饲料类型对茶尺蠖幼虫肠道细菌多样性及幼虫存活率的影响
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摘要
【目的】茶尺蠖是茶园中的重要害虫。研究茶尺蠖寄主食物-肠道菌群-茶尺蠖生长发育三者之间的关系对于茶尺蠖的防治具有重要的理论指导价值。【方法】分析不添加茶叶因子的纯人工饲料和茶树鲜叶对茶尺蠖幼虫的存活影响;用高通量测序技术分析不同饲料饲喂的茶尺蠖幼虫的肠道菌群异同。【结果】取食人工饲料的幼虫死亡率远远高于取食茶树鲜叶的幼虫;取食人工饲料的幼虫肠道细菌多样性和丰富度高于取食茶树鲜叶的幼虫;茶尺蠖幼虫肠道中存在很多促进宿主生长的细菌。【结论】饲料类型影响茶尺蠖幼虫的存活;饲料类型影响茶尺蠖幼虫肠道菌群结构。
[Objective] Ectropis obliqua Prout(Lepidoptera: Geometridae) is the most devastating insect pest of tea plants in China. Studying on the relationship among feed, gut microbial flora and fitness of Ectropis obliqua is of relatively high theoretical value for the management of E. obliqua. [Methods] We analyzed the effect of artificial diet(without tea ingredients) and tea leaves on survival of Ectropis obliqua larvae. We also analyzed the similarities and differences of gut microbial flora in Ectropis obliqua larvae fed with different feeds based on high-throughput sequencing technology. [Results] The mortality of larvae were significantly different. Larvae fed with artificial diet had high mortality. Larvae fed with artificial diet had higher bacterial diversity and evenness than larvae fed with tea leaves. Many bacteria were found in the gut of Ectropis obliqua larvae. [Conclusion] Feed affects the survival and the gut microbial flora of Ectropis obliqua larvae.
[Objective] Ectropis obliqua Prout(Lepidoptera: Geometridae) is the most devastating insect pest of tea plants in China. Studying on the relationship among feed, gut microbial flora and fitness of Ectropis obliqua is of relatively high theoretical value for the management of E. obliqua. [Methods] We analyzed the effect of artificial diet(without tea ingredients) and tea leaves on survival of Ectropis obliqua larvae. We also analyzed the similarities and differences of gut microbial flora in Ectropis obliqua larvae fed with different feeds based on high-throughput sequencing technology. [Results] The mortality of larvae were significantly different. Larvae fed with artificial diet had high mortality. Larvae fed with artificial diet had higher bacterial diversity and evenness than larvae fed with tea leaves. Many bacteria were found in the gut of Ectropis obliqua larvae. [Conclusion] Feed affects the survival and the gut microbial flora of Ectropis obliqua larvae.
引文
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[10]de Fine Licht HH,Schi?tt M,Rogowska-Wrzesinska A,Nygaard S,Roepstorff P,Boomsma JJ.Laccase detoxification mediates the nutritional alliance between leaf-cutting ants and fungus-garden symbionts.Proceedings of the National Academy of Sciences of the United States of America,2013,110(2):583-587.
[11]Chen YG,Huang MD.Biological control progress of Ectropis obliqua Prout.Natural Enemies of Insects,2001,23(4):181-184.(in Chinese)陈亦根,黄明度.茶尺蠖生物防治进展.环境昆虫学报,2001,23(4):181-184.
[12]Gao XH,Wan XC,Yang YQ,Zheng GY,Shen Q.Studies on the biological habits of Ectropis obliqua prout.Plant Protection,2007,33(3):110-113.(in Chinese)高旭晖,宛晓春,杨云秋,郑高云,沈强.茶尺蠖生物学习性研究.植物保护,2007,33(3):110-113.
