宽体金线蛭5个地理群体遗传多样性的RAPD分析及生长指标的比较研究
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摘要
利用RAPD标记技术对宽体金线蛭5个地理群体的遗传多样性进行RAPD分析,并对其生长指标进行了比较研究。结果表明:江苏兴化和黑龙江宾县群体间的亲缘关系最远,遗传差异性较大,江苏兴化和江苏宿迁群体间的亲缘关系最近,遗传差异性较小;聚类分析显示江苏地区3个群体和湖北大悟县群体聚为第一大类,黑龙江宾县群体聚为第二大类,其中第一大类中江苏兴化和江苏宿迁群体聚为第一亚类,江苏吴江和湖北大悟县群体聚为第二亚类;无论是仔蛭还是幼蛭黑龙江宾县的生长指标都明显低于其他4个地区。
RAPD markers were used to analyze the genetic diversity of five geographical populations of Whitmania pigra,and their growth indexes were compared in this study. The results showed that: the maximum genetic distance appeared between the populations of Xinghua( Jiangsu) and Binxian( Heilongjiang),reflecting larger genetic differences; the minimum genetic distance occurred between the populations of Xinghua and Suqian( Jiangsu),reflecting smaller genetic differences. Cluster analysis indicated that the three populations from Jiangsu and the population of Dawu( Hubei) were divided into the first group,while the population of Binxian was divided into the second group alone. In the first group,the populations of Xinghua and Suqian were divided into the first subgroup,and the populations of Wujiang( Jiangsu) and Dawu were divided into the second subgroup. The growth indexes of young W. pigra from Binxian county of Heilongjiang province were significantly lower than those from other four regions.
RAPD markers were used to analyze the genetic diversity of five geographical populations of Whitmania pigra,and their growth indexes were compared in this study. The results showed that: the maximum genetic distance appeared between the populations of Xinghua( Jiangsu) and Binxian( Heilongjiang),reflecting larger genetic differences; the minimum genetic distance occurred between the populations of Xinghua and Suqian( Jiangsu),reflecting smaller genetic differences. Cluster analysis indicated that the three populations from Jiangsu and the population of Dawu( Hubei) were divided into the first group,while the population of Binxian was divided into the second group alone. In the first group,the populations of Xinghua and Suqian were divided into the first subgroup,and the populations of Wujiang( Jiangsu) and Dawu were divided into the second subgroup. The growth indexes of young W. pigra from Binxian county of Heilongjiang province were significantly lower than those from other four regions.
引文
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[19]杨慧荣,江世贵,周发林,等.3个不同地理群体黑鲷遗传变异的RAPD分析[J].中国水产科学,2004,11(3):185-189.
[20]王宣朋,王宣忠,王信海,等.不同温度、饵料对宽体金线蛭仔蛭生长和存活的影响[J].福建水产,2014,36(3):241-246.
[21]熊良伟,王帅兵,王建国.宽体金线蛭繁殖性能及蛭苗生长特征研究[J].上海海洋大学学报,2016,25(3):374-380.
[22]王安纲,王祖效.宽体金线蛭的调查及生物学特性的观察[J].水利渔业,2005,25(5):40-41.
[2]段超,刘娟.仿生诱导高效提取水蛭素及分离纯化的研究[J].黑龙江医药科学,2008,31(3):40-41.
[3]李东方,李艳春,郑远洋,等.水蛭的生物学特性及养殖技术[J].黑龙江水产,2008(4):6-7.
[4]高明,侯建华,李双安,等.温度对宽体金线蛭繁殖的影响研究[J].安徽农业科学,2012,40(22):11305-11306.
[5]王信海,丁辰龙,姜爱兰,等.宽体金线蛭的人工可控高效繁殖技术[J].水产养殖,2012(2):30-31.
[6]史红专,刘飞,郭巧生.温度和体重对蚂蟥人工繁殖影响的研究[J].中国中药杂志,2006,31(24):2030-2032.
[7]丁辰龙,王宣朋,王信海,等.宽体金线蛭4个繁殖性状指标的相关性分析[J].福建水产,2012,34(6):488-492.
[8]李军,于翔,宋文华,等.宽体金线蛭室内产茧、孵化及仔蛭饥饿研究[J].中国现代中药,2012,14(11):31-36.
[9]张涛,于翔,龚元,等.宽体金线蛭室内繁殖的初步研究[J].水产学杂志,2014,27(2):52-60.
[10]王宣朋,王宣忠,王信海,等.不同温度、饵料对宽体金线蛭仔蛭生长和存活的影响[J].渔业研究,2014,36(3):241-246.
[11]林小清,徐海圣,沈乃峰,等.不同饵料对宽体金线蛭生长的影响[J].浙江农业科学,2014(9):1447-1449.
[12]熊良伟,王帅兵,王建国.宽体金线蛭繁殖性能及蛭苗生长特征研究[J].上海海洋大学学报,2016,25(3):374-380.
[13]吴学军,王信海,丁辰龙,等.水泥池水蛭—莲藕—螺蛳高效生态立体繁、养新技术[J].水产养殖,2014(5):40-42.
[14]丁辰龙,蔺玉华,王信海,等.水蛭养殖池塘生物组成与生长条件的研究[J].天津师范大学学报:自然科学版,2014,33(4):64-67.
[15] Wells S, W Coombes. The status of and trade in the medicinal leech[J]. Traffic Bulletin,1987(8):64-69.
[16]Zhang B,Lin Q,Lin J D,et al. Effects of broodstock density and diet on reproduction and juvenile culture of the Leech,Hirudinaria manillensis Lesson[J]. Journal of Aquaculture,2008,276:198-204.
[17]梁超,杨爱国,刘志鸿,等.魁蚶4个地理群体遗传结构的RAPD分析[J].渔业科学进展,2010,31(1):59-64.
[18]蒋速飞,傅洪拓,熊贻伟,等.日本沼虾4个地理群体遗传变异的RAPD分析[J].长江大学学报:(自科版)农学卷,2006,3(2):179-182.
[19]杨慧荣,江世贵,周发林,等.3个不同地理群体黑鲷遗传变异的RAPD分析[J].中国水产科学,2004,11(3):185-189.
[20]王宣朋,王宣忠,王信海,等.不同温度、饵料对宽体金线蛭仔蛭生长和存活的影响[J].福建水产,2014,36(3):241-246.
[21]熊良伟,王帅兵,王建国.宽体金线蛭繁殖性能及蛭苗生长特征研究[J].上海海洋大学学报,2016,25(3):374-380.
[22]王安纲,王祖效.宽体金线蛭的调查及生物学特性的观察[J].水利渔业,2005,25(5):40-41.