Akt、NF-κB、p65、ERK1/2和p38与乳腺炎患者病情发展的相关性研究
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摘要
目的探讨Akt、NF-κB、p65、ERK1/2和p38与乳腺炎患者病情发展的相关性。方法 95例乳腺炎患者根据患者病情严重程分为乳腺炎初期组(n=31)、乳腺炎脓肿期组(n=23)。观察3组患者Akt、NF-κB、P65、ERK1/2和P38的水平。结果乳腺炎脓肿期组P65、NF-κB表达水平明显高于乳腺炎初期组、乳腺炎中期组(P<0.05)。乳腺炎中期组P65、NF-κB水平明显高于乳腺炎初期组(P<0.05)。乳腺炎脓肿期组其Akt、ERK1/2和p38阳性评分明显高于乳腺炎初期组、乳腺炎中期组,差异有统计学意义(P<0.05)。结论 ERK1/2蛋白、p38丝裂原活化蛋白激酶、NF-κB、NF-κB p65以及Akt的阳性表达率越高乳腺炎病情越加严重。值得临床重视。
Objective To investigate the correlation of Akt, NF-κB, p65, ERK1/2 and p38 to disease progression of patients with mastitis.Methods Ninety-five patients with mastitis were divided into initial-stage mastitis group(n=31) and mastitis and breast abscess group(n=23) according to the severity of the patient's condition. The levels of Akt, NF-κB, P65, ERK1/2 and P38 were observed and compared among the three groups.Results The expression levels of P65 and NF-κB in the mastitis and breast abscess group were significantly higher than those in the initial-stage mastitis group and the intermediate-stage mastitis group(P<0.05). The levels of P65 and NF-κB in intermediate-stage mastitis group were significantly higher than those in the initial-stage mastitis group(P<0.05). The positive scores of Akt, ERK1/2 and p38 in the mastitis and breast abscess group were significantly higher than those in the initial-stage mastitis group and the intermediate-stage mastitis group, and the difference was statistically significant(P<0.05).Conclusion The higher the positive expression rate of ERK1/2 protein, p38 mitogen-activated protein kinase, NF-κB, NF-κB p65 and Akt, the more serious the condition of mastitis. Therefore, it is worthy of clinical attention.
Objective To investigate the correlation of Akt, NF-κB, p65, ERK1/2 and p38 to disease progression of patients with mastitis.Methods Ninety-five patients with mastitis were divided into initial-stage mastitis group(n=31) and mastitis and breast abscess group(n=23) according to the severity of the patient's condition. The levels of Akt, NF-κB, P65, ERK1/2 and P38 were observed and compared among the three groups.Results The expression levels of P65 and NF-κB in the mastitis and breast abscess group were significantly higher than those in the initial-stage mastitis group and the intermediate-stage mastitis group(P<0.05). The levels of P65 and NF-κB in intermediate-stage mastitis group were significantly higher than those in the initial-stage mastitis group(P<0.05). The positive scores of Akt, ERK1/2 and p38 in the mastitis and breast abscess group were significantly higher than those in the initial-stage mastitis group and the intermediate-stage mastitis group, and the difference was statistically significant(P<0.05).Conclusion The higher the positive expression rate of ERK1/2 protein, p38 mitogen-activated protein kinase, NF-κB, NF-κB p65 and Akt, the more serious the condition of mastitis. Therefore, it is worthy of clinical attention.
引文
1 王世民,刘孝兵.带状疱疹伴发后遗神经痛患者外周血p38MAPK、ERK1/2、JNK表达量与细胞因子、疼痛介质的相关性.海南医学院学报,2017,23:2510-2513.
2 周丽峰,牛俊坤,缪应雷,等.干扰和过表达热休克转录因子2对肠上皮细胞炎症的影响.西部医学,2017,29:308-315.
3 曾泽,何欢,任玉鹏,等.rfaE基因通过MAPKs/NF-κB信号通路对副猪嗜血杆菌LOS诱导猪肺泡巨噬细胞炎症反应中作用的研究.畜牧兽医学报,2016,47:978-984.
4 潘建科,曹学伟,刘军,等.龙鳖胶囊对骨关节炎软骨细胞p38MAPK信号通路及NF-κB p65的影响.中国组织工程研究,2016,20:6868-6877.
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6 王凤华,韩吉春,李德芳,等.霍山石斛水提取物通过介导NF-κB/p65和p38 MAPK减轻小鼠酒精性肝损伤.天然产物研究与开发,2017:569-574.
7 任倩倩,汪丽佩,赵炜,等.甘草查尔酮A抑制ERK1/2/NF-κB途径减轻吸烟诱导的小鼠急性肺损伤.中国药理学通报,2016,32:643-651.
8 李文鹏,赵高炀,杨薛康.钠氢交换蛋白1抑制剂对烧伤脓毒症大鼠肠道损伤的作用及其机制.中华烧伤杂志,2017,33:349-354.
9 赵慧娜,陈自喜,古航,等.临产前后子宫肌收缩相关蛋白在人子宫肌中的表达及相关信号转导途径的研究.临床和实验医学杂志,2016,15:2281-2284.
10 王世伟,徐宁.山茱萸环烯醚萜总苷对小鼠胰岛素抵抗和非酒精性肝损伤的改善作用及其机制研究.天津中医药,2017,34:195-199.
