地榆提取物的体外抑菌活性及机制
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摘要
用乙醇、乙酸乙酯、二氯甲烷、正丁醇等有机溶剂和水分别提取中草药地榆的有效成分,得到有机酸类、三萜类和黄酮类化合物,并分析各提取物对伤寒杆菌和痢疾杆菌的抗菌效果和机理.结果表明:地榆的乙酸乙酯相、乙醇相和正丁醇相提取物对伤寒杆菌和痢疾杆菌有抑菌作用,与对照组相比差异极显著(p<0.01),各提取物对实验菌的最小抑菌质量浓度(MIC)差异较大;地榆提取物中的有机酸类、三萜类和黄酮类化合物有不同程度的抑菌作用;地榆提取物可通过干扰细菌细胞壁合成及抑制细胞膜的形成而抑制伤寒杆菌和痢疾杆菌生长,其抑制作用随提取液质量浓度的增加及作用时间的延长而增强.
The active constituents of Sanguisorba officinalis L.were extracted by ethyl alcohol,ethyl acetate,dichloromethane,n-butanol and water respectively,and the organic acids,triterpenoids and flavonoids were obtained.The antibacterial effects and mechanism of the extracts on Salmonella typhoon and Shigella dysenteric were studied.The results show that there were obvious effects of ethyl acetate,ethyl alcohol and n-butanol of S.officinalis on S.typhoon and S.dysenteric.Compared with the control group,the difference is very significant(p <0.01).The minimum inhibitory concentration(MIC)of different bacterias were obviously different.The organic acids,triterpenoids and flavonoids in the extracts of So.fficinalis have various degree of bacteriostasis.The extracts of So.fficinalis can inhibit growth of St.yphoon and S.dysenteric by obstructing cytoderm synthesis and cell membrane formation.The inhibition increases with the increase of the mass concentration of extract and the prolongation of the action time.
The active constituents of Sanguisorba officinalis L.were extracted by ethyl alcohol,ethyl acetate,dichloromethane,n-butanol and water respectively,and the organic acids,triterpenoids and flavonoids were obtained.The antibacterial effects and mechanism of the extracts on Salmonella typhoon and Shigella dysenteric were studied.The results show that there were obvious effects of ethyl acetate,ethyl alcohol and n-butanol of S.officinalis on S.typhoon and S.dysenteric.Compared with the control group,the difference is very significant(p <0.01).The minimum inhibitory concentration(MIC)of different bacterias were obviously different.The organic acids,triterpenoids and flavonoids in the extracts of So.fficinalis have various degree of bacteriostasis.The extracts of So.fficinalis can inhibit growth of St.yphoon and S.dysenteric by obstructing cytoderm synthesis and cell membrane formation.The inhibition increases with the increase of the mass concentration of extract and the prolongation of the action time.
引文
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[14]张佳,王莹,张峰,等.滤纸片法测定黄花蒿提取物对霉菌的抑制活性[J].湖北农业科学,2009,48(5):1153-1154.(ZHANG Jia,WANG Ying,ZHANG Feng,et al.Mensurating the Antiseptic Activity of Extracts from Artemisia annua L.to Mold with the Filter Paper Method[J].Hubei Agricultural Sciences,2009,48(5):1153-1154.)
[15]孙延忠,曾洪梅,石义萍,等.武夷菌素对番茄灰霉菌(Bocrycis cinerea)的作用方式[J].植物病理学报,2003,33(5):434-438.(SUN Yanzhong,ZENG Hongmei,SHI Yiping,et al.Mode of Action of Wuyiencin on Bocrycis cinerea[J].Acta Phytopathologica Sinica,2003,33(5):434-438.)
[16]许维国,刘洋,刘多见,等.没食子酸抑菌活性分析[J].中国公共卫生,2012,28(10):1329-1331.(XU Weiguo,LIU Yang,LIU Duojian,et al.Antimicrobial Activity of Galic Acid[J].Chinese Journal of Public Health,2012,28(10):1329-1331.)
[17]倪建新,陈木水,林跃虹.远志属植物三萜皂苷及其生物活性研究进展[J].中药材,2011,34(2):317-319.(NIJianxin,CHEN Mushui,LIN Yuehong.Research Progress on Triterpenoid Saponins and Their Biological Activities in Polygalal[J].Journal of Chinese Medicinal Materials,2011,34(2):317-319.)
[18]胡焱,何淞,王瑶.齐墩果酸的抗菌作用研究进展[J].中国新药杂志,2017,26(3):304-308.(HU Yan,HE Song,WANG Yao.Progress in Researches of the Antibacterial Effect of Oleanolic Acid[J].Chinese Journal of New Drugs,2017,26(3):304-308.)
[19]LI Shiyou,ZHANG Zhizhen,CAIN A,et al.Antifungal Activity of Camptothecin,Trifolin,and Hyperoside Isolated fromCamptotheca acuminata[J].Journal of Agricultural and Food Chemistry,2005,53(1):32-37.
