基于InDel标记的大白菜育种材料分子身份证构建
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摘要
以105份大白菜育种材料为试材,精选20对均匀分布于大白菜基因组、且只能检测出2个等位基因的共显性InDel标记引物进行分子标记检测,构建分子身份证。结果表明:20对引物中有16对引物在所有材料中均扩增出单一条带,另外各有2对引物在部分材料中存在缺失和加倍现象。将所有标记引物按其所处染色体编号由小(A01)到大(A10)排列,同一染色体上的标记则按照物理位置从小到大排列,按顺序对标记赋值,构建了105份大白菜材料的分子身份证。所筛选的20对引物对未知新种质的身份证构建具有通用性和兼容性,表明InDel标记技术可以作为大白菜鉴定和分子身份证构建的有效技术手段。
Taking 105 Chinese cabbage [Brassica campestris L. ssp. pekinensis (Lour) Olsson] breeding lines as test material,this paper screened out 20 pairs distributed evenly in Chinese cabbage genome,conducted molecular marker detection on the codominant InDel primers only produced 2 alleles,and built molecular identity cards.The results showed that 16 out of 20 pairs of primers produced single band,respectively. While,2 pairs of primers each from partial materials were found loss and duplicated.All primers were ordered according to chromosome number,starting from A01 to A10 and primers on the same chromosome were ordered according to their physical position from small to large.According to these order,values were assigned to tags and 105 molecular identity cards of Chinese cabbage material were built.The screened out 20 pairs of primers had universality and compatibility for the identity cards construction of the unknown new germplasm resources,indicating that InDel marker technology could be used as an effective means for Chinese cabbage identification and molecular ID construction.
Taking 105 Chinese cabbage [Brassica campestris L. ssp. pekinensis (Lour) Olsson] breeding lines as test material,this paper screened out 20 pairs distributed evenly in Chinese cabbage genome,conducted molecular marker detection on the codominant InDel primers only produced 2 alleles,and built molecular identity cards.The results showed that 16 out of 20 pairs of primers produced single band,respectively. While,2 pairs of primers each from partial materials were found loss and duplicated.All primers were ordered according to chromosome number,starting from A01 to A10 and primers on the same chromosome were ordered according to their physical position from small to large.According to these order,values were assigned to tags and 105 molecular identity cards of Chinese cabbage material were built.The screened out 20 pairs of primers had universality and compatibility for the identity cards construction of the unknown new germplasm resources,indicating that InDel marker technology could be used as an effective means for Chinese cabbage identification and molecular ID construction.
引文
丁俊杰,刘春燕,姜翠兰,胡国华,顾鑫,杨晓贺,陈庆山.2012.利用与大豆灰斑病抗性基因连锁的SSR标记构建大豆品种(系)的分子身份证.作物学报,38(12):2206-2216.
高源,刘凤之,王昆,王大江,龚欣,刘立军.2015.苹果部分种质资源分子身份证的构建.中国农业科学,48(19):3887-3898.
辜大川,邱东峰,殷明珠,焦春海,杨金松,张再君,刘春萍.2012.水稻优异资源分子身份证的建立.湖北农业科学,51(24):5579-5589.
胡振帮,高运来,齐照明,蒋洪蔚,刘春燕,辛大伟,胡国华,潘校成,陈庆山.2016.作物分子身份证构建软件ID analysis的编制.中国农业科学,49(12):2255-2266.
李国强.2008.大白菜核心种质的构建与评价[硕士论文].北京:中国农业科学院.
刘栓桃,张志刚,李巧云,王淑芬,卢金东,张晓燕,徐文玲,刘贤娴,付卫民,赵智中.2014.大白菜e IF(iso)4E.a假基因突变体的鉴别及相关检测标记的开发.中国农学通报,30(4):101-107.
刘栓桃,张志刚,李巧云,赵智中,王立华,王淑芬,徐文玲,刘贤娴,刘辰.2017.大白菜重组自交系群体构建过程中剩余杂合位点的变化趋势.分子植物育种,15(7):2730-2735.
刘栓桃,张志刚,司立英,王荣花,李巧云,王立华,赵智中,梁水美,张全芳,步迅.2018.基于In Dels标记的大白菜育种材料的亲缘关系鉴定.植物遗传资源学报,19(4):657-667.
陆徐忠,倪金龙,李莉,汪秀峰,马卉,张小娟,杨剑波.2014.利用SSR分子指纹和商品信息构建水稻品种身份证.作物学报,40(5):823-829.
邱杨,李锡香,李清霞,陈亦辰,沈镝,王海平,宋江萍.2014.利用SSR标记构建萝卜种质资源分子身份证.植物遗传资源学报,15(3):648-654.
谭其猛.1979.试论大白菜品种的起源、分布和演化.中国农业科学,12(4):68-75.
唐玉娟,宋恩亮,王梓钰,马启彬,年海.2016.广东夏大豆新品种(系)分子身份证的构建.广东农业科学,43(5):37-41.
万映伶,张嘉,刘爱青,张孔英,刘燕.2018.基于SSR标记构建中国芍药品种资源分子身份证.西北农林科技大学学报:自然科学版,46(12):1-8.
魏利斌,苗红梅,李春,段迎辉,徐芳芳,张海洋.2017.芝麻SNP和In Del标记遗传多样性、群体结构及连锁不平衡分析.分子植物育种,15(8):3070-3079.
