牙周组织工程研究进展
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摘要
牙周炎是炎症破坏性疾病,会导致牙周韧带、牙骨质、牙槽骨等牙齿支持组织破坏吸收。牙周炎也是成人失牙最主要的原因。在控制感染的基础上重建牙周组织是牙周病治疗的最终目标。牙周组织重建过程涉及多种软、硬组织(牙周韧带、牙龈、牙骨质和骨),其关键是牙骨质、牙槽骨及牙周韧带锚定结构的重构。该文对牙周组织工程的支架材料、支架的三维结构、支架的抗菌性、牙骨质再生的研究进展予以综述。虽然近年来牙周组织工程领域取得了较大的进展,但迄今为止,这些研究尚处于初级阶段,牙骨质及其与牙周膜界面的修复再生还未能实现。牙周组织再生治疗的有效技术尚需大量动物实验、临床实验验证效果。
Periodontitis is an inflammatory disease that causes loss of the tooth-supporting apparatus,including periodontal ligament,cementum,and alveolar bone. Periodontitis is a major cause of tooth loss for adults. Periodontia reconstruction based on infection control is the key solution to periodontal treatment. For a successful clinical outcome,periodontal regeneration requires the coordinated response of multiple soft and hard tissues( periodontal ligament,gingiva,cementum,and bone) during the wound-healing process. A critical requirement is the ability to promote the formation of functional periodontal attachment between regenerated alveolar bone,and newly formed cementum on the root surface. This review outlines the current advances in scaffold of periodontal tissue engineering,as well as its 3D structure,antibiosis and cementum regeneration. Progress on the development periodontal tissue engineering is in the early stages of development,the regeneration of cementum and periodontal ligament complex is still under research all over the world. The constructs need to be tested in large animal models and,ultimately,human clinical trials.
Periodontitis is an inflammatory disease that causes loss of the tooth-supporting apparatus,including periodontal ligament,cementum,and alveolar bone. Periodontitis is a major cause of tooth loss for adults. Periodontia reconstruction based on infection control is the key solution to periodontal treatment. For a successful clinical outcome,periodontal regeneration requires the coordinated response of multiple soft and hard tissues( periodontal ligament,gingiva,cementum,and bone) during the wound-healing process. A critical requirement is the ability to promote the formation of functional periodontal attachment between regenerated alveolar bone,and newly formed cementum on the root surface. This review outlines the current advances in scaffold of periodontal tissue engineering,as well as its 3D structure,antibiosis and cementum regeneration. Progress on the development periodontal tissue engineering is in the early stages of development,the regeneration of cementum and periodontal ligament complex is still under research all over the world. The constructs need to be tested in large animal models and,ultimately,human clinical trials.
引文
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[3]Chen FM,Zhang J,Zhang M,et al.A review on endogenous regenerative technology in periodontal regenerative medicine[J].Biomaterials.2010;31(31):7892-927.
[4]Gungormus M,Oren EE,Horst JA,et al.Cementomimetics-constructing a cementum-like biomineralizedmicrolayer via amelogenin-derived peptides[J].Inter J Oral Sci,2012,4(2):69-77.
[5]Izumi Y,Aoki A,Yamada Y,et al.Current and future periodontal tissue engineering[J].Periodontol 2000.2011;56(1):166-87.
[6]Guilak F,Butler D L,Goldstein S A,et al.Biomechanics and mechanobiology in functional tissue engineering[J].J Biomech,2014,47(9):1933-1940.
[7]Iwata T,Yamato M,Ishikawa I,et al.Tissue engineering in periodontal tissue[J].Anat Rec(Hoboken).2014;297(1):16-25.
[8]Magan A,Ripamonti U.Biological aspects of periodontal tissue regeneration:cementogenesis and the induction of Sharpey'sfibres[J].SADJ:journal of the South African Dental Association=tydskrif van die Suid-Afrikaanse Tandheelkundige Vereniging,08/2013,68(7):304-306,30 8-312,14 passim.
[9]Han J,Menicanin D,Gronthos S,et al.Stem cells,tissue engineering and periodontal regeneration[J].Aust Dent J,2014,59 Suppl 1:117-130.
[10]Ivanovski S,Vaquette C,Gronthos S,et al.Multiphasic scaffolds for periodontal tissue engineering[J].J Dent Res,2014,93(12):1212-1221.
[11]Annalia A,Luciana G.Natural and synthetic biodegradable polymers:different scaffolds for cell expansion and tissue formation[J].International journal of artificial organs,2014,37(3):187-205.
[12]Yamada S,Yamamoto K,Ikeda T,et al.Potency of fish collagen as a scaffold for regenerative medicine[J].Biomed Res Int,2014,2014(1):302932.
