3种不同结构芸苔素内酯在棉花上的应用研究
|
摘要
为了确定3种不同结构的芸苔素内酯(24-表、28-高和28-表高)在棉花上的最佳调节效果和使用剂量,本研究分别在室内和田间进行了试验。室内试验采用浸种的方法,田间药效试验选择在棉花苗期、蕾铃期和吐絮期分别进行茎叶喷雾处理来进行。结果表明:当浸种剂量为0.01 mg/kg时,3种不同结构的芸苔素内酯对棉花种子的发芽势和根系活力都有显着的增加。与未处理对照相比,当施药剂量为0.02 mg/kg时,芸苔素内酯可以明显提高棉铃重、棉纤维长度和棉花整齐度,并且也能一定程度的增加棉花产量。在室内试验和田间试验中,28-高在3种不同结构的芸苔素内酯中都表现出最佳的调节效果。因此,为了调节植株生长以及提高棉花的品质和产量,推荐使用具有28-高结构的芸苔素内酯,其中浸种浓度为0.01～0.02 mg/kg,田间喷雾剂量为0.02～0.04 mg/kg。试验期间,棉花长势良好没有药害发生。
To determine the optimal regulation effect and the most efficient dosage of three different structural brassinolides(24-Epi, 28-Homo and 28-Epihomo) on cotton, laboratory experiment and field experiment were conducted. Indoor experiment was conducted with seed soaking, and field trials were carried out with stem and leaf spray at the seedling stage, boll stage and boll opening stage. The results showed that when the seed soaking dose was 0.01 mg/kg, the germination force and root vitality of cotton seeds were increased significantly with the three different structural brassinolide treatments. Compared with the control, when the spray dosage was 0.02 mg/kg, brassinolide improved the weight of bolls, fiber length and cotton uniformity, and also increased the yield to a certain extent. 28-Homo showed the best effect among the three brassinolides both in laboratory and field experiment. Therefore, to regulate the growth of cotton plants and increase the quality and yield of cotton, 28-Homo is recommended, and the seed soaking dosage is 0.01-0.02 mg/kg and the spray dosage is 0.02-0.04 mg/kg. There is no injury on cotton during the period of experiment.
To determine the optimal regulation effect and the most efficient dosage of three different structural brassinolides(24-Epi, 28-Homo and 28-Epihomo) on cotton, laboratory experiment and field experiment were conducted. Indoor experiment was conducted with seed soaking, and field trials were carried out with stem and leaf spray at the seedling stage, boll stage and boll opening stage. The results showed that when the seed soaking dose was 0.01 mg/kg, the germination force and root vitality of cotton seeds were increased significantly with the three different structural brassinolide treatments. Compared with the control, when the spray dosage was 0.02 mg/kg, brassinolide improved the weight of bolls, fiber length and cotton uniformity, and also increased the yield to a certain extent. 28-Homo showed the best effect among the three brassinolides both in laboratory and field experiment. Therefore, to regulate the growth of cotton plants and increase the quality and yield of cotton, 28-Homo is recommended, and the seed soaking dosage is 0.01-0.02 mg/kg and the spray dosage is 0.02-0.04 mg/kg. There is no injury on cotton during the period of experiment.
引文
[1]王友华,束红梅,陈兵林,等.不同棉花品种纤维比强度形成的时空差异及其与温度的关系[J].中国农业科学,2008,41(11):3865-3871.
[2]韩云,闫凯莉,吴建辉,等.0.0075%芸苔素内酯水剂对小白菜生长和品质的影响[J].中国农学通报,2015,31(13):81-84.
[3]Mulqueen P. Recent advances in agrochemical formulation[J]. Adv Colloid Interface Sci, 2003,106(1):83-107.
[4]乔康,姬小雪,邱士芬,等.芸苔素内酯0.01%水剂调节黄瓜生长田间药效试验[J].生物灾害科学,2012(4):410-412.
[5]胡文海,黄黎锋,毛伟华,等.油菜素内酯对黄瓜苗期叶片光合机构调节作用的研究[J].园艺学报,2006,33(4):762-766.
[6]张兴华,李捷.氯吡脲和芸苔素内酯对棉花田草甘膦药害的解毒效果[J].农药,2008,47(11):834-835.
[7]王江柱,裴敬祖,崔彦宏.丙酰芸苔素内酯对小麦增产效果的影响[J].中国农学通报,2007,23(4):245-247.
