基于1-乙烯基-3-甲基咪唑的牛血清白蛋白分子印迹研究
|
摘要
采用分子印迹技术,以氧化石墨烯为基质,牛血清白蛋白(Bovine serum albumin,BSA)为模板分子,1-乙烯基-3-甲基咪唑与丙烯酰胺为功能单体,N,N`-亚甲基双丙酰胺为交联剂,制备出分子印迹聚合物,并优化了该印记聚合物的合成条件和吸附条件;并对其动力学吸附性能、等温吸附性能等进行了评价。
By using molecular imprinting technique,graphene oxide as matrix,bovine serum albumin(BSA)as template molecule,1-ehenyl-3-Methylimidazolium iodide and acrylamide as the functional monomer,N,N'-methylenebisacrylamide as crosslinking agent,preparation of molecularly imprinted polymers and optimize the conditions for the synthesis of molecularly imprinted polymer and adsorption conditions. The dynamic adsorption properties,and isothermal adsorption properties were characterized.
By using molecular imprinting technique,graphene oxide as matrix,bovine serum albumin(BSA)as template molecule,1-ehenyl-3-Methylimidazolium iodide and acrylamide as the functional monomer,N,N'-methylenebisacrylamide as crosslinking agent,preparation of molecularly imprinted polymers and optimize the conditions for the synthesis of molecularly imprinted polymer and adsorption conditions. The dynamic adsorption properties,and isothermal adsorption properties were characterized.
引文
[1] DF Tai,CY Lin. Recognition of dengue virus protein using epitope-mediated molecularly imprinted film[J]. Anal Chem. 2005,77(16):5140–5143.
[2] G Wulff. Enzyme-like catalysis by molecularly imprinted polymers[J]. Chemical Reviews,2002,102(1):1-27.
[3]韩霜.分子印迹技术及其研究进展[D].东北石油大学,2014.
[4]肖忠华.烷基咪唑类离子液体在分子印迹聚合物中的应用[J].中国现代应用药学,2016,33(4):513-516.
[5]王慧,陈斌,周元臻,等.离子液体在蛋白质电化学中的应用研究[J].应用化工,2011,40(2):324-326.
[6]冯小黎,金业涛,苏志国.分离纯化中蛋白质的不稳定性及其对策[J].生物工程进展. 2000,20(3):67-71.
[7] DH Cheng,XW Chen,Y Shu,et al. Extraction and separation of the heme proteins from biological samples matrixes by applying ionic liquid(C4tmsimPF6)[J]. Talanta,2008,75(5):1270-1278.
[8]陈旭伟,毛全兴,王建华,等.离子液体在蛋白质萃取分离中的应用[J].化学进展,2013,25(5):662-667.
[9]陈荣枫.以离子液体为功能单体的碳纳米管表面牛血清白蛋白分子印迹聚合物的制备及其性能研究[D].华中农业大学,2014.
[2] G Wulff. Enzyme-like catalysis by molecularly imprinted polymers[J]. Chemical Reviews,2002,102(1):1-27.
[3]韩霜.分子印迹技术及其研究进展[D].东北石油大学,2014.
[4]肖忠华.烷基咪唑类离子液体在分子印迹聚合物中的应用[J].中国现代应用药学,2016,33(4):513-516.
[5]王慧,陈斌,周元臻,等.离子液体在蛋白质电化学中的应用研究[J].应用化工,2011,40(2):324-326.
[6]冯小黎,金业涛,苏志国.分离纯化中蛋白质的不稳定性及其对策[J].生物工程进展. 2000,20(3):67-71.
[7] DH Cheng,XW Chen,Y Shu,et al. Extraction and separation of the heme proteins from biological samples matrixes by applying ionic liquid(C4tmsimPF6)[J]. Talanta,2008,75(5):1270-1278.
[8]陈旭伟,毛全兴,王建华,等.离子液体在蛋白质萃取分离中的应用[J].化学进展,2013,25(5):662-667.
[9]陈荣枫.以离子液体为功能单体的碳纳米管表面牛血清白蛋白分子印迹聚合物的制备及其性能研究[D].华中农业大学,2014.