分光光度法测定冻干粉针剂和滴眼液中甲磺酸帕珠沙星
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摘要
在硝酸溶液中,铁(Ⅲ)与甲磺酸帕珠沙星在室温下形成橙黄色配合物,其最大吸收波长为470nm,采用分光光度法测定冻干粉针剂和滴眼液中甲磺酸帕珠沙星的含量。甲磺酸帕珠沙星的质量浓度在3.0～540.0mg·L~(-1)内与其对应的吸光度呈线性关系,检出限(3s/k)为0.88mg·L~(-1)。方法用于冻干粉针剂和滴眼液样品的分析,加标回收率为96.8%～101%,测定值的相对标准偏差(n=5)为1.3%～1.4%。
In HNO_3 solution,the orange-yellow coordination compound of Fe(Ⅲ) and pazufloxacin mesilate was formed at room temperature,having its absorption maximum at the wavelength of 470 nm.Spectrophotometry was applied to the determination of pazufloxacin mesylate in lyophilized powder injections and eye drops.Linear relationship between values of absorbance and mass concentration of pazufloxacin mesilate was obtained in the range of 3.0-540.0 mg·L~(-1),with detection limit(3s/k) of 0.88 mg·L~(-1).The proposed method was applied to the analysis of samples of lyophilized powder injection and eye drop,giving values of recovery found by standard addition method and RSDs(n=5) of determined values in the ranges of 96.8%-101% and 1.3%-1.4% respectively.
In HNO_3 solution,the orange-yellow coordination compound of Fe(Ⅲ) and pazufloxacin mesilate was formed at room temperature,having its absorption maximum at the wavelength of 470 nm.Spectrophotometry was applied to the determination of pazufloxacin mesylate in lyophilized powder injections and eye drops.Linear relationship between values of absorbance and mass concentration of pazufloxacin mesilate was obtained in the range of 3.0-540.0 mg·L~(-1),with detection limit(3s/k) of 0.88 mg·L~(-1).The proposed method was applied to the analysis of samples of lyophilized powder injection and eye drop,giving values of recovery found by standard addition method and RSDs(n=5) of determined values in the ranges of 96.8%-101% and 1.3%-1.4% respectively.
引文
[1]田军军,邱宇,刘艳丽.反相高效液相色谱法测定甲磺酸帕珠沙星原料及其制剂含量[J].哈尔滨医科大学学报,2005,39(4):370-372.
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[9]任耘,袁恒杰,闫涛,等.高效液相色谱法测定血清中甲磺酸帕珠沙星的浓度[J].中国医院药学杂志,2006,26(4):418-420.
[2]杨峰,张迎雪,章竹君.高效毛细管电泳法测定人血及尿样中甲磺酸帕珠沙星[J].分析测试学报,2007,26(2):249-251.
[3]张晶,薛爱芳,糜志远,等.紫外分光光度法测定甲磺酸帕珠沙星原料药含量[J].光谱实验室,2006,23(5):930-932.
[4] PHAPALE P B,LEE H W,KIM S D,et al.Analysis of pazufloxacin mesilate in human plasma and urine by LC with fluorescence and UV detection,and its application to pharmacokinetic study[J].Chromatographia,2010,71(1/2):101-106.
[5] CAO X L,LIAN L L,LI H W,et al.A fluorescence probe based on biomolecule-stabilized gold nanoclusters for the detection of pazufloxacin mesilate[J].Analytical Sciences,2014,30(8):817-822.
[6]李惠颖,王磊,马骏飞,等.铕敏化荧光法测定甲磺酸帕苏沙星[J].分析化学,2006,34(增刊1):207-210.
[7] ZHOU L,YE G R,YUAN R,et al.A capacitive sensor based on molecularly imprinted polymers and poly(p-aminobenzene sulfonic acid)film for detection of pazufloxacin mesilate[J].Science in China Series B:Chemistry,2007,50(4):547-553.
[8] YANG C Y,ZHANG Z J,CHEN S M,et al.Molecularly imprinted on-line solid-phase extraction combined with chemiluminescence for the determination of pazufloxacin mesilate[J].Microchimica Acta,2007,159(3/4):299-304.
[9]任耘,袁恒杰,闫涛,等.高效液相色谱法测定血清中甲磺酸帕珠沙星的浓度[J].中国医院药学杂志,2006,26(4):418-420.