[8]周小毛,黄雄英,柏连阳,等.芸苔素内酯保护玉米免受胺苯磺伤害的作用及其机理[J].植物保护学报,2005,32(2):189-194.
[9]安亚杰.你所不知道的芸苔素内酯[J].营销界(农资与市场), 2017(9):36-39.
[10]黄斌荣.28-高芸苔素内酯的合成工艺研究[D].南昌:南昌大学,2015.
[11]Heydecker W, Higgins, Amp J, et al. Accelerated Germination by Osmotic Seed Treatment[J]. Nature, 1973, 246(5427):42-44.
[12]赵海泉,洪法水.钼浸种对紫花苜蓿种子活力和产量的影响[J].中国草地学报,1998(1):74-79.
[13]刘慧霞,王彦荣.水引发对紫花苜蓿种子萌发及其生理活动的影响[J].草业学报, 2008,17(4):78-84.
[14]Chen K, Arora R. Priming memory invokes seed stress-tolerance[J].Environmental&Experimental Botany, 2013, 94(6):33-45.
[15]刘伟,王金信,杨广玲,等.芸苔素内酯对花生幼苗生长的影响[J].现代农药,2005,4(1):42-43.
[16]徐静,王晓双,李佳颖,等.6.003%氨基·芸苔素内酯水剂对芒果树生长、品质和产量的影响[J].农药,2018(3):232-234.
[17]Hermann M L, Russell J R, Barnhart S K. Evaluation of hay-type and grazing-tolerant alfalfa cultivars in season-long or complementary rotational stocking systems for beef cows[J].Journal of Animal Science, 2002, 80(3):768.
[18]李洁,徐军桂,林程,等.引发对低温胁迫下不同类型玉米种子萌发及幼苗生理特性的影响[J].植物生理学报,2016(2):157-166.
[19]叶梅荣. NaCl胁迫下水杨酸浸种对水稻幼苗生长的影响[J].安徽科技学院学报, 2002,16(4):44-46.
[20]Bai B Z, Jin J Z, Bai S. Improvement of TTC method determining root activity in corn[J]. Maizeence, 1994.
[21]单世华,孙学振,周治国,等.温度对棉纤维品质性状的影响[J].华北农学报,2000,15(4):120-125.
[22]罗维.浅析植物生长调节剂芸苔素内酯[J].广州化工, 2013,41(15):33-35.
[23]Koka C V, Cerny R E, Gardner R G, et al. A Putative Role for the Tomato Genes DUMPY and CURL-3 in Brassinosteroid Biosynthesis and Response[J]. Plant Physiology, 2000, 122(1):85-98.
[24]中华人民共和国农业部.到2020年农药使用量零增长行动方案[J].青海农技推广, 2015(2):6-8.
[25]张楠.植物生长调节剂的种类、作用及注意事项[J].河南农业,2018(7):36,50.
[26]黄再萍.芸苔素内酯复配制剂的开发与应用[D].南昌:江西农业大学,2013.
[27]Poulton C, Gibbon P, Hanyanimlambo B, et al. Competition and coordination in liberalized African cotton market systems[J]. World Development, 2004, 32(3):519-536.
[28]雷亚平,魏晓文,刘志红,等.中国棉花产业发展现状及展望[J].农业展望,2014,10(9):43-47.
[29]何剑星.马克隆值是棉花质量的重要参数[J].中国棉花加工,2005(3):25-26.
[30]卞康亚,胡晓荣,吉净.0.01%芸苔素内酯可溶液剂调节棉花生长药效试验[J].上海蔬菜,2014(1):71.
[31]严晓红,包秋娟,张富春.干旱胁迫下油菜素内酯浸种对棉花种子萌发期NAC转录因子表达的影响[J].新疆农业科学,2017,54(8):1414-1421.
[2]韩云,闫凯莉,吴建辉,等.0.0075%芸苔素内酯水剂对小白菜生长和品质的影响[J].中国农学通报,2015,31(13):81-84.
[3]Mulqueen P. Recent advances in agrochemical formulation[J]. Adv Colloid Interface Sci, 2003,106(1):83-107.
[4]乔康,姬小雪,邱士芬,等.芸苔素内酯0.01%水剂调节黄瓜生长田间药效试验[J].生物灾害科学,2012(4):410-412.
