氨磺乐灵对蚯蚓抗氧化酶活性及基因表达的影响
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摘要
以赤子爱胜蚓(Eisenia foetida)为实验对象,研究了不同浓度(0、2.5、5、10、15 mg·kg~(-1))的氨磺乐灵对蚯蚓体内抗氧化酶(SOD、CAT、GSH-ST)活性和相应基因表达的影响.结果表明:暴露21天后,蚯蚓体内的SOD、CAT、GSH-ST活性均随着氨磺乐灵浓度的增高而降低,其中,在2.5 mg·kg~(-1)组中,三种酶活均显著高于其他组;三种酶的基因表达均在低氨磺乐灵的组上调,而在高氨磺乐灵的组下调,三种酶的基因表达在5 mg·kg~(-1)组明显上调(p<0.01),CAT和GST在高浓度组(15 mg·kg~(-1))的表达显著下调(p<0.01和p<0.05).研究表明:蚯蚓体内的SOD、CAT和GSH-ST活性和相应基因表达对氨磺乐灵的土壤污染响应敏感,可以将其作为监测氨磺乐灵土壤生态毒性效应的生物标志物.
In the report, Eisenia foetida was used as the model to study the effects of oryzalin at different concentrations(0, 2.5, 5, 10, and 15 mg·kg~(-1)) on activities of total superoxide dismutase(T-SOD), catalase(CAT),glutathions S-transferase(GST), and the relative expressions of SOD, CAT and GST gene in earthworm. The results showed that the activities of SOD, CAT and GST were first activated and then decreased with the increase of oryzalin concentration after 21 day exposure. The activities of the three enzymes in E.foetida by exposure to oryzalin at 2.5 mg·kg~(-1) soil were significantly higher than those at the other dose levels. The relative expression levels of the three enzymes were up-regulated in the low-concentration group and down-regulated in the high-concentration group. The relative expressions of Allantioxidant enzyme gene were significantly up-regulated in E.foetida by exposure to oryzalin at 5 mg·kg~(-1) soil(p<0.01). CAT and GST gene expression were significantly down-regulated in E.foetida by exposure to oryzalin at 15 mg·kg~(-1) soil(p<0.01 and p<0.05). These results showed that the three antioxidant enzyme activities and their relative expression of genes were sensitive to the soil under the stress of oryzalin, which could be used as the potential biomarkers to monitor the ecotoxic effects of oryzalin on earthworms.
In the report, Eisenia foetida was used as the model to study the effects of oryzalin at different concentrations(0, 2.5, 5, 10, and 15 mg·kg~(-1)) on activities of total superoxide dismutase(T-SOD), catalase(CAT),glutathions S-transferase(GST), and the relative expressions of SOD, CAT and GST gene in earthworm. The results showed that the activities of SOD, CAT and GST were first activated and then decreased with the increase of oryzalin concentration after 21 day exposure. The activities of the three enzymes in E.foetida by exposure to oryzalin at 2.5 mg·kg~(-1) soil were significantly higher than those at the other dose levels. The relative expression levels of the three enzymes were up-regulated in the low-concentration group and down-regulated in the high-concentration group. The relative expressions of Allantioxidant enzyme gene were significantly up-regulated in E.foetida by exposure to oryzalin at 5 mg·kg~(-1) soil(p<0.01). CAT and GST gene expression were significantly down-regulated in E.foetida by exposure to oryzalin at 15 mg·kg~(-1) soil(p<0.01 and p<0.05). These results showed that the three antioxidant enzyme activities and their relative expression of genes were sensitive to the soil under the stress of oryzalin, which could be used as the potential biomarkers to monitor the ecotoxic effects of oryzalin on earthworms.
引文
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[3] 张建莹,岳振峰,蔡伊娜.超高效液相色谱-串联质谱法测定复杂基质食品中二硝基苯胺类除草剂残留量[J].分析测试学报,2017,36(4):550-554.
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[12]平令文,李现旭,张翠,等.DEP 对蚯蚓抗氧化酶系的影响及 DNA 损伤[J].环境科学,2018,39(10):4825-4833.
