马铃薯种质资源对晚疫病的室内及田间抗性鉴定
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摘要
【目的】明确马铃薯种质资源对晚疫病的抗感性,为在江西省选育推广和合理布局抗病马铃薯品种及对晚疫病进行综合治理提供科学依据。【方法】采用室内离体叶片接种法和田间自然病圃法对35份马铃薯种质材料进行晚疫病抗性鉴定,综合评价参试材料的抗感性。【结果】参试材料中,费乌瑞它、中薯3号、中薯10号、中薯13号、08HE042-2、N157、N108和D692等8份材料在两种试验方法中均表现为高感,而中薯17号、07HE147-4、07HE005-06、D516、07HE169-1和D504等6份材料表现为高抗或抗病。聚类分析结果表明,除08CA0687、中薯5号、中薯11号和D545等4份材料在两种方法中的鉴定结果存在差异外,其余材料的鉴定结果基本一致。【结论】马铃薯离体叶片对晚疫病的抗病性可基本代表成株期的抗病性。07HE147-4、07HE005-06、D516、07HE169-1和D504等高抗或抗病材料可作为马铃薯抗病育种的亲本材料,华薯3号、中薯7号和中薯17号等高抗至中抗晚疫病可在江西省内推广种植。
【Objective】The resistance of potato germplasm to late blight was studied to provide scientific basis for breeding and promoting disease-resistant potato varieties and carrying out comprehensive treatment of late blight in Jiangxi.【Method】Inoculation of detached leaves indoor and natural infection in field were applied to identifying the resistance of 35 potato materials to late blight and evaluate the resistance of the tested materials comprehensively.【Result】Among the tested materials,eight materials(Favorita,Zhongshu 3,Zhongshu 10,Zhongshu 13,08 HE042-2,N157,N108 and D692)were highly susceptible to late blight,and six materials(Zhongshu 17,07 HE147-4,07 HE005-06,D516,07 HE169-1 and D504)showed high resistance or resistance to late blight in both methods. Cluster analysis showed that the identification results of all the materials were relatively consistent in the two methods except for four materials(08 CA0687,Zhongshu 5,Zhongshu 11 and D545).【Conclusion】The resistance of detached potato leaves to late blight can basically represent the resistance of potato in adult period. The materials(07 HE147-4,07 HE005-06,D516,07 HE169-1 and D504)with high resistance can be parent material to breed disease resistant potato. Materials with highly resistance to late blight such as Huashu 3,Zhongshu 7 and Zhongshu 17 can be planted widely in Jiangxi.
【Objective】The resistance of potato germplasm to late blight was studied to provide scientific basis for breeding and promoting disease-resistant potato varieties and carrying out comprehensive treatment of late blight in Jiangxi.【Method】Inoculation of detached leaves indoor and natural infection in field were applied to identifying the resistance of 35 potato materials to late blight and evaluate the resistance of the tested materials comprehensively.【Result】Among the tested materials,eight materials(Favorita,Zhongshu 3,Zhongshu 10,Zhongshu 13,08 HE042-2,N157,N108 and D692)were highly susceptible to late blight,and six materials(Zhongshu 17,07 HE147-4,07 HE005-06,D516,07 HE169-1 and D504)showed high resistance or resistance to late blight in both methods. Cluster analysis showed that the identification results of all the materials were relatively consistent in the two methods except for four materials(08 CA0687,Zhongshu 5,Zhongshu 11 and D545).【Conclusion】The resistance of detached potato leaves to late blight can basically represent the resistance of potato in adult period. The materials(07 HE147-4,07 HE005-06,D516,07 HE169-1 and D504)with high resistance can be parent material to breed disease resistant potato. Materials with highly resistance to late blight such as Huashu 3,Zhongshu 7 and Zhongshu 17 can be planted widely in Jiangxi.
引文
方树民,翁定河,徐大东,许长敏.2001.马铃薯品种对晚疫病的抗性评价[J].福建农业科技,(4):5-6.[Fang S M,Weng D H,Xu D D,Xu C M.2001.Resistance evaluation of potato varieties to late blight[J].Fujian Agricultural Science and Technology,(4):5-6.]
