵正武.重庆及周边地区优异地方稻种资源遗传相关及聚类分析[J].西南农业学报,2013,26(3):884-888.
[32] 杨久,丁鲲,卢义宣,等.云南籼粳交错区水稻农艺性状与产量相关及通径分析[J].西南农业学报,2011,24(2):391-395.