[20]LI Zilin,LIU Jincheng,HU Jing,et al.Protective Effects of Hyperoside against Human Umbilical Vein Endothelial Cell Damageinduced by Hydrogen Peroxide[J].Journal of Ethnopharmacology,2012,139(2):388-394.
[21]JURIKOVA T,ROP O,MLCEK J,et al.Phenolic Profile of Edible Honeysuckle Berries(Genus lonicera)and Their Biological Effects[J].Molecules,2011,17(1):61-79.
[22]张军,田子罡,王建华,等.有机酸抑菌分子机理研究进展[J].畜牧兽医学报,2011,42(3):323-328.(ZHANGJun,TIAN Zigang,WANG Jianhua,et al.Advances in Antimicrobial Molecular Mechanism of Organic Acid[J].Acta Veterinaria et Zootechnica Sinica,2011,42(3):323-328.)
[23]沈思,李孚杰,梅光明,等.茯苓皮三萜类物质含量的测定及其抑菌活性的研究[J].食品科学,2009,30(1):95-98.(SHEN Si,LI Fujie,MEI Guangming,et al.Sutdy on Content Determination and Antibacterial Effects of Triterpenes from Sclerotia of Poria cocos[J].Food Science,2009,30(1):95-98.)
[24]游庭活,刘凡,温露,等.黄酮类化合物抑菌作用研究进展[J].中国中药杂志,2013,38(21):3645-3650.(YOUTinghuo,LIU Fan,WEN Lu,et al.Research Progress on Antimicrobial Activities of Flavonoids[J].China Journal of Chinese Materia Medica,2013,38(21):3645-3650.)
[2]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015:126.(Chinese Pharmacopoeia Commission.People’s Republic of China Pharmacopoeia[M].Beijing:China Medical Science and Technology Press,2015:126.)
[3]代良敏,熊永爱,范奎,等.地榆化学成分与药理作用研究进展[J].中国实验方剂学杂志,2016,22(20):189-195.(DAI Liangmin,XIONG Yong’ai,FAN Kui,et al.Research Progress on Chemical Composition and Pharmacological Action of Sanguisorba officinalis L.[J].Chinese Journal of Experimental Formulae,2016,22(20):189-195.)
[4]张向阳,刘春燕,贾丽霞,等.炒地榆炭及烘地榆炭对小鼠出血、凝血时间的影响[J].河南中医,2017,37(12):2109-2110.(ZHANG Xiangyang,LIU Chunyan,JIA Lixia,et al.Influence of Fried and Baked Diyutan(Radix sanguisorbae)on the Bleeding Time and Clotting Time in Mice[J].Henan Traditional Chinese Medicine,2017,37(12):2109-2110.)
[5]封烨,王金燕,南彦东,等.紫地榆提取物的止血作用[J].中成药,2017,39(10):2153-2155.(FENG Ye,WANG Jinyan,NAN Yandong,et al.Hemostatic Effect of Extracts from Ulmus Purpurea[J].Chinese Traditional Patent Medicine,2017,39(10):2153-2155.)
[6]陈园桃.自拟大黄地榆汤治疗上消化道出血72例[J].黑龙江中医药,2003(1):26-27.(CHEN Yuantao.Self-made Rhubarb Diyu Decoction for 72Cases of Upper Gastrointestinal Bleeding[J].Heilongjiang Journal of Traditional Chinese Medicine,2003(1):26-27.)
[7]赵元.地榆多糖的分离纯化及其对α-葡萄糖苷酶活性的抑制作用[D].济南:山东大学,2006.(ZHAO Yuan.Isolation and Purification of Polysaccharide from Ulmus and Its Inhibitory Effect on Glucosidase Activity[D].Jinan:Shandong University,2006.)
[8]耿玲,陈俊雅,李洪文,等.紫地榆提取物体外抗菌活性研究[J].大理学院学报,2014,13(10):14-17.(GENG Ling,CHEN Junya,LI Hongwen,et al.The Antibacterial Activity of Extracts from Geranium Strictipes R.Kunth in Vitro[J].Journal of Dali University,2014,13(10):14-17.)
[9]KIM S R,WON J H,KIM M R,et al.Antimicrobial Activity against Food Hazardous Microorganisms and Antimutagenicity against Salmonella serotype Typhimurium TA100of an Ethanol Extract from Snguisorba officinalis L.[J].Korean Journal of Food and Cookery Science,2011,27(4):17-26.
[10]LEE H A,HONG S H,OH H G,et al.Antibacterial Activity of Sanguisorba officinalis against Helicobacter pylori[J].Laboratory Animal Research,2010,26(3):257-263.
[11]CHEN Xiaolin,SHANG Fei,MENG Yajing,et al.Ethanol Extract of Sanguisorba officinalis L.Inhibits Biofilm Formation of Methicillin-Resistant Staphylococcus aureus in an Ica-Dependent Manner[J].Journal of Dairy Science,2015,98(12):8486-8491.
