全自动薄膜芯片法快速鉴定食品中3种牛源性成分
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摘要
为建立动物性食品中三种牛源性成分的检测体系,采用自动微流控薄膜芯片方法进行了研究。先将3种牛特定的DNA探针,固定于尼龙薄膜上,放入自动微流控薄膜芯片装置的微反应池。然后将生物素标记的含有靶DNA片段的PCR产物通过微流控系统模块加入反应池。PCR产物通过尼龙薄膜,经DNA互补作用捕获靶DNA片段,信号通过链霉亲和素连接碱性磷酸酶级联放大后显色出来。试验证明,本方法对牛源性成分检测的敏感性达到了1%的质量百分比,其稳定性和一致性可以满足实际应用的要求。
To establish the detection system of three kinds of bovine derived components in animal food,this paper presents our newly-developed auto-microfluidic thin-film chip method for screening of three kinds of bovine derived-materials. First,specific DNA probes for bovine derived-materials were immobilized on a square nylon thin-film. Then when the PCR products passed the square nylon thin-film,the target DNA fragments were captured,where the signal was visualized with streptavidin link-coupled alkaline phosphatase color development kit. Different animalderived-materials samples were tested. The results showed that the automatic film chip hybridization instrument provided good sensitivity and specificity. The repeatability was 100% and the sensitivity was 1%,which provides a high throughput,high sensitivity and high throughput detection method for bovine derived-materials.
To establish the detection system of three kinds of bovine derived components in animal food,this paper presents our newly-developed auto-microfluidic thin-film chip method for screening of three kinds of bovine derived-materials. First,specific DNA probes for bovine derived-materials were immobilized on a square nylon thin-film. Then when the PCR products passed the square nylon thin-film,the target DNA fragments were captured,where the signal was visualized with streptavidin link-coupled alkaline phosphatase color development kit. Different animalderived-materials samples were tested. The results showed that the automatic film chip hybridization instrument provided good sensitivity and specificity. The repeatability was 100% and the sensitivity was 1%,which provides a high throughput,high sensitivity and high throughput detection method for bovine derived-materials.
引文
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