[13]曹四平,刘长海.两种生物农药对蚯蚓的急性毒性及氧化胁迫效应[J].甘肃科学学报,2017,29(5):67-72.
[14]贾茹,王梦远,黄擎.土壤辛硫磷污染对蚯蚓的生态毒理效应研究[J].环境污染与防治,2017,39(9):943-947.
[15] 冯少君,程华民,陶菁,等.全氟辛酸暴露对蚯蚓DNA 损伤和抗氧化酶基因表达水平的影响[J].热带生物学报,2016,7(2):253-258.
[16] 王晓蓉,罗义,施华宏,等.分子生物标志物在污染环境早期诊断和生态风险评价中的应用[J].环境化学,2006,25(3):320-325.
[17] 肖广侠,徐文远,贾磊,等.悬浮物对褐牙鲆抗氧化酶活性及相关基因表达的影响[J].生态毒理学报,2016,11(6):266-276.
[2] 姜锦林,单正军,周军英,等.常用农药对赤子爱胜蚓急性毒性和抗氧化酶系的影响[J].农业环境科学学报,2017,36(3):466-473.
[3] 张建莹,岳振峰,蔡伊娜.超高效液相色谱-串联质谱法测定复杂基质食品中二硝基苯胺类除草剂残留量[J].分析测试学报,2017,36(4):550-554.
[4]Lopes R M,Corvo M L,Eleuterio C V,et al.Formulation of oryzalin (ORZ) liposomes:in vitro studies and in vivo fate[J].European Journal of Pharmaceutics and Biopharmaceutics,2012,82(2):281-290.
[5] Dvorakova K,Dorr R T,Gallegos A,et al.Pharmacokinetic studies of the herbicide and antitumor compound oryzalin in mice[J].Journal of chromatography B,Biomedical Sciences and Applications,1997,696(2):275-281.
[6]Li D,Zhou D,Wang P,et al.Temperature affects cadmium-induced phytotoxicity involved in subcellular cadmium distribution and oxidative stress in wheat roots[J].Ecotoxicology and Environmental Safety,2011,74(7):2029-2035.
[7] Zhang Q M,Zhang B H,Wang C X.Ecotoxicological effectson the earthworm Eisenia fetida following exposure to soil contaminated with imidacloprid[J].Environmental Science and Pollution Research,2014,21(21) :12345-12353.
[8] Wang Y H,Chen C,Qian Y Z,et al.Ternary toxicological interactions of insecticides,herbicides,and a heavy metal on the earthworm Eisenia fetida[J].Journal of Hazardous Materials,2015,284:233-240.
[9] Zhang Q M,Zhang G L,Yin P J,et al.Toxicological effects of soil contaminated with spirotetramat to the earthworm Eisenia fetida[J].Chemosphere,2015,139:138-145.
[10]周垂帆.重金属和草甘膦复合污染生态毒理研究[D].南京:南京林业大学,2013.
[11] 赵丽倩,仇爱锋,纪伟,等.亚致死剂量高效氯氰菊酯对蚯蚓体内生化指标的影响[J].生态科学,2018,37(4):65-71.
[12]平令文,李现旭,张翠,等.DEP 对蚯蚓抗氧化酶系的影响及 DNA 损伤[J].环境科学,2018,39(10):4825-4833.
[13]曹四平,刘长海.两种生物农药对蚯蚓的急性毒性及氧化胁迫效应[J].甘肃科学学报,2017,29(5):67-72.
[14]贾茹,王梦远,黄擎.土壤辛硫磷污染对蚯蚓的生态毒理效应研究[J].环境污染与防治,2017,39(9):943-947.
[15] 冯少君,程华民,陶菁,等.全氟辛酸暴露对蚯蚓DNA 损伤和抗氧化酶基因表达水平的影响[J].热带生物学报,2016,7(2):253-258.
[16] 王晓蓉,罗义,施华宏,等.分子生物标志物在污染环境早期诊断和生态风险评价中的应用[J].环境化学,2006,25(3):320-325.
[17] 肖广侠,徐文远,贾磊,等.悬浮物对褐牙鲆抗氧化酶活性及相关基因表达的影响[J].生态毒理学报,2016,11(6):266-276.