高纯竹叶碳苷黄酮纳米材料的护肤功效
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摘要
在采用B16黑素瘤细胞体外评价高纯竹叶碳苷黄酮复合纳米材料(H-BLFnp)美白作用的基础上,基于临床医学理论进行人体实验,应用系列皮肤测试仪器,对受试者使用试样前后的皮肤含水量、水分散失量、光泽度、明亮度、黑色素含量、皮肤弹性等生理指标进行了测定,考察H-BLFnp在保湿、美白、延缓衰老方面的功效。结果显示,以β-熊果苷为阳性对照,H-BLFnp对B16细胞增殖、胞内酪氨酸酶活性和黑色素合成均有显著的抑制作用(p<0.05),当H-BLFnp质量浓度为100 mg/L时,其抑制率分别达到45.14%、72.55%和54.01%,即其可通过降低酪氨酸酶活性、抑制黑素细胞增殖和黑色素生成达到美白功效;人体实验结果显示:H-BLFnp能显著提高皮肤的水分含量、皮肤弹性、明亮度和光泽度,降低皮肤黑色素含量和经皮水分散失。
Based on the whitening effect of a novel composite nanomaterial taking high purity C-glycoside flavonoids of bamboo leaf as leading material(H-BLFnp) evaluated by B16 melanoma cells in vitro, its skin care function was further evaluated on the human body. The indexes of biological efficacy including skin moisture content, percutaneous moisture loss, skin gloss, brightness, melanin content and skin elasticity were tested before and after subjects using the samples to explore the biological efficacy of H-BLFnp on the moisturizing, whitening, anti-aging. The results showed that H-BLFnp significantly inhibited B16 cell proliferation, tyrosinase activity and melanin synthesis taking β-arbutin as a positive control(p<0.05), the corresponding inhibition rates achieved 45.14%, 72.55% and 54.01% respectively when the concentration of H-BLFnp was at 100 mg/L. H-BLFnp may exert whitening effect through inhibiting melanocyte proliferation, tyrosinase activity and melanin synthesis. The clinic test revealed that H-BLFnp significantly increased skin moisture content, skin elasticity, brightness and gloss, and reduced skin melanin content and percutaneous moisture loss.
Based on the whitening effect of a novel composite nanomaterial taking high purity C-glycoside flavonoids of bamboo leaf as leading material(H-BLFnp) evaluated by B16 melanoma cells in vitro, its skin care function was further evaluated on the human body. The indexes of biological efficacy including skin moisture content, percutaneous moisture loss, skin gloss, brightness, melanin content and skin elasticity were tested before and after subjects using the samples to explore the biological efficacy of H-BLFnp on the moisturizing, whitening, anti-aging. The results showed that H-BLFnp significantly inhibited B16 cell proliferation, tyrosinase activity and melanin synthesis taking β-arbutin as a positive control(p<0.05), the corresponding inhibition rates achieved 45.14%, 72.55% and 54.01% respectively when the concentration of H-BLFnp was at 100 mg/L. H-BLFnp may exert whitening effect through inhibiting melanocyte proliferation, tyrosinase activity and melanin synthesis. The clinic test revealed that H-BLFnp significantly increased skin moisture content, skin elasticity, brightness and gloss, and reduced skin melanin content and percutaneous moisture loss.
引文
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[2]Guan Xingli(官兴丽),Luo Liyong(罗理勇),Zeng Liang(曾亮).Advances at the research on the skin lightening effect and mechanism of natural products[J].Science and Technology of Food Industry(食品工业科技),2011,13(5):432-439.
[3]Deisinger P,Hill T,English J.Human exposure to naturally occurring hydroquinone[J].Journal of Toxicology and Environmental Health Part A,1996,47(1):31-46.
[4]Yang C,Chen Y,Lai J,et al.Determination of the termodegradation of deoxyarbutin in aqueous solution by high performance liquid chromatography[J].International Journal of Molecular Sciences,2010,11(10):3977-3987.
[5]Yang C,Chang N,Chen Y,et al.Comparative study on the photostability of arbutin and deoxy arbutin:Sensitivity to ultraviolet radiation and enhanced photostability by the water-soluble sunscreen,benzophenone-4[J].Bioscience,Biotechnology and Biochemistry,2013,77(5):1127-1130.
[6]Lin C,Yang C,Chang N,et al.Study on the stability of deoxy arbutin in an anhydrous emulsion system[J].International Journal of Molecular Sciences,2011,12(9):5946-5954.