冯明义,王炜,姜府文,阳勇,吴石平.2016.贵州春播一熟区马铃薯品种(系)对晚疫病抗性田间评价[J].种子,35(12):78-82.[Feng M Y,Wang W,Jiang F W,Yang Y,Wu S P.2016.Evaluation of potato cultivars with late blight resistance in spring seeding single cropping region in Guizhou[J].Seed,35(12):78-82.]
刘浩,何建国,朱玉斌,沈瑞清.2012.宁夏固原市马铃薯主栽品种对晚疫病的抗性[J].河南农业科学,41(4):97-99.[Liu H,He J G,Zhu Y B,Shen R Q.2012.Resistance of main potato cultivars to late blight in Guyuan City,Ningxia Province[J].Journal of Henan Agricultural Sciences,41(4):97-99.]
娄树宝,李庆全,田国奎,徐洪岩,王海燕,王立春,盛万民.2012.马铃薯种质资源晚疫病抗性鉴定与评价[J].黑龙江农业科学,(12):11-13.[Lou S B,Li Q Q,Tian G K,Xu H Y,Wang H Y,Wang L C,Sheng W M.2012.Identification and evaluation for resistance of potato germplasm to late blight[J].Heilongjiang Agricultural Sciences,(12):11-13.]
卢丽丽,曹继芬,潘哲超,刘凌云,赵志坚,杨琼芬.2016.云南省马铃薯主要品种晚疫病抗性评价[J].中国马铃薯,30(6):356-361.[Lu L L,Cao J F,Pan Z C,Liu L Y,Zhao Z J,Yang Q F.2016.Resistance evaluation of main potato varieties to late blight in Yunnan Province[J].Chinese Potato Journal,30(6):356-361.]
鲁绍凤,杨艳丽,胡先奇,罗文富,李丽娟.2006.云南省马铃薯主栽品种对晚疫病的抗性分析[J].云南农业大学学报,21(5):586-590.[Lu S F,Yang Y L,Hu X Q,Luo W F,Li L J.2006.Analysis of resistance to potato late blight in Yunnan Province[J].Journal of Yunnan Agricultural University,21(5):586-590.]
罗文忠,毛国军,杨晓风,李蔚传,陈恩发.2015.大方县马铃薯主栽品种晚疫病的抗性监测[J].安徽农业科学,43(35):22-23.[Luo W Z,Mao G J,Yang X F,Li W C,Chen E F.2015.Resistance monitoring of late blight disease of mainly-planting cultivars in Dafang County[J].Journal of Anhui Agricultural Sciences,43(35):22-23.]
罗彦涛,孟润杰,赵建江,韩秀英,马志强,王文桥,张小风.2016.马铃薯晚疫病菌对氟吡菌胺抗性突变体的获得及其生物学性状[J].中国农业科学,49(19):3733-3745.[Luo Y T,Meng R J,Zhao J J,Han X Y,Ma Z Q,Wang W Q,Zhang X F.2016.Acquisition and biological characteristics of fluopicolide-resistant isolates in Phytophthora infestans[J].Scientia Agricultura Sinica,49(19):3733-3745.]
娜仁,张笑宇,张之为,杜衍平,赵君.2010.马铃薯不同品种(系)对晚疫病抗性鉴定[J].作物杂志,(4):59-61.[Na R,Zhang X Y,Zhang Z W,Du Y P,Zhao J.2010.Evaluation on resistance of different potato cultivars(lines)to Phytophthora infestans[J].Crops,(4):59-61.]
宋伯符,谢开云.1997.CIP的全球晚疫病防治倡议与我国的参与[J].中国马铃薯,11(1):51-55.[Song B F,Xie KY.1997.The global initiative to prevent and control late blight by CIP and China’s participation[J].Chinese Potato Journal,11(1):51-55.]
万安琪,蒋继志,沙海天,李颖,王蓓蓓,金鑫,张亚辉.2017.拮抗细菌W-7的鉴定及其对致病疫霉的抑制作用[J].河南农业科学,46(2):55-59.[Wan A Q,Jiang J Z,Sha HT,Li Y,Wang B B,Jin X,Zhang Y H.2017.Identification of antagonistic bacterium W-7 strain and its inhibition on Phytophthora infestans[J].Journal of Henan A-gricultural Sciences,46(2):55-59.]