[12]周本宏,松长青,姜姗,等.地榆鞣质提取物的抗菌活性及对金黄色葡萄球菌的抑菌机制研究[J].中国药师,2016,19(3):464-469.(ZHOU Benhong,SONG Changqing,JIANG Shan,et al.Antibacterial Activity of Sanguisorba Tannin Extract and Mechanism of Antibacterial Effect on Staphylococcus aureus[J].China Pharmacist,2016,19(3):464-469.)
[13]张蕾,潘一鸣,宛春雷,等.狼爪瓦松提取物对伤寒杆菌和痢疾杆菌的抑菌活性机制[J].吉林大学学报(理学版),2017,55(6):1621-1625.(ZHANG Lei,PAN Yiming,WAN Chunlei,et al.Antibacterial Activities Mechanism of Extracts fromOrostachys cartilaginous A.Bor on Salmonella typhoon and Shigella dysenteric[J].Journal of Jilin University(Science Edition),2017,55(6):1621-1625.)
[14]张佳,王莹,张峰,等.滤纸片法测定黄花蒿提取物对霉菌的抑制活性[J].湖北农业科学,2009,48(5):1153-1154.(ZHANG Jia,WANG Ying,ZHANG Feng,et al.Mensurating the Antiseptic Activity of Extracts from Artemisia annua L.to Mold with the Filter Paper Method[J].Hubei Agricultural Sciences,2009,48(5):1153-1154.)
[15]孙延忠,曾洪梅,石义萍,等.武夷菌素对番茄灰霉菌(Bocrycis cinerea)的作用方式[J].植物病理学报,2003,33(5):434-438.(SUN Yanzhong,ZENG Hongmei,SHI Yiping,et al.Mode of Action of Wuyiencin on Bocrycis cinerea[J].Acta Phytopathologica Sinica,2003,33(5):434-438.)
[16]许维国,刘洋,刘多见,等.没食子酸抑菌活性分析[J].中国公共卫生,2012,28(10):1329-1331.(XU Weiguo,LIU Yang,LIU Duojian,et al.Antimicrobial Activity of Galic Acid[J].Chinese Journal of Public Health,2012,28(10):1329-1331.)
[17]倪建新,陈木水,林跃虹.远志属植物三萜皂苷及其生物活性研究进展[J].中药材,2011,34(2):317-319.(NIJianxin,CHEN Mushui,LIN Yuehong.Research Progress on Triterpenoid Saponins and Their Biological Activities in Polygalal[J].Journal of Chinese Medicinal Materials,2011,34(2):317-319.)
[18]胡焱,何淞,王瑶.齐墩果酸的抗菌作用研究进展[J].中国新药杂志,2017,26(3):304-308.(HU Yan,HE Song,WANG Yao.Progress in Researches of the Antibacterial Effect of Oleanolic Acid[J].Chinese Journal of New Drugs,2017,26(3):304-308.)
[19]LI Shiyou,ZHANG Zhizhen,CAIN A,et al.Antifungal Activity of Camptothecin,Trifolin,and Hyperoside Isolated fromCamptotheca acuminata[J].Journal of Agricultural and Food Chemistry,2005,53(1):32-37.
[20]LI Zilin,LIU Jincheng,HU Jing,et al.Protective Effects of Hyperoside against Human Umbilical Vein Endothelial Cell Damageinduced by Hydrogen Peroxide[J].Journal of Ethnopharmacology,2012,139(2):388-394.
[21]JURIKOVA T,ROP O,MLCEK J,et al.Phenolic Profile of Edible Honeysuckle Berries(Genus lonicera)and Their Biological Effects[J].Molecules,2011,17(1):61-79.
[22]张军,田子罡,王建华,等.有机酸抑菌分子机理研究进展[J].畜牧兽医学报,2011,42(3):323-328.(ZHANGJun,TIAN Zigang,WANG Jianhua,et al.Advances in Antimicrobial Molecular Mechanism of Organic Acid[J].Acta Veterinaria et Zootechnica Sinica,2011,42(3):323-328.)
[23]沈思,李孚杰,梅光明,等.茯苓皮三萜类物质含量的测定及其抑菌活性的研究[J].食品科学,2009,30(1):95-98.(SHEN Si,LI Fujie,MEI Guangming,et al.Sutdy on Content Determination and Antibacterial Effects of Triterpenes from Sclerotia of Poria cocos[J].Food Science,2009,30(1):95-98.)
[24]游庭活,刘凡,温露,等.黄酮类化合物抑菌作用研究进展[J].中国中药杂志,2013,38(21):3645-3650.(YOUTinghuo,LIU Fan,WEN Lu,et al.Research Progress on Antimicrobial Activities of Flavonoids[J].China Journal of Chinese Materia Medica,2013,38(21):3645-3650.)