苜蓿盲蝽属三种害虫对环氧虫啶的敏感性
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摘要
环氧虫啶是我国自主研发的一种新型的新烟碱杀虫剂,主要用于防治刺吸式口器害虫。本文采用点滴法测定了环氧虫啶对中黑盲蝽、苜蓿盲蝽、三点盲蝽的毒力,结果表明,三种盲蝽对环氧虫啶均具有较高的敏感性,其中中黑盲蝽的敏感性最高,LD_(50)值为2.03ng/头,其次为苜蓿盲蝽和三点盲蝽,LD_(50)分别为3.12和3.34ng/头。多功能氧化酶抑制剂PBO能明显增加环氧虫啶对三种盲蝽的毒力,谷胱甘肽S-转移酶抑制剂DEM只在中黑盲蝽上对环氧虫啶表现出增效作用,而羧酸酯酶抑制剂TPP未显示增效作用。这说明,环氧虫啶具有防治苜蓿盲蝽属三种害虫的应用潜力,细胞色素P450-单加氧酶可能在环氧虫啶代谢中起重要作用。
Cycloxaprid is a novel self-developed neonicotinoid insecticide for the control of sucking insect pests.Three Adelphocoris species(Hemiptera:Miridae),A.suturalis,A.lineolatus,and A.fasciaticollis,are important insect pests in cotton,alfalfa and other crops in China.Bioassays of cycloxaprid showed that A.suturalis(LD_(50):2.03 ng/adult)was more susceptible to cycloxaprid than A.lineolatus and A.fasciaticollis with LD_(50) values of 3.12 and 3.34 ng/adult,respectively.The toxicity of cycloxaprid to three Adelphocoris species could be synergized by piperonyl butoxide(PBO),but generally not by TPP.Inhibitor of glutathione S-transferase(GST),the DEM,showed synergism only to cycloxaprid against A.suturalis.The results suggest that cycloxaprid is a candidate for controlling Adelphocoris species.Cytochrome P450-dependent monooxygenase might play an important role in the metabolism of cycloxaprid in these three mirid bugs.
Cycloxaprid is a novel self-developed neonicotinoid insecticide for the control of sucking insect pests.Three Adelphocoris species(Hemiptera:Miridae),A.suturalis,A.lineolatus,and A.fasciaticollis,are important insect pests in cotton,alfalfa and other crops in China.Bioassays of cycloxaprid showed that A.suturalis(LD_(50):2.03 ng/adult)was more susceptible to cycloxaprid than A.lineolatus and A.fasciaticollis with LD_(50) values of 3.12 and 3.34 ng/adult,respectively.The toxicity of cycloxaprid to three Adelphocoris species could be synergized by piperonyl butoxide(PBO),but generally not by TPP.Inhibitor of glutathione S-transferase(GST),the DEM,showed synergism only to cycloxaprid against A.suturalis.The results suggest that cycloxaprid is a candidate for controlling Adelphocoris species.Cytochrome P450-dependent monooxygenase might play an important role in the metabolism of cycloxaprid in these three mirid bugs.
引文
[1]姜玉英,陆宴辉,曾娟.盲蝽分区监测与治理[M].北京:中国农业出版社,2015.
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[12]陆宴辉,吴孔明,蔡晓明,等.利用四季豆饲养盲蝽的方法[J].植物保护学报,2008,35(3):215-219.
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[15]Nauen R,Denholm I.Resistance of insect pests to neonicotinoid insecticides:current status and future prospects[J].Archives of Insect Biochemistry and Physiology,2005,58(4):200-215.
[16]Roditakis E,Grispou M,Morou E,et al.Current status of insecticide resistance in Q biotype Bemisia tabaci populations from crete[J].Pest Management Science,2009,65(3):313-322.
[17]姚明德.B型烟粉虱抗吡虫啉品系的交互抗性谱及抗性生化机理[D].南京:南京农业大学,2007.
[18]封云涛.B型烟粉虱入侵种群对噻虫嗪抗性机理的研究[D].北京:中国农业科学院,2009.
[19]郭天凤,史雪岩,高希武,等.棉蚜吡虫啉、啶虫脒不同品系解毒酶活性测定和增效作用的研究[J].环境昆虫学报,2014,36(3):388-394.
[20]Cui Li,Qi Haoliang,Yang Daibin,et al.Cycloxaprid:a novel cis-nitromethylene neonicotinoid insecticide to control imidacloprid-resistant cotton aphid(Aphis gossypii)[J].Pesticide Biochemistry and Physiology,2016,132:96-101.
[21]Valles S M,Yu S J.Detection and biochemical characterization of insecticide resistance in the German cockroach(Dictyoptera:Blattellidae)[J].Journal of Economic Entomology,1996,89(1):21-26.
[22]刘喃喃,朱芳,徐强,等.昆虫抗药性机理:行为和生理改变及解毒代谢增强[J].昆虫学报,2006,49(4):671-679.
[23]Gao Congfen,Ma Shaozhi,Shan Caihui,et al.Thiamethoxam resistance selected in the western flower thrips Frankliniella occidentalis(Thysanoptera:Thripidae):Cross-resistance patterns,possible biochemical mechanisms and fitness costs analysis[J].Pesticide Biochemistry and Physiology,2014,114(5):90-96.