王鹏,李芳弟,郭天顺,何二良,颉炜清,齐小东,吕汰.2014.马铃薯品种(系)晚疫病抗性鉴定[J].中国马铃薯,28(5):264-269.[Wang P,Li F D,Guo T S,He E L,Jie W Q,Qi X D,LüT.2014.Identification of potato varieties(clones)for late blight resistance[J].Chinese Potato Journal,28(5):264-269.]
王蓉,肖卫平,韦青,邓忠国.2013.不同马铃薯品种对晚疫病的田间抗性比较试验[J].长江蔬菜,(14):24-27.[Wang R,Xiao W P,Wei Q,Deng Z G.2013.Resistance comparison of different potato varieties to late blight in field[J].Journal of Changjiang Vegetables,(14):24-27.]
王信,程亮,郭青云.2011.青海省马铃薯品种对晚疫病菌的抗性分析[J].中国马铃薯,25(4):238-241.[Wang X,Cheng L,Guo Q Y.2011.Analysis for resistance of potato cultivars to Phytophthora infestans in Qinghai Province[J].Chinese Potato Journal,25(4):238-241.]
杨丽娜,段国华,覃雁瑜,祝雯,潘哲超,隋启君,詹家馁.2016.2012年云南省会泽县马铃薯晚疫病菌小种结构分析[J].热带作物学报,37(1):158-163.[Yang L N,Duan G H,Qin Y Y,Zhu W,Pan Z C,Sui Q J,Zhan J N.2016.Race structure of Phytophthora infestans population collected from Huize,Yunnan in 2012[J].Chinese Journal of Tropical Crops,37(1):158-163.]
杨胜先,张绍荣,龙国,聂绍科.2015.贵州省马铃薯晚疫病菌生理小种的组成与分布[J].南方农业学报,46(4):597-601.[Yang S X,Zhang S R,Long G,Nie S K.2015.Composition and distribution of physiological race of Phytophthora infestans in Guizhou[J].Journal of Southern Agriculture,46(4):597-601.]
杨艳丽,胡先奇,鲁绍凤,罗文富,Ryu K Y,肖浪涛.2007.云南省马铃薯晚疫病菌生理小种的组成与分布[J].华中农业大学学报,36(3):297-301.[Yang Y L,Hu X Q,Lu S F,Luo W F,Ryu K Y,Xiao L T.2007.Composition and distribution of physiological race of Phytophthora infestans in Yunnan Province[J].Journal of Huazhong Agricultural University,36(3):297-301.]
姚裕琪,巩秀峰,高奇华,曹亚利,赵敬明.2001.马铃薯晚疫病抗性鉴定及评价[J].内蒙古农业科技,(2):8-9.[Yao Y Q,Gong X F,Gao Q H,Cao Y L,Zhao J M.2001.Resistance identification and evaluation for late blight[J].Inner Mongolia Agricultural Science and Technology,(2):8-9.]
詹金碧,江健,石声俊,冯雪松,刘霞,吴石平,何海永.2009.部分马铃薯主栽品种田间抗晚疫病性初步鉴定[J].贵州农业科学,37(3):47-48.[Zhan J B,Jiang J,Shi S J,Feng X S,Liu X,Wu S P,He H Y.2009.Preliminary identification of late blight resistance of some main cultivation potato varieties in field[J].Guizhou Agricultural Sciences,37(3):47-48.]
Evers D,Ghislain M,Hausman J F,Dommes J.2003.Differential gene expression in two lines differing in their resistance to Phytophthora infestans[J].Journal of Plant Physiology,160(6):709-712.
Gisi U,Cohen Y.1996.Risistance to phenylamide fungicides:A case study with Phytophthora infestans involving mating type and race structure[J].Annual Review of Phytopathology,34:549-572.
Ivanova D G,Gughova N V,Merzlyak M N,Rassadina G V.1991.Effect of Phytophthora infestans infection on superoxide dismutase dependent cytochrome C-reducing activities of leaves as related to resistance of potato plants to late blight[J].Plant Sciences,78(2):151-156.