徐安然,付永平,王延锋,张志武,纪策,王菲,叶健强,李玉,宋冰.2017.黑木耳部分种质资源SSR分子身份证的构建.农业生物技术学报,25(12):1930-1939.
薛华柏,赵瑞娟,王磊,杨健,王龙,王苏珂,苏艳丽,李秀根.2018.梨品种SSR特征指纹图谱与分子身份证构建.中国南方果树,47(s):42-50.
杨金雪.2013.用于大白菜品种核酸指纹库构建的SSR核心引物筛选[硕士论文].扬州:扬州大学.
张志刚,赵智中,李巧云,王晓,刘栓桃,王淑芬,徐文玲,刘贤娴,刘辰.2016.大白菜In Dels标记开发及其在剩余杂合体鉴定中的应用.农业生物技术学报,24(4):510-518.
郑海燕.2010.利用RAPD、ISSR和SRAP标记技术构建红麻(Hibiscus cannabinus L.)种质资源分子身份证[硕士论文].北京:中国农业科学院.
朱东旭,王彦华,赵建军,刘博李,晓峰轩,淑欣,申书兴.2014.结球甘蓝相对于大白菜连锁群特异In Del标记的建立及应用.园艺学报,41(8):1699-1706.
Cheng F,MandákováT,Wu J,Xie Q,Lysak M A,Wang X.2013.Deciphering the diploid ancestral genome of the mesohexaploid Brassica rapa.Plant Cell,25(5):1541-1554.
高源,刘凤之,王昆,王大江,龚欣,刘立军.2015.苹果部分种质资源分子身份证的构建.中国农业科学,48(19):3887-3898.
辜大川,邱东峰,殷明珠,焦春海,杨金松,张再君,刘春萍.2012.水稻优异资源分子身份证的建立.湖北农业科学,51(24):5579-5589.
胡振帮,高运来,齐照明,蒋洪蔚,刘春燕,辛大伟,胡国华,潘校成,陈庆山.2016.作物分子身份证构建软件ID analysis的编制.中国农业科学,49(12):2255-2266.
李国强.2008.大白菜核心种质的构建与评价[硕士论文].北京:中国农业科学院.
刘栓桃,张志刚,李巧云,王淑芬,卢金东,张晓燕,徐文玲,刘贤娴,付卫民,赵智中.2014.大白菜e IF(iso)4E.a假基因突变体的鉴别及相关检测标记的开发.中国农学通报,30(4):101-107.
刘栓桃,张志刚,李巧云,赵智中,王立华,王淑芬,徐文玲,刘贤娴,刘辰.2017.大白菜重组自交系群体构建过程中剩余杂合位点的变化趋势.分子植物育种,15(7):2730-2735.
刘栓桃,张志刚,司立英,王荣花,李巧云,王立华,赵智中,梁水美,张全芳,步迅.2018.基于In Dels标记的大白菜育种材料的亲缘关系鉴定.植物遗传资源学报,19(4):657-667.
陆徐忠,倪金龙,李莉,汪秀峰,马卉,张小娟,杨剑波.2014.利用SSR分子指纹和商品信息构建水稻品种身份证.作物学报,40(5):823-829.
邱杨,李锡香,李清霞,陈亦辰,沈镝,王海平,宋江萍.2014.利用SSR标记构建萝卜种质资源分子身份证.植物遗传资源学报,15(3):648-654.
谭其猛.1979.试论大白菜品种的起源、分布和演化.中国农业科学,12(4):68-75.
唐玉娟,宋恩亮,王梓钰,马启彬,年海.2016.广东夏大豆新品种(系)分子身份证的构建.广东农业科学,43(5):37-41.
万映伶,张嘉,刘爱青,张孔英,刘燕.2018.基于SSR标记构建中国芍药品种资源分子身份证.西北农林科技大学学报:自然科学版,46(12):1-8.
魏利斌,苗红梅,李春,段迎辉,徐芳芳,张海洋.2017.芝麻SNP和In Del标记遗传多样性、群体结构及连锁不平衡分析.分子植物育种,15(8):3070-3079.
徐安然,付永平,王延锋,张志武,纪策,王菲,叶健强,李玉,宋冰.2017.黑木耳部分种质资源SSR分子身份证的构建.农业生物技术学报,25(12):1930-1939.
薛华柏,赵瑞娟,王磊,杨健,王龙,王苏珂,苏艳丽,李秀根.2018.梨品种SSR特征指纹图谱与分子身份证构建.中国南方果树,47(s):42-50.
杨金雪.2013.用于大白菜品种核酸指纹库构建的SSR核心引物筛选[硕士论文].扬州:扬州大学.
张志刚,赵智中,李巧云,王晓,刘栓桃,王淑芬,徐文玲,刘贤娴,刘辰.2016.大白菜In Dels标记开发及其在剩余杂合体鉴定中的应用.农业生物技术学报,24(4):510-518.
郑海燕.2010.利用RAPD、ISSR和SRAP标记技术构建红麻(Hibiscus cannabinus L.)种质资源分子身份证[硕士论文].北京:中国农业科学院.
朱东旭,王彦华,赵建军,刘博李,晓峰轩,淑欣,申书兴.2014.结球甘蓝相对于大白菜连锁群特异In Del标记的建立及应用.园艺学报,41(8):1699-1706.
Cheng F,MandákováT,Wu J,Xie Q,Lysak M A,Wang X.2013.Deciphering the diploid ancestral genome of the mesohexaploid Brassica rapa.Plant Cell,25(5):1541-1554.