L-鸟氨酸发酵液的脱色研究
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摘要
为了提高L-鸟氨酸发酵液的脱色效率,对其脱色工艺开展研究。首先通过树脂的选择性吸附确定L-鸟氨酸发酵液中色素的化学性质,随后结合颗粒活性炭的物理结构和零电荷点分析,优选出在接近中性条件下具有优良脱色性能的颗粒活性炭。在静态条件下考察pH、温度、时间和活性炭用量等因素对其脱色性能的影响。在此基础上,考察活性炭层析柱对L-鸟氨酸发酵液的动态脱色工艺和基于两步解吸法的活性炭再生工艺,单柱可动态脱色处理45倍床层体积(BV)的发酵液,脱色率达97%以上,脱色液呈无色透明状,L-鸟氨酸损失率低于1%,活性炭再生效果保持稳定。
To improve the efficiency of decoloring L-ornithine fermentation broth,the condition of decolorization was studied.The chemical property of pigments in fermentation broth was confirmed by macroporous resin adsorption.With the analyses of physical structures and zero charge points of activated carbons,the activated carbon with excellent decolorization capacity under approximate neutral condition was selected.Then,the effects of pH,temperature,time and activated carbon dosage on decolorization under static conditions were discussed.On this basis,the continuous decolorization of L-ornithine fermentation broth with an activated carbon column and the regeneration of activated carbon using two-step desorption were studied.The fermentation broth with 45 BV was processed with the activated carbon column,the decolorization efficiency was more than 97%,the decolored solution was almost colorless,and the loss ratio of L-ornithine was less than 1 %.The regenerative effect of activated carbon was stable.
To improve the efficiency of decoloring L-ornithine fermentation broth,the condition of decolorization was studied.The chemical property of pigments in fermentation broth was confirmed by macroporous resin adsorption.With the analyses of physical structures and zero charge points of activated carbons,the activated carbon with excellent decolorization capacity under approximate neutral condition was selected.Then,the effects of pH,temperature,time and activated carbon dosage on decolorization under static conditions were discussed.On this basis,the continuous decolorization of L-ornithine fermentation broth with an activated carbon column and the regeneration of activated carbon using two-step desorption were studied.The fermentation broth with 45 BV was processed with the activated carbon column,the decolorization efficiency was more than 97%,the decolored solution was almost colorless,and the loss ratio of L-ornithine was less than 1 %.The regenerative effect of activated carbon was stable.
引文
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[13] 韩及华,吴其飞,徐斌,等.活性炭对玉米朊中黄色素的吸附机理研究[J].中国粮油学报,2016,31(10):98-107.
[14] 范明霞,张智.活性炭孔径分布和表面化学性质对吸附影响的研究进展[J].煤炭加工与综合利用,2011(1):49-54.
[15] 许建华,杨人隽,王嘉宝.水的特种处理[M].上海:同济大学出版社,1989.
[16] 马占有,李秉正,郭亚兵.吸附废水中芳香化合物的活性炭的研究进展[J].化学工程与装备,2010(12):135-137.
[17] 戴卫东,钱礼华,汪守建.活性炭吸附棉籽糖液中色素的工艺研究[J].食品科技,2009,34(9):213-216.
[2] 万红贵,熊洋,张建,等.L-鸟氨酸产品开发与应用进展[J].中国酿造,2013,32(1):8-12.
[3] 张媛媛,刘建忠,江文明.定向诱变谷氨酸棒杆菌13032产L-鸟氨酸的研究[J].现代食品科技,2010,26(12):1330-1333.
[4] ZHANG Y Y,BU Y F,LIU J Z.Production of L-ornithine from sucrose and molasses by Corynebacterium glutamicum[J].Folia Microbiol (Praha),2015,60(5):393-398.
[5] 王汉领.L-鸟氨酸发酵清洁生产工艺的研究[D].南京:南京工业大学,2011.
[6] TSUCHIDA T,UCHIBORI H,NISHIMOTO Y.Process and microorganism for producing L-ornithine by Corynebacterium,Brevibacterium or Athrobacter:US5188947A[P].1993-02-23.
[7] 张波.L-鸟氨酸的模拟移动床分离及母液回收工艺研究[D].南京:南京工业大学,2012.
[8] 万红贵,朱明新,吴启赐,等.纳滤在L -鸟氨酸后提取中的应用研究[J].膜科学与技术,2010,30(1):82-85.
[9] XU S,HAO N,XU L,et al.Series fermentation production of ornithine and succinic acid from cane molasses by Corynebacterium glutamicum [J].Biochem Eng J,2015,99:177-182.
[10] 石玲玲.大孔树脂对灰黄霉素提取液中色素的吸附脱色[D].呼和浩特:内蒙古工业大学,2007.
[11] 范延臻,王宝贞,王琳,等.改性活性炭的表面特性及其对金属离子的吸附性能[J].环境化学,2001,20(5):437-443.
[12] 杨强,李新华,林子木.银杏果多糖树脂脱色工艺[J].食品与发酵工业,2012,38(12):107-110.
[13] 韩及华,吴其飞,徐斌,等.活性炭对玉米朊中黄色素的吸附机理研究[J].中国粮油学报,2016,31(10):98-107.
[14] 范明霞,张智.活性炭孔径分布和表面化学性质对吸附影响的研究进展[J].煤炭加工与综合利用,2011(1):49-54.
[15] 许建华,杨人隽,王嘉宝.水的特种处理[M].上海:同济大学出版社,1989.
[16] 马占有,李秉正,郭亚兵.吸附废水中芳香化合物的活性炭的研究进展[J].化学工程与装备,2010(12):135-137.
[17] 戴卫东,钱礼华,汪守建.活性炭吸附棉籽糖液中色素的工艺研究[J].食品科技,2009,34(9):213-216.