冯明义,王炜,姜府文,阳勇,吴石平.2016.贵州春播一熟区马铃薯品种(系)对晚疫病抗性田间评价[J].种子,35(12):78-82.[Feng M Y,Wang W,Jiang F W,Yang Y,Wu S P.2016.Evaluation of potato cultivars with late blight resistance in spring seeding single cropping region in Guizhou[J].Seed,35(12):78-82.]
刘浩,何建国,朱玉斌,沈瑞清.2012.宁夏固原市马铃薯主栽品种对晚疫病的抗性[J].河南农业科学,41(4):97-99.[Liu H,He J G,Zhu Y B,Shen R Q.2012.Resistance of main potato cultivars to late blight in Guyuan City,Ningxia Province[J].Journal of Henan Agricultural Sciences,41(4):97-99.]
娄树宝,李庆全,田国奎,徐洪岩,王海燕,王立春,盛万民.2012.马铃薯种质资源晚疫病抗性鉴定与评价[J].黑龙江农业科学,(12):11-13.[Lou S B,Li Q Q,Tian G K,Xu H Y,Wang H Y,Wang L C,Sheng W M.2012.Identification and evaluation for resistance of potato germplasm to late blight[J].Heilongjiang Agricultural Sciences,(12):11-13.]
卢丽丽,曹继芬,潘哲超,刘凌云,赵志坚,杨琼芬.2016.云南省马铃薯主要品种晚疫病抗性评价[J].中国马铃薯,30(6):356-361.[Lu L L,Cao J F,Pan Z C,Liu L Y,Zhao Z J,Yang Q F.2016.Resistance evaluation of main potato varieties to late blight in Yunnan Province[J].Chinese Potato Journal,30(6):356-361.]
鲁绍凤,杨艳丽,胡先奇,罗文富,李丽娟.2006.云南省马铃薯主栽品种对晚疫病的抗性分析[J].云南农业大学学报,21(5):586-590.[Lu S F,Yang Y L,Hu X Q,Luo W F,Li L J.2006.Analysis of resistance to potato late blight in Yunnan Province[J].Journal of Yunnan Agricultural University,21(5):586-590.]
罗文忠,毛国军,杨晓风,李蔚传,陈恩发.2015.大方县马铃薯主栽品种晚疫病的抗性监测[J].安徽农业科学,43(35):22-23.[Luo W Z,Mao G J,Yang X F,Li W C,Chen E F.2015.Resistance monitoring of late blight disease of mainly-planting cultivars in Dafang County[J].Journal of Anhui Agricultural Sciences,43(35):22-23.]
罗彦涛,孟润杰,赵建江,韩秀英,马志强,王文桥,张小风.2016.马铃薯晚疫病菌对氟吡菌胺抗性突变体的获得及其生物学性状[J].中国农业科学,49(19):3733-3745.[Luo Y T,Meng R J,Zhao J J,Han X Y,Ma Z Q,Wang W Q,Zhang X F.2016.Acquisition and biological characteristics of fluopicolide-resistant isolates in Phytophthora infestans[J].Scientia Agricultura Sinica,49(19):3733-3745.]
娜仁,张笑宇,张之为,杜衍平,赵君.2010.马铃薯不同品种(系)对晚疫病抗性鉴定[J].作物杂志,(4):59-61.[Na R,Zhang X Y,Zhang Z W,Du Y P,Zhao J.2010.Evaluation on resistance of different potato cultivars(lines)to Phytophthora infestans[J].Crops,(4):59-61.]
宋伯符,谢开云.1997.CIP的全球晚疫病防治倡议与我国的参与[J].中国马铃薯,11(1):51-55.[Song B F,Xie KY.1997.The global initiative to prevent and control late blight by CIP and China’s participation[J].Chinese Potato Journal,11(1):51-55.]
万安琪,蒋继志,沙海天,李颖,王蓓蓓,金鑫,张亚辉.2017.拮抗细菌W-7的鉴定及其对致病疫霉的抑制作用[J].河南农业科学,46(2):55-59.[Wan A Q,Jiang J Z,Sha HT,Li Y,Wang B B,Jin X,Zhang Y H.2017.Identification of antagonistic bacterium W-7 strain and its inhibition on Phytophthora infestans[J].Journal of Henan A-gricultural Sciences,46(2):55-59.]