[13]WALTERS B D,STEGEMANN J P.Strategies for directing the structure and function of three-dimensional collagen biomaterials across length scales[J].Actabiomaterialia,2014,10(4):1488-1501.
[14]Ivanov AN,Norkin IA,Puchin'Ian DM.The possibilities and perspectives of using scaffold technology for bone regeneration[J].Tsitologiia,2014,56(8):543-548.
[15]Ferreira AM,Gentile P,Chiono V,et al.Collagen for bone tissue regeneration[J].Actabiomaterialia,2012,8(9):3191-3200.
[16]Jiang H,Zuo Y,Zou Q,et al.Biomimetic spiral-cylindrical scaffold based on hybrid chitosan/cellulose/nano-hydroxyapatite membrane for bone regeneration[J].ACS applied materials&interfaces,2013,5(22):12036-12044.
[17]Mohammadi R,Amini K.Guided bone regeneration of mandibles using chitosan scaffold seeded with characterized uncultured omental adipose-derived stromal vascular fraction:an animal study[J].The International journal of oral&maxillofacial implants,2015,30(1):216-222.
[18]Liu H,Peng H,Wu Y,et al.The promotion of bone regeneration by nanofibrous hydroxyapatite/chitosan scaffolds by effects on integrin-BMP/Smad signaling pathway in BMSCs[J].Biomaterials,2013,34(18):4404-4417.
[19]Park H,Choi B,Nguyen J,et al.Anionic carbohydrate-containing chitosan scaffolds for bone regeneration[J].Carbohydrate polymers,2013,97(2):587-596.
[20]Drewnowska O,Turek B,Carstanjen B,et al.Chitosan--a promising biomaterial in veterinary medicine[J].Polish journal of veterinary sciences,2013,16(4):843-848.
[21]Kim BS,Sung HM,You HK,et al.Effects of fibrinogen concentration on fibrin glue and bone powder scaffolds in bone regeneration[J].Journal of bioscience and bioengineering,2014,118(4):469-475.
[22]Gao X,Usas A,Tang Y,et al.A comparison of bone regeneration with human mesenchymal stem cells and muscle-derived stem cells and the critical role of BMP[J].Biomaterials,2014,35(25):6859-6870.
[23]Li Q,Reed DA,Min L,et al.Lyophilized platelet-rich fibrin(PRF)promotes craniofacial bone regeneration through Runx2[J].International journal of molecular sciences,2014,15(5):8509-8525.
[24]Li Q,Pan S,Dangaria SJ,et al.Platelet-rich fibrin promotes periodontal regeneration and enhances alveolar bone augmentation[J].Biomed Res Int,2013,2013:638043.
[25]He X,Dziak R,Mao K,et al.Integration of a novel injectable nano calcium sulfate/alginate scaffold and BMP2 gene-modified mesenchymal stem cells for bone regeneration[J].Tissue engineering Part A,2013,19(3-4):508-518.
[26]Moshaverinia A,Chen C,Xu X,et al.Bone regeneration potential of stem cells derived from periodontal ligament or gingival tissue sources encapsulated in RGD-modified alginate scaffold[J].Tissue engineering Part A,2014,20(3-4):611-621.
[27]Chae T,Yang H,Leung V,et al.Novel biomimetic hydroxyapatite/alginate nanocomposite fibrous scaffolds for bone tissue regeneration[J].Journal of materials science Materials in medicine,2013,24(8):1885-1894.
[28]Sajesh KM,Jayakumar R,Nair SV,et al.Biocompatible conducting chitosan/polypyrrole-alginate composite scaffold for bone tissue engineering[J].International journal of biological macromolecules,2013,62(3):465-471.
[29]Jang CH,Kim MS,Cho YB,et al.Mastoid obliteration using 3D PCL scaffold in combination with alginate and rh BMP-2[J].International journal of biological macromolecules,2013,62(11):614-622.
[30]Zhao J,Li M.[Application of collagen composite scaffold in vascular tissue engineering〗[J].Zhongguoxiufuchongjianwaikezazhi,2011,25(7):859-862.
[31]Kim BS,Baez CE,Atala A.Biomaterials for tissue engineering[J].World journal of urology,2000,18(1):2-9.
[32]Rahmanian-Schwarz A,Held M,Knoeller T,et al.In vivo biocompatibility and biodegradation of a novel thin and mechanically stable collagen scaffold[J].Journal of biomedical materials research Part A,2014,102(4):1173-1179.
[33]Mcmahon RE,Wang L,Skoracki R,et al.Development of nanomaterials for bone repair and regeneration[J].Journal of biomedical materials research Part B,Applied biomaterials,2013,101(2):387-397.
