内源激活剂驱油藏内微生物菌群结构变化分析
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摘要
油藏内源功能微生物的激活能有效提高采油效率。其中激活后功能微生物的群落变化分析及相关代谢基因丰度的变化对内源激活剂的筛选及激活机理研究具有重要的指导意义。利用高通量测序技术准确分析了内源激活剂注入井(WJW199. 1)和对照井(WJW202)中内源微生物(包括古菌和细菌)基于门、纲、属水平的种群多样性和群落结构。结果表明采出水中Thaumarchaeota门的古菌占主导,而丰度最高的细菌属于Proteobacteria门和Firmicutes门。不管对于古菌还是细菌,注入井中的微生物多样性都远高于对照井,说明激活剂的添加有效激活了其中的内源微生物,提高了群落结构多样性。尤其是Pseudomonas、Arcobacter等具有产表活剂能力的功能微生物。同时,硫酸盐还原菌脱硫弧菌属等有害菌被抑制,丰度降低。表明从微生物种群变化的程度来看内源激活剂的注入降低了有害菌的丰度,激活了功能微生物,增强了整体微生物的驱油能力。
The activation of endogenous functional microorganisms in the reservoir can effectively improve oil recovery efficiency. The analysis changes of functional microbial community after activation and related metabolic gene abundance have important guiding significance for the screening and activation mechanism of endogenous activators. High-throughput sequencing technology was used to accurately analyze the microbial diversity of endogenous microorganisms( including archaea and bacteria) in endogenous activator injection wells( WJW199. 1) and control wells( WJW202) in the level of phylum,class and genus. The results showed that the in the produced water the dominated archaea belong to Thaumarchaeota phylum,while the most abundant bacteria belonged to the Proteobacteria and the Firmicutes phylum. Regardless of archaea or bacteria,the microbial diversity in the activator-injection well was much higher than that of the control well,indicating that the injection of the activator effectively activated the endogenous microorganisms and improves the diversity of the microbial community,especially for Pseudomonas,Arcobacter genus which could produce surfactants. Simultaneously,harmful bacteria such as sulfate-reducing bacteria Desulfovibrio were inhibited and the abundance is decreased. In conclusion,the injection of endogenous activator reduced the abundance of harmful microorganisms,increased the of functional microbial population,leading to the enhancement of oil recovery.
The activation of endogenous functional microorganisms in the reservoir can effectively improve oil recovery efficiency. The analysis changes of functional microbial community after activation and related metabolic gene abundance have important guiding significance for the screening and activation mechanism of endogenous activators. High-throughput sequencing technology was used to accurately analyze the microbial diversity of endogenous microorganisms( including archaea and bacteria) in endogenous activator injection wells( WJW199. 1) and control wells( WJW202) in the level of phylum,class and genus. The results showed that the in the produced water the dominated archaea belong to Thaumarchaeota phylum,while the most abundant bacteria belonged to the Proteobacteria and the Firmicutes phylum. Regardless of archaea or bacteria,the microbial diversity in the activator-injection well was much higher than that of the control well,indicating that the injection of the activator effectively activated the endogenous microorganisms and improves the diversity of the microbial community,especially for Pseudomonas,Arcobacter genus which could produce surfactants. Simultaneously,harmful bacteria such as sulfate-reducing bacteria Desulfovibrio were inhibited and the abundance is decreased. In conclusion,the injection of endogenous activator reduced the abundance of harmful microorganisms,increased the of functional microbial population,leading to the enhancement of oil recovery.
