高锰酸钾-甲醛化学发光体系测定药物甲磺酸酚妥拉明的含量
|
摘要
在酸性条件下,高锰酸钾氧化甲磺酸酚妥拉明产生化学发光信号,加入甲醛可以提高化学发光强度,因此建立一种新型的利用高锰酸钾-甲醛化学发光体系测定药物甲磺酸酚妥拉明含量的方法.在最佳实验条件下,药物甲磺酸酚妥拉明的浓度在0.05-4.00μg·mL-1的范围内线性关系良好,检出限为0.01μg·mL-1,回归方程为:I=15.587c+2.362 2,r=0.998 8,RSD为1.6%,平均回收率为100.5%.本方法用于药物甲磺酸酚妥拉明含量的测定,结果满意.
Under acidic conditions,phtholaminie mesilate could be oxidized by potassium permanganate to produce chemiluminescence(CL)intensity,and the CL intensity could be improved by adding formaldehyde.In this experiment,a new method was established for the determination of phenololamine mesilate.In the best conditions,the liner range of the method which determined the concentration of the phtholaminie mesilate was 0.05-4.00μg·mL-1 with a detection 0.01μg·mL-1.The linear equation was I=15.587 c+2.3622,r=0.9988,RSD=1.6%.The average recovery was 100.5%.This method was applied for the determination of the content of phentolamine mesilate in drugs,and the result was satisfactory.
Under acidic conditions,phtholaminie mesilate could be oxidized by potassium permanganate to produce chemiluminescence(CL)intensity,and the CL intensity could be improved by adding formaldehyde.In this experiment,a new method was established for the determination of phenololamine mesilate.In the best conditions,the liner range of the method which determined the concentration of the phtholaminie mesilate was 0.05-4.00μg·mL-1 with a detection 0.01μg·mL-1.The linear equation was I=15.587 c+2.3622,r=0.9988,RSD=1.6%.The average recovery was 100.5%.This method was applied for the determination of the content of phentolamine mesilate in drugs,and the result was satisfactory.
引文
[1]李青翠,李芙蓉,邓自新,等.甲磺酸酚妥拉明片稳定性考察[J].山西医科大学学报,1999,30(增刊):71-72.
[2]孙玲,徐惠.高效液相色谱法测定甲磺酸酚妥拉明注射剂的含量[J].山东医药工业,1999,18(05):14-15.
[3]李文英,常俊兰.HPLC测定甲磺酸酚妥拉明片有关物质[J].中国药学杂志,2000,35(01):44.
[4]翁水旺,沙吉达.HPLC法测定甲磺酸酚妥拉明分散片的含量[J].中国现代应用药学杂志,2000,17(06):474-476.
[5]单利,高春生,崔光华.反相高效液相色谱法测定甲磺酸酚妥拉明速崩片的含量[J].药物分析杂志,2000,20(01):41-43.
[6]蒋丽萍,屠婕红,徐宏祥,等.UPLC-MS/MS法测定抗疲劳类保健食品中非法添加的9种壮阳类化学药物[J].中草药,2015,46(15):2239-2245.
[7]王丽珍.紫外分光光度法测定甲磺酸酚妥拉明注射液的含量[J].江苏药学与临床研究,1998,06(01):23-24.
[8]翁水旺,刘解民.紫外分光光度法测定甲磺酸酚妥拉明分散片的含量[J].解放军药学学报,2000,16(02):107-109.
[9]邓自新,梁小军.紫外分光光度法测定甲磺酸酚妥拉明片的含量[J].山西医科大学学报,2000,31(1):42-43.
[10]马鲁豫,董伍爱.紫外分光光度法测定甲磺酸酚妥拉明片的含量[J].华西药学杂志,2001,02(06):95.
[11]张迎春,徐德泉,张迎雪.流动注射化学发光法测定注射液和人体血清中的酚妥拉明[J].海南师范大学学报(自然科学版),2007,20(03):264-267.
[12]牛玲梅,马莉,康维钧,等.荧光素及聚乙二醇增敏流动注射化学发光法测定甲磺酸酚妥拉明[J].河南医科大学学报,2008,29(02):242-244.
[2]孙玲,徐惠.高效液相色谱法测定甲磺酸酚妥拉明注射剂的含量[J].山东医药工业,1999,18(05):14-15.
[3]李文英,常俊兰.HPLC测定甲磺酸酚妥拉明片有关物质[J].中国药学杂志,2000,35(01):44.
[4]翁水旺,沙吉达.HPLC法测定甲磺酸酚妥拉明分散片的含量[J].中国现代应用药学杂志,2000,17(06):474-476.
[5]单利,高春生,崔光华.反相高效液相色谱法测定甲磺酸酚妥拉明速崩片的含量[J].药物分析杂志,2000,20(01):41-43.
[6]蒋丽萍,屠婕红,徐宏祥,等.UPLC-MS/MS法测定抗疲劳类保健食品中非法添加的9种壮阳类化学药物[J].中草药,2015,46(15):2239-2245.
[7]王丽珍.紫外分光光度法测定甲磺酸酚妥拉明注射液的含量[J].江苏药学与临床研究,1998,06(01):23-24.
[8]翁水旺,刘解民.紫外分光光度法测定甲磺酸酚妥拉明分散片的含量[J].解放军药学学报,2000,16(02):107-109.
[9]邓自新,梁小军.紫外分光光度法测定甲磺酸酚妥拉明片的含量[J].山西医科大学学报,2000,31(1):42-43.
[10]马鲁豫,董伍爱.紫外分光光度法测定甲磺酸酚妥拉明片的含量[J].华西药学杂志,2001,02(06):95.
[11]张迎春,徐德泉,张迎雪.流动注射化学发光法测定注射液和人体血清中的酚妥拉明[J].海南师范大学学报(自然科学版),2007,20(03):264-267.
[12]牛玲梅,马莉,康维钧,等.荧光素及聚乙二醇增敏流动注射化学发光法测定甲磺酸酚妥拉明[J].河南医科大学学报,2008,29(02):242-244.