[34]Li J,Baker B A,Mou X,et al.Biopolymer/Calcium phosphate scaffolds for bone tissue engineering[J].Advanced healthcare materials,2014,3(4):469-484.
[35]Billstrm GH,Blom AW,Larsson S,et al.Application of scaffolds for bone regeneration strategies:current trends and future directions[J].Injury,2013,44(Suppl 1):S28-33.
[36]Park SH,Park DS,Shin JW,et al.Scaffolds for bone tissue engineering fabricated from two different materials by the rapid prototyping technique:PCL versus PLGA[J].J Mater Sci.Mater Med,2012;23(11):2671-2678.
[37]Lv Q,Deng M,Ulery BD,et al.Nano-ceramic composite scaffolds for bioreactor-based bone engineering[J].Clin Orthop Relat Res,2013,471(8):2422-2433.
[38]Lupu-Haber Y,Pinkas O,Boehm S,et al.Functionalized PLGA-doped zirconium oxide ceramics for bone tissue regeneration[J].Biomedical microdevices,2013,15(6):1055-1066.
[39]Seol YJ,Park DY,Park JY,et al.A new method of fabricating robust freeform 3D ceramic scaffolds for bone tissue regeneration[J].Biotechnology and bioengineering,2013,110(5):1444-1455.
[40]Bagno A,Di Bello C.Surface treatments and roughness properties of Ti-based biomaterials[J].J Mater Sci.Mater Med,2004,15(9):935-949.
[41]Bose S,Tarafder S.Calcium phosphate ceramic systems in growth factor and drug delivery for bone tissue engineering:a review[J].Actabiomaterialia,2012,8(4):1401-1421.
[42]Venugopal J,Prabhakaran MP,Zhang Y,et al.Biomimetic hydroxyapatite-containing composite nanofibrous substrates for bone tissue engineering[J].Philosophical transactions,2010,368(1917):2065-2081.
[43]Zhou H,Lee J.Nanoscale hydroxyapatite particles for bone tissue engineering[J].Actabiomaterialia,2011,7(7):2769-2781.
[44]Kikuchi M.Hydroxyapatite/collagen bone-like nanocomposite[J].Biological&pharmaceutical bulletin,2013,36(11):1666-1669.
[45]Wu XN,Miao LY,Yao YF,et al.Electrospun fibrous scaffolds combined with nanoscale hydroxyapatite induce osteogenic differentiation of human periodontal ligament cells[J].Int J Nanomedicine.2014,9:4135-4143.
[46]Langer R,Vacanti JP.Tissue Engineering[J].Science,1993,260(5110):920-926.
[47]Mikos AG,Pham QP,Sharma U.Electrospun Poly(E-caprolactone)Microfiber and Multilayer Nanofiber/Microfiber Scaffolds:Characterization of Scaffolds and Measurement of Cellular Infiltration[J].Biomacromolecules,2006,7(10),2796-2805.
[48]Park CH,Rios HF,Jin Q,et al.Biomimetic hybrid scaffolds for engineering human tooth-ligament interfaces[J].Biomaterials,2010,31(32):5945-5959.
[49]Vaquette C,Fan W,Xiao Y,et al.A biphasic scaffold design combined with cell sheet technology for simultaneous regeneration of alveolar bone/p eriodontal ligament complex[J].Biomaterials,2012,33(22):5560-5573.
[50]Park CH,Rios HF,Jin Q,et al.Tissue engineering bone-ligament complexes using fiber-guiding scaffolds[J].Biomaterials,2012,33(1):137-145.
[51]Huang Y,Duan Y,Ding Y,et al.Versatile,kinetically controlled,high precision electrohydrodynamic writing of micro/nanofibers[J].Scientific Reports,2014,4:5949.
[52]Izumi Y,Aoki A,Yamada Y,et al.Current and future periodontal tissue engineering[J].Periodontology 2000,2011,56:166-187.
[53]Alvarez-Pérez MA,Narayanan S,Zeichner-David M,et al.Molecular cloning,expression and immunolocalization of a novel human cementum-derived protein(CP-23)[J].Bone,2006,38(3):409-419.
[54]Carmona-Rodriguez B,Alvarez-Perez MA,Narayanan S,et al.Human Cementum Protein 1 induces expression of bone and cementum proteins by human gingival fibroblasts[J].Biochem Biophy Res Comm,2007,358(3):763-769.
[55]Villarreal-Ramírez E,Moreno A,Mas-Oliva J,et al.Characterization of recombinant human cementum protein 1(rh CEMP1):Primary role in biomineralization[J].Biochem Biophy Res Comm,2009,384(1):49-54.
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