王鹏,李芳弟,郭天顺,何二良,颉炜清,齐小东,吕汰.2014.马铃薯品种(系)晚疫病抗性鉴定[J].中国马铃薯,28(5):264-269.[Wang P,Li F D,Guo T S,He E L,Jie W Q,Qi X D,LüT.2014.Identification of potato varieties(clones)for late blight resistance[J].Chinese Potato Journal,28(5):264-269.]
王蓉,肖卫平,韦青,邓忠国.2013.不同马铃薯品种对晚疫病的田间抗性比较试验[J].长江蔬菜,(14):24-27.[Wang R,Xiao W P,Wei Q,Deng Z G.2013.Resistance comparison of different potato varieties to late blight in field[J].Journal of Changjiang Vegetables,(14):24-27.]
王信,程亮,郭青云.2011.青海省马铃薯品种对晚疫病菌的抗性分析[J].中国马铃薯,25(4):238-241.[Wang X,Cheng L,Guo Q Y.2011.Analysis for resistance of potato cultivars to Phytophthora infestans in Qinghai Province[J].Chinese Potato Journal,25(4):238-241.]
杨丽娜,段国华,覃雁瑜,祝雯,潘哲超,隋启君,詹家馁.2016.2012年云南省会泽县马铃薯晚疫病菌小种结构分析[J].热带作物学报,37(1):158-163.[Yang L N,Duan G H,Qin Y Y,Zhu W,Pan Z C,Sui Q J,Zhan J N.2016.Race structure of Phytophthora infestans population collected from Huize,Yunnan in 2012[J].Chinese Journal of Tropical Crops,37(1):158-163.]
杨胜先,张绍荣,龙国,聂绍科.2015.贵州省马铃薯晚疫病菌生理小种的组成与分布[J].南方农业学报,46(4):597-601.[Yang S X,Zhang S R,Long G,Nie S K.2015.Composition and distribution of physiological race of Phytophthora infestans in Guizhou[J].Journal of Southern Agriculture,46(4):597-601.]
杨艳丽,胡先奇,鲁绍凤,罗文富,Ryu K Y,肖浪涛.2007.云南省马铃薯晚疫病菌生理小种的组成与分布[J].华中农业大学学报,36(3):297-301.[Yang Y L,Hu X Q,Lu S F,Luo W F,Ryu K Y,Xiao L T.2007.Composition and distribution of physiological race of Phytophthora infestans in Yunnan Province[J].Journal of Huazhong Agricultural University,36(3):297-301.]
姚裕琪,巩秀峰,高奇华,曹亚利,赵敬明.2001.马铃薯晚疫病抗性鉴定及评价[J].内蒙古农业科技,(2):8-9.[Yao Y Q,Gong X F,Gao Q H,Cao Y L,Zhao J M.2001.Resistance identification and evaluation for late blight[J].Inner Mongolia Agricultural Science and Technology,(2):8-9.]
詹金碧,江健,石声俊,冯雪松,刘霞,吴石平,何海永.2009.部分马铃薯主栽品种田间抗晚疫病性初步鉴定[J].贵州农业科学,37(3):47-48.[Zhan J B,Jiang J,Shi S J,Feng X S,Liu X,Wu S P,He H Y.2009.Preliminary identification of late blight resistance of some main cultivation potato varieties in field[J].Guizhou Agricultural Sciences,37(3):47-48.]
Evers D,Ghislain M,Hausman J F,Dommes J.2003.Differential gene expression in two lines differing in their resistance to Phytophthora infestans[J].Journal of Plant Physiology,160(6):709-712.
Gisi U,Cohen Y.1996.Risistance to phenylamide fungicides:A case study with Phytophthora infestans involving mating type and race structure[J].Annual Review of Phytopathology,34:549-572.
Ivanova D G,Gughova N V,Merzlyak M N,Rassadina G V.1991.Effect of Phytophthora infestans infection on superoxide dismutase dependent cytochrome C-reducing activities of leaves as related to resistance of potato plants to late blight[J].Plant Sciences,78(2):151-156.