引文
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19李彩风,李阳,曹嫣镔,等.油藏环境产脂肽类表面活性剂微生物的分布[J].石油学报,2015,36(9):1122-1126Li Caifeng,Li Yang,Cao Yanbin,et al.Distribution of the lipopeptide biosurfactant-producing microbes in oil reservoir environment[J].Acta Petrolei Sinica,2015,36(9):1122-1126
2 Li X X,Liu J F,Zhou L,et al.Diversity and composition of sulfatereducing microbial communities based on genomic DNA and RNAtranscription in production water of high temperature and corrosive oil reservoir[J].Frontiers in Microbiology,2017,8(8):1011
3高配科,马挺,刘如林.油藏微生物的代谢特征和生态结构调控[J].微生物学报,2011,51(6):711-717Gao Peike,Ma Ting,Liu Rulin.Microbial metabolic characteristics and ecological controlling in petroleum reservoir-A review[J].Acta Microbiologica Sinica,2011,51(6):711-717
4王继波.内源菌采油实施效果与应用潜力分析[J].中国石油石化,2017(9):97-98Wang Jibo.Analysis of implementation effect and application potential of endogenous bacterial production[J].China Petrochem,2017(9):97-98
5 Simpson D R,Nisha R N,Michael J M,et al.Biosurfactant-producing bacillus are present in produced brines from Oklahoma oil reservoirs with a wide range ofsalinities[J].Applied Microbiology and Biotechnology,2011,91(4):1083-1093
6宋永亭.油藏内源产表面活性剂微生物的选择性激活[D].青岛:中国海洋大学,2012Song Yongting.Selective stimulation of indigenous biosurfactant-producing microbes in the oil reservoir[D].Qingdao:Ocean University of China,2012
7 Pace N R.A molecular view of microbial diversity and the biosphere[J].Science,1997,276(5313):734-740
8 Blaxter M,Mann J,Chapman T,et al.Defining operational taxonomic units using DNA barcode data[J].Philosophical Transactions of the Royal Society B-Biological Sciences,2005,360(1462);1935-1943
9 Edgar R,Qimron U.The Escherichia coli crispr system protects from lambda lysogenization,lysogens,and prophage induction[J].Journal of Bacteriology,2010,192(23):6291-6294
10 Kemp P F,Aller J Y.Estimating prokaryotic diversity:When are16S r DNA libraries large enough[J].Limnology and OceanographyMethods,2004,2(1):114-125
11 White B R,Snyder A Z,Cohen A L,et al.Resting-state functional connectivity in the human brain revealed with diffuse optical tomography[J].Neuroimage,2009,47(1):148-156
12 Langille M G I,Zaneveld J,Caporaso J G,et al.Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences[J].Nature Biotechnology,2013,31(9):814
13王大威,张健,何春百,等.渤海N油田油藏内源微生物群落结构分析[J].生物技术通报,2016,32(9):83-92Wang Dawei,Zhang Jian,He Chunbai,et al.Research on population structures and distribution characteristics of indigenous microorganisms in Bohai Oil Field N[J].Biotechnology Bulletin,2016,32(9):83-92
14宋智勇,郝滨,赵凤敏,等.胜利油田水驱油藏内源微生物群落结构及分布规律[J].西安石油大学学报(自然科学版),2013,28(4):44-50Song Zhiyong,Hao Bin,Zhao Fengmin,et al.Communiy structure and distribution of endogenous microorganism in warter flooding reserviors of Shengli Oilfield[J].Journal of Xi'an Shiyou University(Natural Science Edition),2013,28(4):44-50
15 Thomsen T R,Kong Y,Nielsen P H.Ecophysiology of abundant denitrifying bacteria in activated sludge[J].Fems Microbiology Ecology,2007,60(3):370-382
16高配科,马挺,赵玲侠,等.胜利油田沾3区块内源微生物激活剂的筛选、优化及效果评价[J].化工学报,2011,62(7):2005-2012Gao Peike,Ma Ting,Zhao Lingxia,et al.Screening,optimization and evaluation of indigenous microorganism activation system in Zhan3,Shengli Oilfield[J].Journal of Chemical Industry and Engineering(China),2011,62(7):2005-2012
17崔庆锋,齐义彬,伊丽娜,等.氯化钙水型油藏内源微生物激活剂研究[J].科学技术与工程,2015,15(4):78-83Cui Qingfeng,Qi Yibin,Yi Lina,et al.Study on indigenous microorganism activator in a oil reservoir of calcium chloride water[J].Science Technology and Engineering,2015,15(4):78-83
18许颖,马德胜,宋文枫,等.采用16SrDNA高通量测序技术分析油藏微生物多样性[J].应用与环境生物学报,2016,22(3):409-414Xu Ying,Ma Desheng,Song Wenfeng,et al.16S r DNA-assisted high-throughput sequencing analysis of microbial diversity in oil reservoirs[J].Chinese Journal of Applied and Environmental Biology,2016,22(3):409-414
19李彩风,李阳,曹嫣镔,等.油藏环境产脂肽类表面活性剂微生物的分布[J].石油学报,2015,36(9):1122-1126Li Caifeng,Li Yang,Cao Yanbin,et al.Distribution of the lipopeptide biosurfactant-producing microbes in oil reservoir environment[J].Acta Petrolei Sinica,2015,36(9):1122-1126