[24]Zhu Yucheng,Blanco C A,Portilla M,et al.Evidence of multiple/cross resistance to Bt and organophosphate insecticides in Puerto Rico population of the fall armyworm,Spodoptera frugiperda[J].Pesticide Biochemistry and Physiology,2015,122:15-21.
[2]Lu Yanhui,Qiu Feng,Feng Hongqiang,et al.Species composition and seasonal abundance of pestiferous plant bugs(Hemiptera:Miridae)on Bt cotton in China[J].Crop Protection,2008,27:465-472.
[3]陆宴辉,吴孔明.棉花盲蝽象及其防治[M].北京:金盾出版社,2008.
[4]Lu Yanhui,Wu Kongming,Wyckhuys K A G,et al.Comparative flight performance of three important pest Adelphocoris species of Bt cotton in China[J].Bulletin of Entomological Research,2009,99(6):543-550.
[5]Shao Xusheng,Fu Hua,Xu Xiaoyong,et al.Divalent and oxabridged neonicotinoids constructed by dialdehydes and nitromethylene analogues of imidacloprid:design,synthesis,crystal structure,and insecticidal activities[J].Journal of Agricultural and Food Chemistry,2010,58(5):2696-2702.
[6]Shao Xusheng,Lee P W,Liu Zewen,et al.Cis-configuration:A new tactic/rationale for neonicotinoid molecular design[J].Journal of Agricultural and Food Chemistry,2011,59(7):2943-2949.
[7]Cui Li,Sun Lina,Yang Daibin,et al.Effects of cycloxaprid,a novel cis-nitromethylene neonicotinoid insecticide,on the feeding behavior of Sitobion avenae[J].Pest Management Science,2012,68(11):1484-1491.
[8]刘宝生,张志春,谢霖,等.新药剂环氧虫啶对稻飞虱的杀虫活性和田间效果[J].西南农业学报,2013,26(1):155-158.
[9]Wang Ran,Zheng Huixin,Qu Cheng,et al.Lethal and sublethal effects of a novel cis-nitromethylene neonicotinoid insecticide,cycloxaprid,on Bemisia tabaci[J].Crop Protection,2016,83:15-19.
[10]Shao Xusheng,Liu Zewen,Xu Xiaoyong,et al.Overall status of neonicotinoid insecticides in China:Production application and innovation[J].Journal of Pesticide Science,2013,38(1):1-9.
[11]Pan Hongsheng,Liu Yongqiang,Liu Bing,et al.Lethal and sublethal effects of cycloxaprid,a novel cis-nitromethylene neonicotinoid insecticide,on the mirid bug Apolygus lucorum[J].Journal of Pest Science,2014,87(4):731-738.
[12]陆宴辉,吴孔明,蔡晓明,等.利用四季豆饲养盲蝽的方法[J].植物保护学报,2008,35(3):215-219.
[13]Desneux N,Denoyelle R,Kaiser L.A multi-step bioassay to assess the effect of the deltamethrin on the parasitic wasp Aphidius ervi[J].Chemosphere,2006,65(10):1697-1706.
[14]Scott J G,Georghiou G P.Mechanisms responsible for high levels of permethrin resistance in housefly[J].Pesticide Science,1986,17(3):195-206.
[15]Nauen R,Denholm I.Resistance of insect pests to neonicotinoid insecticides:current status and future prospects[J].Archives of Insect Biochemistry and Physiology,2005,58(4):200-215.
[16]Roditakis E,Grispou M,Morou E,et al.Current status of insecticide resistance in Q biotype Bemisia tabaci populations from crete[J].Pest Management Science,2009,65(3):313-322.
[17]姚明德.B型烟粉虱抗吡虫啉品系的交互抗性谱及抗性生化机理[D].南京:南京农业大学,2007.
[18]封云涛.B型烟粉虱入侵种群对噻虫嗪抗性机理的研究[D].北京:中国农业科学院,2009.
[19]郭天凤,史雪岩,高希武,等.棉蚜吡虫啉、啶虫脒不同品系解毒酶活性测定和增效作用的研究[J].环境昆虫学报,2014,36(3):388-394.
[20]Cui Li,Qi Haoliang,Yang Daibin,et al.Cycloxaprid:a novel cis-nitromethylene neonicotinoid insecticide to control imidacloprid-resistant cotton aphid(Aphis gossypii)[J].Pesticide Biochemistry and Physiology,2016,132:96-101.
[21]Valles S M,Yu S J.Detection and biochemical characterization of insecticide resistance in the German cockroach(Dictyoptera:Blattellidae)[J].Journal of Economic Entomology,1996,89(1):21-26.
[22]刘喃喃,朱芳,徐强,等.昆虫抗药性机理:行为和生理改变及解毒代谢增强[J].昆虫学报,2006,49(4):671-679.
[23]Gao Congfen,Ma Shaozhi,Shan Caihui,et al.Thiamethoxam resistance selected in the western flower thrips Frankliniella occidentalis(Thysanoptera:Thripidae):Cross-resistance patterns,possible biochemical mechanisms and fitness costs analysis[J].Pesticide Biochemistry and Physiology,2014,114(5):90-96.
[24]Zhu Yucheng,Blanco C A,Portilla M,et al.Evidence of multiple/cross resistance to Bt and organophosphate insecticides in Puerto Rico population of the fall armyworm,Spodoptera frugiperda[J].Pesticide Biochemistry and Physiology,2015,122:15-21.