ELISA与HPLC-MS检测玉米中黄曲霉毒素B_1的比较分析
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摘要
本实验选用30份玉米样品,通过酶联免疫吸附(ELISA)法和高效液相色谱-质谱联用(HPLC-MS)法对玉米中黄曲霉毒素B1含量(AFB1)进行测定比较,得出两种方法的差异性和相关性。其中,使用HPLC-MS外标法定量时,设计两组标准曲线,分别是玉米基质液加标组曲线和纯标组曲线,得到的结果再与ELISA比较。结果显示:用ELISA筛选出的阳性样品,用HPLC-MS法确证亦为阳性,判定结果一致,但具体检出量存在差异。当样品中AFB1含量为2～6μg/kg时,测定结果 HPLC-MS(纯标组) Thirty maize samples were selected in this experiment. AFB1 in maize was detected and compared by ELISA and HPLC-MS to get the differences and correlations between the two methods. The results showed that positive sample screened by ELISA were comfirmed by HPLC-MS,but there were differences in their detectable amount. The result was HPLC-MS(pure mark group) < ELISA < HPLC-MS(maize substrate liquid add mark group) when content aflatoxin B1 in sample was from 2 to 6 μg/kg,and the result of HPLC-MS(maize substrate liquid add mark group)≈ELISA or HPLC-MS(pure mark group) multiplied by 2 to 13. The result was ELISA < HPLC-MS(pure mark group)≈ HPLC-MS(maize substrate liquid add mark group) when content aflatoxin B1 in sample was from 11 to 50 μg/kg. And the result was HPLC-MS(pure mark group)≈HPLC-MS(maize substrate liquid add mark group)+ 3.6 μg/kg whose content aflatoxin B1 in sample lower than61.78 μg/kg. The results suggested that ELISA was a simple and rapid method with high accuracy,and it was suitable for fast screening of aflatoxin B1 in large number of maize samples. While HPLC-MS method takes longer and costs higher to detect,so it was more suitable for the confirmation of positive sample preliminarily screened by ELISA,and the accurate quantitative of aflatoxin B1 content.
引文
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[15]殷秋妙,吴维师,李亚菲,等.液相色谱串联质谱同时测定饲料中6种霉菌毒素技术研究进展[J].中国农学通报,2018,34(14):140~148.
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[2]高秀芬,荫士安,张宏元,等.中国部分地区玉米中4种黄曲霉毒素污染调查[J].卫生研究,2011,40(1):46~49.
[3]何方洋,于信念,王坤,等.酶联免疫法与液相色谱-串联质谱法检测猪肉中沙丁胺醇[J].中国酿造,2015,34(8):132~135.
[4]黄俊恒,黄广明.2017年21省市饲料及饲料原料霉菌毒素污染状况分析[J].养猪,2018,20(3):20~22.
[5]贾卫昌,朱寅.高效液相色谱法与酶联免疫法检测小麦中呕吐毒素的比较研究[J].粮食与食品工业,2015,22(3):97~99.
[6]李少晖,任丹丹,谢云峰,等.食品中黄曲霉毒素检测方法研究进展[J].食品安全质量检测学报,2015,6(4):1111~1115.
[7]刘柱,陈万勤,沈潇冰,等.多功能柱净化-柱后光化学衍生-高效液相色谱法同时检测玉米和花生中9种真菌毒素[J].分析科学学报,2014,30(2):168~172.
[8]柳其芳.酶联免疫吸附法和薄层色谱法联合分析黄曲霉毒素B1的研究[J].中国热带医学,2006,6(2):246~248.
[9]马晓宇,林英庭.饲料中霉菌毒素的检测技术[J].粮食与饲料工业,2018,3:57~61.
[10]王瑞国,苏晓鸥,程芳芳,等.液相色谱串联质谱法测定饲料原料中26种霉菌毒素[J].分析化学研究报告,2015,43(2):264~270.
[11]王晓琳,杜红珍,李增宁,等.毛细管电泳-化学发光联用法检测粮食中的伏马菌素B1[J].中国食品卫生杂志,2015,27(4):382~385.
[12]谢文梅,张兰,苏永腾,等.2017年1~6月饲料及原料霉菌毒素分析报告[J].饲料广角,2017,9:28~31.
[13]徐炎炎,李森,张芹,等.气质联用和液质联用中基质效应的分析和总结[J].农药,2017,56(3):162~167.
[14]肖桦,买娜,王旭峰,等.酶联免疫吸附法在植物性食品安全检测中的应用[J].食品科学,2006,27(12):920~923.
[15]殷秋妙,吴维师,李亚菲,等.液相色谱串联质谱同时测定饲料中6种霉菌毒素技术研究进展[J].中国农学通报,2018,34(14):140~148.
[16]张明,黄解珠,龙俊敏,等.饲料及饲料原料中霉菌毒素检测方法的研究进展[J].江西饲料,2018,1:1~4.
[17] Iarc monographs on the evaluation of carcinogenic risks to humans.Ingested nitrate and nitrite,and cyanobacterial peptide toxins[J].Iarcm onogr Eval Carcinog Risks Hum,2010,94:1~412
[18] Matuszewski B K,Constanzer M L,Chavwz-eng C M.Strategies for the assessment of matrix effect in quantitative bioan-alytical methods based on HPLC-MS/MS[J]. Anal chem,2003,75(13):3019~3030.
[19] Oplatowska-stachowiak M,Sajic N,XU Y,et al.Fast and sensitive aflatoxin B1and total aflatoxins ELISAs for analysis of peanuts,maize and feed ingredients[J].Food Control,2016,63:239~245.