酶法提取枳实中橙皮苷工艺研究
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摘要
目的在碱提酸沉法基础上,探索酶法从枳实中提取橙皮苷的工艺。方法采用单因素试验对加酶量、料水比、酶解时间、酶解温度进行初步筛选,并采用L_9(3~4)正交试验优化酶解提取条件。结果优选得到的最佳酶解条件为:加酶量0.5%,料水比1:5,酶解时间2 h,酶解温度50℃。在此最佳工艺条件下,橙皮苷的收率为25.0%。结论优选的酶法提取工艺有效降低了酸碱用量和废水排放,为生产工艺改进提供参考依据。
Objective To explore the enzyme assisted extraction process of hesperidin from Aurantii fructus immaturus on the basis of alkali extraction and acid precipitation process. Methods The enzyme concentration, the ratio between material and water, enzymolysis time and enzymolysis temperature were screened preliminarily by single factor test. And then, the enzymolysis conditions were optimized by L_9(3~4) orthogonal test. Results The optimum enzymolysis conditions were as follows: the enzyme concentration was 0.5 %, the ratio between material and water was 1:5, the enzymolysis time was 2 h, and the enzymolysis temperature 50 ℃. Under these conditions, the yield of hesperidin reached 25.0 %. Conclusion The optimized enzyme assisted extraction process can reduce the acid and alkali consumption and waste water discharge, thus provide reference for improvement of production process.
Objective To explore the enzyme assisted extraction process of hesperidin from Aurantii fructus immaturus on the basis of alkali extraction and acid precipitation process. Methods The enzyme concentration, the ratio between material and water, enzymolysis time and enzymolysis temperature were screened preliminarily by single factor test. And then, the enzymolysis conditions were optimized by L_9(3~4) orthogonal test. Results The optimum enzymolysis conditions were as follows: the enzyme concentration was 0.5 %, the ratio between material and water was 1:5, the enzymolysis time was 2 h, and the enzymolysis temperature 50 ℃. Under these conditions, the yield of hesperidin reached 25.0 %. Conclusion The optimized enzyme assisted extraction process can reduce the acid and alkali consumption and waste water discharge, thus provide reference for improvement of production process.
引文
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[5]王云洁,闫治攀,白福祖.酶法在中药提取中的应用进展[J].中国中医药信息杂志,2013,20(9):110-112.
[6]曹峰.四种果皮中橙皮苷含量的检测[J].山东农业大学学报(自然科学版),2016,47(3):404-407.
[7]王建安,江海,陈文强,等.柑桔组织中橙皮苷的分析[J].湖北农业科学,2013,52(7):1659-1662.
[8]Kohno H,Taima M,Sumida T,et al.Inhibitory effect of mandarin juice rich in beta-cryptoxanthin and hesperidin on4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced pulmonary tumorigenesis in mice[J].Cancer Lett,2001,174(2):141-150.
[9]Marder M,Viola H,Wasowski C,et al.6-Bethylapigenin and hesperidin:new valeriana flavonoids with activity on the CNS[J].Pharmacol Biochem Behav,2003,75(3):537-545..
[10]Grohmann K,Manthey J A,Cameron R G.Acid-catalyzed hydrolysis of hesperidin at elevated temperatures[J].Carbohydr Res,2000,328(2):141-146.
[2]许玲玲,杨晓东,李群力,等.果胶酶解法提取陈皮中橙皮苷的工艺研究[J].中国现代中药,2015,17(11):1196-1200.
[3]Deng W G,Fang X W,Wu J L.Flavonoids function as antioxidants:By scavenging reactive oxygen species or by chelating iron?[J].Radiat Phys Chem,1997,50(3):271-276.
[4]王淳,刘振丽,宋志前.中药枳疾提取工艺研究[J].中国实验方剂学杂志,2008,14(9):23-25.
[5]王云洁,闫治攀,白福祖.酶法在中药提取中的应用进展[J].中国中医药信息杂志,2013,20(9):110-112.
[6]曹峰.四种果皮中橙皮苷含量的检测[J].山东农业大学学报(自然科学版),2016,47(3):404-407.
[7]王建安,江海,陈文强,等.柑桔组织中橙皮苷的分析[J].湖北农业科学,2013,52(7):1659-1662.
[8]Kohno H,Taima M,Sumida T,et al.Inhibitory effect of mandarin juice rich in beta-cryptoxanthin and hesperidin on4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced pulmonary tumorigenesis in mice[J].Cancer Lett,2001,174(2):141-150.
[9]Marder M,Viola H,Wasowski C,et al.6-Bethylapigenin and hesperidin:new valeriana flavonoids with activity on the CNS[J].Pharmacol Biochem Behav,2003,75(3):537-545..
[10]Grohmann K,Manthey J A,Cameron R G.Acid-catalyzed hydrolysis of hesperidin at elevated temperatures[J].Carbohydr Res,2000,328(2):141-146.