[5]胡文海,黄黎锋,毛伟华,等.油菜素内酯对黄瓜苗期叶片光合机构调节作用的研究[J].园艺学报,2006,33(4):762-766.
[6]张兴华,李捷.氯吡脲和芸苔素内酯对棉花田草甘膦药害的解毒效果[J].农药,2008,47(11):834-835.
[7]王江柱,裴敬祖,崔彦宏.丙酰芸苔素内酯对小麦增产效果的影响[J].中国农学通报,2007,23(4):245-247.
[8]周小毛,黄雄英,柏连阳,等.芸苔素内酯保护玉米免受胺苯磺伤害的作用及其机理[J].植物保护学报,2005,32(2):189-194.
[9]安亚杰.你所不知道的芸苔素内酯[J].营销界(农资与市场), 2017(9):36-39.
[10]黄斌荣.28-高芸苔素内酯的合成工艺研究[D].南昌:南昌大学,2015.
[11]Heydecker W, Higgins, Amp J, et al. Accelerated Germination by Osmotic Seed Treatment[J]. Nature, 1973, 246(5427):42-44.
[12]赵海泉,洪法水.钼浸种对紫花苜蓿种子活力和产量的影响[J].中国草地学报,1998(1):74-79.
[13]刘慧霞,王彦荣.水引发对紫花苜蓿种子萌发及其生理活动的影响[J].草业学报, 2008,17(4):78-84.
[14]Chen K, Arora R. Priming memory invokes seed stress-tolerance[J].Environmental&Experimental Botany, 2013, 94(6):33-45.
[15]刘伟,王金信,杨广玲,等.芸苔素内酯对花生幼苗生长的影响[J].现代农药,2005,4(1):42-43.
[16]徐静,王晓双,李佳颖,等.6.003%氨基·芸苔素内酯水剂对芒果树生长、品质和产量的影响[J].农药,2018(3):232-234.
[17]Hermann M L, Russell J R, Barnhart S K. Evaluation of hay-type and grazing-tolerant alfalfa cultivars in season-long or complementary rotational stocking systems for beef cows[J].Journal of Animal Science, 2002, 80(3):768.
[18]李洁,徐军桂,林程,等.引发对低温胁迫下不同类型玉米种子萌发及幼苗生理特性的影响[J].植物生理学报,2016(2):157-166.
[19]叶梅荣. NaCl胁迫下水杨酸浸种对水稻幼苗生长的影响[J].安徽科技学院学报, 2002,16(4):44-46.
[20]Bai B Z, Jin J Z, Bai S. Improvement of TTC method determining root activity in corn[J]. Maizeence, 1994.
[21]单世华,孙学振,周治国,等.温度对棉纤维品质性状的影响[J].华北农学报,2000,15(4):120-125.
[22]罗维.浅析植物生长调节剂芸苔素内酯[J].广州化工, 2013,41(15):33-35.
[23]Koka C V, Cerny R E, Gardner R G, et al. A Putative Role for the Tomato Genes DUMPY and CURL-3 in Brassinosteroid Biosynthesis and Response[J]. Plant Physiology, 2000, 122(1):85-98.
[24]中华人民共和国农业部.到2020年农药使用量零增长行动方案[J].青海农技推广, 2015(2):6-8.
[25]张楠.植物生长调节剂的种类、作用及注意事项[J].河南农业,2018(7):36,50.
[26]黄再萍.芸苔素内酯复配制剂的开发与应用[D].南昌:江西农业大学,2013.
[27]Poulton C, Gibbon P, Hanyanimlambo B, et al. Competition and coordination in liberalized African cotton market systems[J]. World Development, 2004, 32(3):519-536.
[28]雷亚平,魏晓文,刘志红,等.中国棉花产业发展现状及展望[J].农业展望,2014,10(9):43-47.
[29]何剑星.马克隆值是棉花质量的重要参数[J].中国棉花加工,2005(3):25-26.
[30]卞康亚,胡晓荣,吉净.0.01%芸苔素内酯可溶液剂调节棉花生长药效试验[J].上海蔬菜,2014(1):71.
[31]严晓红,包秋娟,张富春.干旱胁迫下油菜素内酯浸种对棉花种子萌发期NAC转录因子表达的影响[J].新疆农业科学,2017,54(8):1414-1421.