[13]Wan XC,Li DX,Zhang ZZ,Xia T,Ling TJ,Chen Q.Research advance on tea biochemistry.Journal of Tea Science,2015,35(1):1-10.(in Chinese)宛晓春,李大祥,张正竹,夏涛,凌铁军,陈琪.茶叶生物化学研究进展.茶叶科学,2015,35(1):1-10.
[14]Zeng FR.Research of insect artificial diet.Chinese Journal of Biological Control,2018,34(2):184-197.(in Chinese)曾凡荣.昆虫人工饲料研究.中国生物防治学报,2018,34(2):184-197.
[15]Yang YQ,Zhang Y,Chen YR,Zhang C,Zhao TY,Long YH.Function and research methods of insect intestinal bacteria.Journal of Anhui Agricultural University,2018,45(3):512-518.(in Chinese)杨云秋,张勇,陈亦然,张灿,赵天宇,龙雁华.昆虫肠道细菌的功能和研究方法.安徽农业大学学报,2018,45(3):512-518.
[16]Chen BS,Teh BS,Sun C,Hu SR,Lu XM,Boland W,Shao YQ.Biodiversity and activity of the gut microbiota across the life history of the insect herbivore Spodoptera littoralis.Scientific Reports,2016,6:29505.
[17]Guan CH,Ju JH,Borlee BR,Williamson LL,Shen B,Raffa KF,Handelsman J.Signal mimics derived from a metagenomic analysis of the gypsy moth gut microbiota.Applied and Environmental Microbiology,2007,73(11):3669-3676.
[18]Mead LJ,Khachatourians GG,Jones GA.Microbial ecology of the gut in laboratory stocks of the migratory grasshopper,Melanoplus sanguinipes(Fab.)(Orthoptera:Acrididae).Applied and Environmental Microbiology,1988,54(5):1174-1181.
[19]Broderick NA,Raffa KF,Goodman RM,Handelsman J.Census of the bacterial community of the gypsy moth larval midgut by using culturing and culture-independent methods.Applied and Environmental Microbiology,2004,70(1):293-300.
[20]Johnston PR,Crickmore N.Gut bacteria are not required for the insecticidal activity of Bacillus thuringiensis toward the tobacco hornworm,Manduca sexta.Applied and Environmental Microbiology,2009,75(15):5094-5099.
[21]Vilanova C,Baixeras J,Latorre A,Porcar M.The generalist inside the specialist:gut bacterial communities of two insect species feeding on toxic plants are dominated by Enterococcus sp..Frontiers in Microbiology,2016,7:1005.
[22]Mazzafera P.Degradation of caffeine by microorganisms and potential use of decaffeinated coffee husk and pulp in animal feeding.Scientia Agricola,2002,59(4):815-821.
[23]Nathanson JA.Caffeine and related methylxanthines:possible naturally occurring pesticides.Science,1984,226(4671):184-187.
[24]Wang HJ,Dai YK,Pan Q.Research status of Weissella.Journal of Chengdu Medical College,2014,9(6):747-750.(in Chinese)王海娟,戴雨珂,潘渠.魏斯氏菌的研究现状.成都医学院学报,2014,9(6):747-750.
[25]Sun YQ,Guo YH.Determination of different types of Legumes cellulase content.Journal of Liaoning University of Traditional Chinese Medicine,2014,16(10):63-65.(in Chinese)孙艺琦,郭晏华.不同种类豆科植物中纤维素酶含量测定.辽宁中医药大学学报,2014,16(10):63-65.
[26]Singh BN,Shankar S,Srivastava RK.Green tea catechin,epigallocatechin-3-gallate(EGCG):mechanisms,perspectives and clinical applications.Biochemical Pharmacology,2011,82(12):1807-1821.
[27]Bhakdi S,Tranum-Jensen J.Alpha-toxin of Staphylococcus aureus.Microbiological Reviews,1991,55(4):733-751.
