不同类型微生物对低品位磷矿的溶解作用研究
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摘要
通过磷矿样本富集、分离纯化、组合获得了菌群、单一菌株和复合菌株3种类型微生物,研究了3种类型微生物对低品位磷矿的溶解作用。结果表明,3种类型微生物在NBRIP培养基中均能有效溶解低品位磷矿,其对低品位磷矿的溶解作用与培养基pH值降低有关。红外光谱分析结果表明,在不同微生物的作用下矿物表面官能团变化相似,且培养基中可溶磷含量与3400～2500 cm~(-1)处的吸收峰强度有一定相关性。在3种类型微生物中,以菌群的溶磷效果最佳,其次是复合菌株,单一菌株溶磷效果相对较差。
Three types of microorganisms including microbial consortium, single bacterial strain and complex bacterial strains were obtained from phosphate ore samples by enrichment, separation and purification, as well as combination. And the dissolution of low-grade phosphate ore by these three types of microorganisms was studied. Results showed that the low-grade phosphate ore could be effectively dissolved in NBRIP medium by these three microorganisms, and the dissolution effect varied with the decreasing of pH value of medium. The infrared spectroscopy analysis showed the variations of the functional groups on the surface of minerals were similar under the action of different microorganisms, and the content of soluble phosphorus in the medium had something to do with the intensity of the absorption peak at 3400～2500 cm~(-1). It was finally found that among the three different types of microorganisms, microbial consortium could bring in the best phosphate dissolution effect, followed by the complex bacterial strain and the single strain.
Three types of microorganisms including microbial consortium, single bacterial strain and complex bacterial strains were obtained from phosphate ore samples by enrichment, separation and purification, as well as combination. And the dissolution of low-grade phosphate ore by these three types of microorganisms was studied. Results showed that the low-grade phosphate ore could be effectively dissolved in NBRIP medium by these three microorganisms, and the dissolution effect varied with the decreasing of pH value of medium. The infrared spectroscopy analysis showed the variations of the functional groups on the surface of minerals were similar under the action of different microorganisms, and the content of soluble phosphorus in the medium had something to do with the intensity of the absorption peak at 3400～2500 cm~(-1). It was finally found that among the three different types of microorganisms, microbial consortium could bring in the best phosphate dissolution effect, followed by the complex bacterial strain and the single strain.
引文
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[12] 乔志伟,洪坚平,刘超.最佳组合溶磷细菌的筛选及培养条件的优化[J].中国土壤与肥料,2017(5):141-146.
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[14] 李雁峰,冯冲凌,李科林,等.复合功能菌群的构建及其对铅锌胁迫下蓖麻种子萌发和铅锌积累的影响[J].中南林业科技大学学报,2017,37(10):45-53.
[15] Chaparro J M,Badri D V,Bakker M G,et al.Root Exudation of Phytochemicals in Arabidopsis Follows Specific Patterns That Are Developmentally Programmed and Correlate with Soil Microbial Functions[J].Plos One,2013,8(2):525-534.
[2] 余永富,葛英勇,潘昌林.磷矿选矿进展及存在的问题[J].矿冶工程,2008,28(1):29-33.
[3] 刘朋,葛英勇,刘鸣,等.四川某磷矿双反浮选试验研究[J].矿冶工程,2018,38(3):63-65.
[4] 杨峻杰,庹必阳,韩朗,等.低品位磷矿浮选试验研究[J].矿冶工程,2016,36(5):49-52.
[5] 纪斌,孙伟,王若林.十二胺反浮选胶磷矿的消泡机理研究[J].矿冶工程,2018,38(2):47-50.
[6] 许宝科,顾帼华,暨静,等.中温及嗜热菌对不同成因黄铜矿浸出行为的影响[J].矿冶工程,2018,38(1):95-98.
[7] 杨顺,杨婷,林斌,等.两株溶磷真菌的筛选、鉴定及溶磷效果的评价[J].微生物学报,2018(2):264-273.
[8] Maisa M A,Ibrahim E E,Mohamed G E,et al.Effect of mineral constituents in the bioleaching of uranium from uraniferous sedimentary rock samples,Southwestern Sinai,Egypt[J].Journal of Environmental Radioactivity,2014,134:76-82.
[9] Wang E W,Lei S M,Gong W Q.Improved phosphate ore leaching with physical mutagenized Acidithiobacillus ferrooxidans based on modified logistic model[J].Applied Mechanics and Materials,2012,214:520-524.
[10] Xiao C Q,Chi R A,Fang Y J.Effects of Acidiphilium cryptum on biosolubilization of rock phosphate in the presence of Acidithiobacillus ferrooxidans[J].Transactions of Nonferrous Metals Society of China,2013,23(7):2153-2159.
[11] 吴晓燕,肖春桥,池汝安.矿石粒度与矿浆浓度对混合菌溶解低品位磷矿粉的影响[J].矿冶工程,2015,35(4):87-90.
[12] 乔志伟,洪坚平,刘超.最佳组合溶磷细菌的筛选及培养条件的优化[J].中国土壤与肥料,2017(5):141-146.
[13] Nautiyal C S.An efficient microbiological growth medium for screening phosphate solubilizing microorganisms[J].Fems Microbiology Letters,1999,170(1):265-270.
[14] 李雁峰,冯冲凌,李科林,等.复合功能菌群的构建及其对铅锌胁迫下蓖麻种子萌发和铅锌积累的影响[J].中南林业科技大学学报,2017,37(10):45-53.
[15] Chaparro J M,Badri D V,Bakker M G,et al.Root Exudation of Phytochemicals in Arabidopsis Follows Specific Patterns That Are Developmentally Programmed and Correlate with Soil Microbial Functions[J].Plos One,2013,8(2):525-534.