[7]Degen G.Opinion of the scientific committee on consumer safety(SCCS)‐opinion on the safety of the use ofα-arbutin in cosmetic products.[J].Regulatory Toxicology&Pharmacology,2016,74(3):75-76.
[8]Degen G.Opinion of the scientific committee on consumer Safety(SCCS)‐opinion on the safety of the use ofβ-arbutin in cosmetic products[J].Regulatory Toxicology&Pharmacology,2015,73(3):388-389.
[9]Zhang Ying(张英).Natural functional extract of bamboo leavesbamboo leaf anthoxanthin[J].China Food Additives(中国食品添加剂),2002,(3):54-58.
[10]Lu B,Wu X,Tie X,et al.Toxicology and safety of anti-oxidant of bamboo leaves part 1:Acute and subchronic toxicity studies on anti-oxidant of bamboo leaves[J].Food and Chemical Toxicology,2005,43(5):783-792.
[11]Nirmala C,Bisht M,Bajwa H,et al.Bamboo:A rich source of natural antioxidants and its applications in the food and pharmaceutical industry[J].Trends in Food Science&Technology,2018,77:91-99.
[12]Hu C,Zhang Y,Kitts D.Evaluation of antioxidant and prooxidant activities of bamboo Phyllostachys nigra var.henonis leaf extract in vitro[J].Journal of Agricultural and Food Chemistry,2000,48(8):3170-3176.
[13]Poonam S,Lalit M,Santosh S,et al.Bamboo shoots:A novel source of nutrition and medicine[J].Critical Reviews in Food Science and Nutrition,2011,5(53):517-534.
[14]Zielińska D,Zieliński H.Antioxidant activity of flavone C-glucosides determined by updated analytical strategies[J].Food Chemistry,2011,124(2):672-678.
[15]Wang Nan(王楠),Tang Weimin(唐伟敏),Chu Bingquan(楚秉泉),et al.Whitening efficacy of bamboo leaf flavonoids nanoparticles based on B16 melanoma cell evaluation system[J].Fine Chemicals(精细化工),2016,33(12):1375-1380.
[16]Zhang Ying(张英),Zhang Yujing(张宇靖),Zhou Moxi(周沫希),et al.Preparation methods and application of the nanoparticle taking high purity C-glycoside flavonoids of bamboo leaf as leading material:CN201810898507.1[P].2018-08-08.
[17]Mei X,Wu Y,Mao X,et al.Antagonism of phenanthrene cytotoxicity for human embryo lung fibroblast cell line HFL-I by green tea polyphenols[J].Environmental Pollution,2011,159(1):164-168.
[18]Qiu L,Zhang M,Sturm R,et al.Inhibition of melanin synthesis by cystamine in human melanoma cells[J].Journal of Investigative Dermatology,2000,114(1):21-27.
[19]Ando H,Itoh A,Mishima Y,et al.Correlation between the number of melanosomes,tyrosinase m RNA levels,and tyrosinase activity in cultured murine melanoma cells in response to various melanogenesis regulatory agents[J].Journal of Cellular Physiology,1995,163(1):608-614.
[20]Wang N,Wu J,Li A,et al.Evaluation for whitening efficacy of cosmetics containing extract from Tricholoma matsutake Sing.[J].China Surfactant Detergent&Cosmetics,2016,(5):279-283.
[21]Martinsen O,Grimnes S,Karlsen J.Electrical methods for skin moisture assessment[J].Skin Pharmacology and Physiology,1995,8(5):237-245.
[22]Clarys P,Alewaeters K,Lambrecht R,et al.Skin color measurements:Comparison between three instruments:The chromameter,the derma spectrometer and the mexameter[J].Skin Research Technology,2000,6(4):230-238.
[23]Neethirajan S,Jayas D.Nanotechnology for the food and bioprocessing industries[J].Food Bioprocess Technology,2011,4(1):39-47.
[24]Zhang Jianyou(张建友).Studies on composition and bioactivity of water-phase extract from bamboo shavings[D].Hangzhou:Zhejiang University(浙江大学),2010.
[25]Yoshimura K,Harii K,Masuda Y,et al.Usefulness of a narrow-band reflectance spectrophotometer in evaluating effects of depigmenting treatment[J].Aesthetic Plastic Surgery,2001,25(2):129-133.