[28]Yang HJ,Tan ZJ,Xiao QM,He KJ.Biocontrol functions of Pseudomonas.Chinese Journal of Eco-Agriculture,2004,12(3):158-161.(in Chinese)杨海君,谭周进,肖启明,何可佳.假单胞菌的生物防治作用研究.中国生态农业学报,2004,12(3):158-161.
[2]Chapman RF.The insects:structure and functionnn.London:Cambridge University Press,1998:132-141.
[3]Engel P,Moran NA.The gut microbiota of insects-diversity in structure and function.FEMS Microbiology Reviews,2013,37(5):699-735.
[4]Brune A,Egert M,Lemke T,Friedrich MW,Wagner B.Microbial community structure in midgut and hindgut of the humus-feeding larva of Pachnoda ephippiata(Coleoptera:Scarabaeidae).Applied and Environmental Microbiology,2003,69(11):6659-6668.
[5]Xiang YQ,Wang XQ,Feng W,Zhou W,Xie HX,Wan YJ.Comparative analysis of the composition of dominant intestinal microflora in silkworm reared with different forages.Acta Ecologica Sinica,2010,30(14):3875-3882.(in Chinese)向芸庆,王晓强,冯伟,周围,谢洪霞,万永继.不同饲料饲养家蚕其肠道微生态优势菌群类型的组成及差异性.生态学报,2010,30(14):3875-3882.
[6]Xiang H,Li MW,Zhao Y,Zhao LP,Zhang YH,Huang YP.Bacterial community in midguts of the silkworm larvae estimated by PCR/DGGE and 16S r DNA gene library analysis.Acta Entomologica Sinica,2007,50(3):222-233.(in Chinese)相辉,李木旺,赵勇,赵立平,张月华,黄勇平.家蚕幼虫中肠细菌群落多样性的PCR-DGGE和16S r DNA文库序列分析.昆虫学报,2007,50(3):222-233.
[7]Rosenthal GA,Berenbaum MR.Herbivores:their interactions with secondary plant metabolites Vol II:ecological and evolutionary processes.2nd ed.New York:Academic Press,1992.
[8]Itoh H,Tago K,Hayatsu M,Kikuchi Y.Detoxifying symbiosis:microbe-mediated detoxification of phytotoxins and pesticides in insects.Natural Product Reports,2018,35(5):434-454.
[9]Ceja-Navarro JA,Vega FE,Karaoz U,Hao Z,Jenkins S,Lim HC,Kosina P,Infant F,Northen TR,Brodie EL.Gut microbiota mediate caffeine detoxification in the primary insect pest of coffee.Nature Communications,2015,6:7618.
[10]de Fine Licht HH,Schi?tt M,Rogowska-Wrzesinska A,Nygaard S,Roepstorff P,Boomsma JJ.Laccase detoxification mediates the nutritional alliance between leaf-cutting ants and fungus-garden symbionts.Proceedings of the National Academy of Sciences of the United States of America,2013,110(2):583-587.
[11]Chen YG,Huang MD.Biological control progress of Ectropis obliqua Prout.Natural Enemies of Insects,2001,23(4):181-184.(in Chinese)陈亦根,黄明度.茶尺蠖生物防治进展.环境昆虫学报,2001,23(4):181-184.
[12]Gao XH,Wan XC,Yang YQ,Zheng GY,Shen Q.Studies on the biological habits of Ectropis obliqua prout.Plant Protection,2007,33(3):110-113.(in Chinese)高旭晖,宛晓春,杨云秋,郑高云,沈强.茶尺蠖生物学习性研究.植物保护,2007,33(3):110-113.
[13]Wan XC,Li DX,Zhang ZZ,Xia T,Ling TJ,Chen Q.Research advance on tea biochemistry.Journal of Tea Science,2015,35(1):1-10.(in Chinese)宛晓春,李大祥,张正竹,夏涛,凌铁军,陈琪.茶叶生物化学研究进展.茶叶科学,2015,35(1):1-10.