11 刘清,丁光仁,惠永峰,等.HMGB1及其受体RAGE分子信号通路参与调节肝细胞肝癌增殖和侵袭迁移机制的研究.临床和实验医学杂志,2017,16:213-217.
12 夏龙飞,巨红妹,周红,等.mTOR活化对抗β_2GPI抗体/β_2GPI复合物诱导THP-1细胞组织因子表达的影响.临床检验杂志,2017,35:215-220.
13 马胜辉,李建华,王翔,等.胃低分化腺癌患者中P53、KI-67、EGFR与HER-2的表达水平及其与预后的相关性.中国医药导刊,2016,18:188-190.
14 李文军,康红兵,杨立,等.延龄草皂苷通过NF-κB/MAPK信号通路调控MMPs表达并抑制骨肉瘤Saos-2细胞的迁移和侵袭.肿瘤,2016,36:968-976.
15 吴标良,唐乾利,覃晓洁,等.基于MEBT/MEBO的ERK1/2和p38信号通路与糖尿病足免疫表达关系的研究.重庆医学,2016,45:5069-5071.
16 朱芹英,张卉,杨铁骊.桂枝提取物对动脉粥样硬化大鼠白细胞介素6/信号转导子和转录活化子3信号通路的影响.中国现代医学杂志,2016,26:11-15.
17 陈雄,虞伟慧,龚小花,等.厚朴酚通过MAPK/NF-κB信号通路改善1型糖尿病模型小鼠的心肌损伤.中草药,2017,48:4719-4725.
18 栗薇,郭姗姗,包蕾,等.小儿感冒宁颗粒对流感病毒感染后小鼠血清中MCP-1含量及肺组织中ERK和NF-κB信号通路的影响.中国药物警戒,2017,14:65-68.
2 周丽峰,牛俊坤,缪应雷,等.干扰和过表达热休克转录因子2对肠上皮细胞炎症的影响.西部医学,2017,29:308-315.
3 曾泽,何欢,任玉鹏,等.rfaE基因通过MAPKs/NF-κB信号通路对副猪嗜血杆菌LOS诱导猪肺泡巨噬细胞炎症反应中作用的研究.畜牧兽医学报,2016,47:978-984.
4 潘建科,曹学伟,刘军,等.龙鳖胶囊对骨关节炎软骨细胞p38MAPK信号通路及NF-κB p65的影响.中国组织工程研究,2016,20:6868-6877.
5 原庆会,何伟,王安群,等.乳腺癌组织中 ER、PR、HER-2、BRCA-1的表达情况及与远期预后的关系.医学综述,2015,7:722-723.
6 王凤华,韩吉春,李德芳,等.霍山石斛水提取物通过介导NF-κB/p65和p38 MAPK减轻小鼠酒精性肝损伤.天然产物研究与开发,2017:569-574.
7 任倩倩,汪丽佩,赵炜,等.甘草查尔酮A抑制ERK1/2/NF-κB途径减轻吸烟诱导的小鼠急性肺损伤.中国药理学通报,2016,32:643-651.
8 李文鹏,赵高炀,杨薛康.钠氢交换蛋白1抑制剂对烧伤脓毒症大鼠肠道损伤的作用及其机制.中华烧伤杂志,2017,33:349-354.
9 赵慧娜,陈自喜,古航,等.临产前后子宫肌收缩相关蛋白在人子宫肌中的表达及相关信号转导途径的研究.临床和实验医学杂志,2016,15:2281-2284.
10 王世伟,徐宁.山茱萸环烯醚萜总苷对小鼠胰岛素抵抗和非酒精性肝损伤的改善作用及其机制研究.天津中医药,2017,34:195-199.
11 刘清,丁光仁,惠永峰,等.HMGB1及其受体RAGE分子信号通路参与调节肝细胞肝癌增殖和侵袭迁移机制的研究.临床和实验医学杂志,2017,16:213-217.
12 夏龙飞,巨红妹,周红,等.mTOR活化对抗β_2GPI抗体/β_2GPI复合物诱导THP-1细胞组织因子表达的影响.临床检验杂志,2017,35:215-220.
13 马胜辉,李建华,王翔,等.胃低分化腺癌患者中P53、KI-67、EGFR与HER-2的表达水平及其与预后的相关性.中国医药导刊,2016,18:188-190.
14 李文军,康红兵,杨立,等.延龄草皂苷通过NF-κB/MAPK信号通路调控MMPs表达并抑制骨肉瘤Saos-2细胞的迁移和侵袭.肿瘤,2016,36:968-976.
15 吴标良,唐乾利,覃晓洁,等.基于MEBT/MEBO的ERK1/2和p38信号通路与糖尿病足免疫表达关系的研究.重庆医学,2016,45:5069-5071.
16 朱芹英,张卉,杨铁骊.桂枝提取物对动脉粥样硬化大鼠白细胞介素6/信号转导子和转录活化子3信号通路的影响.中国现代医学杂志,2016,26:11-15.
17 陈雄,虞伟慧,龚小花,等.厚朴酚通过MAPK/NF-κB信号通路改善1型糖尿病模型小鼠的心肌损伤.中草药,2017,48:4719-4725.
18 栗薇,郭姗姗,包蕾,等.小儿感冒宁颗粒对流感病毒感染后小鼠血清中MCP-1含量及肺组织中ERK和NF-κB信号通路的影响.中国药物警戒,2017,14:65-68.