[14]Zeng FR.Research of insect artificial diet.Chinese Journal of Biological Control,2018,34(2):184-197.(in Chinese)曾凡荣.昆虫人工饲料研究.中国生物防治学报,2018,34(2):184-197.
[15]Yang YQ,Zhang Y,Chen YR,Zhang C,Zhao TY,Long YH.Function and research methods of insect intestinal bacteria.Journal of Anhui Agricultural University,2018,45(3):512-518.(in Chinese)杨云秋,张勇,陈亦然,张灿,赵天宇,龙雁华.昆虫肠道细菌的功能和研究方法.安徽农业大学学报,2018,45(3):512-518.
[16]Chen BS,Teh BS,Sun C,Hu SR,Lu XM,Boland W,Shao YQ.Biodiversity and activity of the gut microbiota across the life history of the insect herbivore Spodoptera littoralis.Scientific Reports,2016,6:29505.
[17]Guan CH,Ju JH,Borlee BR,Williamson LL,Shen B,Raffa KF,Handelsman J.Signal mimics derived from a metagenomic analysis of the gypsy moth gut microbiota.Applied and Environmental Microbiology,2007,73(11):3669-3676.
[18]Mead LJ,Khachatourians GG,Jones GA.Microbial ecology of the gut in laboratory stocks of the migratory grasshopper,Melanoplus sanguinipes(Fab.)(Orthoptera:Acrididae).Applied and Environmental Microbiology,1988,54(5):1174-1181.
[19]Broderick NA,Raffa KF,Goodman RM,Handelsman J.Census of the bacterial community of the gypsy moth larval midgut by using culturing and culture-independent methods.Applied and Environmental Microbiology,2004,70(1):293-300.
[20]Johnston PR,Crickmore N.Gut bacteria are not required for the insecticidal activity of Bacillus thuringiensis toward the tobacco hornworm,Manduca sexta.Applied and Environmental Microbiology,2009,75(15):5094-5099.
[21]Vilanova C,Baixeras J,Latorre A,Porcar M.The generalist inside the specialist:gut bacterial communities of two insect species feeding on toxic plants are dominated by Enterococcus sp..Frontiers in Microbiology,2016,7:1005.
[22]Mazzafera P.Degradation of caffeine by microorganisms and potential use of decaffeinated coffee husk and pulp in animal feeding.Scientia Agricola,2002,59(4):815-821.
[23]Nathanson JA.Caffeine and related methylxanthines:possible naturally occurring pesticides.Science,1984,226(4671):184-187.
[24]Wang HJ,Dai YK,Pan Q.Research status of Weissella.Journal of Chengdu Medical College,2014,9(6):747-750.(in Chinese)王海娟,戴雨珂,潘渠.魏斯氏菌的研究现状.成都医学院学报,2014,9(6):747-750.
[25]Sun YQ,Guo YH.Determination of different types of Legumes cellulase content.Journal of Liaoning University of Traditional Chinese Medicine,2014,16(10):63-65.(in Chinese)孙艺琦,郭晏华.不同种类豆科植物中纤维素酶含量测定.辽宁中医药大学学报,2014,16(10):63-65.
[26]Singh BN,Shankar S,Srivastava RK.Green tea catechin,epigallocatechin-3-gallate(EGCG):mechanisms,perspectives and clinical applications.Biochemical Pharmacology,2011,82(12):1807-1821.
[27]Bhakdi S,Tranum-Jensen J.Alpha-toxin of Staphylococcus aureus.Microbiological Reviews,1991,55(4):733-751.
[28]Yang HJ,Tan ZJ,Xiao QM,He KJ.Biocontrol functions of Pseudomonas.Chinese Journal of Eco-Agriculture,2004,12(3):158-161.(in Chinese)杨海君,谭周进,肖启明,何可佳.假单胞菌的生物防治作用研究.中国生态农业学报,2004,12(3):158-161.